Presence and bioavailability of bisphenol A in the uterus of rats and mice following single and repeated dietary administration at low doses

This research examined the distribution of low dietary doses of bisphenol A (BPA). When female rats received 50 mu g/kg C-14-BPA orally, radioactivity was distributed throughout the body, with especial presence in the uterus. Pre-treatment with estradiol or the estrogen antagonist ICI 182,780 significantly reduced radioactivity in the uterus. The majority of BPA at the uterus was determined to be aglycone (receptor-active) via GC-MS. Subsequently, mice given 0.5, 5, or 50 mu g/kg C-14-BPA showed more radioactivity in the uterus than in other non-metabolic tissues. When female mice received 1, 7. or 28 daily doses of 50 mu g/kg C-14-BPA, then were measured 24 h after the last dose, significantly more radioactivity was detected in the uterus, liver, and kidney following repeated doses. Collectively, these data provide evidence for the in vivo interaction of BPA with estrogen receptors. They also indicate elevated presence of BPA in reproductive tissues after repeated low doses. (C) 2014 Elsevier Inc. All rights reserved.