期刊
REPRODUCTIVE TOXICOLOGY
卷 34, 期 4, 页码 529-537出版社
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/j.reprotox.2012.08.001
关键词
Embryonic stem cells; Neural stem cells; Pulmonary fibroblasts; Electronic cigarettes; E-juice; Nicotine; Cytotoxicity; Tobacco-related disease
资金
- California Tobacco-Related Disease Research Program [XT-0118]
- California Institute for Regenerative Medicine [NE-A0005A-IE]
- UCR Academic Senate
- UCR Graduate Division
- STEM Pathway Project
- TRDRP [20FT-0084]
Electronic cigarettes (EC) and refill fluids are distributed with little information on their pre- and postnatal health effects. This study compares the cytotoxicity of EC refill fluids using embryonic and adult cells and examines the chemical characteristics of refill fluids using HPLC. Refill solutions were tested on human embryonic stem cells (hESC), mouse neural stem cells (mNSC), and human pulmonary fibroblasts (hPF) using the MTT assay, and NOAELs and IC(50)s were determined from dose-response curves. Spectral analysis was performed when products of the same flavor had different MU outcomes. hESC and mNSC were generally more sensitive to refill solutions than hPF. All products from one company were cytotoxic to hESC and mNSC, but non-cytotoxic to hPF. Cytotoxicity was not due to nicotine, but was correlated with the number and concentration of chemicals used to flavor fluids. Additional studies are needed to fully assess the prenatal effect of refill fluids. (C) 2012 Elsevier Inc. All rights reserved.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据