Article
Chemistry, Medicinal
Mirjam Luijten, Paul F. K. Wackers, Emiel Rorije, Jeroen L. A. Pennings, Harm J. Heusinkveld
Summary: The study found significant differences between in vitro and in vivo matching results, which could not be explained by physicochemical properties. However, pathway analyses indicated that the mechanisms perturbed in vitro are consistent with those perturbed in vivo in relevant matches.
CHEMICAL RESEARCH IN TOXICOLOGY
(2021)
Article
Biotechnology & Applied Microbiology
Vera A. van der Weijden, Meret Schmidhauser, Mayuko Kurome, Johannes Knubben, Veronika L. Floter, Eckhard Wolf, Susanne E. Ulbrich
Summary: Our study found that in vivo developed and in vitro produced embryos show similar transcriptome profiles during development. Shared canonical pathways in transitions from 4-cell to morula included oxidative phosphorylation and EIF2 signaling, while shared pathways from morula to hatched blastocyst included 14-3-3-mediated signaling, xenobiotic metabolism general signaling pathway, and NRF2-mediated oxidative stress response. Hatched blastocysts from in vitro fertilization-pipeline exhibited molecular pathways associated with lower developmental competence compared to in vivo developed embryos.
Article
Genetics & Heredity
Sarah Jane Appleby, Pavla Misica-Turner, Fleur Catherine Oback, Arindam Dhali, Zachariah Louis McLean, Bjorn Oback
Summary: Improving the efficiency of embryo cloning by enhancing epigenetic reprogramming during mitosis. It was found that this method can improve plating efficiency and the expression of pluripotency-associated genes, but mitotic donors cause ploidy errors during in vitro development that cannot be rescued.
FRONTIERS IN GENETICS
(2022)
Article
Environmental Sciences
Masahiro Akiyama, Yasuhiro Shinkai, Hiroto Yamakawa, Yun-Gi Kim, Yoshito Kumagai
Summary: Combined exposure to methylmercury (MeHg) and copper can increase the toxicity of MeHg and lead to higher mercury accumulation in the body. This study suggests that the sensitivity to MeHg can be increased by simultaneous exposure to certain electrophilic metals, particularly during pregnancy.
Article
Cell Biology
Elena Stocco, Silvia Barbon, Marco Mammana, Diletta Trojan, Alice Bianchin, Francesca Favaretto, Martina Contran, Giovanni Zambello, Andrea Vogliardi, Marta Confalonieri, Silvia Todros, Piero G. Pavan, Filippo Romanato, Maria Teresa Conconi, Veronica Macchi, Raffaele De Caro, Federico Rea, Andrea Porzionato
Summary: The study aimed to develop a new decellularized trachea from pig tissue and compare it with cryopreserved trachea for predictive analysis. The decellularization effectively removed Alpha-Gal epitopes, while cryopreserved samples retained some respiratory epithelial cells. Despite some limitations, both cryopreserved and decellularized trachea are viable options that require further investigation in comparative animal studies.
Article
Biology
Bolat Seisenov, Dulat Duimbayev, Nurlybay Kazhgaliyev, Talgat Abdrakhmanov, Alexandra Tegza, Rustem Abeldinov, Nadezhda Burambayeva, Alma Temirzhanova, Ivan Tegza, Zhomart Kemeshev, Assylbek Zhanabayev, Nurbolat Akhmetbekov, Marat Aisin, Kuandyk Zhugunissov, Arman Issimov
Summary: In vitro fertilization (IVF) technologies show promise in preserving endangered species. This study conducted an IVF experiment on the Kazakh Whiteheaded cattle, which has experienced a drastic population decline. The results compared the reproductive characteristics of this breed to Aberdeen Angus cows and found the Kazakh Whiteheaded cattle to have inferior reproductive abilities.
Article
Toxicology
Geronimo Matteo, Karen Leingartner, Andrea Rowan-Carroll, Matthew Meier, Andrew Williams, Marc A. Beal, Matthew Gagne, Reza Farmahin, Shamika Wickramasuriya, Anthony J. F. Reardon, Tara Barton-Maclaren, J. Christopher Corton, Carole L. Yauk, Ella Atlas
Summary: Since 2010, BPA has been replaced by alternative chemicals in Canada. However, many of these chemicals lack sufficient data for toxicological risk assessment. In this study, high-throughput transcriptomics was used to assess the hazards of BPA and 15 alternative chemicals in breast cancer cells. The results indicate that several BPA alternative chemicals may cause similar hazards at similar concentrations to BPA, highlighting the importance of transcriptomic profiling for risk assessment of data-poor chemicals.
TOXICOLOGICAL SCIENCES
(2023)
Article
Environmental Sciences
Katlin L. Bowman, Carl H. Lamborg, Alison M. Agather, Chad R. Hammerschmidt
Summary: This study found that plastic debris can alter the biogeochemical cycling of mercury in aquatic ecosystems, as microbial communities on plastic may contain mercury methylating microbes and mer genes.
MARINE POLLUTION BULLETIN
(2021)
Article
Agriculture, Dairy & Animal Science
Lydia K. Wooldridge, Jessica A. Keane, Michelle L. Rhoads, Alan D. Ealy
Summary: Research has shown that there are limitations in in vitro embryo production, but supplementing certain embryo factors may help overcome these limitations, thus improving the development and post-transfer competency of IVP embryos.
JOURNAL OF ANIMAL SCIENCE
(2022)
Review
Genetics & Heredity
Douglas M. Ruden
Summary: This article introduces the Frontiers Media family, which consists of over 200 journals, and provides an overview of the development of Frontiers in Genetics and Frontiers in Toxicogenomics. It lists several highly cited articles and discusses the future directions of these two journals.
FRONTIERS IN GENETICS
(2022)
Article
Reproductive Biology
Zachary K. Seekford, Lydia K. Wooldridge, Nicholas W. Dias, Claire L. Timlin, Alvaro F. Sales, Savannah L. Speckhart, Ky G. Pohler, Rebecca R. Cockrum, Vitor R. G. Mercadante, Alan D. Ealy
Summary: Supplementing IL6 during in vitro embryo development has a positive impact on the developmental trajectory of the embryo and fetus, resulting in pregnancies that more closely resemble those generated through timed artificial insemination.
Article
Environmental Sciences
Tania Charette, Danyel Bueno Dalto, Maikel Rosabal, J. Jacques Matte, Marc Amyot
Summary: This study found that although in vitro models showed that cooking fish muscle can significantly decrease the bioaccessibility of MeHg, it did not have the same effect in in vivo experiments. The research suggests that bioaccessibility is not suitable as a direct surrogate for evaluating the effect of cooking on MeHg availability in the body.
Article
Biochemistry & Molecular Biology
Elisabetta Damiani, Fiorenza Sella, Paola Astolfi, Roberta Galeazzi, Oliana Carnevali, Francesca Maradonna
Summary: The demand for organic UV filters as active components in sunscreen products has increased rapidly due to growing awareness of the hazards of UV radiation. However, their extensive use has raised concerns about their effects on aquatic organisms. This study used zebrafish to investigate the effects of exposure to a derivative of the popular UV filter octyl methoxycinnamate (OMC), called tempol-methoxycinnamate (TMC). The results showed that while OMC exposure increased stress and oxidative response genes, TMC had reduced toxicity and exhibited antioxidant activity. Furthermore, TMC and OMC showed different interactions with hormone receptors, suggesting TMC as a more environmentally friendly alternative UV filter.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Review
Cell Biology
Miaoci Zhang, Alice H. Reis, Mijo Simunovic
Summary: The molecular mechanisms of early human embryonic events have become a major focus of developmental biology research, as they have the potential to impact the treatment of pregnancy disorders and infertility. Recent progress in 3D culture of human embryonic stem cells has provided a new opportunity to study these critical events. In this review, we discuss the development of 3D models of the human embryo, known as embryoids, and their potential in studying various aspects of early human development.
SEMINARS IN CELL & DEVELOPMENTAL BIOLOGY
(2023)
Article
Pediatrics
Dorothea Wiegel, Christiane E. L. Dammann, Heber C. Nielsen
Summary: This study found that ErbB4-Jma significantly increased in fetal mouse ATII cells after E16.5. Through in silico analysis, four candidate splice factors were identified: FOX2, CUG/CELF1, TIAR, and HUB. Downregulation of the HUB gene in E17.5 ATII cells increased the levels of Jma isoform and Sftpb gene expression, while decreasing Jmb. These results suggest that the HUB gene plays a developmental regulatory role in ErbB4 AS in fetal mouse ATII cells, promoting ATII cell differentiation.
PEDIATRIC RESEARCH
(2022)
Article
Substance Abuse
Jeroen L. A. Pennings, Anne Havermans, Charlotte G. G. M. Pauwels, Erna J. Z. Krusemann, Wouter F. Visser, Reinskje Talhout
Summary: The presence of nicotine salt-containing e-liquids in the Netherlands market has increased significantly in recent years. Nicotine salts, due to their characteristics, make these e-liquids more addictive and attractive, particularly to young and inexperienced users. Policymakers are advised to regulate products containing nicotine salts.
Article
Substance Abuse
Jeroen L. A. Pennings, Geoffrey Ferris Wayne, Walther N. M. Klerx, Charlotte G. G. M. Pauwels, Reinskje Talhout
Summary: This study explores the impact of tobacco blend and design characteristics on the sensory experience of cigarette consumers. The findings suggest that while some variations in design are noticeable to consumers, many products on the market are not discernibly different, indicating that brand diversity may serve non-sensory purposes such as marketing.
Article
Substance Abuse
Ingrid M. E. Bakker-'t Hart, Frank Bakker, Jeroen L. A. Pennings, Naomi Weibolt, Selma Eising, Reinskje Talhout
Summary: This study examines the commonly used flavors and flavorings in waterpipe tobacco and related products, and presents a waterpipe tobacco flavor wheel for experts' reference. The analysis reveals that the majority of waterpipe tobacco products are flavored, with fruit flavors being the most prevalent. Herbal molasses and steam stones contain similar levels of flavorings, with lower levels compared to waterpipe tobacco. The flavorings in waterpipe products are mainly fruity and sweet, often combined with menthol/mint flavorings. Banning flavorings in all waterpipe products could be an effective strategy to reduce waterpipe smoking among youth.
Article
Pharmacology & Pharmacy
Marja Driessen, Suzanne van der Plas-Duivesteijn, Anne S. Kienhuis, Evert-Jan van den Brandhof, Marianne Roodbergen, Bob van de Water, Herman P. Spaink, Magnus Palmblad, Leo T. M. van der Ven, Jeroen L. A. Pennings
Summary: The zebrafish embryo is a promising model for studying hepatotoxicity and could potentially reduce the use of rodents in such assessments. Proteomics analysis identified a set of potential protein markers for detecting adverse liver responses.
Article
Multidisciplinary Sciences
Andreja Novak, Jeroen L. A. Pennings, Larissa van der Maas, Hugo D. Meiring, Irene Ludwig, Saertje Verkoeijen, Victor Rutten, Femke Broere, Arjen Sloots
Summary: Stimulation of a canine monocyte/macrophage cell line with Leptospira strains and vaccines revealed differentially expressed genes and proteins, suggesting potential biomarkers for Leptospira vaccine quality control.
SCIENTIFIC REPORTS
(2022)
Review
Substance Abuse
Anette K. Bolling, Nadja Mallock, Efthimios Zervas, Stephanie Caille-Garnier, Thibault Mansuy, Cecile Michel, Jeroen L. A. Pennings, Thomas Schulz, Per E. Schwarze, Renata Solimini, Jean-Pol Tassin, Constantine Vardavas, Miguel Merino, Charlotte G. G. M. Pauwels, Lotte E. van Nierop, Claude Lambre, Anne Havermans
Summary: The study found significant limitations in the methodology used by international tobacco companies in their reports, rendering them unsuitable for determining whether priority additives should be banned in tobacco products according to the Tobacco Products Directive.
TOBACCO PREVENTION & CESSATION
(2022)
Review
Substance Abuse
Anne Havermans, Nadja Mallock, Efthimios Zervas, Thibault Mansuy, Jeroen L. A. Pennings, Thomas Schulz, Per E. Schwarze, Renata Solimini, Jean -Pol Tassin, Constantine I. Vardavas, Miguel Merino, Charlotte G. G. M. Pauwels, Lotte E. van Nierop, Claude Lambre, Anette K. Bolling
Summary: This study evaluates the 15 priority additives in the European Union Tobacco Products Directive. The industry reports were found to be insufficient and methodologically limited, and the issues of toxicity, addictiveness, and attractiveness of the additives were not adequately addressed. Alternative approaches such as the precautionary principle are recommended.
TOBACCO PREVENTION & CESSATION
(2022)
Article
Environmental Sciences
Victoria C. de Leeuw, Conny T. M. van Oostrom, Paul F. K. Wackers, Jeroen L. A. Pennings, Hennie M. Hodemaekers, Aldert H. Piersma, Ellen V. S. Hessel
Summary: There is an increasing recognition of the limitations of using animals for compound-induced developmental neurotoxicity (DNT) testing. Human stem cell-based models, such as the human neural progenitor test (hNPT), play a pivotal role in studying DNT as they can replicate relevant processes in human brain development. This study characterized the differentiation of neural progenitor cells over time and identified neurodevelopmental processes sensitive to compound exposure using transcriptomics.
Article
Reproductive Biology
Laura M. M. Samrani, Jeroen L. A. Pennings, Nina Hallmark, Remi Bars, Helen Tinwell, Marc Pallardy, Aldert H. Piersma
Summary: The study investigates the effect of exposure duration on gene expression changes in the all-trans retinoic acid (ATRA) signaling pathway in zebrafish embryotoxicity test (ZET). It is found that the highest magnitudes of gene expression regulation occur after 2-24 hrs exposure, with the optimal response at 4 hrs. Early assessment of gene expression regulation after exposure onset in ZET could be optimal for predicting developmental toxicity.
REPRODUCTIVE TOXICOLOGY
(2023)
Article
Substance Abuse
Jeroen L. A. Pennings, Anne Havermans, Erna J. Z. Krusemann, Dion Zijtveld, Eva H. W. Huiberts, Peter M. J. Bos, Erna Schenk, Wouter F. Visser, Ingrid M. EBakker-'t Hart, Yvonne C. M. Staal, Reinskje Talhout
Summary: The Dutch government plans to only allow tobacco flavors in e-liquids to discourage e-cigarette use among youth. Researchers have compiled a restrictive list of 16 tobacco flavorings to implement this policy.
Review
Chemistry, Medicinal
Christina H. J. Veltman, Jeroen L. A. Pennings, Bob van de Water, Mirjam Luijten
Summary: Nongenotoxic (NGTX) carcinogens induce cancer through mechanisms other than direct DNA damage, primarily involving the induction of oxidative stress leading to regenerative proliferation. However, current carcinogenicity assessment methods mainly focus on genetic toxicity, which may overlook NGTX carcinogens. To improve the predictivity of carcinogenicity assessment, a mechanism-based approach such as the adverse outcome pathway (AOP) network for chemically induced oxidative stress leading to (NGTX) carcinogenesis is proposed. This AOP network considers the role of oxidative stress in cancer development and explores the mechanisms and biological effects of chemical-induced oxidative damage. Developing AOP networks relevant to human carcinogenesis will facilitate a transition to a mechanism-based, human-relevant carcinogenicity assessment with reduced reliance on laboratory animals.
CHEMICAL RESEARCH IN TOXICOLOGY
(2023)
Editorial Material
Environmental Sciences
Abebe G. Aberra, Aesha Mokashi, Serena J. Santoni, Charles T. Tilander, Patricia Cirone, Bruce Duncan, Elaine M. Faustman
INTEGRATED ENVIRONMENTAL ASSESSMENT AND MANAGEMENT
(2023)
Article
Neurosciences
Faycal Guedj, Elise Kane, Lauren A. Bishop, Jeroen L. A. Pennings, Yann Herault, Diana W. Bianchi
Summary: The effectiveness of the Ts65Dn mouse model in improving neurocognition in Down syndrome has not been successfully translated to humans. The Ts66Yah mouse model, which carries an extra chromosome and identical segmental trisomy as Ts65Dn, was used to investigate the underlying factors.
BIOLOGICAL PSYCHIATRY
(2023)
Article
Substance Abuse
Charlotte G. G. M. Pauwels, Wouter F. Visser, Jeroen L. A. Pennings, Ernesto P. Baloe, Arnout P. T. Hartendorp, Loes van Tiel, Mirjam van Mourik, Wim Vaessen, Sanne Boesveldt, Reinskje Talhout
Summary: This study aimed to determine whether nicotine salts increase sensory appeal at lower concentrations and compared the inhalation intensity of both types of e-liquids. The results showed that nicotine salts did not significantly affect sensory appeal, harshness, and puffing intensity compared to free-based nicotine.
DRUG AND ALCOHOL DEPENDENCE
(2023)
Article
Substance Abuse
Jeroen L. A. Pennings, Charlotte G. G. M. Pauwels, Erna Schenk, Reinskje Talhout
Summary: Cigarillos have emerged as a substitute for flavored cigarettes, which are prohibited in the European Union, and they are also cheaper. To gain a better understanding of the cigarillo market, we analyzed product data from manufacturers via the EU-CEG system.
TOBACCO INDUCED DISEASES
(2023)
Article
Reproductive Biology
Lei Gong, Siyi Jiang, Jia Tian, Yong Li, Wansu Yu, Lubo Zhang, Daliao Xiao
Summary: Exposure to gestational diabetes mellitus during pregnancy can lead to the development of a neonatal brain hypoxia/ischemia-sensitive phenotype. The PTEN/AKT/mTOR/autophagy signaling pathway plays a role in this process.
REPRODUCTIVE TOXICOLOGY
(2024)
Article
Reproductive Biology
Sudipta Dutta, Kirthiram K. Sivakumar, John W. Erwin, Jone A. Stanley, Joe A. Arosh, Robert J. Taylor, Sakhila K. Banu
Summary: This study found that exposure to hexavalent chromium disrupts follicle development and causes apoptosis by altering epigenetic marks in the ovary.
REPRODUCTIVE TOXICOLOGY
(2024)
Article
Reproductive Biology
Yuxin Li, Xianzhi He, Bin Sun, Nannan Hu, Jiamin Li, Ruolan You, Feiyan Tao, Lei Fang, Yuanyuan Li, Qingfeng Zhai
Summary: This study investigated the toxic effects and mechanism of beta-CYP and EMB on the reproductive system of female rats. The results showed that exposure to these pesticides led to changes in hormone and gene expression in the hypothalamic-pituitary-ovarian axis, as well as alterations in oxidative damage indicators. The findings support the evaluation of the adverse effects of pesticide exposure on reproductive health in greenhouse operations.
REPRODUCTIVE TOXICOLOGY
(2024)
Article
Reproductive Biology
Ran Lee, Won-Young Lee, Hyun-Jung Park
Summary: This study investigates the toxicity of diuron on fetal mouse testes and finds that diuron has a negative impact on the development of fetal Leydig cells, while not affecting germ cells and Sertoli cells.
REPRODUCTIVE TOXICOLOGY
(2024)