期刊
REPRODUCTIVE TOXICOLOGY
卷 27, 期 1, 页码 14-21出版社
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/j.reprotox.2008.11.052
关键词
Apoptosis; HSV-infection; HSV1-TK; Spermatogenesis; Transgenic rat
资金
- Ministry of Education, Culture, Sports, Science and Technology
- Grants-in-Aid for Scientific Research (B) [02640578, 06454019]
- Institute of Science and Technology, Meiji University
- Grant-in-Aid for Research (A)
- Ministry of Education, Culture, Sports, Science and Technology of Japan
- Grants-in-Aid for Scientific Research [06454019, 02640578] Funding Source: KAKEN
HSV type 1 thymidine kinase (HSV1-TK)-introduced transgenic rodents and HSV-infected humans were reported to suffer male infertility. The present study aimed to find novel clues to clarify the cause of HSV1-TK-induced male infertility using an HSV1-tk transgenic rat line. Two truncated HSV1-TK proteins, 37 and 39 kDa, were produced and accumulated in the round spermatids, and their transcription initiation site was identified for the first time at the 65 base downstream of the translation start point of the full-length 43 kDa HSV1-TK. Spermatozoa from those young transgenic rats showed malformed heads, looped tails, and missing cell membrane in heads and tails. Furthermore, age-dependent germ cell loss was observed. TUNEL assay suggested that this germ cell loss is caused by increased apoptotic germ cell death. These results suggest that the expression of HSV1-TK in testes brings about not only abnormal spermiogenesis but also a loss of germ cells due to apoptosis. These findings could provide a novel clue to elucidate the molecular mechanism underlying male infertility in transgenic animals and HSV-infected patients. (C) 2008 Elsevier Inc. All rights reserved.
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