期刊
REPRODUCTIVE BIOMEDICINE ONLINE
卷 20, 期 3, 页码 320-323出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.rbmo.2009.12.004
关键词
annexin V microbeads; apoptosis; DNA fragmentation; ICSI; MACS; spermatozoa
Magnetic activated cell sorting (MACS) with annexin V microbeads recognizes externalized phosphatidylserine (PS) residues on the surface of apoptotic spermatozoa. The successful use of this novel technique applied to a highly apoptotic semen sample before performing intracytoplasmic sperm injection (ICSI) is reported here. The use of annexin V microbeads for selecting non-apoptotic spermatozoa seems to reduce the percentage of altered cells, improving the chance of pregnancy after ICSI. (C) 2009, Reproductive Healthcare Ltd. Published by Elsevier Ltd. All rights reserved.
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