Article
Cardiac & Cardiovascular Systems
Adela Herraiz-Martinez, Carmen Tarifa, Veronica Jimenez-Sabado, Anna Llach, Hector Godoy-Marin, Hildegard Colino, Carme Nolla-Colomer, Sergi Casabella, Paloma Izquierdo-Castro, Ivan Benitez, Raul Benitez, Elena Rosello-Diez, Enrique Rodriguez-Font, Xavier Vinolas, Francisco Ciruela, Juan Cinca, Leif Hove-Madsen
Summary: Sex-dependent disturbances in calcium homeostasis in atrial fibrillation result in increased spontaneous sarcoplasmic reticulum calcium release-induced electrical activity in women, while men exhibit diminished L-type calcium current density. Females with AF have more calcium spark sites and longer sparks, leading to higher incidence of transient inward currents and afterdepolarizations.
CARDIOVASCULAR RESEARCH
(2022)
Article
Cell Biology
Alexia Tiberi, Nicola Maria Carucci, Giovanna Testa, Caterina Rizzi, Paola Pacifico, Giulia Borgonovo, Ivan Arisi, Mara D'Onofrio, Rossella Brandi, Wen-Biao Gan, Simona Capsoni, Antonino Cattaneo
Summary: Nerve growth factor (NGF) has been shown to have a critical role in neuronal physiology. However, this study reveals that astrocytes in the central nervous system are also affected by changes in NGF levels, leading to astrocytic atrophy and proinflammatory responses. Additionally, inhibition of NGF leads to increased calcium activity in layer I astrocytes, which can be reversed by acute administration of NGF.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2023)
Article
Cell Biology
Anna Pensalfini, Abdul Rahim Umar, Charles Glabe, Ian Parker, Ghanim Ullah, Angelo Demuro
Summary: In this study, brain extracts from AD patients were injected into Xenopus oocytes, and it was found that only the extracts from AD patients containing soluble A beta oligomers triggered intracellular Ca2+ events. This supports the hypothesis that A beta oligomers may be the toxic agents responsible for neuronal dysfunction and death in AD.
Article
Biochemistry & Molecular Biology
Amanda A. de Oliveira, Valentina O. Mendoza, Fernanda Priviero, R. Clinton Webb, Kenia P. Nunes
Summary: This study found lower levels of HSP70 in the aortas of middle-aged rats, which may lead to a decline in vascular responses to alpha-1 adrenergic stimulation. Functional HSP70 is necessary for proper calcium handling. Additionally, HSP70 inhibitors may result in increased levels of reactive oxygen species in the aortic rings of middle-aged animals.
Article
Biochemistry & Molecular Biology
Natasja A. Otto, Joe M. Butler, Alex R. Schuurman, Xanthe Brands, Bastiaan W. Haak, Augustijn M. Klarenbeek, Michel van Weeghel, Riekelt H. Houtkooper, Marja E. Jakobs, Daniel R. Faber, Alex F. de Vos, W. Joost Wiersinga, Brendon P. Scicluna, Tom van der Poll
Summary: This study investigated the metabolic profile of blood monocytes in patients with community-acquired pneumonia (CAP), and found upregulation of genes involved in glycolysis. Monocytes from CAP patients had elevated levels of intracellular pyruvate and the TCA cycle intermediate alpha-ketoglutarate, and showed reduced cytokine production capacity upon LPS stimulation. Intracellular pyruvate concentration positively correlated with IL-18 and IL-10 levels released by CAP patients' monocytes. These findings suggest that elevated intracellular pyruvate levels may partially maintain cytokine production capacity in monocytes from CAP patients.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR BASIS OF DISEASE
(2022)
Article
Andrology
Faith T. Moichela, Gbenga A. Adefolaju, Ralf R. Henkel, Chinyerum S. Opuwari
Summary: The study found that the aqueous leaf extract of Moringa oleifera has antioxidant properties that can reduce sperm reactive oxygen species generation, DNA fragmentation, and acrosome reaction, while maintaining sperm capacitation and vitality. Further research is needed to confirm the impact of Moringa oleifera leaf extract on fertility potential.
Article
Biochemistry & Molecular Biology
Nan-Nan Yu, Wirinthip Ketya, Eun-Ha Choi, Gyungsoon Park
Summary: This study evaluated the potential of a non-thermal atmospheric pressure plasma jet to improve cellulolytic enzyme production efficiency. The results showed that plasma treatment significantly increased the activity and protein concentration of cellulolytic enzymes. Furthermore, plasma treatment promoted the transcription and secretion of cellulolytic enzymes by enhancing the intracellular levels of NO and Ca2+.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Veterinary Sciences
Ivan Yanez-Ortiz, Jaime Catalan, Ariadna Delgado-Bermudez, Augusto Carluccio, Jordi Miro, Marc Yeste
Summary: The addition of different concentrations of reduced glutathione (GSH) to fresh donkey sperm affects their cryotolerance, with treatments containing 8 mM and 10 mM GSH showing significant differences compared to the control. While GSH did not affect membrane integrity or superoxide levels, it did reduce the percentages of viable sperm with high ROS levels. This suggests that the antioxidant capacity of GSH in donkey sperm may have limitations in improving resilience to freeze-thawing.
VETERINARY SCIENCES
(2021)
Article
Biochemistry & Molecular Biology
Simone Fenk, Elizaveta Melnikova, Anastasia A. Anashkina, Yuri M. Poluektov, Pavel Zaripov, Vladimir A. Mitkevich, Yaroslav Tkachev, Lars Kaestner, Giampaolo Minetti, Heimo Mairbaeurl, Jeroen S. Goede, Alexander A. Makarov, Irina Yu Petrushanko, Anna Bogdanova
Summary: This study identifies an adaptive mechanism in red blood cells where the levels of intraerythrocytic GSH increase upon deoxygenation, providing an advanced antioxidant defense. This effect is regulated by the oxygen-dependent modulation of Hb levels and involves the non-covalent binding of GSH to oxy-Hb. The findings suggest that red blood cells can respond immediately to oxidative challenges by adjusting GSH levels upon deoxygenation.
Article
Biochemistry & Molecular Biology
Seon Ae Jeong, Jisoo Song, Jiyeon Ham, Garam An, Gwonhwa Song, Whasun Lim
Summary: This study found that tetraconazole, as a fungicide, not only directly affects plants and fruits, but also indirectly affects milk production in cows. Tetraconazole induces apoptosis, inhibits cell survival and proliferation, and disrupts calcium homeostasis and mitochondrial function in bovine mammary epithelial cells (MAC-T). It also alters the expression of proliferation-related proteins and signaling pathways, as well as impairs autophagy functioning in MAC-T cells. These findings suggest that tetraconazole exposure can lead to a failure of milk production in bovine mammary epithelial cells.
PESTICIDE BIOCHEMISTRY AND PHYSIOLOGY
(2023)
Article
Nutrition & Dietetics
Lara S. Boerenkamp, Birgit L. M. G. Gijsbers, Erik-Jan Ververs, Eva M. S. Pijpers, Bart Spaetgens, Aniek de Coninck, Wilfred T. V. Germeraad, Will K. W. H. Wodzig, Lotte Wieten, Gwendolyn N. Y. van Gorkom, Catharina H. M. J. van Elssen
Summary: This study investigated the levels of serum and intracellular vitamin C in hospitalized COVID-19 patients and found lower serum vitamin C levels in severe and critical cases. The study suggests that intracellular vitamin C levels decrease during COVID-19 infection, while serum levels remain relatively stable. These findings highlight the importance of further research on the role and mechanisms of intracellular vitamin C in COVID-19 infection.
Article
Multidisciplinary Sciences
Emma Cavarocchi, Camille Sayou, Patrick Lores, Caroline Cazin, Laurence Stouvenel, Elma El Khouri, Charles Coutton, Zine-Eddine Kherraf, Catherine Patrat, Jerome Govin, Nicolas Thierry-Mieg, Marjorie Whitfield, Pierre F. Ray, Emmanuel Dulioust, Aminata Toure
Summary: This study identified gene mutations associated with fertility, which affect sperm motility and capacitation. The mutations were found to impair the signaling pathways involved in the capacitation process, and a protein related to sperm motility and capacitation was also discovered.
Article
Biochemistry & Molecular Biology
Ken Yokawa, Yutaka Kodama
Summary: The new green light-excitable FRET system utilizing an mKO2/mCherry pair can effectively monitor changes in cytosolic free calcium concentration in plant cells, offering a solution to avoid unwanted blue light responses.
PLANT SIGNALING & BEHAVIOR
(2021)
Article
Cardiac & Cardiovascular Systems
Alessio Lissoni, Paco Hulpiau, Tania Martins-Marques, Nan Wang, Geert Bultynck, Rainer Schulz, Katja Witschas, Henrique Girao, Maarten De Smet, Luc Leybaert
Summary: The research revealed that the activation of ryanodine receptors (RyRs) triggers unitary currents with a single-channel conductance of around 220 pS, which are significantly reduced by knocking down connexin 43 (Cx43). The study also showed that both RyR activation and intracellular Ca2+ elevation are necessary for the opening of Cx43 hemichannels.
CARDIOVASCULAR RESEARCH
(2021)
Article
Medicine, General & Internal
Carla Sala, Marta Rescaldani, Elisa Gherbesi, Gianni Bolla, Cesare Cuspidi, Massimiliano Ruscica, Stefano Carugo
Summary: The present study found that chronic aldosterone excess impairs the platelet nitric oxide/cGMP pathway in humans, possibly due to potassium depletion caused by aldosterone.
JOURNAL OF CLINICAL MEDICINE
(2023)
Article
Biochemistry & Molecular Biology
Maria Patron, Veronica Granatiero, Javier Espino, Rosario Rizzuto, Diego De Stefani
CELL DEATH AND DIFFERENTIATION
(2019)
Article
Biochemistry & Molecular Biology
Javier Espino, Maria Macedo, Graciela Lozano, Agueda Ortiz, Carmina Rodriguez, Ana B. Rodriguez, Ignacio Bejarano
Article
Biochemistry & Molecular Biology
Elena Fernandez-Delgado, Felipe de la Cruz-Martinez, Carmen Galan, Lourdes Franco, Javier Espino, Emilio Vinuelas-Zahinos, Francisco Luna-Giles, Ignacio Bejarano
JOURNAL OF INORGANIC BIOCHEMISTRY
(2020)
Article
Nutrition & Dietetics
Ivan Cruz-Chamorro, Nuria Alvarez-Sanchez, Guillermo Santos-Sanchez, Justo Pedroche, Maria-Soledad Fernandez-Pachon, Francisco Millan, Maria Carmen Millan-Linares, Patricia Judith Lardone, Ignacio Bejarano, Juan Miguel Guerrero, Antonio Carrillo-Vico
Article
Multidisciplinary Sciences
Javier Espino, Elena Fernandez-Delgado, Samuel Estirado, Felipe de la Cruz-Martinez, Sergio Villa-Carballar, Emilio Vinuelas-Zahinos, Francisco Luna-Giles, Jose A. Pariente
SCIENTIFIC REPORTS
(2020)
Review
Biochemistry & Molecular Biology
Patricia Cosme, Ana B. Rodriguez, Javier Espino, Maria Garrido
Article
Chemistry, Medicinal
Silvia Gutierrez-Tarrino, Javier Espino, Francisco Luna-Giles, Ana B. Rodriguez, Jose A. Pariente, Emilio Vinuelas-Zahinos
Summary: Research on anticancer coordination complexes has led to the development of important drugs like cisplatin, carboplatin, and oxaliplatin, but with high toxicity and side effects. New types of platinum and palladium complexes have emerged as potential anticancer drugs, with Pt(II) complexes showing strong cytotoxicity against various tumor cell lines, while Pd(II) complexes exhibit weaker antitumor effects.
Article
Biochemistry & Molecular Biology
Elena Fernandez-Delgado, Samuel Estirado, Javier Espino, Emilio Vinuelas-Zahinos, Francisco Luna-Giles, Ana B. Rodriguez Moratinos, Jose A. Pariente
Summary: One of the commonly used strategies for drug development is the coordination of bioactive ligands to transition metals. In this study, four Pt(II) complexes were synthesized and characterized, and their anticancer abilities were investigated in different cancer cell lines. The results showed that modulating the lipophilicity of the ligands can help improve the cytotoxic effect of the metal complexes.
JOURNAL OF INORGANIC BIOCHEMISTRY
(2022)
Article
Biochemistry & Molecular Biology
Guillermo Santos-Sanchez, Eduardo Ponce-Espana, Juan Carlos Lopez, Nuria Alvarez-Sanchez, Ana Isabel Alvarez-Lopez, Justo Pedroche, Francisco Millan, Maria Carmen Millan-Linares, Patricia Judith Lardone, Ignacio Bejarano, Ivan Cruz-Chamorro, Antonio Carrillo-Vico
Summary: Anxiety is a prevalent psychiatric disorder worldwide, causing economic burden. Commercial anxiolytic treatments have limitations and high costs, leading to a search for natural and safer alternatives. This study found that lupin protein hydrolysate (LPH) has anxiolytic effects and could potentially be used in nutritional therapies for anxiety.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Samuel Estirado, Elena Fernandez-Delgado, Emilio Vinuelas-Zahinos, Francisco Luna-Giles, Ana B. Rodriguez, Jose A. Pariente, Javier Espino
Summary: This study evaluated the antitumoral potential of PtDPhPzTn against TNBC cells, showing enhanced cytotoxicity compared to cisplatin and PtPzTn, inducing apoptosis, causing S phase arrest, and impairing cell migration. Melatonin was found to enhance the anticancer effects of PtDPhPzTn, increasing apoptosis and inhibiting cell migration when used together.
Article
Biology
Ignacio Bejarano, Monica Dorado-Silva, Helia Sarmiento-Soto, Nuria Alvarez-Sanchez, Patricia Judith Lardone, Juan Miguel Guerrero, Pascual Sanchez-Martin, Antonio Carrillo-Vico
Summary: Despite advances in assisted reproduction technology, success rates remain low. The study found that the expression of GPX3 in cumulus cells was significantly reduced in embryos that successfully implanted, suggesting its potential as a poor prognostic indicator of implantation success.
Article
Pharmacology & Pharmacy
Elena Fernandez-Delgado, Samuel Estirado, Ana B. Rodriguez, Francisco Luna-Giles, Emilio Vinuelas-Zahinos, Javier Espino, Jose Antonio Pariente
Summary: Six squared-planar Pd(II) complexes with thiazine and thiazoline ligands were synthesized and characterized. Cytotoxicity assay showed that complexes with phenyl substitutions had the lowest IC50 values and induced apoptosis. The presence of bulky substitutions on the ligands, such as phenyl groups, may influence the cytotoxicity of the synthesized chemotherapeutic agents.
