Article
Endocrinology & Metabolism
Yaoxuan Li, Shikai Wang, Dawen Li, Yueyue Huang, Haifang Liu, Xiaohui Zhang, Jie Qin, Xianbao Mao, Zhengda Li, Liangshi Chen, Pingpin Wei, Wen Shi, Lintao Xue
Summary: The study found that short-interval second ejaculation on the day of oocyte retrieval can significantly improve sperm quality and embryo development in oligoasthenozoospermic males receiving intracytoplasmic sperm injection (ICSI) treatment, suggesting a non-invasive and effective strategy for improving ICSI outcomes.
FRONTIERS IN ENDOCRINOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Olga Tepla, Zinovij Topurko, Simona Jirsova, Martina Moosova, Eva Fajmonova, Radek Cabela, Katerina Komrskova, Irena Kratochvilova, Jaromir Masata
Summary: The aim of this study was to evaluate the efficiency of using meiotic spindle (MS) visibility and relative position to the polar body (PB) as indicators of oocyte maturation in order to optimize intracytoplasmic sperm injection (ICSI) timing. The visualization of MS influenced the pregnancy rate (PR) for patients over 35 years, but did not affect PR for patients under 35 years. Visualization of MS appears to be a useful parameter for assessment of oocyte maturity and ICSI timing for patients older than 35.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Obstetrics & Gynecology
Zhenxing Liu, Qingsong Xi, Lixia Zhu, Xue Yang, Lei Jin, Jiarui Wang, Tao Zhang, Xiaopei Zhou, Dazhi Zhang, Xuejie Peng, Yalin Luo, Zhou Li, Xianqin Zhang
Summary: The study identified two heterozygous missense mutations in the TUBB8 gene that resulted in oocytes with large polar bodies and fertilization failure in two patients. Bioinformatics analysis showed that these mutations are harmful.
REPRODUCTIVE SCIENCES
(2021)
Article
Endocrinology & Metabolism
Camille Robin, Audrey Uk, Christine Decanter, Helene Behal, Pierre Collinet, Chrystele Rubod, Anne-Laure Barbotin, Geoffroy Robin
Summary: The study aimed to evaluate the relationship between endometriosis and oocyte morphology after controlled ovarian stimulation for intracytoplasmic sperm injection (ICSI) on a large oocyte cohort, finding that endometriosis does not have a negative impact on oocytes' morphology in IVF-ICSI.
REPRODUCTIVE BIOLOGY AND ENDOCRINOLOGY
(2021)
Article
Reproductive Biology
Ana P. Flores Bragulat, Isabel Ortiz, Jaime Catalan, Jesus Dorado, Manuel Hidalgo, Luis Losinno, Marta Dordas-Perpinya, Ivan Yanez-Ortiz, Iris Martinez-Rodero, Jordi Miro, Andres Gambini
Summary: This study investigated the effect of supplementation with jenny preovulatory follicular fluid (PFF) on oocyte developmental competence in donkeys. The results showed that IVM media supplemented with FBS or donkey PFF can promote nuclear maturation and early embryo development in donkeys. This study is the first report of ICSI, time-lapse imaging, and in vitro blastocyst production in donkeys.
Article
Obstetrics & Gynecology
Takashi Morimoto, Tomohiro Maekawa, Shimpei Mizuta, Hidehiko Matsubayashi, Takumi Takeuchi, Yutaka Hata, Tomomoto Ishikawa
Summary: During the intracytoplasmic sperm injection (ICSI) procedure, unintentional membrane rupture (UMR) often leads to oocyte degeneration. The study showed that the ICSI Position Detector (IPD) can be helpful in identifying the best puncture location to prevent UMR and improve the success of the procedure.
REPRODUCTIVE BIOMEDICINE ONLINE
(2023)
Article
Multidisciplinary Sciences
Miriam A. Genuth, Yasuhiro Kojima, Doerthe Juelich, Hisanori Kiryu, Scott A. Holley
Summary: Embryonic development involves orderly cell state transitions based on noisy molecular processes. Using single-cell RNA sequencing and in vivo cell tracking data, we defined gene expression and cell motion states. Both states were mapped onto embryos and the dynamics of state transitions were studied using cell motion states. The reproducible pattern of cell motion states and bilateral symmetry arise from temporal averaging, while individual embryos exhibit transient deviations forming left-right asymmetries in collective cell motion. Collective cell behavior can be a source of asymmetry rather than a buffer against noisy individual cell behavior.
Article
Endocrinology & Metabolism
Jing Ling Ruan, Shan Shan Liang, Jia Ping Pan, Zhi Qin Chen, Xiao Ming Teng
Summary: This retrospective study found that artificial oocyte activation (AOA) with calcium ionophore ionomycin can significantly improve the reproductive outcomes of patients with previous fertilization failure and poor embryo development after intracytoplasmic sperm injection (ICSI). AOA can increase fertilization rate, embryo quality, implantation rate, clinical pregnancy rate, and live birth rate, while decreasing miscarriage rate. The study suggests that AOA is an effective and safe treatment option for some infertility patients.
FRONTIERS IN ENDOCRINOLOGY
(2023)
Article
Environmental Sciences
Piotr Balazy, Marti J. Anderson, Maciej Chelchowski, Maria Wlodarska-Kowalczuk, Piotr Kuklinski, Jorgen Berge
Summary: The study investigated scavenger activity during both Arctic polar night and day in a high Arctic fjord. The results showed significant differences in scavenger assemblages between winter and summer, as well as in response to different bait types. The consumption rates of bait were found to be slow in general, with only slight differences between the two seasons.
FRONTIERS IN MARINE SCIENCE
(2021)
Article
Genetics & Heredity
Jose Buratini, Clarissa Moutier, Maria Cristina Guglielmo, Diana Turchi, Robert Webb, Elena De Ponti, Mario Mignini Renzini, Mariabeatrice Dal Canto
Summary: This study aimed to assess the effects of oocyte central granularity and its underlying endocrine environment on the developmental competence of dysmorphic and morphologically normal oocytes. A retrospective cohort study was conducted with 1,082 patients undergoing autologous ICSI cycles. Results showed that CG oocytes had severely compromised developmental competence and a lower live birth rate compared to NCG and N oocytes. Maternal age should be considered when deciding whether to use CG oocytes in ICSI cycles.
JOURNAL OF ASSISTED REPRODUCTION AND GENETICS
(2023)
Article
Obstetrics & Gynecology
Amanda Setti, Daniela Braga, Patricia Guilherme, Assumpto Iaconelli, Edson Borges
Summary: This study found that increasing oocyte immaturity rate is associated with delayed cell cleavage and blastulation. These findings highlight the importance of TLI for identifying and deselecting slow-growing embryos for transfer in cycles with high oocyte immaturity rate.
REPRODUCTIVE BIOMEDICINE ONLINE
(2022)
Article
Obstetrics & Gynecology
Azam Govahi, Fatemehsadat Amjadi, Mohammad-Hossein Nasr-Esfahani, Ehsan Raoufi, Mehdi Mehdizadeh
Summary: This study aimed to investigate a more accurate and non-invasive method in embryo evaluation by combining time-lapse morphokinetic parameters and cumulus cells transcriptomic profile. Different groups of embryos showed distinct differences in both time-lapse parameters and transcriptomic profiles, indicating that this combined approach could improve the accuracy of embryo selection and pregnancy rate in ART clinics.
REPRODUCTIVE SCIENCES
(2022)
Article
Obstetrics & Gynecology
Amanda Souza Setti, Gabriela Halpern, Daniela Paes de Almeida Ferreira Braga, Assumpto Iaconelli Jr, Edson Borges Jr
Summary: Maternal lifestyle factors, such as smoking, alcohol consumption, refined sugar, and artificial sweeteners, have a significant impact on the incidence of oocyte dimorphisms and outcomes of intracytoplasmic sperm injection (ICSI) cycles. These habits are positively associated with oocyte dimorphisms and negatively associated with ovarian stimulation response, embryo development, clinical pregnancy rates, and live birth rates. The associations are dose-dependent.
REPRODUCTIVE BIOMEDICINE ONLINE
(2022)
Article
Reproductive Biology
Brandon A. Wyse, Noga Fuchs Weizman, Janice Montbriand, Rima Kharonsky, Ran Antes, Rina Abramov, Svetlana Madjunkova, Clifford L. Librach
Summary: Different IVF-ICSI workflows have varying effects on patients depending on their primary infertility diagnosis, highlighting the importance of tailoring the workflow to individual patients. This study emphasizes the impact of IVF laboratory procedures on embryo ploidy and the need to track these outcomes in addition to traditional IVF metrics.
JOURNAL OF OVARIAN RESEARCH
(2022)
Article
Genetics & Heredity
Takashi Shibahara, Yuu Fukasaku, Naoko Hayashi, Nozomi Miyazaki, Hiroaki Kawato, Hiroyuki Minoura
Summary: The study suggests that early ROA after ICSI is effective in rescuing unfertilized oocytes, with a higher normal fertilization rate compared to non-ROA oocytes. However, the blastocyst development rate is lower, while the implantation rate is similar to spontaneously activated oocytes.
JOURNAL OF ASSISTED REPRODUCTION AND GENETICS
(2021)
Article
Obstetrics & Gynecology
Yanhe Liu, Katie Feenan, Vincent Chapple, Peter Roberts, Phillip Matson
Article
Obstetrics & Gynecology
Yanhe Liu, Katie Feenan, Vincent Chapple, Phillip Matson
Editorial Material
Obstetrics & Gynecology
Yanhe Liu, Denny Sakkas, Masoud Afnan, Phillip Matson
Review
Genetics & Heredity
Yanhe Liu, Fang Qi, Phillip Matson, Dean E. Morbeck, Ben W. Mol, Sai Zhao, Masoud Afnan
JOURNAL OF ASSISTED REPRODUCTION AND GENETICS
(2020)
Article
Genetics & Heredity
Kate Watson, Irving Korman, Yanhe Liu, Deirdre Zander-Fox
Summary: Live birth can be achieved in patients with zona production deficiency. Adjustments in ovarian stimulation and subsequent embryo culture strategies may have contributed to the success of the third cycle.
JOURNAL OF ASSISTED REPRODUCTION AND GENETICS
(2021)
Review
Obstetrics & Gynecology
James M. Kemper, Yanhe Liu, Masoud Afnan, Elizabeth R. Hammond, Dean E. Morbeck, Ben W. J. Mol
Summary: Embryo quality is crucial for the success of IVF, with low-grade blastocysts (LGB) not receiving enough attention in existing scoring systems. Studies show that LGB have similar euploidy and pregnancy success rates after implantation, with no adverse effects on pregnancy or perinatal outcomes. Evidence for pregnancy outcomes is lacking for different grades of LGB, with most studies comparing all LGB as one category with optimal blastocysts.
REPRODUCTIVE BIOMEDICINE ONLINE
(2021)
Article
Genetics & Heredity
Lee-Sarose Orevich, Kate Watson, Kee Ong, Irving Korman, Ross Turner, David Shaker, Yanhe Liu
Summary: This study examined the morphometric and morphokinetic features of male and female pronuclei (PN) in human zygotes. The results showed that male and female zygotes had different time intervals and size differences in PN appearance. Male zygotes also had a higher rate of central PN juxtaposition compared to female zygotes. These findings suggest that embryo selection based on these parameters may result in an unbalanced sex ratio in offspring.
JOURNAL OF ASSISTED REPRODUCTION AND GENETICS
(2022)
Editorial Material
Obstetrics & Gynecology
Masoud Afnan, Michael Anis Mihdi Afnan, Yanhe Liu, Julian Savulescu, Abhishek Mishra, Vincent Conitzer, Cynthia Rudin
Summary: The past decade has witnessed a rapid growth of machine learning applications in healthcare, but the premature implementation of these algorithms has led to mixed and sometimes negative outcomes. This paper emphasizes the critical need for "data solidarity" in machine learning for embryo selection, which involves ensuring individual rights, promoting data justice and equity, and utilizing data for the public good.
REPRODUCTIVE BIOMEDICINE ONLINE
(2022)
Article
Obstetrics & Gynecology
Haowen Zou, James M. Kemper, Elizabeth R. Hammond, Fengqin Xu, Gensheng Liu, Lintao Xue, Xiaohong Bai, Hongqing Liao, Songguo Xue, Shuqin Zhao, Lan Xia, Jean Scott, Vincent Chapple, Masoud Afnan, Dean E. Morbeck, Ben W. J. Mol, Yanhe Liu, Rui Wang
Summary: The transfer of single low-grade blastocysts resulted in a reduced live birth rate compared to single good-grade blastocysts, but did not lead to more adverse perinatal outcomes.
HUMAN REPRODUCTION
(2023)
Review
Obstetrics & Gynecology
Kate Bickendorf, Fang Qi, Kelli Peirce, Jay Natalwala, Vincent Chapple, Yanhe Liu
Summary: Spontaneous collapse (SC) of human blastocysts is associated with reduced euploid embryo and pregnancy rates in IVF treatment.
HUMAN REPRODUCTION
(2023)
Review
Obstetrics & Gynecology
James M. Kemper, Yanhe Liu, Masoud Afnan, Ben W. J. Mol, Dean E. Morbeck
Summary: There is a lack of evidence on embryos derived from oocytes without two pronuclei (2PN) or 'normal fertilization'. A significant difference exists between the development potential of oocytes with abnormal pronuclei and those with 2PN. Most recent studies focus on outcomes of blastocysts derived from non-2PN oocytes, suggesting that they have lower blastocyst rates and slightly reduced implantation potential compared to 2PN oocytes.
REPRODUCTIVE BIOMEDICINE ONLINE
(2023)
Review
Obstetrics & Gynecology
Tammy Lee, Jay Natalwala, Vincent Chapple, Yanhe Liu
Summary: With the advancement in computing power and accumulation of embryo image data, artificial intelligence (AI) has been applied in embryo selection in IVF. Machine learning (ML) has the potential to reduce subjectivity and save time, but the lack of interpretability in modern deep learning (DL) techniques has raised concerns. The effectiveness of black-box models lacks confirmation from randomized controlled trials, and recent evidence suggests underperformance compared to interpretable ML models. Interpretable AI is gaining support due to its ethical advantages and technical feasibility. A novel classification system for embryo selection models is proposed, focusing on subjectivity, interpretability, and explainability.
HUMAN REPRODUCTION
(2023)
Meeting Abstract
Obstetrics & Gynecology
B. W. Mol, M. Afnan, J. M. Kemper, F. Xu, G. Liu, L. Xue, X. Bai, H. Liao, S. Xue, S. Zhao, L. Xia, J. Scott, D. Morbeck, Y. Liu
HUMAN REPRODUCTION
(2022)
Article
Obstetrics & Gynecology
Yanh Liu, Kee Ong, Irving Korman, Ross Turner, David Shaker, Deirdre Zander-Fox, Luk Rombauts
Summary: This study found that the timing of assessment on day 5 is a confounding factor in predicting live birth using snapshot blastocyst morphology. A numerical algorithm incorporating day 5 assessment HPI, blastocyst morphology, and maternal age can be developed for live birth prediction.
REPRODUCTIVE BIOMEDICINE ONLINE
(2022)
Article
Obstetrics & Gynecology
Michael Anis Mihdi Afnan, Yanhe Liu, Vincent Conitzer, Cynthia Rudin, Abhishek Mishra, Julian Savulescu, Masoud Afnan
Summary: The potential of using artificial intelligence in the field of in vitro fertilization is significant, but challenges remain in terms of ethical implementation, algorithm interpretability, and clinical accuracy. Current studies show that algorithms can differentiate well between high-quality and poor-quality embryos, but struggle with embryos of similar quality, leading to various epistemic and ethical concerns.
HUMAN REPRODUCTION OPEN
(2021)
Article
Reproductive Biology
Patrycja Kurowska, Ewa Mlyczynska, Julia Wajda, Konrad Krol, Karolina Pich, Patrycja Guzman, Aleksandra Greggio, Oliwia Szkraba, Malgorzata Opydo, Joelle Dupont, Agnieszka Rak
Summary: This study investigated the expression of PNX-14 and GPR173 in ovarian tissue and the effects of PNX-14 on granulosa cell function. The results showed that PNX-14 can influence ovarian follicle growth by promoting granulosa cell proliferation, hormone secretion, and cell cycle progression.
REPRODUCTIVE BIOLOGY
(2024)
Article
Reproductive Biology
Sonia Srivastava, Swarnima Mishra, Gyan Babu, Banalata Mohanty
Summary: This study aimed to investigate the efficacy of PD149163 in preventing/ameliorating LPS-induced inflammation in the female reproductive system of mice. The results showed that PD149163 reduced/prevented the inflammation in the ovary and uterine horn, restored the balance of inflammatory cytokines and oxidative stress, and recovered the levels of reproductive hormones and corticosterone.
REPRODUCTIVE BIOLOGY
(2024)
Article
Reproductive Biology
Chen Zhang, Hongyan Cheng, Xue Ye, Heng Cui, Yi Li, Honglan Zhu, Xiaohong Chang
Summary: Extracellular matrix protein 1 (ECM1) is significantly upregulated in endometriosis tissues. Blocking ECM1 attenuates cell migration and invasion. ECM1 regulates cell migration, invasion, and cytoskeleton reorganization via the RhoC/ROCK1 signaling pathway.
REPRODUCTIVE BIOLOGY
(2024)