Article
Biochemistry & Molecular Biology
Koichiro Yamamoto, Yasuhiro Nakano, Nahoko Iwata, Yoshiaki Soejima, Atsuhito Suyama, Toru Hasegawa, Fumio Otsuka
Summary: The reproductive role of oxytocin (OXT) in ovarian steroidogenesis and its interaction with bone morphogenetic proteins (BMPs) were investigated using rat and human granulosa cells. OXT treatment increased progesterone production in response to FSH and FSK in both cells, while not affecting estradiol production. OXT also enhanced the phosphorylation of SMAD1/5/9 and the transcription of ID1 induced by BMP-15, but not by BMP-6, in human granulosa cells. Overall, OXT accelerates ovarian progesterone synthesis by upregulating BMP-15 activity, thus fine-tuning ovarian steroidogenesis.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2023)
Article
Biochemistry & Molecular Biology
Koichiro Yamamoto, Yasuhiro Nakano, Nahoko Iwata, Yoshiaki Soejima, Atsuhito Suyama, Toru Hasegawa, Fumio Otsuka
Summary: The aim of this study was to investigate the effects of AVP on ovarian steroid production and its relationship with the BMP system in the ovaries. The results showed that AVP treatment increased progesterone synthesis in rat granulosa cells and human granulosa cells, but did not significantly affect estradiol production. AVP also enhanced cAMP synthesis and the expression of progestogenic enzymes in human granulosa cells, and upregulated BMP signaling and receptors. These findings suggest that AVP stimulates progesterone production and fine-tunes its biosynthesis through the activation of the cAMP-PKA pathway and upregulation of BMP signaling.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2023)
Article
Biochemistry & Molecular Biology
Chiaki Kashino, Toru Hasegawa, Yasuhiro Nakano, Nahoko Iwata, Koichiro Yamamoto, Yasuhiko Kamada, Hisashi Masuyama, Fumio Otsuka
Summary: The study revealed that glucocorticoids have differential effects on ovarian steroidogenesis by enhancing the actions of GR and BMP-15 in granulosa cells.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2021)
Article
Biochemistry & Molecular Biology
Magdalena Wytrwal, Malgorzata Sekula-Stryjewska, Agata Pomorska, Ewa Oclon, Ewa Zuba-Surma, Szczepan Zapotoczny, Krzysztof Szczubialka
Summary: This study investigated the covalent binding of recombinant human bone morphogenetic protein-2 and -7 in stem cells and its effects on cell adhesion, migration, and osteogenesis. The presence of both proteins enhanced cell adhesion but limited cell motility, while the presence of single proteins promoted cell migration activity. Additionally, the coexistence of the proteins significantly increased early osteogenic marker expression.
Article
Obstetrics & Gynecology
Samantha Sperduti, Elia Paradiso, Claudia Anzivino, Clara Lazzaretti, Silvia Limoncella, Sara D'Alessandro, Neena Roy, Francesca Reggianini, Tommaso Ferrari, Beatrice Melli, Giovanni Battista La Sala, Alessia Nicoli, Jessica Daolio, Maria Teresa Villani, Simonetta Tagliavini, Tommaso Trenti, Francesco Poti, Reinhild Sandhowe, Chiara Centonze, Monica Lispi, Manuela Simoni, Livio Casarini
Summary: The addition of LH to FSH can recover the cAMP and progesterone production of sub/poor-responder women's ovarian granulosa cells. This study has important implications for personalized ART stimulation protocols.
HUMAN REPRODUCTION
(2023)
Article
Agriculture, Dairy & Animal Science
Leon J. Spicer, Luis F. Schutz, Pauline Y. Aad
Summary: The experimental results indicate that BMP4, GREM, and CTGF have significant regulatory effects on GC function, with a more pronounced effect of BMP4 on large-follicle GCs.
JOURNAL OF ANIMAL SCIENCE
(2021)
Article
Engineering, Biomedical
Sarah A. Schoonraad, Alan A. Jaimes, Arjun J. X. Singh, Kiera J. Croland, Stephanie J. Bryant
Summary: This study investigates the osteogenic effects of tethered BMP-2 and BMP-9 on human MSCs encapsulated in a hydrogel. The results show that tethered BMP-2 and BMP-9 both effectively enhance osteogenesis of MSCs, leading to a mature osteoblast phenotype.
ACTA BIOMATERIALIA
(2023)
Article
Cell Biology
Huakun Huang, Qiuping Lu, Caihong Ye, Mengqi Wei, Chunmei Yang, Lulu Zhang, Yanran Huang, Xiaoji Luo, Jinyong Luo
Summary: TAZ promotes osteogenic differentiation induced by BMP9 through multiple signal pathways.
CELL DEATH DISCOVERY
(2022)
Article
Biochemistry & Molecular Biology
Hyun Seok, Hee-Youl Kim, Dong-Cheol Kang, Jung-Ho Park, Jong Hoon Park
Summary: The study compared the bone regeneration ability of bone scaffolds using particle and block bones with rhBMP-2, showing that rhBMP-2 with block bones resulted in significantly higher bone volume but also led to the formation of adipose tissue and void spaces.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Reproductive Biology
Ya-Qing Tian, Xiao-Li Li, Wan-Jie Wang, Hai-Sheng Hao, Hui-Ying Zou, Yun-Wei Pang, Xue-Ming Zhao, Hua-Bin Zhu, Wei-Hua Du
Summary: This study investigated the expression and function of bone morphogenetic protein 4 (BMP4) gene in bovine cumulus cells (CCs), revealing its role in regulating cell apoptosis, proliferation, and cell cycle. The knockdown of BMP4 gene led to increased cell apoptosis, decreased proliferation, and altered cell cycle in bovine CCs.
Article
Polymer Science
Vijaya Narasimha Gudivada, Chen-Ji Huang, Yueh-Hsia Luo, Guo-Chung Dong
Summary: In this study, novel cyclic peptide analogues were designed to investigate interactions involved in osteogenesis, demonstrating higher binding affinity of peptides with active regions inside the ring. In vitro experiments further confirmed the potential role of these cyclic peptide analogues in osteogenesis.
Article
Engineering, Biomedical
Jonathan Dorogin, Henry B. B. Hochstatter, Samantha O. O. Shepherd, Justin E. E. Svendsen, Morrhyssey A. A. Benz, Andrew C. C. Powers, Karly M. M. Fear, Jakob M. M. Townsend, James S. S. Prell, Parisa Hosseinzadeh, Marian H. H. Hettiaratchi
Summary: By using yeast surface display, unique BMP-2-specific affibodies were identified to modulate the release and activity of BMP-2, offering a promising approach for controlling BMP-2 delivery in clinical applications.
ADVANCED HEALTHCARE MATERIALS
(2023)
Article
Engineering, Biomedical
Sihan Lin, Shi Yin, Junfeng Shi, Guangzheng Yang, Xutao Wen, Wenjie Zhang, Mingliang Zhou, Xinquan Jiang
Summary: In this study, the dose of bone morphogenetic protein-2 (BMP-2) was reduced and its safety and efficacy were improved through the regulation of magnesium ion (Mg2+). Magnesium ion enhanced cellular bioenergetic levels and the osteoinductivity of BMP-2. A co-delivery system, fabricated using microfluidic and 3D printing technologies, was able to rapidly and robustly induce bone regeneration.
BIOACTIVE MATERIALS
(2022)
Review
Medicine, Research & Experimental
Ting Liu, Yifei Huang, Hui Lin
Summary: This review article discusses the regulation and mechanism of aromatase activity in ovarian granulosa cells, as well as the impact of aromatase regulation on estrogen secretion. It highlights the influence of endocrine-disrupting substances and other factors on aromatase expression, which can lead to an imbalance in estrogen secretion by target tissues.
INTERNATIONAL JOURNAL OF MOLECULAR MEDICINE
(2021)
Article
Biotechnology & Applied Microbiology
Gaoli Xu, Chenxi Shen, Haiyan Lin, Jian Zhou, Ting Wang, Ben Wan, Munerah Binshabaib, Tymour Forouzanfar, Guochao Xu, Nawal Alharbi, Gang Wu
Summary: This study synthesized a novel biomimetically-precipitated nanocrystalline calcium phosphate (BpNcCaP) with internally incorporated bone morphogenetic protein-2 (BpNcCaP + BMP-2), demonstrating its potential application in repairing large-volume bone defects.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2022)