Article
Biochemistry & Molecular Biology
Silvana G. Cooper, Lucas A. C. Souza, Caleb J. Worker, Ariana Julia B. Gayban, Sophie Buller, Ryosuke Satou, Yumei Feng Earley
Summary: The brain renin-angiotensin system plays important roles in blood pressure and cardiovascular regulation. This study investigates the role of brain prorenin in the development of salt-sensitive hypertension. It is found that renin-a mRNA is expressed in several regions of the brain, including the subfornical organ, the paraventricular nucleus of the hypothalamus, and the brainstem. Ablation of renin-a in the subfornical organ attenuates hypertension and improves autonomic function without affecting fluid or sodium intake. The findings demonstrate the importance of endogenous renin-a in the pathogenesis of salt-sensitive hypertension.
Review
Nutrition & Dietetics
Jyothi Dhuguru, Ryan W. W. Dellinger, Marie E. E. Migaud
Summary: Dietary vitamin B3 components have been found to increase NAD(+) levels, which decline with age and disease. Supplementation with NAD(+) biosynthetic intermediates, such as NR and NMN, can effectively raise NAD(+) levels. In addition, inhibiting NAD(+)-consuming enzymes or activating biosynthetic pathways is also a viable approach to increase NAD(+) and NADH. However, the pharmacological significance of NAD(H) catabolites is often overlooked, despite some of these metabolites serving as biomarkers in physiological disorders.
Article
Chemistry, Applied
Jingyu Wang, Elif Erdem, John M. Woodley
Summary: Biocatalytic oxidation holds promise for the selective synthesis of active pharmaceutical intermediates. Bubbling air or oxygen is an effective method to enhance oxygen transfer, but it leads to enzyme deactivation. This study investigates the stability of NAD-(P)H oxidase (NOX) at different gas-liquid interfaces and provides insights for improving biocatalyst performance.
ORGANIC PROCESS RESEARCH & DEVELOPMENT
(2023)
Review
Chemistry, Multidisciplinary
Andreas S. Bommarius
Summary: While many oxidases are active and selective, they often lack stability for chemical synthesis or large-scale processing due to various stresses, such as loss of flavin co-enzyme, overoxidation in the presence of reactive oxygen species, or instability in the presence of air/water interfaces if the co-substrate oxygen is supplied by sparging.
CURRENT OPINION IN GREEN AND SUSTAINABLE CHEMISTRY
(2023)
Article
Chemistry, Physical
Jingyu Wang, John M. Woodley
Summary: This paper investigates the regeneration of important cofactors NAD(P)H and NAD(P) in biocatalytic synthesis for ensuring the economic sustainability of the process. The kinetic stability of a water-forming NAD(P)H oxidase (NOX) for in-situ cofactor regeneration in a bubble column is studied. The results show that NOX exhibits two-stage and single-stage deactivation kinetics, with aggregation and unfolding at the gas-liquid interface being the main cause of deactivation.
Article
Chemistry, Multidisciplinary
Sooyoung Lim, Hee-wang Yoo, Sharad Sarak, Byung-gee Kim, Hyungdon Yun
Summary: The study optimized the enzymatic production of dodecanedioic acid from fatty acids, identified a suitable enzyme for the process, and achieved improved productivity through fusion protein method.
JOURNAL OF INDUSTRIAL AND ENGINEERING CHEMISTRY
(2021)
Article
Chemistry, Applied
Marc Biermann, Martin Schuermann, Thomas Schmitges, Andreas Vogel, Jan Brummund
Summary: A biotransformation method to produce (R)-Undecavertol has been developed and successfully demonstrated at the pilot scale with high productivity. By using a highly selective alcohol dehydrogenase and NAD(P)H oxidase, racemic resolution of the substrate is achieved, and oxygen limitation is prevented by the supply of pure oxygen.
ORGANIC PROCESS RESEARCH & DEVELOPMENT
(2022)
Article
Biochemistry & Molecular Biology
Polina Ermakova, Alena Kashirina, Irina Kornilova, Aleksandra Bogomolova, Darya Myalik, Nasipbek Naraliev, Denis Kuchin, Liya Lugovaya, Elena Zagaynova, Vladimir Zagainov, Aleksandra Kashina
Summary: FLIM is a potential tool for diagnosing islet cell recovery after therapy. This study identified key FLIM parameters associated with pancreatic pathologies and confirmed their diagnostic value for islet cells.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Chemistry, Multidisciplinary
Maodi Wang, Huicong Dai, Qihua Yang
Summary: In this study, a metal nanoparticle and metal complex integrated core-shell nanoreactor was prepared for H2-driven NAD(P)H regeneration. The integrated catalyst showed enhanced activity and selectivity in NAD(P)H regeneration due to the spillover effect of activated H species. The size-sieving effect of the porous organic polymer enabled the coupling of the core-shell nanoreactor with aldehyde ketone reductase for chemoenzymatic reduction.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2023)
Article
Environmental Sciences
Michela Novelli, Pascale Beffy, Matilde Masini, Chiara Vantaggiato, Luisa Martino, Lorella Marselli, Piero Marchetti, Vincenzo De Tata
Summary: Exposure to environmental pollutant TCDD affects pancreatic islets, especially beta cells, leading to cell death, ultrastructural alterations, decreased insulin secretion, and changes in gene expression. TCDD exposure significantly reduces cell survival and insulin secretion, causes ultrastructural changes in beta cells, and alters gene expression profiles in isolated islets.
Review
Plant Sciences
Ian Max Moller, Allan G. Rasmusson, Olivier Van Aken
Summary: The study of plant mitochondria began in the 1950s, focusing on establishing basic properties and unique characteristics compared to mammalian mitochondria. With advancements in molecular biology and genomics, further unique features were discovered, including the large size of plant mtDNA, unique mRNA maturation process, and a large proteome with many unique proteins.
Article
Chemistry, Analytical
Jie Zhang, Zengchen Liu, Fengshou Tian, Yahong Chen
Summary: A fluorescent compound derived from coumarin and hemicyanine was synthesized and characterized in this paper, showing higher ratiometric fluorescence response activity toward NAD(P)H compared to other commonly coexisting compounds in the cell microenvironment. The fluorescence identification mechanism was deduced to be a redox reaction between the sensor and NAD(P)H. This ratiometric fluorescent probe provides a theoretical basis for sensing NAD(P)H in vitro and in vivo, and was used to build a sensitive detection platform of NAD(P)H-dependent enzyme activity based on the fluorescence method.
ANALYTICAL METHODS
(2021)
Article
Chemistry, Multidisciplinary
Kaiqing Ma, He Yang, Xingkang Wu, Fangjun Huo, Fangqin Cheng, Caixia Yin
Summary: A new liposoluble cationic probe, KC8, was developed to detect NAD(P)H and monitor the abnormality of p53 in colorectal cancer cells in real time.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2023)
Article
Plant Sciences
Xiaojie Cheng, Ulrike Frank, Feng Zhao, Julia Ruiz Capella, J. Barbro Winkler, Jorg-Peter Schnitzler, Andrea Ghirardo, Marko Bertic, Nicole Estrella, Jorg Durner, Karin Pritsch
Summary: Ongoing global warming promotes the spread of thermo-tolerant plant species, including plants with a high allergenic risk like common ragweed. To investigate the effects of temperature increase on plant traits and biochemical processes related to allergenicity, a phytotron chamber experiment was conducted. The results showed that elevated temperature increased plant height, size of male inflorescences, duration of pollen release, and delayed pollen release, while elevated NO2 concentration induced pollen release and enhanced allergenicity. Combined treatment of elevated temperature and NO2 resulted in the highest allergenicity.
ENVIRONMENTAL AND EXPERIMENTAL BOTANY
(2023)
Article
Microbiology
Alejandro Arce-Rodriguez, Debbie Pankratz, Matthias Preusse, Pablo Nikel, Susanne Haeussler
Summary: This study artificially manipulated the redox balance of Pseudomonas aeruginosa and found that increasing intracellular NADH concentrations led to higher antibiotic resistance but also increased production of ROS, enhancing antimicrobial killing. This suggests that disrupting cellular redox homeostasis could enhance antibiotic killing by sensitizing pathogens to currently available antibiotics.
Article
Pharmacology & Pharmacy
Eloisa Aparecida Vilas-Boas, Noemie Karabacz, Gabriela Nunes Marsiglio-Librais, Maira Melo Rezende Valle, Lisa Nalbach, Emmanuel Ampofo, Bruce Morgan, Angelo Rafael Carpinelli, Leticia Prates Roma
PHARMACOLOGICAL REPORTS
(2020)
Article
Nutrition & Dietetics
Ana Claudia Munhoz, Eloisa Aparecida Vilas-Boas, Ana Carolina Panveloski-Costa, Jaqueline Santos Moreira Leite, Camila Ferraz Lucena, Patricia Riva, Henriette Emilio, Angelo R. Carpinelli
Article
Biochemistry & Molecular Biology
Debora L. Quintilhano, Daniele R. Miksza, Giuliana R. Biazi, Isabele G. Frasson-Uemura, Maria Fernanda R. Graciano, Tania L. Mazzuco, Angelo R. Carpinelli, Helenir M. de Souza, Gisele L. Bertolini
Summary: Lixisenatide has shown effectiveness in increasing insulin secretion and reducing insulin resistance in T2DM, but it does not have the same benefits for cancer patients with hypoinsulinemia and insulin resistance, and may worsen weight loss. Therefore, its use in T2DM patients with cancer should be avoided.
CELL BIOCHEMISTRY AND FUNCTION
(2021)
Review
Nutrition & Dietetics
Ailton Santos Sena-Junior, Felipe Jose Aidar, Ana Mara de Oliveira e Silva, Charles dos Santos Estevam, Carla Roberta de Oliveira Carvalho, Fabio Bessa Lima, Jymmys Lopes dos Santos, Anderson Carlos Marcal
Summary: Curcumin, a plant extract, shows potential in attenuating hyperglycemia caused by diabetes mellitus. Supplementation of curcumin may have positive effects on antioxidant and hypoglycemic potential in Type 1 and Type 2 diabetes. The combination of curcumin and physical exercise could be beneficial in improving the quality of life and extending survival for diabetic patients.
Article
Biochemistry & Molecular Biology
Andre Guaraci DeVito-Moraes, Victor Di Donato Marques, Luciana Chagas Caperuto, Flavia Kazue Ibuki, Fernando Neves Nogueira, Carlos Eduardo Francci, Carla Roberta de Oliveira Carvalho
Summary: The parotid gland, as the largest salivary gland, plays a significant role in saliva production. Research has shown that insulin has acute effects on intracellular pathways in the parotid glands. These findings are important for understanding salivary gland issues in diabetic individuals.
CELL BIOCHEMISTRY AND BIOPHYSICS
(2022)
Article
Biochemistry & Molecular Biology
Eloisa A. Vilas-Boas, Christopher Carlein, Lisa Nalbach, Davidson C. Almeida, Emmanuel Ampofo, Angelo R. Carpinelli, Leticia P. Roma, Fernanda Ortis
Summary: In the development of type 1 diabetes, proinflammatory cytokines released by immune cells lead to increased reactive oxygen species production in beta-cells. NOX2 may be a potential target for therapies against early beta-cell dysfunction.
Review
Genetics & Heredity
Tais Menezes do Moinho, Sandro Leao Matos, Carla R. O. Carvalho
Summary: Non-alcoholic fatty liver disease (NAFLD) is the most common liver disease in the world and is associated with obesity. Currently, there is no official treatment for NAFLD, but there are synthetic drugs and herbal products being studied for potential treatment.
JOURNAL OF MOLECULAR MEDICINE-JMM
(2022)
Review
Cell Biology
Eloisa Aparecida Vilas-Boas, Davidson Correa Almeida, Leticia Prates Roma, Fernanda Ortis, Angelo Rafael Carpinelli
Summary: A high caloric intake rich in saturated fats contributes to obesity development, which is the main risk factor for type 2 diabetes. Lipotoxicity induced by fatty acids leads to ER stress and oxidative stress in pancreatic beta-cells, causing dysfunction and death of the cells. The main source of oxidative stress in beta-cells seems to be the NADPH oxidase enzyme, which plays a role in insulin secretion and beta-cell demise in both T1 and T2D.
Article
Biochemistry & Molecular Biology
Maira M. R. Valle, Eloisa Aparecida Vilas-Boas, Camila F. Lucena, Simone A. Teixeira, Marcelo N. Muscara, Angelo R. Carpinelli
Summary: This study evaluated the antioxidant effects of metformin on insulin-producing cells, and found that it disrupted insulin secretion and β-cell metabolism in a concentration-dependent manner. At high concentrations, metformin induced cell death and increased ROS production, while reducing GSH content. However, it did not alter the activity of antioxidant enzymes and transcription factor Nrf2.
JOURNAL OF BIOCHEMICAL AND MOLECULAR TOXICOLOGY
(2022)
Article
Endocrinology & Metabolism
Katherine Veras, Camila Ferraz Lucena, Julia Goedcke, Fabiana S. Evangelista, Angelo Carpinelli, Carla Roberta de Oliveira Carvalho
Summary: This study evaluated the effects of moderated aerobic treadmill exercise training on pancreatic islet cell viability and function in mice consuming a high-fat and sucrose diet. The results showed that exercise training can slow down and prevent adverse metabolic effects associated with consuming a westernized diet.
FRONTIERS IN ENDOCRINOLOGY
(2022)
Article
Medicine, Research & Experimental
Gabriela V. Moreira, Layanne C. C. Araujo, Gilson M. Murata, Sandro L. Matos, Carla R. O. Carvalho
Summary: Nonalcoholic fatty liver disease (NAFLD), often associated with obesity, is a common liver disease worldwide. The gut-liver axis is considered the main pathway for NAFLD development. An exploratory study showed that a kombucha probiotic has beneficial effects on reducing metabolic abnormalities caused by diet-induced obesity.
BIOMEDICINE & PHARMACOTHERAPY
(2022)
Article
Multidisciplinary Sciences
Natalia Tobar, Guilherme Z. Rocha, Andrey Santos, Dioze Guadagnini, Heloisa B. Assalin, Juliana A. Camargo, Any E. S. S. Goncalves, Flavia R. Pallis, Alexandre G. Oliveira, Silvana A. Rocco, Raphael M. Neto, Irene Layane de Sousa, Marcos R. Alborghetti, Mauricio L. Sforca, Patricia B. Rodrigues, Raissa G. Ludwig, Emerielle C. Vanzela, Sergio Q. Brunetto, Patricia A. Boer, Jose A. R. Gontijo, Bruno Geloneze, Carla R. O. Carvalho, Patricia O. Prada, Franco Folli, Rui Curi, Marcelo A. Mori, Marco A. R. Vinolo, Celso D. Ramos, Kleber G. Franchini, Claudio F. Tormena, Mario J. A. Saad
Summary: Metformin primarily acts on the gut, increasing basolateral intestinal glucose uptake (BIGU) and influencing hepatic glucose production (HGP). In hyperglycemia, it modulates lactate and acetate production in the gut, reducing HGP. In normoglycemia, its mechanism of action is different.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2023)
Article
Biochemistry & Molecular Biology
Felipe N. Camargo, Sandro L. Matos, Layanne C. C. Araujo, Carla R. O. Carvalho, Andressa G. Amaral, Joao Paulo Camporez
Summary: One consequence of Western lifestyle and high-fat diet is non-alcoholic fatty liver disease (NAFLD) and its aggressive form, non-alcoholic steatohepatitis (NASH). NASH can progress to cirrhosis and hepatocellular carcinoma (HCC) and is becoming the leading cause of end-stage liver disease or liver transplantation. This study aimed to characterize a fast rodent model with all characteristic features of NASH and found that the ApoE KO mouse fed with Western diet is a great model for NASH research.
CURRENT ISSUES IN MOLECULAR BIOLOGY
(2022)
Article
Physiology
Joice Naiara Bertaglia Pereira, Gilson Masahiro Murata, Fabio Takeo Sato, Aline Rosa Marosti, Carla Roberta de Oliveira Carvalho, Rui Curi
Summary: GK rats exhibit small intestine remodeling associated with marked alterations in morphology, local inflammation, and reduced intestinal transit.
PHYSIOLOGICAL REPORTS
(2021)
Article
Biochemistry & Molecular Biology
Gabriela Nunes Marsiglio-Librais, Eloisa Aparecida Vilas-Boas, Christopher Carlein, Markus Daniel Alexander Hoffmann, Leticia Prates Roma, Angelo Rafael Carpinelli
