Review
Biochemistry & Molecular Biology
Tianxin Gao, Pei Wang, Teng Gong, Ying Zhou, Ancong Wang, Xiaoying Tang, Xiaolei Song, Yingwei Fan
Summary: The use of molecular imaging technologies in brain imaging is not only important for disease diagnosis and treatment, but also for in-depth study of brain functions. Reporter gene technology based on optical imaging has made breakthroughs in molecular-level brain function studies. The applications of magnetic resonance imaging (MRI), single-photon emission computed tomography (SPECT), and positron emission tomography (PET) reporter gene technologies in brain imaging are discussed, with a focus on cell therapies and gene therapies. These methods have shown potential for preclinical and clinical research, providing deeper imaging depths and wider imaging ranges.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Medicine, Research & Experimental
Vidyani Suryadevara, Mohammad Javad Hajipour, Lisa C. Adams, Nour Mary Aissaoui, Ali Rashidi, Louise Kiru, Ashok J. Theruvath, Ching-Hsin Huang, Masahiro Maruyama, Masanori Tsubosaka, Jennifer K. Lyons, Wei (Emma) Wu, Raheleh Roudi, Stuart B. Goodman, Heike E. Daldrup-Link
Summary: This study developed an efficient labeling method for MSCs using MegaPro nanoparticles, which showed better effectiveness in tracking MSCs compared to ferumoxytol nanoparticles. MegaPro-labeled MSCs demonstrated enhanced MRI tracking without affecting cell viability or differentiation.
Review
Oncology
Laura M. Lechermann, Doreen Lau, Bala Attili, Luigi Aloj, Ferdia A. Gallagher
Summary: This review discusses the potential of using non-invasive imaging methods for tracking therapeutic cells, particularly in oncology. The focus is on the role of radiopharmaceuticals for non-invasive detection and tracking of therapeutic cells using positron emission tomography (PET). Evaluation of PET imaging methods in preclinical cancer models and overcoming translational challenges are also examined to improve the accuracy and efficacy of cell therapies.
Article
Radiology, Nuclear Medicine & Medical Imaging
Nourhan Shalaby, John Kelly, Francisco Martinez, Mathew Fox, Qi Qi, Jonathan Thiessen, Justin Hicks, Timothy J. Scholl, John A. Ronald
Summary: The study developed a dual MRI/PET reporter gene system for noninvasive tracking of engineered cells, combining the high resolution of MRI and sensitivity of PET, offering a new approach to cell tracking and potentially broader applications within the healthcare system in the future.
MOLECULAR IMAGING AND BIOLOGY
(2022)
Article
Biotechnology & Applied Microbiology
Federica Pisaneschi, Seth T. Gammon, Vincenzo Paolillo, Sarah A. Qureshy, David Piwnica-Worms
Summary: This study introduces a novel redox-tuned radiopharmaceutical called [F-18]4FN, which selectively binds proteins and cells oxidized by products of human MPO and H2O2, and can be detected using PET/CT technology. It shows promise as a robust reporter of activated innate immunity and has been successfully used to detect inflammation in mouse models.
NATURE BIOTECHNOLOGY
(2022)
Review
Cell & Tissue Engineering
Xiaoshuang Zhang, Tong Lei, Hongwu Du
Summary: Stem cell therapy has shown great efficacy in many diseases, but the treatment mechanism is unclear, making it important to track transplanted stem cells to reveal the treatment mechanism. Cell penetrating peptides (CPPs) have potential in stem cell tracking due to their high efficacy and safety properties.
STEM CELL RESEARCH & THERAPY
(2021)
Review
Chemistry, Multidisciplinary
Chavee Laomeephol, Sudartip Areecheewakul, Supannikar Tawinwung, Koramit Suppipat, Preedakorn Chunhacha, Nuno M. Neves, Jittima Amie Luckanagul
Summary: Chimeric antigen receptor (CAR) T cell therapy has shown remarkable efficacy in cancer treatment, but its complex manufacturing process limits its application. In vivo generation of CAR T cells provides an alternative immunotherapy. Hyaluronic acid (HA) is a natural biopolymer that can be used for gene delivery due to its inherent properties. This review discusses the potential of HA in delivering CAR constructs and provides insights into rational design of HA-based systems for efficient CAR delivery to circulating T cells.
Review
Chemistry, Multidisciplinary
Peter J. Gawne, Francis Man, Philip J. Blower, Rafael T. M. de Rosales
Summary: The arrival of cell-based therapies has revolutionized medicine. Radionuclide imaging, such as SPECT or PET, is a highly sensitive and quantitative imaging technique that has advantages for cell tracking. Direct cell radiolabeling, using a variety of chemical approaches, is a simple and reliable method for labeling cells without genetic modification.
Review
Neurosciences
Sridhar Goud Nerella, Michael Michaelides, Takafumi Minamimoto, Robert B. Innis, Victor W. Pike, Mark A. G. Eldridge
Summary: Positron emission tomography (PET) can be used as a noninvasive method to monitor and quantify protein expression in the brain over time. It is useful for tracking changes in biomarkers of mental health disorders and evaluating therapeutic interventions like stem cell and molecular genetic therapies. The effectiveness of PET monitoring relies on the availability of radiotracers that have good central nervous system penetration and high selectivity for the target protein.
TRENDS IN NEUROSCIENCES
(2023)
Article
Chemistry, Analytical
Dongxia Chen, Yaying Lin, Ao Li, Xiangjie Luo, Chaoyong Yang, Jinhao Gao, Hongyu Lin
Summary: This study presents a strategy called bio-orthogonal metabolic fluorine labeling for selective cellular F-19 labeling, enabling high specific imaging of tumor cells in vivo. The strategy involves the display of azido groups on the cell surface and subsequent bio-orthogonal ligation to incorporate F-19-containing moieties on the cancer cell surface. The feasibility of this method was validated on the cellular level and further demonstrated for in vivo deep-tissue visualization of cancer cells using F-19 MRI.
ANALYTICAL CHEMISTRY
(2022)
Article
Chemistry, Multidisciplinary
Nourhan Shalaby, John J. Kelly, Olivia C. Sehl, Julia J. Gevaert, Matthew S. Fox, Qi Qi, Paula J. Foster, Jonathan D. Thiessen, Justin W. Hicks, Timothy J. Scholl, John A. Ronald
Summary: Stem cell-based therapies have shown great potential in treating debilitating diseases, and tracking the location and viability of stem cells post administration is crucial. This study engineered mesenchymal stem cells to express a PET reporter gene and labeled them with SPIOs for detection with MPI. Imaging was performed over a 30-day period, showing sensitive early detection with MPI and longer-term viability measurements with PET. This study demonstrates the complementary benefits of MPI and PET for stem cell tracking.
Article
Chemistry, Multidisciplinary
Xinling Li, Xiaochun Yang, Zhijian Li, Xiaobin Zheng, Yong-Jian Peng, Wenjie Lin, Ling Zhou, Dehai Cao, Minyi Situ, Qingqiang Tu, Huiqiang Huang, Wei Fan, Guokai Feng, Xiaofei Zhang
Summary: Cell therapies have achieved cures for hematopoietic disorders, neurodegenerative diseases, and cancer, but not all patients can benefit from them. The clinical methods for monitoring the therapeutic functions of transferred cells over time are limited, resulting in uncertain prognosis. Positron emission tomography (PET) cell tracking provides comprehensive information on the proliferation status and distribution of therapeutic cells. This study developed the first SNAP-tagged PET radiotracer, which can selectively target SNAP-tagged cells for in vivo cell tracking, with promising results in vitro and in vivo.
Article
Multidisciplinary Sciences
Daisuke Arai, Yoichi Nakao
Summary: In this study, an optimized HDR-mediated knock-in method for mouse embryonic stem cells was developed, which significantly improved efficiency and minimized random integrations caused by NHEJ/MMEJ. The BiPoD method achieved efficient plasmid donor insertion and increased biallelic knock-in efficiency, providing a rapid and easy way to establish biallelic knock-in mESC lines.
SCIENTIFIC REPORTS
(2021)
Article
Cell Biology
Sebastian Lungu-Mitea, Yuxin Han, Johan Lundqvist
Summary: The paradigm shift in toxicology in the twenty-first century requires the development of alternative in vitro test systems, especially in the field of ecotoxicology. Transient reporter gene assays could serve as a quick, economical, and reliable bridging technology, but potential pitfalls influenced by reporter vector geometry should be taken into account. In this study, an AhR-responsive transient reporter gene assay was developed in a permanent zebrafish hepatocyte cell line, and a novel normalization vector was designed to counteract the squelching effect caused by viral, constitutive promoters in reporter gene assay cassettes.
CELL BIOLOGY AND TOXICOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Tianqi Liu, Zhenlin Li, Xiaodong Li, Ruiting Zhao, Xinhua Wei, Zixin Wang, Sherman Xuegang Xin
Summary: Exosomes play a significant role in promoting tumor progression, and magnetic resonance imaging (MRI) is an ideal technique to visualize their dynamics. A novel self-label method was proposed for imaging labeled exosomes of tumor cells in vivo through MRI.
BIOCHIMICA ET BIOPHYSICA ACTA-GENERAL SUBJECTS
(2022)
Article
Cardiac & Cardiovascular Systems
Robert Zilberszac, Patrizio Lancellotti, Dan Gilon, Harald Gabriel, Michael Schemper, Gerald Maurer, Massimo Massetti, Raphael Rosenhek
EUROPEAN HEART JOURNAL-CARDIOVASCULAR IMAGING
(2017)
Article
Cardiac & Cardiovascular Systems
Maximilian Tscharre, Bernhard Jaeger, Serdar Farhan, Guenter Christ, Wolfgang Schreiber, Franz Weidinger, Thomas Stefenelli, Georg Delle-Karth, Alfred Kaff, Gerald Maurer, Kurt Huber
INTERNATIONAL JOURNAL OF CARDIOLOGY
(2017)
Article
Cardiac & Cardiovascular Systems
Edita Piackova, Bernhard Jaeger, Serdar Farhan, Guenter Christ, Wolfgang Schreiber, Franz Weidinger, Thomas Stefenelli, Georg Delle-Karth, Alfred Kaff, Gerald Maurer, Kurt Huber
INTERNATIONAL JOURNAL OF CARDIOLOGY
(2017)
Article
Cardiac & Cardiovascular Systems
Matthias Schneider, Anna Maria Pistritto, Christian Gerges, Mario Gerges, Christina Binder, Irene Lang, Gerald Maurer, Thomas Binder, Georg Goliasch
INTERNATIONAL JOURNAL OF CARDIOVASCULAR IMAGING
(2018)
Article
Medicine, Research & Experimental
Mariann Gyongyosi, Noemi Pavo, Dominika Lukovic, Katrin Zlabinger, Andreas Spannbauer, Denise Traxler, Georg Goliasch, Ljubica Mandic, Jutta Bergler-Klein, Alfred Gugerell, Andras Jakab, Zsuzsanna Szankai, Levente Toth, Rita Garamvolgyi, Gerald Maurer, Frederic Jaisser, Faiez Zannad, Thomas Thum, Sandor Batkai, Johannes Winkler
JOURNAL OF TRANSLATIONAL MEDICINE
(2017)
Article
Pharmacology & Pharmacy
Konstantin A. Krychtiuk, Christoph Kaun, Philipp J. Hohensinner, Stefan Stojkovic, Jacqueline Seigner, Stefan P. Kastl, Andreas Zuckermann, Wolfgang Eppel, Sabine Rauscher, Rainer de Martin, Gerald Maurer, Kurt Huber, Johann Wojta, Walter S. Speidl
VASCULAR PHARMACOLOGY
(2017)
Article
Medicine, General & Internal
Paul Michael Haller, Bernhard Jaeger, Serdar Farhan, Guenter Christ, Wolfgang Schreiber, Franz Weidinger, Thomas Stefenelli, Georg Delle-Karth, Alfred Kaff, Gerald Maurer, Kurt Huber
WIENER KLINISCHE WOCHENSCHRIFT
(2018)
Article
Cardiac & Cardiovascular Systems
Robert Zilberszac, Harald Gabriel, Michael Schemper, Guenther Laufer, Gerald Maurer, Raphael Rosenhek
JACC-CARDIOVASCULAR IMAGING
(2017)
Article
Oncology
Noemi Pavo, Dominika Lukovic, Katrin Zlabinger, David Lorant, Georg Goliasch, Johannes Winkler, Dietmar Pils, Katharina Auer, Hendrik Jan Ankersmit, Zoltan Giricz, Marta Sarkozy, Andras Jakab, Rita Garamvolgyi, Maximilian Y. Emmert, Simon P. Hoerstrup, Derek J. Hausenloy, Peter Ferdinandy, Gerald Maurer, Mariann Gyoengyoesi
Article
Multidisciplinary Sciences
Georg Goliasch, Guenther Silbernagel, Marcus E. Kleber, Tanja B. Grammer, Stefan Pilz, Andreas Tomaschitz, Philipp E. Bartko, Gerald Maurer, Wolfgang Koenig, Alexander Niessner, Winfried Maerz
SCIENTIFIC REPORTS
(2017)
Editorial Material
Cardiac & Cardiovascular Systems
Julien Magne, Ehud Schwammenthal, Gerald Maurer, Thor Edvardsen, Bogdan A. Popescu
EUROPEAN HEART JOURNAL-CARDIOVASCULAR IMAGING
(2018)
Article
Cardiac & Cardiovascular Systems
Daniel Lindholm, Emil Hagstrom, Stefan K. James, Richard C. Becker, Christopher P. Cannon, Anders Himmelmann, Hugo A. Katus, Gerald Maurer, Jose Luis Lopez-Sendon, Philippe Gabriel Steg, Robert F. Storey, Agneta Siegbahn, Lars Wallentin
JOURNAL OF THE AMERICAN HEART ASSOCIATION
(2017)
Article
Multidisciplinary Sciences
Konstantin A. Krychtiuk, Maria C. Honeder, Max Lenz, Gerald Maurer, Johann Wojta, Gottfried Heinz, Kurt Huber, Walter S. Speidl
Article
Multidisciplinary Sciences
Noemi Pavo, Dominika Lukovic, Katrin Zlabinger, Abelina Zimba, David Lorant, Georg Goliasch, Johannes Winkler, Dietmar Pils, Katharina Auer, Hendrik Jan Ankersmit, Zoltan Giricz, Tamas Baranyai, Marta Sarkozy, Andras Jakab, Rita Garamvolgyi, Maximilian Y. Emmert, Simon P. Hoerstrup, Derek J. Hausenloy, Peter Ferdinandy, Gerald Maurer, Mariann Gyongyosi
SCIENTIFIC REPORTS
(2017)
Article
Cardiac & Cardiovascular Systems
Bernhard Jaeger, Serdar Farhan, Miklos Rohla, Guenter Christ, Andrea Podczeck-Schweighofer, Wolfgang Schreiber, Anton N. Laggner, Franz Weidinger, Thomas Stefenelli, Georg Delle-Karth, Alfred Kaff, Gerald Maurer, Kurt Huber
EUROPEAN HEART JOURNAL-ACUTE CARDIOVASCULAR CARE
(2017)