期刊
REACTIVE & FUNCTIONAL POLYMERS
卷 76, 期 -, 页码 26-31出版社
ELSEVIER
DOI: 10.1016/j.reactfunctpolym.2014.01.006
关键词
Collagen peptide; Microbial transglutaminase; Antioxidant activity; L929 fibroblast; Cell viability
资金
- National Natural Science Foundation of China [51173143, 51273156]
- Special Funds Project of Major New Products of Hubei Province [20132h0040]
- University-industry Cooperation Projects of The Ministry of Education of Guangdong province [2012B091100437]
- innovation fund project of the Ministry of Science and Technology of Small and Medium-sized Enterprises [11C26214202642, 11C26214212743]
- Zhuhai Science and Technology Plan Projects [2011B050102003]
- Wuhan Science and Technology Development [201060623262]
Collagen peptide grafted carboxymethylated chitosan was synthesized by using microbial transglutaminase as bio-catalyst. The catalyzed reaction exhibited high efficiency, selectivity, environmental friendliness. Collagen peptide grafted carboxymethylated chitosan was characterized by Fourier transform infrared spectroscopy. The process conditions were optimized including the reaction time, the reaction temperature, the molar ratio of collagen peptide to carboxymethylated chitosan and the pH value. The different molecular weight, concentration and degree of substitution of collagen peptide-carboxymethylated chitosan had crucial effects on the hydrogen peroxide scavenging activity of collagen peptide-carboxymethylated chitosan. The methyl thiazolyl tetrazolium assay showed that at a suitable concentration collagen peptide-carboxymethylated chitosan with different degree of substitution value could promote L929 mouse fibroblasts effectively. Therefore, the results suggest that collagen peptide-carboxymethylated chitosan could be potential wound dressings for clinical applications. (C) 2014 Elsevier Ltd. All rights reserved.
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