Article
Cell & Tissue Engineering
Suzanne M. Watt, Hans-Jorg Buhring, Paul J. Simmons, Andrew W. C. Zannettino
Summary: Accurately defining hierarchical relationships between human stem cells and their progeny is crucial for new cellular therapies. The biomarker CD164 has been identified as important for enriching human stem cells, but characteristics of CD164 monoclonal antibodies need further study for effective stem cell identification and purification.
NPJ REGENERATIVE MEDICINE
(2021)
Article
Gastroenterology & Hepatology
Nevi Lorenzo, Di Matteo Sabina, Carpino Guido, Zizzari Ilaria Grazia, Safarikia Samira, Ambmsino Valeria, Costantini Daniele, Overi Diletta, Giancotti Antonella, Monti Marco, Bosco Daniela, De Peppo Valerio, Oddi Andrea, De Rose Agostino Maria, Melandro Fabio, Bragazzi Maria Consiglia, Faccioli Jessica, Massironi Sara, Grazi Gian Luca, Panici Pierluigi Benedetti, Berloco Paquale Bartomeo, Giuliante Felice, Cardinale Vincenzo, Invernizzi Pietro, Caretti Giuseppina, Gaudio Eugenio, Alvaro Domenico
Summary: The study evaluated the expression and function of DCLK1 in intrahepatic and perihilar cholangiocarcinoma cells. DCLK1 was identified as a specific CSC marker and its inhibitor showed anti-neoplastic effects. In addition, DCLK1 was found to be highly expressed in tumor tissues and may serve as a serum biomarker for early diagnosis of cholangiocarcinoma.
Article
Veterinary Sciences
Taro Ayabe, Masaharu Hisasue, Yoko Yamada, Suguru Nitta, Kaoruko Kikuchi, Sakurako Neo, Yuki Matsumoto, Ryo Horie, Kosuke Kawamoto
Summary: This study characterizes the haematopoietic ability and gene expression profiles of canine bone marrow HSPCs in healthy dogs. The CD34+/CD45(dim) cells exhibited higher haematopoietic colony formation ability and gene expression profiles similar to human and mouse HSPCs. The findings suggest that these cells could be used for studying haematopoietic stem cells in dogs.
FRONTIERS IN VETERINARY SCIENCE
(2022)
Article
Cell & Tissue Engineering
Silvia Preciado, Ma Salome Sirerol-Piquer, Sandra Muntion, Lika Osugui, Gerardo J. Marti-Chillon, Almudena Navarro-Bailon, Pilar Sepulveda, Fermin Sanchez-Guijo
Summary: Co-transplantation of human CD34(+) cells with HIF-MSC enhances cell engraftment in vivo, possibly by increasing clonogenic capacity of hematopoietic cells and inducing the expression of adhesion molecules involved in graft survival in the hematopoietic niche.
STEM CELL RESEARCH & THERAPY
(2021)
Article
Biotechnology & Applied Microbiology
Hawraa Elhossaini, Mouna Hamad, Mohammad R. Irhimeh, Shirley Nakhla, Gobinath P. Rajarathnam, Ali Abbas
Summary: This study examines the effects of varying concentrations of oxygen and carbon dioxide on human bone marrow cells. The results suggest that low oxygen and high carbon dioxide conditions help maintain cell identity and stemness, and prolong cell survival. This study is important for enhancing stem cell proliferation systems while preserving their stemness.
BIOCHEMICAL ENGINEERING JOURNAL
(2022)
Article
Virology
Lindsey B. Crawford, Meaghan H. Hancock, Hillary M. Struthers, Daniel N. Streblow, Andrew D. Yurochko, Patrizia Caposio, Felicia D. Goodrum, Jay A. Nelson
Summary: This study demonstrates that CD34(+) progenitor cells derived from embryonic stem cells can serve as a model for HCMV latency, and the virus may either latently or persistently infect specific subpopulations of CD34(+) cells.
JOURNAL OF VIROLOGY
(2021)
Article
Multidisciplinary Sciences
Hannes Ausserwoeger, Georg Krainer, Timothy J. Welsh, Nels Thorsteinson, Ella de Csillery, Tomas Sneideris, Matthias M. Schneider, Thomas Egebjerg, Gaetano Invernizzi, Therese W. Herling, Nikolai Lorenzen, Tuomas P. J. Knowles
Summary: This study systematically analyzed the impact of surface patch properties on antibody nonspecificity using a designer antibody library and single-stranded DNA. The results showed that the binding affinity between antibodies and single-stranded DNA is driven mainly by a hydrophobic patch in the complementarity-determining regions. It was also found that the nonspecific binding affinity correlates with a trade-off between the hydrophobic and total charged patch areas, and that a change in formulation conditions at low ionic strengths leads to DNA-induced antibody phase separation.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2023)
Review
Biochemistry & Molecular Biology
Caitlin Molczyk, Rakesh K. Singh
Summary: Therapy resistance is a concern in treating malignant disease, as evidence suggests that a subset of cancer cells called cancer stem cells (CSCs) promote tumor survival, therapy resistance, and relapse. The inflammatory tumor microenvironment, particularly the CXCR1/2 pathway, plays a crucial role in maintaining the CSC pool. CXCR1 is known for its role as a CSC marker and potential therapeutic target in solid tumors.
Article
Hematology
Aitzkoa Lopez de lapuente Portilla, Ludvig Ekdahl, Caterina Cafaro, Zain Ali, Natsumi Miharada, Gudmar Thorleifsson, Kristijonas Zemaitis, Antton Lamarca Arrizabalaga, Malte Thodberg, Maroulio Pertesi, Parashar Dhapola, Erik Bao, Abhishek Niroula, Divya Bali, Gudmundur Norddahl, Nerea Ugidos Damboriena, Vijay G. Sankaran, Goran Karlsson, Unnur Thorsteinsdottir, Jonas Larsson, Kari Stefansson, Bjorn Nilsson
Summary: This study provides the first large-scale analysis of the genetic architecture of blood CD341 cell levels, identifying several associated loci including PPM1H and CXCR4. Functional fine-mapping suggests that the downregulation of PPM1H may be mediated through the regulation of MYB transcription factor binding site. These results warrant further investigation of PPM1H as a potential inhibition target for stem cell mobilization.
Article
Immunology
Sabrina B. Bennstein, Sandra Weinhold, Oezer Degistirici, Robert A. J. Oostendorp, Katharina Raba, Gesine Koegler, Roland Meisel, Lutz Walter, Markus Uhrberg
Summary: ILC3, particularly IL-22-secreting ILC3, play vital roles in maintaining mucosal barrier functions and controlling inflammatory diseases. A method for generating functionally competent human ILC3 from cord blood-derived CD34(+) hematopoietic progenitors on human MSCs has been developed, showing promise for potential clinical applications in treating inflammatory diseases and cancer.
FRONTIERS IN IMMUNOLOGY
(2021)
Article
Multidisciplinary Sciences
Amanda A. van Vliet, Ella Peters, Denise Vodegel, Danielle Steenmans, Monica Raimo, Susan Gibbs, Tanja D. de Gruijl, Adil D. Duru, Jan Spanholtz, Anna-Maria Georgoudaki
Summary: UCB CD34(+) progenitor cell-derived NK cells showed efficient cytotoxicity against melanoma cells, and this performance was consistent among different UCB donors and correlated with the levels of IFNg, TNF, perforin and granzyme B. Perforin and granzyme B levels predicted NK cell cytotoxic capacity. The involvement of multiple activating receptors, especially TRAIL, was identified as the mode of action for the synergistic cytotoxic NK cell activity.
Article
Cell Biology
Natarajan Aravindan, Dinesh Babu Somasundaram, Terence S. Herman, Sheeja Aravindan
Summary: Research investigated the role of CD34 in neuroblastoma progression and clinical outcomes, finding associations between CD34 expression and key indicators such as MYCN amplification, disease stage, and disease progression. The expression of CD34 in NB CSCs may influence crucial signaling pathways in the evolution of neuroblastoma.
CELL BIOLOGY AND TOXICOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Shelly Arora, Paul R. Cooper, Lara T. Friedlander, Benedict Seo, Shakila B. Rizwan, Alison M. Rich, Haizal Mohd Hussaini
Summary: This study successfully isolated and cultured cHDPCs and ncHDPCs from healthy and inflamed dental pulp tissue respectively. The growth characteristics and differentiation abilities were not significantly different between the two types of HDPCs. However, the expression of certain genes such as TLR-2 and IL-6 was significantly higher in cHDPCs, indicating an inflammatory phenotype in the inflamed dental pulp.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Engineering, Chemical
Maximilian Nisslein, Mirna Gonzalez-Gonzalez, Marco Rito-Palomares
Summary: The study investigated the influence of parameters on the partition behavior of human peripheral blood and CD34 antibody in different ATPS systems, suggesting that volume ratio has a stronger effect on the separation behavior.
SEPARATION AND PURIFICATION TECHNOLOGY
(2021)
Review
Cell Biology
Balaj Rai, Janki Shukla, Timothy D. Henry, Odayme Quesada
Summary: Ischemia with non-obstructive coronary arteries (INOCA) is a recognized disease with a high prevalence, leading to increased morbidity and mortality. Treatment of coronary microvascular dysfunction (CMD) through CD34(+) cell therapy shows promise in improving coronary flow reserve and quality of life in INOCA patients. Ongoing trials are evaluating the safety and efficacy of this novel treatment modality for INOCA with endothelial-independent CMD.
Article
Chemistry, Applied
Xiaodan Wu, Zihan Wang, Guansong Shao, Bingyang Qin, Ying Wang, Tao Wang, Zhiguo Liu, Yujie Fu
Summary: In this study, a new type of cellulose-based ionic imprinting adsorbent was developed for the selective removal of cobalt ions. The adsorbent exhibited excellent adsorption performance, high cycling stability, and preferential selectivity for cobalt ions. The simple preparation method and promising application prospects make it a promising candidate for water treatment.
REACTIVE & FUNCTIONAL POLYMERS
(2024)
Article
Chemistry, Applied
Chandirasekar Shanmugam, Vanitha Marimuthu, Nagappan Rajendiran
Summary: In this study, monodispersed gold and silver nanoparticles were synthesized using cholic acid functionalized star poly(DL-lactide) as a capping and reducing agent. The synthesized materials were found to be effective heterogeneous catalysts for the reduction reaction.
REACTIVE & FUNCTIONAL POLYMERS
(2024)
Article
Chemistry, Applied
Bianca-Iulia Ciubotaru, Mirela-Fernanda Zaltariov, Mihaela Dascalu, Adrian Bele, Alexandra Bargan, Maria Cazacu
Summary: Porous networks were obtained using polysiloxanes pendant functionalized with aminopropyl groups. The addition of certain substances, such as ammonium bicarbonate and acetic acid, promoted the porosity and covalent cross-linking of the polymer. The resulting materials exhibited high dielectric permittivity and significant electrical output signal when subjected to compression, making them suitable for capacitive pressure sensors.
REACTIVE & FUNCTIONAL POLYMERS
(2024)
Article
Chemistry, Applied
Mohamed A. Zein, Basim H. Asghar, Abdulaziz M. Almohyawi, Norah F. Alqahtani, Ahmed Alharbi, J. Alkabli, Reda F. M. Elshaarawy, Lamia A. Ismail
Summary: In this study, new amphiphilic chitosan-thyme extract-nanosilver nanocomposites were prepared, which synergistically inhibited bacterial biofilm formation and bacterial proliferation. The nanocomposites disrupted bacterial cell membranes, causing cell death, and prevented biofilm formation. In addition, the nanocomposites exhibited good biocompatibility, making them potential candidates for biomedical applications.
REACTIVE & FUNCTIONAL POLYMERS
(2024)
Review
Chemistry, Applied
Norah F. Alqahtani
Summary: This review explores the potential of Imidazolium-based ionic liquids (ImILs) in improving the solubility, stability, and functionality of chitosan, and provides an overview of the types and preparation methods of functionalized imidazolium ionic liquids (FImILs). Additionally, it discusses the various opportunities offered by FImIL-chitosan derivatives.
REACTIVE & FUNCTIONAL POLYMERS
(2024)
Article
Chemistry, Applied
Olesya N. Zabegaeva, Alexey F. Kosolapov, Sergei L. Semjonov, Mariam G. Ezernitskaya, Egor S. Afanasyev, Ivan A. Godovikov, Alexander Chuchalov, Dmitriy A. Sapozhnikov
Summary: Polyimides are commonly used as primary protective coatings for optical fibers in high-temperature applications. However, the manufacturing process is complicated due to certain drawbacks. This study shows the potential use of polyamide-imides as highly reliable protective coatings for optical fibers.
REACTIVE & FUNCTIONAL POLYMERS
(2024)
Article
Chemistry, Applied
Luis Miguel Nunez Tapia, Pascal Thebault, Laurent Bischoff, Alain Ledoux, Florian Defontaine, Olivier Lesouhaitier, Fabrice Burel
Summary: A water soluble cationic bis-cyclocarbonate was prepared through a three steps procedure, and non-isocyanate polyurethanes were prepared in acetonitrile with high yields. The resulting materials exhibited good thermal properties and hydrophilic properties. The polyurethane thermoset showed high inhibition towards bacteria, and the bis-cyclocarbonate oligomer was found to be harmless to organisms.
REACTIVE & FUNCTIONAL POLYMERS
(2024)
