Editorial Material
Biochemistry & Molecular Biology
Rafal Franski, Monika Beszterda-Buszczak
Summary: Villalva et al. evaluated the potential of yarrow extract as a treatment for H. pylori infection. They used the agar-well diffusion bioassay to test the extract's antimicrobial activity. The extract was fractionated into two fractions containing polar phenolic compounds and monoterpenes and sesquiterpenes, respectively. The phenolic compounds were identified using HPLC-ESIMS, but there were some disputed product ions.
Article
Biochemistry & Molecular Biology
Margaux Fresnais, Siwen Liang, Deniz Seven, Nevena Prodanovic, Julia Sundheimer, Walter E. Haefeli, Juergen Burhenne, Remi Longuespee
Summary: The development and validation of desorption/ionization mass spectrometric assays for drug quantification in tissue sections is crucial for their widespread application in (clinical) pharmacology. Advances in desorption electrospray ionization have demonstrated its usefulness for developing targeted quantification methods that meet validation requirements. Factors such as desorption spot morphology, analytical time, and sample surface need to be considered for successful method development. This study highlights the importance of considering desorption kinetics during DESI analyses, which can help reduce analytical time, verify solvent-based drug extraction methods, and predict feasibility of imaging assays. These findings provide valuable guidance for future development of validated DESI-profiling and imaging methods.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Review
Chemistry, Analytical
Mingxia Liu, Daiyu Miao, Shaojie Qin, Huwei Liu, Yu Bai
Summary: This review summarizes the comprehensive and essential developments of mass spectrometric immunoassay (MSIA) and its bioanalytical applications. MSIA overcomes the limitation of low ionization efficiency of biomacromolecules through mass tag-based detection, enabling highly sensitive detection and showing great potential in bioanalysis.
TRAC-TRENDS IN ANALYTICAL CHEMISTRY
(2022)
Article
Biochemical Research Methods
Herbert J. Dias, Eduardo J. Crevelin, Vinicius Palaretti, Ricardo Vessecchi, Antonio E. M. Crotti
Summary: Through negative ion ESI accurate-mass tandem and sequential mass spectrometry, it was found that deprotonated DBNs produced more diagnostic product ions than protonated molecules, providing more information and avoiding the use of additives.
RAPID COMMUNICATIONS IN MASS SPECTROMETRY
(2021)
Article
Chemistry, Analytical
Daiki Asakawa, Hajime Mizuno, Eiji Sugiyama, Kenichiro Todoroki
Summary: The study focused on the impact of liquid chromatography-tandem mass spectrometry interfaced with electrospray ionization on tryptophan-derived metabolites, revealing fragmentation during ESI. The quantitative analysis used multiple reaction monitoring technology and proposed a method for high sensitivity analysis.
Article
Chemistry, Analytical
G. Asher Newsome, Kathleen M. Martin
Summary: This study examines selected Native American woven woodsplint basketry created between 1870 and 1983 to recover traditional knowledge on their manufacture by identifying dyes or colorants. An ambient mass spectrometry system is used to non-invasively sample from intact objects, with no cutting or exposure to liquid. The analysis process involves thermal desorption, gas phase analysis, and photoionization for real-time identification of dye molecules.
ANALYTICAL CHEMISTRY
(2023)
Article
Biochemistry & Molecular Biology
Ibrahim Aissa, Aniko Kilar, Agnes Doernyei
Summary: This study investigated the fragmentation behavior of synthetic lipid A derivatives using mass spectrometry, proposing new dissociation pathways and interpreting fragment ions based on possible mechanisms. The findings contribute to a better understanding of lipid A fragmentation processes and the differentiation of isomers with single ester-linked fatty acids at different positions.
Article
Chemistry, Analytical
Zixiang Fang, Saiful M. Chowdhury
Summary: The study introduced a dual-stage neutral loss MS3 strategy for improving the large-scale identification of protein prenylation. This strategy enhances the confidence in identifying prenylation sites without prior enrichment of samples, facilitating the study of prenylation and further development in the profiling and quantification of the prenylome.
ANALYTICAL CHEMISTRY
(2021)
Article
Materials Science, Multidisciplinary
Jean-Arthur Amalian, Tathagata Mondal, Evgeniia Konishcheva, Gianni Cavallo, Benoit Eric Petit, Jean-Francois Lutz, Laurence Charles
Summary: Sequence-defined synthetic polymers are being used as a medium to store information at the molecular level, with low storage capacity of readable chains being a current limitation. Ordering short oligomers on surfaces to compose long messages is an attractive alternative, and desorption electrospray ionization (DESI) is shown to be suitable for bidimensional data storage.
ADVANCED MATERIALS TECHNOLOGIES
(2021)
Article
Chemistry, Analytical
Fong-Fu Hsu
Summary: This study utilized HCD MS2 spectra to investigate the fragmentation processes of ceramide molecules, allowing for accurate definition of ceramide structures based on observed sets of fragment ions reflecting the fatty acyl chain and LCB. The approach permits differentiation of isomeric structures and detailed characterization of ceramide species in biological samples.
ANALYTICA CHIMICA ACTA
(2021)
Article
Biochemical Research Methods
Felix-Levin Hormann, Simon Sommer, Sven Heiles
Summary: Phospholipids are crucial components of eukaryotic cell membranes and undergo structural changes during metabolic states. These structural variations are associated with disease states and specific organisms. This study demonstrates that all analyzed phospholipid classes form doubly charged lipid-metal ion complexes during electrospray ionization, which can be utilized for lipid class assignment and fatty acid identification. The use of water free methanol and divalent metal salts greatly enhances the abundance of these complexes. This method allows for the differentiation of fatty acid isomers and relative quantitation of these isomeric compounds.
JOURNAL OF THE AMERICAN SOCIETY FOR MASS SPECTROMETRY
(2023)
Review
Pharmacology & Pharmacy
Manman Qin, Yuqing Qian, Lu Huang, Chao Zhong, Mingdong Li, Jun Yu, Huanwen Chen
Summary: Extraction electrospray ionization mass spectrometry (EESI-MS) enables effective ionization of trace molecules in complex samples without sample pretreatment, providing high sensitivity, selectivity, and response speed. It also allows for advanced functionalization of high-energy molecular species. This article systematically reviews the effects of operating conditions on the analytical performance of EESI-MS for various pharmaceutical compounds and introduces its potential application in molecular functionalization for drug discovery.
FRONTIERS IN PHARMACOLOGY
(2023)
Article
Biochemical Research Methods
Allison Brookhart, Mahika Arora, Michael McCullagh, Ian D. Wilson, Robert S. Plumb, Johannes P. C. Vissers, Nikunj Tanna
Summary: Mobile phase selection is crucial in LC-MS studies, affecting various aspects such as retention, selectivity, ionization, and detection limits. This study evaluated the effect of solvent composition on ESI response for 240 small molecular weight drugs. Surface area and surface charge-related features were found to be the main factors influencing ESI response. The composition of the mobile phase had a less significant impact, with some compounds showing a pH effect.
JOURNAL OF CHROMATOGRAPHY A
(2023)
Article
Polymer Science
Tomos E. Morgan, Thomas G. Floyd, Bryan P. Marzullo, Christopher A. Wootton, Mark P. Barrow, Anthony W. T. Bristow, Sebastien Perrier, Peter B. O'Connor
Summary: Understanding the modification of synthetic polymer structures is crucial for accurate synthesis and applications. In this study, partially hydrolyzed poly(2-oxazoline) species were produced to form poly[(2-polyoxazoline)-co-(ethylenimine)] (P(EtOx-co-EI)) copolymers. Mass spectrometry analysis revealed that the distribution of the hydrolyzed product (EI) within the copolymer was influenced by the choice of terminus, with permanent hydrolysis observed at an OH terminus.
Article
Biochemical Research Methods
Dilip Kumar Singh, Siddheshwar Kisan Chauthe, Prakash Anjanappa, Sumit Kumar, Ruba A. Arulraj, Raju Gajjela, Noor Mohammed, Venkatesh T. Kanthappa, Ananta Karmakar, Arun Kumar Gupta, Lakshmikant Bajpai, Muralidhararao Bagadi, Arvind Mathur
Summary: The study successfully differentiated pairs of N,N'-substituted urea positional isomers using electrospray ionization tandem mass spectrometry, demonstrating the method's potential for characterizing a series of derivatives.
RAPID COMMUNICATIONS IN MASS SPECTROMETRY
(2021)
Article
Biochemical Research Methods
Ang Liu, John Lute, Huidong Gu, Bonnie Wang, Kevin J. Trouba, Mark E. Arnold, Anne-Francoise Aubry, Jian Wang
JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
(2015)
Article
Biochemical Research Methods
Ang Liu, Alexander Kozhich, David Passmore, Huidong Gu, Richard Wong, Frank Zambito, Vangipuram S. Rangan, Heather Myler, Anne-Francoise Aubry, Mark E. Arnold, Jian Wang
JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
(2015)
Article
Biochemical Research Methods
Jian Wang, Huidong Gu, Ang Liu, Alexander Kozhich, Vangipuram Rangan, Heather Myler, Linlin Luo, Richard Wong, Huadong Sun, Bonnie Wang, Heather E. Vezina, Shrikant Deshpande, Yan Zhang, Zheng Yang, Timothy V. Olah, Anne-Francoise Aubry, Mark E. Arnold, Renuka Pillutla, Binodh DeSilva
Article
Pharmacology & Pharmacy
Ajit S. Narang, Anand Balakrishnan, John Morrison, Jinjiang Li, Jennifer Wang, Huidong Gu, Katrina Taylor, Kenneth Santone, Jon Ehrmann, Sophie Beyer, Xujin Lu, Rod Ketner, Jennifer Pizzano, Tami Orcutt, Eric Shields, Heidi Dulac, Sharon Aborn, Margaret Batchelder, Kimberley Lentz
EUROPEAN JOURNAL OF PHARMACEUTICS AND BIOPHARMACEUTICS
(2017)
Article
Biochemical Research Methods
Hongwei Zhang, Huidong Gu, Petia Shipkova, Eugene Ciccimaro, Huadong Sun, Qihong Zhao, Timothy V. Olah
Article
Biochemical Research Methods
Yue Zhao, Huidong Gu, Naiyu Zheng, Jianing Zeng
Article
Chemistry, Analytical
Huidong Gu, Yue Zhao, Marissa DeMichele, Naiyu Zheng, Yan J. Zhang, Renuka Pillutla, Jianing Zeng
ANALYTICAL CHEMISTRY
(2019)
Article
Chemistry, Analytical
Huidong Gu, Yue Zhao, Marissa DeMichele, Naiyu Zheng, Yan J. Zhang, Renuka Pillutla, Jianing Zeng
ANALYTICAL CHEMISTRY
(2019)
Article
Chemistry, Analytical
Naiyu Zheng, Ian M. Catlett, Kristin Taylor, Huidong Gu, Mark A. Pattoli, Robert J. Neely, Wenying Li, Alban Allentoff, Xiling Yuan, Eugene Ciccimaro, Ming Yao, Bethanne M. Warrack, James R. Burke, Yan J. Zhang, Renuka Pillutla, Jianing Zeng
ANALYTICAL CHEMISTRY
(2019)
Article
Chemistry, Analytical
Long Yuan, Huidong Gu, Jianing Zeng, Renuka C. Pillutla, Qin C. Ji
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2020)
Article
Pharmacology & Pharmacy
Weiguo Cai, Tarek A. Leil, Leonid Gibiansky, Murli Krishna, Hongwei Zhang, Huidong Gu, Huadong Sun, John Throup, Subhashis Banerjee, Ihab Girgis
CLINICAL PHARMACOLOGY IN DRUG DEVELOPMENT
(2020)
Article
Chemistry, Analytical
Yue Zhao, Huidong Gu, Jennifer Postelnek, Marissa DeMichele, Long Yuan, Yan J. Zhang, Jianing Zeng
ANALYTICA CHIMICA ACTA
(2020)
Article
Chemistry, Analytical
Naiyu Zheng, Kristin Taylor, Huidong Gu, Rasa Santockyte, Xi-Tao Wang, Jean McCarty, Olufemi Adelakun, Yan J. Zhang, Renuka Pillutla, Jianing Zeng
ANALYTICAL CHEMISTRY
(2020)
Editorial Material
Biochemical Research Methods
Mark E. Arnold, John L. Allinson, Steven P. Piccoli
Article
Medicine, Research & Experimental
Yue Zhao, Huidong Gu, Jianing Zeng