期刊
RAPID COMMUNICATIONS IN MASS SPECTROMETRY
卷 23, 期 10, 页码 1419-1425出版社
WILEY
DOI: 10.1002/rcm.4016
关键词
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资金
- Grant Agency of the Czech Republic [203/Q9/0256]
- GACR [202/ 09/0800]
The reactions of carbon dioxide, CO2, with the precursor ions used for selected ion flow tube mass spectrometry, SIFT-MS, analyses, viz. H3O+, NO+ and O-2(+), are so slow that the presence of CO2 in 2 exhaled breath has, until recently, not had to be accounted for in SIFT-MS analyses of breath. This has, however, to be accounted for in the analysis of acetaldehyde in breath, because an overlap occurs of the monohydrate of protonated acetaldehyde and the weakly bound adduct ion, H3O+CO2, formed by the slow association reaction of the precursor ion H3O+ With CO2 molecules. The understanding of the kinetics of formation and the loss rates of the relevant ions gained from experimentation using the new generation of more sensitive SIFT-MS instruments now allows accurate quantification of CO2 in breath using the level of the H3O+CO2 adduct ion. However, this is complicated by the rapid reaction of H3O+CO2 with water vapour molecules, H2O, that are in abundance in exhaled breath. Thus, a study has been carried out of the formation of this adduct ion by the slow three-body association reaction of H3O+ with CO2 and its rapid loss in the two-body reaction with H2O molecules. It is seen that the signal level of the H3O+CO2 adduct ion is sensitively dependent on the humidity (H2O concentration) of the sample to be analysed and a functional form of this dependence has been obtained. This has resulted in an appropriate extension of the SIFT-MS software and kinetics library that allows accurate measurement of CO2 levels in air samples, ranging from very low percentage levels (0.03% typical of tropospheric air) to the 6% level that is about the upper limit in exhaled breath. Thus, the level of CO2 can be traced through single time exhalation cycles along with that of water vapour, also close to the 6% level, and of trace gas metabolites that are present at only a few parts-per-billion. This has added a further dimension to the analysis of major and trace compounds in breath using SIFT-MS. Copyright (C) 2009 John Wiley & Sons, Ltd.
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