Review
Chemistry, Analytical
A. Fernez Asensio, E. Alvarez-Gonzalez, A. Rodriguez, L. M. Sierra, E. Blanco-Gonzalez
Summary: Dysregulation of TCA cycle enzymes leads to abnormal accumulation of oncometabolites, which contribute to cancer formation and progression. GC-MS and LC-MS are powerful analytical tools for accurate identification and determination of these metabolites.
ANALYTICA CHIMICA ACTA
(2021)
Article
Food Science & Technology
Yuan Wang, Lijie Xing, Jinlei Zhang, Xiaoning Ma, Rui Weng
Summary: To achieve comprehensive analysis of phenolic compounds in honey, a simple and reliable method using HPLC-MS/MS was developed. 24 compounds were simultaneously analyzed with good linearity and high sensitivity. The method proved to be suitable for the analysis of phenolic compounds in honey samples.
LWT-FOOD SCIENCE AND TECHNOLOGY
(2023)
Article
Health Care Sciences & Services
Rita Barone, Jean Bastin, Fatima Djouadi, Indrapal Singh, Mohammad Azharul Karim, Amrit Ammanamanchi, Patrick John McCarty, Leanna Delhey, Rose Shannon, Antonino Casabona, Renata Rizzo, Richard Eugene Frye
Summary: Patients with autism spectrum disorder may have elevated blood acyl-carnitine levels, indicating mitochondrial fatty acid beta-oxidation impairment. Treatment with resveratrol significantly increased mitochondrial fatty acid oxidation activity in all study groups, with the most pronounced effects observed in patients with more severe symptoms.
JOURNAL OF PERSONALIZED MEDICINE
(2021)
Article
Multidisciplinary Sciences
Dapeng Chen, Noella A. Bryden, Wayne A. Bryden, Michael McLoughlin, Dexter Smith, Alese P. Devin, Emily R. Caton, Caroline R. Haddaway, Michele Tameris, Thomas J. Scriba, Mark Hatherill, Sophia Gessner, Digby F. Warner, Robin Wood
Summary: Human breath contains trace amounts of non-volatile organic compounds (NOCs) which could be used as markers of individual health. In this study, metabolites and lipids in exhaled breath aerosol (EBA) samples were analyzed to identify molecular signatures of active tuberculosis (TB). Thirteen metabolite and nine lipid markers were identified with statistically different values between individuals with active TB and healthy controls. Importantly, EBA lipid profiles can effectively distinguish the two sample types, indicating the diagnostic potential of the identified molecules.
SCIENTIFIC REPORTS
(2022)
Article
Chemistry, Analytical
Jose Grau, Maria Moreno-Guzman, Luis Arruza, Miguel angel Lopez, Alberto Escarpa, Alberto Chisvert
Summary: Miniaturized magnetic-based pipette tip microextraction is a sample preparation approach for microsamples. It involves dispersing a small amount of magnetic sorbent material in a low-volume sample to trap the target analytes, followed by using a pipette tip with a neodymium magnet inside to retrieve the sorbent. The method was successfully applied to determine free cortisol in serum and urine samples from low birth weight preterm newborns.
Article
Food Science & Technology
Madina Mamedova, Mereke B. Alimzhanova
Summary: This study focused on the analysis of volatile and semi-volatile compounds in honey using vacuum-assisted solid-phase microextraction and gas chromatography-mass spectrometry (Vac-SPME/GC-MS) for the first time. The Vac-SPME method was compared with the conventional HS-SPME method, and its application in characterizing the volatile and semi-volatile profile of honey was explored. Analysis of ten multiflower honey samples from mountainous and steppe regions revealed significant geographical variation in honey composition, highlighting the importance of differentiation.
FOOD ANALYTICAL METHODS
(2023)
Article
Biochemistry & Molecular Biology
Zeyang Liu, Susu Pan, Peize Wu, Ming Li, Dapeng Liang
Summary: Beta-casein, a protein found in milk and dairy products, has two variants called A1 and A2. A1 beta-casein is believed to have negative effects on human health. Distinguishing between A1 and A2 beta-casein is challenging due to only a single amino acid variation at the 67th position. This study developed a novel method using thermolytic peptides to determine the presence of A1 and A2 beta-casein in milk samples, offering high specificity, sensitivity, and efficiency.
Article
Endocrinology & Metabolism
Jun Kou, Chunyang He, Lin Cui, Zhengping Zhang, Wei Wang, Li Tan, Da Liu, Wei Zheng, Wei Gu, Ning Xia
Summary: The study on Chinese postmenopausal women with osteoporosis identified potential biomarkers related to metabolic pathway disorders, providing new insights for early diagnosis. The findings suggest that metabonomic analysis has great potential for application in postmenopausal women with osteoporosis.
FRONTIERS IN ENDOCRINOLOGY
(2022)
Article
Chemistry, Analytical
Deniss Fedorenko, Antons Podjava, Arvis Prikulis, Vadims Bartkevics
Summary: A new method using nanoflow liquid chromatography with Orbitrap mass spectrometry has been developed for quantitative analysis of biomarkers and pharmaceutical compounds in wastewater. The method employs an easy dilute-and-shoot approach for sample preparation and utilizes tetrabutylammonium bromide as an ion pair reagent to improve the retention of ionic and highly polar compounds. The new method demonstrates low matrix effects, high sensitivity, low injection volume and solvent consumption, and the ability to analyze diverse polar and ionic analytes within one run.
JOURNAL OF SEPARATION SCIENCE
(2023)
Article
Biochemistry & Molecular Biology
Sieglinde Zelzer, Andreas Meinitzer, Markus Herrmann, Walter Goessler, Dietmar Enko
Summary: This study aimed to determine vitamin D metabolites in serum and cerebrospinal fluid (CSF) from individuals with varying brain-CSF-barrier (BCB) function. The findings revealed higher concentrations of vitamin D metabolites in CSF of patients with impaired BCB function, potentially due to passive diffusion across the BCB.
Article
Food Science & Technology
Liyuan Wang, Zhengyan Hu, Jing Chen, Tianjiao Wang, Pinggu Wu, Ying Ying
Summary: This study developed a method to simultaneously detect a dozen preservatives in pastry using gas chromatography-mass spectrometry. The preservatives showed good linearity within the range of 1.0-50 μg/mL, with detection limits of 0.04-2.00 mg/kg and quantification limits of 0.12-6.67 mg/kg. The method is suitable for the determination of 12 preservatives in pastry.
Article
Materials Science, Biomaterials
Yan Zhou, Xvelian Li, Yuewei Zhao, Shouzhi Yang, Lin Huang
Summary: Biothiols play important roles in physiological and pathological processes. Plasmonic alloys were synthesized for the detection of biothiols in mass spectrometry. These alloys enhanced detection sensitivity and selectivity, allowing for direct detection of biothiols without enrichment or separation. Internal standards were introduced for accurate quantification, and a monitoring platform for the oxidation-reduction reaction of glutathione was established. This work contributes to the development of plasmonic-based molecular tools for biothiol detection in real-case applications.
JOURNAL OF MATERIALS CHEMISTRY B
(2023)
Article
Chemistry, Analytical
Shakiba Zeinali, Chiranjit Ghosh, Janusz Pawliszyn
Summary: Breath-composition analysis is a non-invasive method for early disease diagnosis and exposure history investigation. To overcome the limitations of aerosol nature and low concentrations of volatile organic compounds in breath samples, researchers applied a needle-trap device and electrospun polyacrylonitrile filter for breath characterization. The results showed that the needle-trap device had advantages in capturing droplets and extracting free gas-phase components.
ANALYTICA CHIMICA ACTA
(2022)
Article
Environmental Sciences
Aaron J. Pandopulos, Richard Bade, Benjamin J. Tscharke, Jake W. O'Brien, Bradley S. Simpson, Jason M. White, Cobus Gerber
Summary: This study evaluated catecholamine metabolites as potential population biomarkers and found high correlations and similarities in some wastewater catchments in Australia, indicating their suitability as markers for future studies.
SCIENCE OF THE TOTAL ENVIRONMENT
(2021)
Article
Chemistry, Analytical
Julius Agongo, Michael Armbruster, Christopher Arnatt, James Edwards
Summary: The analysis of metabolites in electrospray mass spectrometry is complicated due to their structural and charge state heterogeneity. This study presents a sequential dual-stage derivatization and capillary RP LC-ESI-MS analysis method to reduce complications and improve the accuracy of signal response and quantitation. The derivatization process increases the proton affinity and hydrophobicity of analytes, resulting in improved detection and identification of metabolites in various samples.
ANALYTICAL METHODS
(2022)