Article
Chemistry, Analytical
Jonatan Dias, Sonia Herrera Lopez, Hans Mol, Andre de Kok
Summary: Hydrophilic interaction liquid chromatography is recognized as an important solution for separating polar compounds not amenable to reverse phase chromatography. This study evaluated three different hydrophilic liquid chromatography stationary phases for the determination of 14 highly polar anionic molecules, showcasing different matrix effects and interactions between compounds. The use of isotopically labeled internal standards enabled acceptable quantitative performance and identification down to 0.02mgkg(-1) for most compounds.
JOURNAL OF SEPARATION SCIENCE
(2021)
Article
Chemistry, Applied
Xixi Jia, Xiaowei Li, Liang Zhao, Saiwa Liu, Zhinan Wang, Jingjing Du, Lirui Ge, Kunxia Zhao, Lu Tian, Jian Xu, Zuhao Yan, Jiangyi Ding, Xi Xia
Summary: A sensitive and selective method using hydrophilic interaction chromatography combined with tandem mass spectrometry was developed to detect 18 aminoglycosides in bovine milk. Aminoglycosides were extracted and isolated from milk using a mixture of trichloroacetic acid, ammonium acetate, ethylenediaminetetraacetic acid, and sodium chloride, and an Oasis HLB solid-phase extraction cartridge. The method showed satisfactory separation of the analytes with detection and quantification limits ranging from 0.05-2 μg/kg and 0.1-5 μg/kg, respectively. The method was successfully applied to detect neomycin in one fresh bovine milk sample, and it provides a powerful tool for the surveillance of aminoglycosides in bovine milk.
JOURNAL OF FOOD COMPOSITION AND ANALYSIS
(2023)
Article
Chemistry, Analytical
Maha Yahya, Sare Kesekler, Ilknur Durukan, Cigdem Arpa
Summary: A new method was developed in this study to determine trace amounts of lead and cadmium in hair dye and henna samples, with high enhancement factors and low detection limits achieved through optimization of various parameters. The influence of sample matrix on the determination of lead and cadmium was successfully addressed, enabling accurate quantification of lead and cadmium content in different samples.
ANALYTICAL METHODS
(2021)
Article
Biochemical Research Methods
Florencia Jesus, Adrian Rosa Garcia, Tommaso Stecconi, Victor Cutillas, Amadeo Rodriguez Fernandez-Alba
Summary: The analysis of highly polar pesticides is challenging due to their unique physicochemical properties. In this study, a successful analytical methodology for the extraction, clean-up, and direct determination of 11 highly polar anionic pesticides in complex food matrices was developed and validated. The application of hydrophilic interaction liquid chromatography coupled to tandem mass spectrometry, as well as solid-phase extraction and micro-solid-phase extraction employing strong anion exchange (SAX) cartridges, enabled accurate and sensitive detection of these compounds. The validated methodologies showed great potential for routine control of complex samples such as honey and pollen.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2023)
Article
Biochemical Research Methods
J. Lopez-Vazquez, L. Perez-Mayan, V. Fernandez-Fernandez, R. Cela, I. Rodriguez
Summary: An automated procedure was developed for the simultaneous determination of six anionic pesticides, including glyphosate and its transformation product aminomethylphosphonic acid, in environmental water samples. The method combined on-line concentration of water samples, anion-exchange liquid chromatography separation, and tandem mass spectrometry for selective determination. The method provided low limits of quantification and was applied to analyze samples from three watersheds in Galicia, Spain. The results showed the presence of Fosetyl, AMPA, and GLY, with higher levels and seasonal variations observed in rivers affected by sewage treatment plant discharges.
JOURNAL OF CHROMATOGRAPHY A
(2023)
Article
Biochemical Research Methods
Ming Huang, Xiaobin Xu, Haibo Qiu, Ning Li
Summary: In this study, a generic HILIC-MS/MS method was developed for in-depth characterization of nucleic acid therapeutic modalities. High-resolution mass spectrometry was used to determine the deconvoluted masses of oligonucleotide and siRNA standards and their impurities. Optimized higher-energy C-trap dissociation was used to improve the sequence coverage of DNA and RNA oligonucleotides, achieving higher on-column sensitivity for phosphorothioate oligonucleotide analysis.
JOURNAL OF CHROMATOGRAPHY A
(2021)
Article
Chemistry, Analytical
Na Hang, Yang Yang, Yuyue Zang, Wanning Zhao, Jing Tao, Songqing Li
Summary: This study assessed the use of magnetic biosorbents in dispersive solid-phase extraction for the determination of pesticides in water samples. It is the first time that magnetic cork composites are used as an adsorbent in this extraction. The advantages of magnetic cork composites include their density regulation and high surface areas. The method showed good recoveries and detection limits, indicating that Fe3O4/cork magnetic composites can be used as efficient and eco-friendly biosorbents in dispersive solid-phase extraction for the determination of pesticides in water samples.
ANALYTICAL METHODS
(2023)
Article
Chemistry, Analytical
Zongying Li, Shuxiang Li, Feifang Zhang, Huiliang Geng, Bingcheng Yang
Summary: The novel amide functionalized polar stationary phase exhibits favorable hydrophilicity, excellent separation ability, and outstanding hydrolytic stability, with minimal baseline drift under gradient elution and good selectivity in the separation of isomers and homologue sugars.
Article
Environmental Sciences
Wenhui Qiu, Haiyang Shao, Weifeng Jin, Ying Xiong, Bentuo Xu, Bei Chen
Summary: The study identified 102 semivolatile organic compounds in the main rivers of Shenzhen, including organochlorine and organophosphorus pesticides, with diazinon being the most frequent compound detected. The concentrations of SVOCs in water and sediment were relatively stable over the years, with organochlorine pesticides primarily coming from the degradation of DDT and organophosphorus pesticides being mainly from cumulative content.
ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH
(2021)
Article
Biochemical Research Methods
Elif Ozturk Er, Belma Ozbek, Sezgin Bakirdere
Summary: A novel analytical method was described for the determination of underivatized amino acids in human plasma and urine samples, achieving accurate results through liquid chromatography-tandem mass spectrometry, demonstrating high reliability and reproducibility.
JOURNAL OF CHROMATOGRAPHY A
(2021)
Article
Chemistry, Applied
L. Perez-Mayan, M. Ramil, R. Cela, I. Rodriguez
Summary: The study utilized LC-MS/MS combined with online solid-phase extraction (SPE) strategy to detect potential pesticide residues in wine. The method showed high accuracy and provided good recovery rates for 90% of compounds under various parameter conditions.
JOURNAL OF FOOD COMPOSITION AND ANALYSIS
(2021)
Article
Chemistry, Analytical
Dan Fei, Mengzhi Wang, Yujie Hou, Min Xie, Yaomin Zhou, Youhua Zhao, Lei Wu, Jun Xu
Summary: The study presented a rapid and reliable method for simultaneous determination of organophosphorus pesticides in porcine hair, with linear relationships between 1.0 to 100 µg/kg and high accuracy and precision.
ANALYTICAL LETTERS
(2022)
Article
Chemistry, Analytical
M. Mar Aparicio-Muriana, Rocio Carmona-Molero, Francisco J. Lara, Ana M. Garcia-Campana, Monsalud del Olmo-Iruela
Summary: The development of a HILIC-MS/MS multiclass method for simultaneous analysis of eight cyanotoxins in reservoir water samples is described in this work, providing a reliable and sensitive analytical approach for cyanotoxin analysis. The study shows that a tandem solid-phase extraction system can effectively extract and preconcentrate different classes of cyanotoxins.
Article
Food Science & Technology
Salma Akter Mou, Rafiza Islam, Mohammad Shoeb, Nilufar Nahar
Summary: A fast and highly sensitive analytical method based on UHPLC-MS/MS was developed for the quantitative determination of CAP in poultry meat and beef samples. The method was validated for linearity, accuracy, precision, and specificity, showing satisfactory results. It proved to be efficient for routine analysis of CAP in various samples tested.
FOOD SCIENCE & NUTRITION
(2021)
Article
Biochemical Research Methods
Elias Iturrospe, Katyeny Manuela Da Silva, Begona Talavera Andujar, Matthias Cuykx, Joost Boeckmans, Tamara Vanhaecke, Adrian Covaci, Alexander L. N. van Nuijs
Summary: This study evaluated different chromatographic columns for analyzing polar metabolites, selecting two new generations of zwitterionic HILIC columns for further evaluation. Using a tree-based method optimization, the study investigated the chromatographic factors affecting retention mechanisms of polar metabolites and demonstrated high coverage of metabolic classes. The optimized methods showed potential for untargeted purposes, with high-quality features ranging from 3,755 to 5,402 for various biological matrices.
JOURNAL OF CHROMATOGRAPHY A
(2021)