Article
Biochemical Research Methods
Olga S. Ovchinnikova, Matthias Lorenz, Ryan B. Wagner, Ron M. A. Heeren, Roger Proksch
Summary: The method uses mechanical sampling and collection of surface materials, making real-time analysis directly from the AFM probe tip possible. By faster thermal desorption and concentration of gas-phase analytes from a single heating pulse, higher signal levels are achieved from a single sampling spot.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2021)
Article
Chemistry, Analytical
Xufang Hu, Zongping Wang, Haolin Chen, An Zhao, Nianrong Sun, Chunhui Deng
Summary: An advanced metabolic analysis tool based on highly porous metal oxides derived from metal-organic frameworks (MOFs) is developed in this study. The tool successfully differentiates and stages renal cell carcinoma (RCC), as well as identifies the size of tumors. This achievement shows promising prospects for designer matrix-based analytical tools in disease diagnosis.
ANALYTICAL CHEMISTRY
(2022)
Article
Chemistry, Multidisciplinary
Ouyang Dan, Chuanzhe Wang, Chao Zhong, Juan Lin, Gang Xu, Guane Wang, Zian Lin
Summary: In this study, novel LDI-MS substrates were designed to enhance the sensitivity of mass signals and achieve high accuracy diagnosis of different saccharides and central precocious puberty (CPP).
Article
Chemistry, Analytical
Alex Gomez-Gomez, Jose Rodriguez-Morato, Noemi Haro, Judith Marin-Corral, Joan Ramon Masclans, Oscar J. Pozo
Summary: Metabolomics, especially untargeted metabolomics, has become the gold standard for biomarker discovery. This study developed a strategy based on derivatization and LC-MS/MS detection for the untargeted determination of carbonyl-containing metabolites. The usefulness of this guided metabolomics approach was demonstrated in the discovery of potential biomarkers for COVID-19 severity.
ANALYTICA CHIMICA ACTA
(2022)
Article
Chemistry, Analytical
Xian-Na Wang, Bin Li
Summary: Gold nanoparticles/thiol-beta-cyclodextrin-functionalized TiO2 nanowires were prepared to enhance the performance of SALDI MS and MSI, showing superior detection sensitivity and repeatability.
ANALYTICAL CHEMISTRY
(2022)
Article
Materials Science, Multidisciplinary
Jean-Arthur Amalian, Tathagata Mondal, Evgeniia Konishcheva, Gianni Cavallo, Benoit Eric Petit, Jean-Francois Lutz, Laurence Charles
Summary: Sequence-defined synthetic polymers are being used as a medium to store information at the molecular level, with low storage capacity of readable chains being a current limitation. Ordering short oligomers on surfaces to compose long messages is an attractive alternative, and desorption electrospray ionization (DESI) is shown to be suitable for bidimensional data storage.
ADVANCED MATERIALS TECHNOLOGIES
(2021)
Article
Biochemical Research Methods
Gwangbin Lee, Sangwon Cha
Summary: Nano-DESI MS has been explored in the forensic chemical analysis of ink directly from handwriting on paper, showing the potential for changing mass spectral profiles over time to reflect the differential distribution of analytes in a vertical direction. The study confirmed the capability of the depth-dependent analysis of nano-DESI MS.
JOURNAL OF THE AMERICAN SOCIETY FOR MASS SPECTROMETRY
(2021)
Article
Chemistry, Analytical
Monica Lin, Molly S. Blevins, Marta Sans, Jennifer S. Brodbelt, Livia S. Eberlin
Summary: In this study, solvent-based ambient ionization mass spectrometry techniques were used to investigate the extraction processes of lipids in biological tissues. The results suggest that the physicochemical properties of the solvent systems and the extraction time significantly influence the molecular extraction and detection of lipids.
ANALYTICAL CHEMISTRY
(2022)
Article
Chemistry, Analytical
Lea Ledoux, Yanis Zirem, Florence Renaud, Ludovic Duponchel, Michel Salzet, Nina Ogrinc, Isabelle Fournier
Summary: This study directly compared water-assisted laser desorption ionization (WALDI) and matrix-assisted laser desorption ionization (MALDI) mass spectrometry imaging, with MALDI as the benchmark for label-free molecular tissue analysis in biomedical research. The results showed that the two techniques are very similar in lipidomic analysis, especially when using norharmane MALDI matrix in negative ion mode. This similarity allows for the integration of data from both techniques to increase dataset and improve classification models.
Article
Chemistry, Analytical
A. Arrizabalaga-Larranaga, S. Epigmenio-Chamu, F. J. Santos, E. Moyano
Summary: This paper describes the development of a rapid and sensitive method for detecting banned dyes in spice samples using UHPLC-MS/MS technology combined with three atmospheric pressure ionization sources. The proposed method demonstrated good performance in sample analysis, with low detection limits, good precision, and accurate quantitation.
ANALYTICA CHIMICA ACTA
(2021)
Review
Spectroscopy
Carla Harkin, Karl W. Smith, Faye L. Cruickshank, C. Logan Mackay, Bryn Flinders, Ron M. A. Heeren, Tara Moore, Simon Brockbank, Diego F. Cobice
Summary: Mass spectrometry imaging (MSI) combines molecular and spatial information, making it a valuable tool in various applications. Chemical derivatization (CD) has proven to be an effective solution for ionization issues. On-tissue chemical derivatization (OTCD) is a powerful tool for studying the spatial distribution of poorly ionizable molecules within tissues.
MASS SPECTROMETRY REVIEWS
(2022)
Article
Chemistry, Analytical
Ke Min, Xuqian Weng, Piao Long, Ming Ma, Bo Chen, Shouzhuo Yao
Summary: The study established a rapid in-situ detection method for PAEs in face masks, allowing identification and quantification of 13 kinds of PAEs with low detection limits. Compared to traditional methods, this approach offers advantages of short analysis time, low solvent consumption, and quick screening of PAEs in face masks.
Article
Biochemical Research Methods
Ane Arrizabalaga-Larranaga, Pedro de Juan-de Juan, Claudia Bressan, Mercedes Vazquez-Espinosa, Ana Gonzalez-de-Peredo, F. Javier Santos, Encarnacion Moyano
Summary: This study developed a sensitive and selective UHPLC-MS/MS method for the detection of 23 PAAs, showing the advantage of APCI as an alternative method for the analysis of PAAs. The developed method was applied to the analysis of kitchenware samples, where PAAs concentrations above the legislated limit were detected in 18 out of 20 samples.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2022)
Article
Spectroscopy
Sunil P. Badal, Paul B. Farnsworth, George C-Y Chan, Brian T. Molnar, Jessica R. Hellinger, Jacob T. Shelley
Summary: Plasma-based ambient desorption/ionization sources often use helium as the primary discharge gas, but studies show advantages of mixed-gas plasmas. Adding H-2 to helium can decrease reagent-ion signals while preserving analyte-ion signals. Nitrogen addition increases negative reagent ions for enhanced ion signal of specific compounds. Various molecular gases impact emission characteristics and plasma parameters in mixed-gas systems.
SPECTROCHIMICA ACTA PART B-ATOMIC SPECTROSCOPY
(2021)
Article
Biochemical Research Methods
Maximilian Heide, Cristian C. Escobar-Carranza, Carsten Engelhard
Summary: Ambient desorption/ionization mass spectrometry (ADI-MS) is a rapid screening tool that allows the analysis of samples without sample preparation. However, quantitative analysis of analytes in complex matrices remains a challenge. In this study, a carefully selected surface and co-deposited isotopically labeled standards were found to significantly improve the capabilities of FAPA-HR-MS for direct quantitative analysis. The use of TLC-based surfaces as sample carriers was shown to be an attractive tool for fast and efficient mass spectrometric investigations of complex samples.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2022)
Review
Spectroscopy
Giuseppe Paglia, Andrew J. Smith, Giuseppe Astarita
Summary: Researchers are utilizing ion mobility mass spectrometry (IM-MS) for metabolomics and lipidomics applications, which offers advantages such as measuring collision cross-section values, improving peak capacity and signal-to-noise ratio, and coupling with various fragmentation modes. These advancements provide a more robust tool for identification, quantification, and structural characterization in the field.
MASS SPECTROMETRY REVIEWS
(2022)
Article
Microbiology
Pierluigi Reveglia, Maria Luisa Raimondo, Marco Masi, Alessio Cimmino, Genoveffa Nuzzo, Gaetano Corso, Angelo Fontana, Antonia Carlucci, Antonio Evidente
Summary: This study used LC-MS/MS metabolomics to investigate the secondary metabolites produced by Phaeoacremonium species and their phytotoxicity. The toxic effects varied among fungal isolates, and the amounts of isosclerone and scytalone also differed.
Article
Pharmacology & Pharmacy
Sissi Dolci, Loris Mannino, Emanuela Bottani, Alessandra Campanelli, Marzia Di Chio, Stefania Zorzin, Giulia D'Arrigo, Alessia Amenta, Agnese Segala, Giuseppe Paglia, Vanna Denti, Guido Fumagalli, Enzo Nisoli, Alessandra Valerio, Claudia Verderio, Giuseppe Martano, Francesco Bifari, Ilaria Decimo
Summary: Rewiring cellular metabolism to recover oxidative metabolism may be an innovative therapeutic strategy for the treatment of spinal cord injury (SCI). In this study, oral administration of metabolic precursors enhanced spinal cord oxidative metabolism, activated the mTORC1 anabolic pathway, increased mitochondrial mass and ROS defense, effectively preventing neural cell apoptosis. These molecular and histological modifications ultimately led to a significant improvement in motor functions.
PHARMACOLOGICAL RESEARCH
(2022)
Article
Virology
Luigia Trabace, Lorenzo Pace, Maria Grazia Morgese, Isabel Bianca Santo, Domenico Galante, Stefania Schiavone, Dora Cipolletta, Anna Maria Rosa, Pierluigi Reveglia, Antonio Parisi, Paolo Tucci, Giovanni Pepe, Rodolfo Sacco, Maria Pia Foschino Barbaro, Gaetano Corso, Antonio Fasanella
Summary: The Delta variant has raised concerns about its ability to evade SARS-CoV-2 vaccines. This study evaluated the neutralizing response of 172 Italian healthcare workers three months after receiving the Comirnaty vaccine. The results showed that the vaccine provides sustained neutralizing antibody activity against the Alpha variant, but it is less effective against the Gamma and even less against the Delta variants.
Article
Virology
Monica Gelzo, Filippo Scialo, Sara Cacciapuoti, Biagio Pinchera, Annunziata De Rosa, Gustavo Cernera, Marika Comegna, Lorella Tripodi, Nicola Schiano Moriello, Mauro Mormile, Gabriella Fabbrocini, Roberto Parrella, Gaetano Corso, Ivan Gentile, Giuseppe Castaldo
Summary: Profound clinical differences were observed between the first and second waves of COVID-19 in Europe. Nitric oxide (NO) may have a positive impact on patients with SARS-CoV-2 infection. The study found that in the first wave, patients had higher levels of serum iNOS, IL-6, and IL-10, in line with the severity of the disease. However, in the second wave, there was no change in iNOS levels with severity, and patients showed lower levels of iNOS, IL-6, and IL-10 compared to the first wave subgroup, suggesting a less severe outcome. In severely ill patients of the second wave, those treated with steroids or azithromycin before hospitalization had significantly lower iNOS levels, indicating a potential impairment in defense against the virus and suggesting NO-based therapies as a potential treatment in patients with low iNOS levels.
Review
Biology
Gaetana Messina, Nicola Tartaglia, Antonio Ambrosi, Chiara Porro, Angelo Campanozzi, Anna Valenzano, Gaetano Corso, Alfonso Fiorelli, Rita Polito, Mario Santini, Marcellino Monda, Domenico Tafuri, Giovanni Messina, Antonietta Messina, Vincenzo Monda
Summary: Lung cancer, as the most lethal cancer, is greatly influenced by lifestyle. Regular physical activity has a significant impact on inhibiting lung cancer progression and improving lung function and symptoms.
Article
Cell Biology
Chiara Volani, Alessandra Pagliaro, Johannes Rainer, Giuseppe Paglia, Benedetta Porro, Ilaria Stadiotti, Luisa Foco, Elisa Cogliati, Adolfo Paolin, Costanza Lagrasta, Caterina Frati, Emilia Corradini, Angela Falco, Theresa Matzinger, Anne Picard, Benedetta Ermon, Silvano Piazza, Marzia De Bortoli, Claudio Tondo, Reginald Philippe, Andrea Medici, Alexandros A. Lavdas, Michael J. F. Blumer, Giulio Pompilio, Elena Sommariva, Peter P. Pramstaller, Jakob Troppmair, Viviana Meraviglia, Alessandra Rossini
Summary: The study demonstrates the crucial role of GCN5 in arrhythmogenic cardiomyopathy, reducing lipid accumulation by modulating cellular redox balance pathways.
JOURNAL OF CELLULAR AND MOLECULAR MEDICINE
(2022)
Article
Physiology
Tobias Duennwald, Giuseppe Paglia, Guenter Weiss, Vanna Denti, Martin Faulhaber, Wolfgang Schobersberger, Henning Wackerhage
Summary: This study aimed to investigate alterations in the blood plasma metabolome in response to high intensity concentric-eccentric leg exercise performed at a simulated altitude. Through metabolomics analysis, significant differences were observed in the metabolome immediately after exercise, and some relative metabolite concentrations remained changed on day 8 after exercise.
FRONTIERS IN PHYSIOLOGY
(2022)
Article
Medicine, General & Internal
Vincenzo Carnovale, Filippo Scialo, Monica Gelzo, Paola Iacotucci, Felice Amato, Federica Zarrilli, Assunta Celardo, Giuseppe Castaldo, Gaetano Corso
Summary: The past decade has witnessed significant advancement in the treatment of Cystic Fibrosis (CF) through the development and combination of CFTR correctors and potentiators. The latest approved triple combination elexacaftor/tezacaftor/ivacaftor has been shown to improve lung function in CF patients and positively impact liver function and nutrient absorption, particularly in those with the Phe508del/minimal function genotype.
JOURNAL OF CLINICAL MEDICINE
(2022)
Article
Biochemistry & Molecular Biology
Chiara Volani, Christa Malfertheiner, Giulia Caprioli, Soren Fjelstrup, Peter P. Pramstaller, Johannes Rainer, Giuseppe Paglia
Summary: Volumetric absorptive microsampling (VAMS) is a minimally invasive sample collection method for blood biomolecules analysis. This study investigated the stability of VAMS samples during short-term storage and compared the metabolome obtained from capillary blood collected from the fingertip to those of plasma and venous blood. The results showed that VAMS samples are stable at room temperature for up to 6 hours and capillary blood provides better coverage of the metabolome compared to plasma.
Review
Infectious Diseases
Gianfranco La Bella, Francesco Salvato, Graziano Antonio Minafra, Irene Francesca Bottalico, Tiziana Rollo, Lucia Barbera, Samantha De Tullio, Gaetano Corso, Sergio Lo Caputo, Rosella De Nittis, Fabio Arena
Summary: In this study, we report a case of aortic endocarditis caused by A. xylosoxidans in a Non-Hodgkin lymphoma patient, which can cause infections in various body districts in patients with underlying diseases. The treatment of A. xylosoxidans infections is complicated by both intrinsic and acquired resistance.
Article
Biochemistry & Molecular Biology
Pierluigi Reveglia, Carmela Paolillo, Antonella Angiolillo, Gabriella Ferretti, Ruggero Angelico, Rossana Sirabella, Gaetano Corso, Carmela Matrone, Alfonso Di Costanzo
Summary: Alzheimer's disease is the most common cause of dementia in the elderly population. It not only affects the brain, but also disturbs the overall metabolism of the body. Analysis of blood samples from patients with AD revealed significant dysregulation of metabolites, including upregulation of glycerophospholipids and ceramide, and downregulation of glutamic acid, other phospholipids, and sphingolipids. Pathway analysis showed that at least five metabolic pathways related to polar compounds were dysregulated in AD patients.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Review
Biochemistry & Molecular Biology
Erica Gianazza, Maura Brioschi, Ada Iezzi, Giuseppe Paglia, Cristina Banfi
Summary: Lipid-lowering therapies are widely used to prevent ASCVD and related mortality. Omics technologies have been employed to study the mechanisms and effects of these drugs, aiming to improve treatment efficacy and safety. Pharmacometabolomics focuses on studying the effects of drugs on metabolic pathways and the variation of treatment response. This review summarizes significant metabolomic studies on lipid-lowering therapies, including statins, fibrates, and novel drugs and nutraceutical approaches. Integrating pharmacometabolomics data with other omics approaches can help understand the biological mechanisms of lipid-lowering drugs and improve treatment efficacy while reducing side effects.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biochemistry & Molecular Biology
Nicoletta Cordani, Daniela Lisini, Valentina Cocce, Giuseppe Paglia, Ramona Meanti, Maria Grazia Cerrito, Pietro Tettamanti, Luca Bonaffini, Francesca Paino, Giulio Alessandri, Angela Marcianti, Aldo Gianni, Chiara Villa, Mario Mauri, Luca Mologni, Antonio Torsello, Augusto Pessina, Marina Elena Cazzaniga
Summary: Triple-negative breast cancer is a highly aggressive disease with a poor prognosis. While paclitaxel is effective, it can cause peripheral neuropathy as a dose-limiting toxicity. Mesenchymal stromal cells loaded with paclitaxel show stronger inhibitory effects on survival, migration, and tumorigenicity in TNBC cell lines compared to free paclitaxel, suggesting a potential new drug delivery strategy.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Meeting Abstract
Cell & Tissue Engineering
Chiara Volani, Viviana Meraviglia, Marzia De Bortoli, Benedetta Ermon, Theresa Matzinger, Elisa Cogliati, Adolfo Paolin, Giulio Pompilio, Johannes Rainer, Giuseppe Paglia, Peter Pramstaller, Elena Sommariva, Alessandra Rossini
TISSUE ENGINEERING PART A
(2022)