4.4 Article

Spatial Dynamics of DNA Damage Response Protein Foci along the Ion Trajectory of High-LET Particles

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RADIATION RESEARCH
卷 176, 期 6, 页码 706-715

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RADIATION RESEARCH SOC
DOI: 10.1667/RR2592.1

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资金

  1. DFG Cluster of Excellence
  2. Munich-Centre for Advanced Photonics
  3. CAS [Y150310BR0]
  4. BfS [StSch 4450]
  5. Maier Leibnitz Laboratorium of the Ludwig-Maximilians-Universitat Munchen
  6. Technische Universitat Munchen

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Du, G., Drexler, G. A., Friedland, W., Greubel, C., Hable, V., Krucken, R., Kugler, A, Tonelli, L., Friedl, A. A. and Dollinger, G. Spatial Dynamics of DNA Damage Response Protein Foci along the Ion Trajectory of High-LET Particles. Radiat. Res. 176, 706-715 (2011). High-linear energy transfer (LET) ion irradiation of cell nuclei induces complex and severe DNA lesions, and foci of repair proteins are formed densely along the ion trajectory. To efficiently discriminate the densely distributed/overlapping foci along the ion trajectory, a focus recognition algorithm called FociPicker3D based on a local fraction thresholding technique was developed. We analyzed high-resolution 3D immunofluorescence microscopic focus images and obtained the kinetics and spatial development of gamma-H2AX, 53BP1 and phospho-NBS1 foci in BJ1-hTERT cells irradiated with 55 MeV carbon ions and compared the results with the dynamics of double-strand break (DSB) distributions simulated using the PARTRAC model. Clusters consisting of several foci were observed along the ion trajectory after irradiation. The spatial dynamics of the protein foci supports that the foci clusters are not formed by neighboring foci but instead originate from the DSB cluster damage induced by high-LET radiations. (C) 2011 by Radiation Research Society

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First direct measurement of 59Cu(p, α) 56Ni: A step towards constraining the Ni-Cu cycle in the cosmos

J. S. Randhawa, R. Kanungo, J. Refsgaard, P. Mohr, T. Ahn, M. Alcorta, C. Andreoiu, S. S. Bhattacharjee, B. Davids, G. Christian, A. A. Chen, R. Coleman, P. E. Garrett, G. F. Grinyer, E. Gyabeng Fuakye, G. Hackman, J. Hollett, R. Jain, K. Kapoor, R. Krucken, A. Laffoley, A. Lennarz, J. Liang, Z. Meisel, B. Nikhil, A. Psaltis, A. Radich, M. Rocchini, N. Saei, M. Saxena, M. Singh, C. Svensson, P. Subramaniam, A. Talebitaher, S. Upadhyayula, C. Waterfield, J. Williams, M. Williams

Summary: Reactions on proton-rich nuclides play a crucial role in nucleosynthesis in core collapse supernovae and x-ray bursts. However, the Ni-Cu cycle may pose a challenge for the production of heavier elements during the Cu-59(p, alpha) Ni-56 reaction. New research suggests that the vp process could operate at higher temperatures, making it a viable pathway for synthesizing heavier elements.

PHYSICAL REVIEW C (2021)

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