Article
Cell & Tissue Engineering
Jianan Jiang, Jinhua Qin, Jisheng Li, Xiaosong Lin, Bowen Zhang, Zeng Fan, Lijuan He, Quan Zeng, Wen Yue, Min Zheng, Xuetao Pei, Yanhua Li
Summary: This study found that the small molecule Ricolinostat can promote the differentiation of megakaryocytic cells from HSPCs, increase the proliferation of megakaryocytic cells, and decrease the secretion of IL-8 and the expression of CXCR2, thereby improving the efficiency of megakaryocytic differentiation of HSPCs.
STEM CELL RESEARCH & THERAPY
(2022)
Article
Urology & Nephrology
Steicy Sobrino, Chrystelle Abdo, Benedicte Neven, Adeline Denis, Nathalie Gouge-Biebuyck, Emmanuel Clave, Soeli Charbonnier, Tifanie Blein, Camille Kergaravat, Marion Alcantara, Patrick Villarese, Romain Berthaud, Laurene Dehoux, Souha Albinni, Esma Karkeni, Chantal Lagresle-Peyrou, Marina Cavazzana, Remi Salomon, Isabelle Andre, Antoine Toubert, Vahid Asnafi, Capucine Picard, Stephane Blanche, Elizabeth Macintyre, Olivia Boyer, Emmanuell Six, Julien Zuber
Summary: Long-term multilineage hematopoietic donor chimerism can occur in patients who receive a transplanted solid organ with lymphoid tissues, such as the intestine or liver. However, there is currently no evidence for kidney-resident hematopoietic stem cells in any mammal species. In this study, we found that human kidney-derived hematopoietic stem cells can reside in the recipient's bone marrow and replace host counterparts, leading to full donor chimerism of both lymphoid and myeloid lineages.
KIDNEY INTERNATIONAL
(2023)
Article
Engineering, Biomedical
Yun Chang, Ramizah Syahirah, Stephanie N. Oprescu, Xuepeng Wang, Juhyung Jung, Scott H. Cooper, Sandra Torregrosa-Allen, Bennett D. Elzey, Alan Y. Hsu, Lauren N. Randolph, Yufei Sun, Shihuan Kuang, Hal E. Broxmeyer, Qing Deng, Xiaojun Lian, Xiaoping Bao
Summary: This study demonstrates the successful generation of AGM-like hematopoietic system from human pluripotent stem cells through temporal manipulation of Wnt signaling. The generated hematopoietic progenitor cells resemble primary cord blood HSCs at the transcriptional level and exhibit lymphoid and myeloid potential in vitro. The cells also show the ability to home to a definitive hematopoietic site, rescue bloodless zebrafish, and engraft and repopulate in mouse recipients.
Article
Cell & Tissue Engineering
Kenji Kitajima, Minako Shingai, Hikaru Ando, Mako Hamasaki, Takahiko Hara
Summary: Researchers developed a novel method for hematopoietic differentiation of human-induced pluripotent stem cells to address the issue of low production of CD38(+) hematopoietic progenitor cells. They found that the lack of FLT3 expression was responsible for this problem and solved it by adding interferon-gamma.
Review
Biochemistry & Molecular Biology
Laura El-Nachef, Joelle Al-Choboq, Juliette Restier-Verlet, Adeline Granzotto, Elise Berthel, Laurene Sonzogni, Melanie L. Ferlazzo, Audrey Bouchet, Pierre Leblond, Patrick Combemale, Stephane Pinson, Michel Bourguignon, Nicolas Foray
Summary: The individual response to ionizing radiation (IR) raises various medical, scientific, and societal concerns. From the 1930s, there has been confusion in the literature regarding the term radiosensitivity, which has been used interchangeably to describe the toxic, cancerous, or aging effects of IR. Differences in mechanisms causing predisposition to radiation-induced adverse tissue reactions (radiosensitivity) and cancer (radiosusceptibility) have been observed, with ATM protein playing a potential role in classifying genetic syndromes associated with radiosensitivity and radiosusceptibility.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Immunology
Shufeng Luo, Huiling Lin, Lan Zhu, Hai-Tian Chen, Siqian Yang, Jinheng Li, Mingyu Liu, Limin Zheng, Chong Wu
Summary: Under stress conditions, HSPCs can interpret danger signals into cytokine signals to modify hematopoietic output. A flow cytometry-based method was developed to assess intracellular cytokines in HSPCs, revealing distinct regulatory modes of cytokine production depending on subset, cytokine, disease, organ, and species. This technical advance helps to better understand HSPC heterogeneity based on differential cytokine production.
FRONTIERS IN IMMUNOLOGY
(2021)
Article
Cell & Tissue Engineering
Zenghua Lin, Maile K. Hollinger, Zhijie Wu, Wanling Sun, Kaylind Batey, Jisoo Kim, Jichun Chen, Xingmin Feng, Neal S. Young
Summary: It was found that sirolimus enhanced the regeneration of hematopoietic stem and progenitor cells in mice exposed to sublethal total body irradiation and other regenerative stressors. The effects were associated with increased expression of hematopoietic genes, reduced DNA damage and clearance of cellular reactive oxygen species. Sirolimus also showed potential clinical applications for the treatment and prevention of hematopoietic injury.
Review
Hematology
Vincenzo Calvanese, Hanna K. A. Mikkola
Summary: Developmental hematopoiesis is a complex process involving the generation of differentiated blood cells and the establishment of a pool of undifferentiated hematopoietic stem cells (HSCs) for postnatal life. Recent single-cell studies have identified rare human HSCs and traced their origin to a unique type of arterial endothelium in the aorta-gonad-mesonephros region. These studies provide new insights into HSC generation and may inform efforts to generate HSCs from pluripotent stem cells in vitro.
Letter
Oncology
Liaoliao Dong, Chuijin Wei, Shumin Xiong, Ping Yu, Ren Zhou, Lin Cheng
Summary: This study demonstrated for the first time that human committed hematopoietic progenitors can be reprogrammed into multipotent progenitors by spliceosome inhibitor. The findings provide a proof-of-concept strategy for generating a large number of HSCs by combining two independent steps.
EXPERIMENTAL HEMATOLOGY & ONCOLOGY
(2022)
Article
Biochemistry & Molecular Biology
You Jung Hwang, Dong-Yeop Shin, Min-Jung Kim, Hyosun Jang, Soyeon Kim, Hyunwon Yang, Won Il Jang, Sunhoo Park, Sehwan Shim, Seung Bum Lee
Summary: This study found that SR1 could alleviate radiation-induced hematopoietic injury. It increased the survival rate and promoted the recovery of peripheral blood cell counts in irradiated mice. In vitro experiments also showed that SR1 increased the population of human hematopoietic stem/progenitor cells, reduced radiation-induced DNA damage and apoptosis. These results suggest that SR1 may be a potential radiomitigator for hematopoietic injury.
Article
Biochemistry & Molecular Biology
Takenobu Nii, Katsuhiro Konno, Masaki Matsumoto, Kanit Bhukhai, Suparerk Borwornpinyo, Kazuhiro Sakai, Suradej Hongeng, Daisuke Sugiyama
Summary: The bioactive peptide SL-13R has been developed to promote the expansion of umbilical cord blood CD34+ cells in vitro, showing significant increases in cell numbers and long-term reconstitution ability. The interaction of SL-13R with specific genes AHNAK, ANXA2, and PLEC suggests potential clinical applications for SL-13R in the future.
Article
Multidisciplinary Sciences
Michele C. Buck, Lisa Bast, Judith S. Hecker, Jennifer Riviere, Maja Rothenberg-Thurley, Luisa Vogel, Dantong Wang, Immanuel Andrae, Fabian J. Theis, Florian Bassermann, Klaus H. Metzeler, Robert A. J. Oostendorp, Carsten Marr, Katharina S. Goetze
Summary: Clonal hematopoiesis of indeterminate potential (CHIP) is the acquisition of somatic mutations in hematopoietic stem/progenitor cells (HSPC) leading to clonal blood cell expansion. Computational modeling shows that alternative hierarchies may better describe HSPC kinetics in CHIP and MDS samples compared to the standard hematopoietic hierarchy. The reorganization of the HSPC compartment can already be detected in the premalignant CHIP state.
Article
Immunology
Lifei Hou, Koichi Yuki
Summary: CD11a plays a critical role in leukocyte arrest and immunological synapse formation, but its role in the bone marrow is not well studied. This research showed that CD11a is expressed on all subsets of hematopoietic stem and progenitor cells (HPSCs). CD11a deficiency enhances HSPCs activity under LPS stimulation, possibly by upregulating the production of IL-27 to promote cell proliferation.
FRONTIERS IN IMMUNOLOGY
(2023)
Article
Engineering, Biomedical
Wenjing Li, Haiwei Liang, Yanxiao Ao, Baixue Tang, Junyang Li, Ning Li, Jianwei Wang, Yanan Du
Summary: Hematopoietic stem cells (HSCs) have the ability to differentiate into all blood cells and immune cells. By simulating the bone marrow microenvironment, we designed degradable scaffolds to regulate the fate of HSCs. In vivo transplantation confirmed the importance of the scaffolds in maintaining hematopoietic function, providing a foundation for parameter design in 3D HSC culture systems.
Article
Immunology
Sabrina B. Bennstein, Sandra Weinhold, Oezer Degistirici, Robert A. J. Oostendorp, Katharina Raba, Gesine Koegler, Roland Meisel, Lutz Walter, Markus Uhrberg
Summary: ILC3, particularly IL-22-secreting ILC3, play vital roles in maintaining mucosal barrier functions and controlling inflammatory diseases. A method for generating functionally competent human ILC3 from cord blood-derived CD34(+) hematopoietic progenitors on human MSCs has been developed, showing promise for potential clinical applications in treating inflammatory diseases and cancer.
FRONTIERS IN IMMUNOLOGY
(2021)
Article
Oncology
Mitsuru Chiba, Narumi Watanabe, Miki Watanabe, Maki Sakamoto, Akika Sato, Mizuki Fujisaki, Shiori Kubota, Satoru Monzen, Atsushi Maruyama, Naoki Nanashima, Ikuo Kashiwakura, Toshiya Nakamura
INTERNATIONAL JOURNAL OF ONCOLOGY
(2016)
Article
Biology
Takakiyo Tsujiguchi, Tokuhisa Hirouchi, Satoru Monzen, Yoshiaki Tabuchi, Ichiro Takasaki, Takashi Kondo, Ikuo Kashiwakura
JOURNAL OF RADIATION RESEARCH
(2016)
Article
Oncology
Satoru Monzen, Mitsuru Chiba, Yoichiro Hosokawa
Article
Oncology
Ryo Saga, Satoru Monzen, Mitsuru Chiba, Hironori Yoshino, Toshiya Nakamura, Yoichiro Hosokawa
Article
Biochemistry & Molecular Biology
Satoru Monzen, Shunta Kimura, Masaru Yamaguchi, Ikuo Kashiwakura
JOURNAL OF INTERFERON AND CYTOKINE RESEARCH
(2018)
Article
Oncology
Satoru Monzen, Mitsuru Chiba, Tatsuya Ueno, Yuki Morino, Kenji Terada, Hiroki Yamaya, Yoichiro Hosokawa
Article
Oncology
Mitsuru Chiba, Shiori Kubota, Ayaka Sakai, Satoru Monzen
MOLECULAR MEDICINE REPORTS
(2018)
Article
Multidisciplinary Sciences
Mitsuru Chiba, Satoru Monzen, Chihiro Iwaya, Yuri Kashiwagi, Sunao Yamada, Yoichiro Hosokawa, Yasushi Mariya, Toshiya Nakamura, Andrzej Wojcik
SCIENTIFIC REPORTS
(2018)
Article
Multidisciplinary Sciences
Mitsuru Chiba, Shiori Kubota, Konomi Sato, Satoru Monzen
SCIENTIFIC REPORTS
(2018)
Article
Oncology
Kosho Murata, Ryo Saga, Satoru Monzen, Echi Tsuruga, Kazuki Hasegawa, Yoichiro Hosokawa
Article
Oncology
Mitsuru Chiba, Haruka Uehara, Ikumi Niiyama, Haruka Kuwata, Satoru Monzen
MOLECULAR MEDICINE REPORTS
(2020)
Article
Oncology
Satoru Monzen, Tatsuya Ueno, Mitsuru Chiba, Yuki Morino, Yasushi Mariya, Andrzej Wojcik, Lovisa Lundholm
Summary: Changes in extracellular microRNAs may serve as biomarkers for radiation-induced damage to human colorectal cancer cells. Specific microRNAs showed a significantly positive correlation with micronuclei frequency in a dose-dependent manner. These findings suggest that these specific microRNAs could be potential markers for cell damage and radiotherapy response in colorectal cancer, but further validation is needed in serum samples from patients undergoing radiotherapy.
MOLECULAR AND CLINICAL ONCOLOGY
(2022)
Article
Oncology
Satoru Monzen, Yota Tatara, Yasushi Mariya, Mitsuru Chiba, Andrzej Wojcik, Lovisa Lundholm
MOLECULAR AND CLINICAL ONCOLOGY
(2020)
Article
Radiology, Nuclear Medicine & Medical Imaging
Yasuyuki Takahashi, Shota Hosokawa, Takakiyo Tsujiguchi, Satoru Monzen, Takao Kanzaki, Koji Shirakawa, Ayaka Nemoto, Hayato Ishimura, Noboru Oriuchi
RADIOLOGICAL PHYSICS AND TECHNOLOGY
(2020)
Article
Medicine, Research & Experimental
Yoichiro Hosokawa, Ryo Saga, Satoru Monzen, Shingo Terashima, Eichi Tsuruga
BIOMEDICAL REPORTS
(2017)