期刊
QUIMICA NOVA
卷 31, 期 3, 页码 704-708出版社
SOC BRASILEIRA QUIMICA
DOI: 10.1590/S0100-40422008000300040
关键词
sequential injection; spectrophotometry; phosphate
The sensitivity and accuracy of sequential injection methods are dependent on efficient overlapping of reagent and sample zones as they are propelled toward the detector cell. The formation of the reduced phosphomolybdic acid is used to demonstrate that the overlapping efficiency in a fixed reaction coil relies on a suitable choice of reagent to sample volume ratio. Additionally, under poor mixing conditions or highly concentrated samples, the reaction extension is strongly dependent on the reagent concentration. The zone-sampling concept is exploited to determine phosphate in cola-based soft drinks after in-line dilution in an auxiliary coil.
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