Article
Chemistry, Analytical
Matthew Humbert, Saiid Lewis, Anthony Borgerding
Summary: The method using a gas-phase microdialysis probe interfaced with a modified commercially available nitric oxide detector can selectively measure aqueous NO at low levels with high selectivity. The quick response time, continuous extraction, and rugged nature of the method make it useful for monitoring NO in biological applications.
Article
Soil Science
Eric W. Slessarev, Aral C. Greene, Peter M. Homyak, Samantha C. Ying, Joshua P. Schimel
Summary: Wetting dry soil leads to emission of nitric oxide (NO), originating from non-biological and biologically-mediated processes. Continuous observations identified two distinct phases of NO production: an initial phase and a prolonged phase, with the former influenced by soil acidity and exchangeable calcium, and the latter associated with nitrite accumulation. These findings provide insights for improving mechanistic models of NO emissions from soils and enhancing predictions of the terrestrial N cycle and atmospheric chemistry.
SOIL BIOLOGY & BIOCHEMISTRY
(2021)
Article
Cardiac & Cardiovascular Systems
Jody L. Greaney, Erika F. H. Saunders, Lacy M. Alexander
Summary: Activation of NF-kappa B, through increased vascular ROS production, contributes to reduced NO-dependent endothelial dilation in young adults with MDD. Short-term treatment with NF-kappa B inhibitor salsalate improves NO-mediated endothelium-dependent dilation in MDD patients, while acute localized ROS scavenging with apocynin enhances NO-dependent dilation before salsalate administration.
AMERICAN JOURNAL OF PHYSIOLOGY-HEART AND CIRCULATORY PHYSIOLOGY
(2022)
Article
Cardiac & Cardiovascular Systems
James T. Miller, Casey G. Turner, Jeffrey S. Otis, Yesser Sebeh, Matthew J. Hayat, Arshed A. Quyyumi, Brett J. Wong
Summary: Our study found that inducible nitric oxide synthase (iNOS) plays a role in the reduced nitric oxide (NO)-dependent vasodilation in prehypertensive individuals and Black participants. Inhibition of iNOS can increase endothelial NO-dependent vasodilation in prehypertensive White participants and in both normotensive and prehypertensive Black participants.
AMERICAN JOURNAL OF PHYSIOLOGY-HEART AND CIRCULATORY PHYSIOLOGY
(2021)
Article
Physiology
Casey G. Turner, Anna E. Stanhewicz, Karen E. Nielsen, Jeffrey S. Otis, Rafaela G. Feresin, Brett J. Wong
Summary: The purpose of this study was to compare endothelial function and NO-dependent vasodilation between women in different hormonal phases and men. The results showed that men had greater endothelium-dependent vasodilation, but lower NO-dependent vasodilation compared to women. Subgroup analysis revealed that women using oral contraceptive pills had significantly greater NO-dependent vasodilation. This study highlights the importance of quantifying NO-dependent vasodilation and provides important implications for experimental design and data interpretation.
JOURNAL OF APPLIED PHYSIOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Rocio Salsoso, Alfonso Mate, Fernando Toledo, Carmen M. Vazquez, Luis Sobrevia
Summary: Late-onset preeclampsia is associated with altered endothelial function in the fetoplacental vasculature, causing an imbalance in the L-arginine/NO signaling pathway and reducing umbilical vein dilation to insulin, which requires A(2B)AR activation in HUVECs.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR BASIS OF DISEASE
(2021)
Article
Neurosciences
E. K. Erickson, A. J. DaCosta, S. C. Mason, Y. A. Blednov, R. D. Mayfield, R. A. Harris
Summary: Astrocytes play a crucial role in the behavioral effects of ethanol through GPCR-mediated calcium signaling. Activation of G(q)-DREADD in astrocytes increases ethanol intake, while reducing calcium signaling decreases sensitivity to ethanol. Astrocyte calcium signaling also alters the stimulatory and sedative-hypnotic effects of ethanol.
NEUROPSYCHOPHARMACOLOGY
(2021)
Article
Biophysics
Jiarong Guo, Tianxiang Wei, Qiongbo Huang, Mingyue Li, Cui Yang, Junhui Mou, Liu Shi, Tao Gao, Genxi Li
Summary: The fabrication of an electrochemical microsensor allows for direct and precise tracing of nitric oxide (NO) release by cells and tissues, providing insights into the dynamics of NO in a tumor microenvironment. This sensor exhibits high sensitivity, wide detection range, and good selectivity, offering a promising technical solution for the bioassay of NO in living systems.
BIOSENSORS & BIOELECTRONICS
(2022)
Article
Neurosciences
Tiago Bortolini, Bruno Melo, Rodrigo Basilio, Ronald Fischer, Roland Zahn, Ricardo de Oliveira-Souza, Brian Knutson, Jorge Moll
Summary: This study explores the intrinsic motivation of humans to bond with others and the role of reward circuitry in the anticipation and response to affiliative rewards. The findings suggest a general appetitive response in the Nucleus Accumbens to different types of rewards, while a more specific response is seen in the septo-hypothalamic region to affiliative rewards. The new task presented in this work allows for distinguishing between neural responses to affiliative and non-affiliative rewards.
Article
Physiology
Casey G. Turner, Matthew J. Hayat, Caroline Grosch, Arshed A. Quyyumi, Jeffrey S. Otis, Brett J. Wong
Summary: Young non-Hispanic Black adults have reduced microvascular endothelial function compared with non-Hispanic White counterparts. The inhibition of endothelin-1 A receptor (ETAR) contributes to the reduced vasodilation in young non-Hispanic Black adults, independent of superoxide production.
JOURNAL OF APPLIED PHYSIOLOGY
(2023)
Article
Clinical Neurology
John J. Alam, Paul Maruff, Susan R. Doctrow, Hui-May Chu, Jennifer Conway, Stephen N. Gomperts, Charlotte Teunissen
Summary: This study evaluates the relationship between plasma tau phosphorylated at residue 181 (ptau181), a biomarker of Alzheimer disease (AD) copathology, and the treatment effects of the p38 alpha kinase inhibitor neflamapimod in patients with dementia with Lewy bodies (DLB). The results suggest that DLB patients with lower pretreatment ptau181 levels may show better treatment response to neflamapimod, with improvement in attention, cognitive assessment, and daily functioning. Plasma biomarkers of AD copathology should be considered as stratification variables in DLB clinical trials.
Article
Biochemistry & Molecular Biology
Yasuo Ito, Harumitsu Nagoya, Masamizu Yamazato, Yoshio Asano, Masahiko Sawada, Tomokazu Shimazu, Makiko Hirayama, Toshimasa Yamamoto, Nobuo Araki
Summary: This study investigated the effects of aging on nitric oxide (NO) production during cerebral ischemia-reperfusion (IR). The results showed that the change in NO3- levels in the plasma of older rats was lower compared to younger rats during cerebral ischemia-reperfusion. Hypertension and aging may be important factors impacting NO production in brain IR injury.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biochemistry & Molecular Biology
Barbara Kutryb-Zajac, Ada Kawecka, Alicja Braczko, Marika Franczak, Ewa M. Slominska, Roberto Giovannoni, Ryszard T. Smolenski
Summary: Chronic hypoxia impairs vascular function through various mechanisms, including changes in mitochondrial respiration. Our study found that the hypoxia-mimetic agent CoCl2 reduces nitric oxide (NO) production, depletes intracellular ATP levels, and increases extracellular nucleotide and adenosine breakdown in endothelial cells (ECs). Supplementation of nucleotide precursors and inhibition of adenosine deaminase can restore ATP levels and protect endothelial function.
Article
Biology
Blaise Robert, Eyal Y. Kimchi, Yurika Watanabe, Tatenda Chakoma, Miao Jing, Yulong Li, Daniel B. Polley
Summary: Research shows that basal forebrain cholinergic neurons have different functional characteristics in different regions, broadcasting diverse modulatory signals to downstream brain regions.
Article
Urology & Nephrology
A. Krishnan, G. Sharma, S. K. Devana, D. Zohmangaihi, R. S. Mavuduru, A. K. Mandal, A. P. Sharma, G. S. Bora
Summary: The study found that patients with underactive bladder have significantly higher levels of urinary NO and decreased levels of ATP. The NO/ATP ratio can be considered as a noninvasive alternative test for diagnosing bladder underactivity, with a high sensitivity, specificity, and diagnostic accuracy.
WORLD JOURNAL OF UROLOGY
(2021)
Article
Clinical Neurology
Vilma Aho, Maija Vainikka, Henri A. J. Puttonen, Heidi M. K. Ikonen, Tiia Salminen, Pertti Panula, Tarja Porkka-Heiskanen, Henna-Kaisa Wigren
JOURNAL OF SLEEP RESEARCH
(2017)
Article
Clinical Neurology
Zoltan Lelkes, Shamsiiat Abdurakhmanova, Tarja Porkka-Heiskanen
JOURNAL OF SLEEP RESEARCH
(2018)
Article
Multidisciplinary Sciences
Katri Kantojarvi, Johanna Liuhanen, Outi Saarenpaa-Heikkila, Anna-Liisa Satomaa, Anneli Kylliainen, Pirjo Polkki, Julia Jaatela, Auli Toivola, Lili Milani, Sari-Leena Himanen, Tarja Porkka-Heiskanen, Juulia Paavonen, Tiina Paunio
Article
Clinical Neurology
Wessel M. A. van Leeuwen, Mikael Sallinen, Jussi Virkkala, Harri Lindholm, Ari Hirvonen, Christer Hublin, Tarja Porkka-Heiskanen, Mikko Harma
SLEEP AND BIOLOGICAL RHYTHMS
(2018)
Review
Physiology
H. -K. Wigren, T. Porkka-Heiskanen
Article
Clinical Neurology
Isabel Morales-Munoz, Outi Saarenpaa-Heikkila, Anneli Kylliainen, Pirjo Polkki, Tarja Porkka-Heiskanen, Tiina Paunio, E. Juulia Paavonen
JOURNAL OF SLEEP RESEARCH
(2018)
Article
Clinical Neurology
Isabel Morales-Munoz, Timo Partonen, Outi Saarenpaa-Heikkila, Anneli Kylliainen, Pirjo Polkki, Tarja Porkka-Heiskanen, Tiina Paunio, E. Juulia Paavonen
Article
Clinical Neurology
E. Juulia Paavonen, Isabel Morales-Munoz, Pirjo Polkki, Tiina Paunio, Tarja Porkka-Heiskanen, Anneli Kylliainen, Timo Partonen, Outi Saarenpaa-Heikkila
JOURNAL OF SLEEP RESEARCH
(2020)
Article
Clinical Neurology
Sonja Sulkava, Antti-Mathias Taka, Katri Kantojarvi, Pirjo Polkki, Isabel Morales-Munoz, Lili Milani, Tarja Porkka-Heiskanen, Outi Saarenpaa-Heikkila, Anneli Kylliainen, E. Juulia Paavonen, Tiina Paunio
JOURNAL OF SLEEP RESEARCH
(2020)
Article
Multidisciplinary Sciences
Antti-Jussi Ammala, Emma I. K. Vitikainen, Iiris Hovatta, Juulia Paavonen, Outi Saarenpaa-Heikkila, Anneli Kylliainen, Pirjo Polkki, Tarja Porkka-Heiskanen, Tiina Paunio
SCIENTIFIC REPORTS
(2020)
Article
Peripheral Vascular Disease
Juliane Hannemann, Anika Laing, Benita Middleton, Jonathan Cridland, Bart Staels, Nikolaus Marx, Peter J. Grant, Massimo Federici, Tarja Stenberg, Debra J. Skene, Rainer Boeger
Summary: The study found that timed light therapy for rotating night shift workers can improve their diurnal blood pressure control and glucose tolerance, increasing the proportion of dippers and reducing catecholamine levels.
JOURNAL OF HYPERTENSION
(2021)
Article
Multidisciplinary Sciences
Alexandra Lahtinen, Antti Hakkinen, Sampsa Puttonen, Paivi Vanttola, Katriina Viitasalo, Tarja Porkka-Heiskanen, Mikko Harma, Tiina Paunio
Summary: The study revealed that DNA methylation in shift work disorder (SWD) is significantly affected by vacation, with a higher percentage of differentially methylated positions (DMPs) compared to controls. The majority of DMPs in SWD showed hypomethylation during the work period, which may be related to the recovery process from circadian disorders.
SCIENTIFIC REPORTS
(2021)
Article
Developmental Biology
Anna Kirjavainen, Parul Singh, Laura Lahti, Patricia Seja, Zoltan Lelkes, Aki Makkonen, Sami Kilpinen, Yuichi Ono, Marjo Salminen, Teemu Aitta-Aho, Tarja Stenberg, Svetlana Molchanova, Kaia Achim, Juha Partanen
Summary: The transcription factor Gata2 plays a critical role in the development of GABAergic neurons in the midbrain, and its downstream genes Nkx2-2 and Skor2 are involved in the diversification of GABAergic neuron subtypes in the midbrain reticular formation (MRF). The Nkx2-2 and Skor2-expressing GABAergic neurons in the MRF are located at the boundary of the ventrolateral periaqueductal gray and the MRF, and are involved in regulating REM sleep. These neurons show increased activity upon REM sleep inhibition and project to the dorsolateral pons, a region associated with sleep control.
Article
Neurosciences
Sarah Katharina Steffens, Tarja Helena Stenberg, Henna-Kaisa Margareta Wigren
Summary: This study investigated the relationship between microglial morphology and the sleep-wake cycle. The results showed that the decrease in microglial features was most prominent within the early hours of the light period, co-occurring with maximal sleep SWA. Throughout the 24-hour cycle, there were minor regional differences in microglial morphology between different brain areas.
Review
Physiology
Tarja Porkka-Heiskanen, Henna-Kaisa Wigren
CURRENT OPINION IN PHYSIOLOGY
(2020)