Article
Engineering, Electrical & Electronic
Panchun Gu, Zengxin Huang, Mi Ping, Wenshuang Li, Meng Xiang, Xizeng Feng, Dengfeng Kuang
Summary: This study presents a novel method to generate multiplane parallel light sheets with high resolution and low phototoxicity using an isosceles triangular array microstructure. Experimental validation and comparison with simulations were performed to evaluate the imaging quality, showing promising results for volumetric imaging in light sheet fluorescence microscopy.
IEEE JOURNAL OF SELECTED TOPICS IN QUANTUM ELECTRONICS
(2021)
Article
Chemistry, Analytical
Taichu Shi, Yang Qi, Cheng Zhu, Ying Tang, Ben Wu
Summary: This paper presents a three-dimensional microscopic system based on structured light illumination for real-time 3D imaging. The system has high resolution and working distance, making it suitable for applications such as bridge and road crack examinations.
Article
Chemistry, Multidisciplinary
Baolei Liu, Jiayan Liao, Yiliao Song, Chaohao Chen, Lei Ding, Jie Lu, Jiajia Zhou, Fan Wang
Summary: The study introduces a method for multiplexed super-resolution imaging using lifetime-engineered upconversion nanoparticles, achieving a lateral resolution of 185 nm. By utilizing a deep learning algorithm, more accurate decoding compared to a numeric algorithm is achieved, with decoding accuracies above 93% for a three-channel super-resolution imaging.
NANOSCALE ADVANCES
(2021)
Article
Biochemical Research Methods
Bingying Chen, Bo-Jui Chang, Philippe Roudot, Felix Zhou, Etai Sapoznik, Madeleine Marlar-Pavey, James B. Hayes, Peter T. Brown, Chih-Wei Zeng, Talley Lambert, Jonathan R. Friedman, Chun-Li Zhang, Dylan T. Burnette, Douglas P. Shepherd, Kevin M. Dean, Reto P. Fiolka
Summary: Structured illumination microscopy (SIM) improves the spatial resolution of fluorescence microscopy, while light-sheet fluorescence microscopy (LSFM) avoids exciting out-of-focus fluorescence. By combining SIM with LSFM using oblique plane microscopy, high-resolution three-dimensional imaging with low phototoxicity can be achieved.
Article
Biochemical Research Methods
Petra Paie, Gianmaria Calisesi, Alessia Candeo, Andrea Comi, Federico Sala, Francesco Ceccarelli, Ada De Luigi, Pietro Veglianese, Korbinian Muhlberger, Michael Fokine, Gianluca Valentini, Roberto Osellame, Mark Neil, Andrea Bassi, Francesca Bragheri
Summary: Heterogeneity investigation at the single-cell level is crucial in clinical research. The combination of light sheet fluorescence microscopy and structured illumination microscopy in an optofluidic platform enables high throughput super-resolution imaging, allowing comprehensive evaluation of cellular heterogeneity at high resolution.
Article
Optics
Zhiqiang Fu, Qiang Geng, Jialong Chen, Li-An Chu, Ann-Shyn Chiang, Shih-Chi Chen
Summary: This study introduces a light sheet-based structured light illumination light field microscopy system for fast 3D imaging, utilizing a digital micromirror device to generate sinusoidal patterns rapidly. By combining deconvolution and HiLo algorithms, fast volumetric imaging with improved optical cross-sectioning capability is achieved. Imaging experiments on various samples show that this new method presents a fast 3D imaging solution with significantly enhanced optical cross-sectioning capability compared to standard light sheet-based LFM.
Article
Nanoscience & Nanotechnology
Ruslan Rohrich, A. Femius Koenderink
Summary: This article introduces a new structured illumination microscopy technique that extends the capabilities of SIM by combining ideas from illumination engineering and nanophotonics. Experimental results demonstrate the potential for resolution enhancements beyond regular SIM, validated through simulations and experiments. However, there are still obstacles to overcome in the implementation of experiments for artifact-free super-resolution imaging.
Article
Optics
Tristan C. Paul, Guy Hagen
Summary: A new technique called Spinning Disk Structured Illumination Microscopy (SD-SIM) was investigated, which combines Spinning Disk microscopy with Structured Illumination Microscopy to achieve better background removal and detect cellular structures that were previously undetectable with either method alone.
Article
Optics
Krishnendu Samanta, Anupriya Tiwari, Shereena Joseph, Joby Joseph
Summary: We propose a novel super-resolution technique called saturable absorption assisted nonlinear structured illumination microscopy (SAN-SIM), which exploits the saturable absorption property of a material. By converting the incident sinusoidal excitation into nonlinear illumination through a saturable absorbing material, the technique achieves more than two-fold resolution improvement over the diffraction limit by generating higher harmonics that fold high frequency components within the passband.
Article
Nanoscience & Nanotechnology
Piotr Zdankowski, Lucia F. Lopez, Guillermo P. Acuna, Fernando D. Stefani
Summary: The localization of single fluorescent emitters is crucial for nanoscale physicochemical and biophysical measurements. MINFLUX and other related methods have achieved breakthroughs in resolution, enabling true nanoscale fluorescence nanoscopy and single-molecule tracking.
Article
Optics
Yile Sun, Hongfei Zhu, Lu Yin, Hanmeng Wu, Mingxuan Cai, Weiyun Sun, Yueshu Xu, Xinxun Yang, Jiaxiao Han, Wenjie Liu, Yubing Han, Xiang Hao, Renjie Zhou, Cuifang Kuang, Xu Liu
Summary: Fluorescence interference structured illumination microscopy (FI-SIM) is a new imaging modality that allows for high axial resolution imaging without the need for z-axis scanning. It can quickly acquire images with relatively low laser power density, making it suitable for observing fixed and live subcellular structures.
ADVANCED PHOTONICS
(2023)
Article
Optics
Sibi chakravarthy s Hanmugavel, Yunhuiz Hu
Summary: We present a technique for sub-diffraction resolution imaging of non-fluorescent samples through quantitative phase imaging. By applying structured illumination microscopy (SIM) in a novel way, we are able to extract high spatial frequency components from defocused intensity images and reconstruct quantitative phase images with a frequency spectrum that exceeds the diffraction limit imposed by the imaging system. This approach offers several advantages, including a deterministic, phase-unwrapping-free algorithm and a noninterferometric setup that is easy to implement.
Article
Optics
Liming Chen, Song Zhang
Summary: This paper proposes a method to drastically increase the depth of field (DOF) of microscopic 3D imaging by leveraging the focus stacking technique and developing a novel computational framework. Experimental results demonstrate that the proposed method can achieve a large DOF of approximately 2 mm and a field of view (FOV) of approximately 4 mm x 3 mm.
OPTICS AND LASERS IN ENGINEERING
(2023)
Article
Optics
Yanhong Gan, Zitong Ye, Yubing Han, Ye Ma, Chuankang Li, Qiulan Liu, Wenjie Liu, Cuifang Kuang, Xu Liu
Summary: Light sheet fluorescence microscopy (LSFM) is a promising tool for biological research due to its ability to observe living cells dynamically. However, obtaining optimal image quality in LSFM requires precise alignment between the light sheet and detection focal plane. In this study, we propose a fast and accurate autofocusing method based on deep learning to overcome the challenge of unstable focusing in LSFM with a single shot. Our method is compatible with any light sheet imaging setup using a spatial light modulator. It achieves a predicted root-mean-square error of 0.0942 μm within a range of ± 0.7 μm in a light sheet microscope with a 1.1 numerical aperture detection objective. The neural network architecture we propose has the advantages of small memory size, few training data requirements, and good generalization to untrained sample types.
OPTICS AND LASERS IN ENGINEERING
(2023)
Article
Biotechnology & Applied Microbiology
Xuesong Li, Yicong Wu, Yijun Su, Ivan Rey-Suarez, Claudia Matthaeus, Taylor B. B. Updegrove, Zhuang Wei, Lixia Zhang, Hideki Sasaki, Yue Li, Min Guo, John P. P. Giannini, Harshad D. D. Vishwasrao, Jiji Chen, Shih-Jong J. Lee, Lin Shao, Huafeng Liu, Kumaran S. S. Ramamurthi, Justin W. W. Taraska, Arpita Upadhyaya, Patrick La Riviere, Hari Shroff
Summary: Enhanced axial resolution for 3D SIM is achieved with deep learning or four-beam interference. These methods allow for higher spatial frequency content and near-isotropic imaging with resolutions of around 120 nm in both lateral and axial directions. The deep learning method can also be combined with denoising for volumetric imaging. Various cellular samples were imaged to demonstrate the potential of these advancements.
NATURE BIOTECHNOLOGY
(2023)
Article
Hematology
Nadia Brunacci, Christian Wischke, Toufik Naolou, Alexa Patzelt, Juergen Lademann, Axel T. Neffe, Andreas Lendlein
Summary: This study focused on the fabrication of oligodepsipeptides nanoparticulate carrier systems, demonstrating the feasibility of drugloading and size control of OBMD submicron particles. The results showed that the particles could enable drug penetration into hair follicles with sustained release profile, indicating their potential advantages over cream formulations.
CLINICAL HEMORHEOLOGY AND MICROCIRCULATION
(2021)
Correction
Biochemistry & Molecular Biology
Pin Dong, Christian Teutloff, Jurgen Lademann, Alexa Patzelt, Monika Schafer-Korting, Martina C. Meinke
CELL BIOCHEMISTRY AND BIOPHYSICS
(2022)
Article
Dermatology
Dimitra Aikaterini Lintzeri, Andria Constantinou, Kathrin Hillmann, Kamran Ghoreschi, Annika Vogt, Ulrike Blume-Peytavi
Summary: Alopecia areata is a chronic immune-mediated disease with diverse clinical manifestations. This article discusses the current diagnostic and therapeutic options, as well as emerging treatments. The psychosocial impact of the disease on patients' quality of life is significant and should be addressed with appropriate psychological support.
JOURNAL DER DEUTSCHEN DERMATOLOGISCHEN GESELLSCHAFT
(2022)
Review
Dermatology
Maxim E. Darvin, Johannes Schleusener, Juergen Lademann, Chun-Sik Choe
Summary: Confocal Raman microspectroscopy is commonly used for noninvasive analysis of skin components and physiological parameters of the stratum corneum, and this paper summarizes recent results in this field.
SKIN PHARMACOLOGY AND PHYSIOLOGY
(2022)
Article
Chemistry, Applied
Victor H. P. Infante, Patricia M. B. G. Maia Campos, Lorena Rigo Gaspar, Maxim E. Darvin, Johannes Schleusener, Karen C. Rangel, Martina C. Meinke, Juergen Lademann
Summary: This study evaluated the safety and synergistic effects of tea tree, lavender, eucalyptus, and tangerine essential oils on the skin. The results showed that these essential oils, when used in combination and at appropriate doses, were safe and effective in improving the hydrolipidic balance and morphological properties of the skin. No adverse effects were reported in the clinical study.
INTERNATIONAL JOURNAL OF COSMETIC SCIENCE
(2022)
Article
Dermatology
Ulrike Blume-Peytavi, Kathrin Hillmann, Andria Constantinou, Annika Vogt
Summary: The number of patients with frontal fibrosing alopecia (FAA) is increasing, and it is important to recognize the clinical pattern and symptoms for proper management and treatment. Experimental studies and case series shed light on the pathogenesis, possible triggers, clinical course, and treatment options of FAA. The clinical spectrum of FAA extends beyond the typical recession of the frontal hairline in postmenopausal women and can also affect younger women, men, and rarely adolescents.
Article
Spectroscopy
V. S. Novikov, V. V. Kuzmin, M. E. Darvin, J. Lademann, E. A. Sagitova, K. A. Prokhorov, L. Yu Ustynyuk, G. Yu Nikolaeva
Summary: Using density functional theory (DFT), the study analyzed the structures and Raman spectra of trans-isomers of different carotenoids, finding that the position of the C=C stretching band is mainly determined by the number of conjugated C=C bonds and decreases with increased conjugation length. The C-C stretching band shifts with conjugation length or mass of end groups, and the presence of side CH3 groups affects the intensity and position of the bands.
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
(2022)
Article
Biochemistry & Molecular Biology
Victor Hugo Infante, Patricia Maria Maia Campos, Maxim Darvin, Silke Lohan, Johannes Schleusener, Sabine Schanzer, Juergen Lademann, Martina Meinke
Summary: This study aimed to evaluate the penetration depth, antioxidant capacity, and clinical efficacy of Melaleuca alternifolia pure essential oil and its nanoemulsion in preventing skin photoaging. The results showed that the nanoemulsion had a higher penetration through the stratum corneum, improving skin morphology and exhibiting potential for treating photoaged skin.
PHOTOCHEMISTRY AND PHOTOBIOLOGY
(2023)
Article
Biochemical Research Methods
ChunSik Choe, Johannes Schleusener, JinSong Ri, SeHyok Choe, PokSil Kim, Juergen Lademann, Maxim E. Darvin
Summary: In this study, confocal Raman micro-spectroscopy and tMCR-ALS method were used to quantitatively determine the concentrations of water, lipids, proteins, and topically applied oils in the stratum corneum (SC) by analyzing Raman spectra in the 2000-4000 cm(-1) region.
JOURNAL OF BIOPHOTONICS
(2023)
Article
Biology
Marius Kroger, Jorg Scheffel, Evgeny A. Shirshin, Johannes Schleusener, Martina C. Meinke, Jurgen Lademann, Marcus Maurer, Maxim E. Darvin
Summary: This study demonstrates a noninvasive method (TPE-FLIM) that can visualize macrophages in human dermis. The method shows high sensitivity and specificity, making it valuable for studying the role of macrophages in health and disease.
Article
Pharmacology & Pharmacy
Mohammad Alhibah, Marius Kroeger, Sabine Schanzer, Loris Busch, Juergen Lademann, Ingeborg Beckers, Martina C. Meinke, Maxim E. Darvin
Summary: Understanding the penetration depth and pathways of drugs and cosmetics into the skin is important for drug delivery and cosmetic safety. In this study, TPT-FLIM was used to visualize the skin structure and study the penetration depths of different substances. The results showed that the fluorescence lifetime is the most appropriate parameter for building penetration profiles.
Article
Biochemistry & Molecular Biology
Alexandra Lan, Yan Lui, Jinhui Zuo, Silke B. B. Lohan, Sabine Schanzer, Sabrina Wiemann, Cornelia M. M. Keck, Jurgen Lademann, Martina C. C. Meinke
Summary: Antioxidants can reduce free radical formation in deeper skin layers where typical sunscreen filters may no longer be effective. Here, a general method is presented to pre-select optimum combinations of antioxidants and physical filters.
Article
Immunology
F. Rancan, K. Rajes, P. Sidiropoulou, S. Hadam, X. Guo, F. Zabihi, U. Mirastschijski, E. Ruehl, R. Haag, U. Blume-Peytavi, A. Vogt
Summary: Rapamycin, a potential anti-proliferative drug, has limitations in topical treatment of skin disorders due to its high molecular weight and lipophilicity. This study demonstrated that oxidation-sensitive CMS nanocarriers improved drug delivery to the skin and showed mTOR inhibitory activity in an inflammatory ex vivo human skin model. The osCMS formulations achieved higher anti-inflammatory effects and downregulated mTOR activity, suggesting their potential use in topical anti-inflammatory therapy.
INTERNATIONAL IMMUNOPHARMACOLOGY
(2023)
Article
Biochemical Research Methods
Kerstin H. Gehlich, Georges Koch, Wolfgang Koecher, Martina C. Meinke, Bernhard Lange-Asschenfeldt, Juergen Lademann
Summary: Antioxidants play a vital role in defending against aging and chronic disease. The concentration of cutaneous carotenoids in the skin serves as a biomarker for antioxidant intake, and biofeedback can effectively improve cutaneous carotenoid levels. A study involving 44 adults found that providing biofeedback measurements, along with additional fruit juice intake, led to increased carotenoid levels over time. Therefore, biofeedback may offer a cost-effective method for monitoring and improving individual antioxidant status.
JOURNAL OF BIOPHOTONICS
(2023)
Article
Dermatology
Marius Kroeger, Johannes Schleusener, Juergen Lademann, Martina C. C. Meinke, Sora Jung, Maxim E. E. Darvin
Summary: The study used two-photon excited fluorescence lifetime imaging (TPE-FLIM) to investigate the localization and distribution of tattoo ink pigments in freshly and old tattooed human skin. The results showed that carbon black particles were still visible after 84 days of tattoo application, indicating delayed recovery of the epidermis. Carbon black tattoo pigments were found in various skin components in both freshly applied and old tattoos.
Evaluation: 8/10
