期刊
PROTOPLASMA
卷 251, 期 2, 页码 333-347出版社
SPRINGER WIEN
DOI: 10.1007/s00709-013-0590-z
关键词
FRET biosensors; Fluorescent protein; Second messengers; Plants; Mammalian cells; Biosensor design
资金
- NanoNextNL, a micro- and nanotechnology consortium of the Government of The Netherlands
- Netherlands Organisation for Scientific Research (NWO)
Genetically encoded biosensors are increasingly used in visualising signalling processes in different organisms. Sensors based on green fluorescent protein technology are providing a great opportunity for using Forster resonance energy transfer (FRET) as a tool that allows for monitoring dynamic processes in living cells. The development of these FRET biosensors requires careful selection of fluorophores, substrates and recognition domains. In this review, we will discuss recent developments, strategies to create and optimise FRET biosensors and applications of FRET-based biosensors for use in the two major eukaryotic kingdoms and elaborate on different methods for FRET detection.
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