Review
Biochemistry & Molecular Biology
Leia Colin, Raquel Martin-Arevalillo, Simone Bovio, Amelie Bauer, Teva Vernoux, Marie-Cecile Caillaud, Benoit Landrein, Yvon Jaillais
Summary: Biological imaging is an interdisciplinary field crucial in cell biology research, involving techniques, probe development, and image analysis. Recent advances in live imaging of plant cells include studies on cell structure, hormones, mechanical properties, etc. driving the field forward.
Review
Plant Sciences
Camila Goldy, Marie-Cecile Caillaud
Summary: Plants have developed cellular mechanisms to respond to signals by rearranging the cytoskeleton, regulated by phospholipids such as phosphatidic acid and phosphoinositides. This review focuses on the emerging role of phosphatidylinositol 4,5-bisphosphate in regulating the peripheral cytoskeleton during cellular processes.
CURRENT OPINION IN PLANT BIOLOGY
(2023)
Editorial Material
Biochemistry & Molecular Biology
Michael J. Deeks
Summary: The study highlights the importance of microbe-plant cell interactions in shaping plant cell morphology and function, particularly demonstrating how a specialized formin protein facilitates nitrogen-fixing bacteria colonization in legumes.
Article
Chemistry, Analytical
Qiaomei Yang, Yizhuang Zhou, Libin Tan, Can Xie, Xiaowen Li, Miao Zeng, Liyi Zhou
Summary: For the early diagnosis and effective evaluation of treatment effects of inflammation, a de novo bioanalytical method is urgently needed to monitor the metabolite nitric oxide (NO) associated with inflammatory diseases. In this work, a de novo host-guest self-assembled nanosensor CTA was reported for the quantitative detection and visualization of NO levels in inflammatory models.
ANALYTICAL CHEMISTRY
(2023)
Review
Plant Sciences
Noriyoshi Yagi, Akira Yoshinari, Ryu J. Iwatate, Reika Isoda, Wolf B. Frommer, Masayoshi Nakamura
Summary: Fluorescent probes are essential tools for visualizing cellular structures and molecular dynamics in living cells, with a wide variety of small-molecule dyes synthesized for labeling purposes. Self-labeling technologies allow these probes to be attached to cellular domains or specific proteins, and are now being increasingly utilized in plant research.
PLANT AND CELL PHYSIOLOGY
(2021)
Article
Chemistry, Analytical
Yukun Zhang, Chen Xu, Hui Sun, Jindong Ai, Mingguang Ren
Summary: Hydrazine (N2H4) is an important industrial substance that is highly toxic and can cause severe environmental pollution and harm to human beings. A new water-soluble fluorescent probe based on coumarin fluorophore, C-Z1, has been developed for the simple and effective detection of N2H4 in environmental systems and organisms. C-Z1 shows good selectivity and sensitivity with a detection limit of hydrazine hydrate at 1.48 x 10-7 M, and it has been successfully used to detect N2H4 in various environmental samples and can be used for rapid quantitative detection in the field.
Article
Biochemistry & Molecular Biology
Neus Gacias-Amengual, Lena Wohlschlager, Florian Csarman, Roland Ludwig
Summary: In this study, lignocellulosic enzymes from the white rot fungus Phanerochaete chrysosporium were localized on model substrates and poplar wood using confocal laser scanning microscopy. Two detection approaches were investigated, revealing the innermost secondary cell wall layer as the preferential attack point for cellulose-degrading enzymes. The methods presented are suitable for visualizing enzymes during catalytic biomass degradation and can be further utilized for interaction studies in biorefineries.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Multidisciplinary Sciences
Jonas Bucevicius, Ruta Gerasimaite, Kamila A. A. Kiszka, Shalini Pradhan, Georgij Kostiuk, Tanja Koenen, Grazvydas Lukinavicius
Summary: The authors present a convenient protecting-group-free synthesis method for generating a wide range of symmetrical and unsymmetrical 4-carboxyrhodamines that cover the entire visible spectrum. This approach significantly reduces the number of synthesis steps, expands the structural diversity, and allows for gram-scale synthesis of the dyes. The availability of suitable fluorescent probes is crucial for the development of live-cell fluorescence nanoscopy.
NATURE COMMUNICATIONS
(2023)
Review
Cell Biology
Weida Ren, Wanyu Zhao, Lingbo Cao, Junqi Huang
Summary: Research on programmed cell death (PCD) reveals that cells undergo orderly cell death through evolutionary regulatory mechanisms, with actin playing a crucial role in cellular processes. Scientists aim to further understand the intricate relationship between PCD and the actin cytoskeleton.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Review
Chemistry, Analytical
Zheng Wang, Ji Ma, Changlin Li, Haichang Zhang
Summary: Fluorescent conjugated materials commonly exhibit weak or quenching luminescent phenomena in the solid or aggregate state, limiting their applications in medicine and biology. However, aggregation-induced emission (AIE) fluorescent materials have shown strong luminescent properties in the aggregate state, overcoming the ACQ phenomenon. These AIE materials have been successfully used in biolabeling to detect ions and monitor physiological activity, making them popular biofluorescent probe materials.
Review
Plant Sciences
Jingze Zang, Verena Kriechbaumer, Pengwei Wang
Summary: Plant endoplasmic reticulum (ER) remodelling is important for targeted protein secretion, organelle interaction, and signal exchange, regulated by actin cytoskeleton and microtubule-based machinery. The dependence on cell type and developmental stages for ER dynamics in plants is highlighted in recent discoveries. The ER membrane also plays a role in organizing the cytoskeletal network, impacting cytoskeleton structure.
JOURNAL OF PLANT PHYSIOLOGY
(2021)
Review
Plant Sciences
Sunil Kumar, Theboral Jeevaraj, Mohd H. Yunus, Subhra Chakraborty, Niranjan Chakraborty
Summary: This review summarizes recent advances in the role of plant cytoskeleton and associated proteins in abiotic stress management. Plants have evolved specific mechanisms to sense external stress and transmit information for appropriate responses. The plant cytoskeleton, along with associated proteins, not only plays a role in cell growth and proliferation but also functions in stress response and resilience.
PLANT CELL AND ENVIRONMENT
(2023)
Review
Chemistry, Multidisciplinary
Shahi Imam Reja, Masafumi Minoshima, Yuichiro Hori, Kazuya Kikuchi
Summary: The development of near-infrared fluorescent probes has revolutionized biomolecular imaging and is now used as an important tool in understanding intracellular activities. The coupling of self-labeling protein tags with synthetic fluorescent probes is becoming a significant research area in chemical biology.
Article
Chemistry, Analytical
Mahadeva M. M. Swamy, Mohamad Zarif Mohd Zubir, Mutmainah, Setsuko Tsuboi, Yuta Murai, Kenji Monde, Ken-ichi Hirano, Takashi Jin
Summary: In this study, a near-infrared fluorescent labeled long-chain fatty acid (LCFA) was synthesized for real-time imaging of cardiac metabolism in living mice. The uptake of the fluorescent LCFA analogue was observed through in vivo and ex vivo near-infrared fluorescence imaging, showing significant differences between fasted and fed mice.
Article
Chemistry, Analytical
Li Xu, Haiyang Xie, Boqian Wang, Zijian Zhu, Hui Jiang, Xiaoqian Duan, Shuxin Deng, Jiasu Xu, Lai Jiang, Xianting Ding
Summary: Single-cellwestern blotting (scWB) is a common method for high-resolution protein analysis on low-abundance cell samples. However, antibody stripping during repeated staining leads to signal loss, preventing multiplex protein detection. In this study, we introduce Fluorescent-quenching Aptamer-based Single-cell Western Blotting (FAS-WB) for multiplex protein detection at single-cell resolution. FAS-WB allows rapid penetration, diffusion, and elution of aptamer probes, while avoiding signal loss through the use of fluorophore-tagged aptamers coordinated with quenching strands.
ANALYTICAL CHEMISTRY
(2023)
