期刊
PROTIST
卷 159, 期 1, 页码 115-125出版社
ELSEVIER GMBH
DOI: 10.1016/j.protis.2007.09.001
关键词
C2 domain; Kinetoplastida; microtubules; plasma membrane; T. brucei; trypanosomes
类别
资金
- Wellcome Trust Funding Source: Medline
WCB is a protein that locates between the inner face of the plasma membrane and the sub-pellicular corset of microtubules in Trypanosoma brucei. We provide the molecular identity of WCB and bioinformatic analysis suggests that it possesses a C2 domain implicated in membrane/protein interactions and a highly charged region possessing characteristics of a putative tubulin-binding domain. Functional analyses via RNA interference (RNAi) depletion show that WCB is essential for cell morphogenesis. Depletion results in gross abnormalities in cell shape, mainly at the cytoskeletal/plasma membrane dynamic posterior end of the trypanosome. Failures in cytokinesis and zoid production are also evident. Furthermore, electron microscopy reveals that RNAi-induced trypanosomes lose local plasma membrane to microtubule corset integrity. (c) 2007 Elsevier GmbH. All rights reserved.
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