期刊
PROTEOMICS CLINICAL APPLICATIONS
卷 8, 期 7-8, 页码 620-625出版社
WILEY-V C H VERLAG GMBH
DOI: 10.1002/prca.201400010
关键词
Abdominal aortic aneurysm; Microvesicles
资金
- EC
- FAD project (FP-7) [HEALTH F2-2008-200647]
- Spanish MICIN [SAF2013/42525]
- Ministerio de Sanidad y Consumo, Instituto de Salud Carlos III, Redes RIC, biobancos [RD12/0042/00038, RD09/0076/00101]
- Fundacion Conchita Rabago
- Region Midi-Pyrenees
- European funds (FEDER, Fonds europeens de developpement regional)
- French Ministry of Research (Investissements d'avenir, Proteomics French Infrastructure)
Purpose: To find potential biomarkers of abdominal aortic aneurysms (AAA), we performed a differential proteomic study based on human plasma-derived microvesicles. Experimental design: Exosomes and microparticles isolated from plasma of AAA patients and control subjects (n = 10 each group) were analyzed by a label-free quantitative MS-based strategy. Homemade and publicly available software packages have been used for MS data analysis. Results: The application of two kinds of bioinformatic tools allowed us to find differential protein profiles from AAA patients. Some of these proteins found by the two analysis methods belong to main pathologicalmechanisms of AAA such as oxidative stress, immune-inflammation, and thrombosis. Conclusions and clinical relevance: Data analysis from label-free MS-based experiments requires the use of sophisticated bioinformatic approaches to perform quantitative studies from complex protein mixtures. The application of two of these bioinformatic tools provided us a preliminary list of differential proteins found in plasma-derived microvesicles not previously associated to AAA, which could help us to understand the pathological mechanisms related to this disease.
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