Article
Multidisciplinary Sciences
Milene C. Vallejo, Soumyadeep Sarkar, Emily C. Elliott, Hayden R. Henry, Samantha M. Powell, Ivo Diaz Ludovico, Youngki You, Fei Huang, Samuel H. Payne, Sasanka Ramanadham, Emily K. Sims, Thomas O. Metz, Raghavendra G. Mirmira, Ernesto S. Nakayasu
Summary: Extracellular vesicles (EVs) have important roles in cell-to-cell communication and biomarker studies. In this study, a proteomics meta-analysis was performed to refine the composition of plasma EVs by separating EV proteins and contaminants into different clusters. The refined EV protein list obtained from this study provides a valuable resource for mechanistic and biomarker studies.
Article
Biology
Wouter W. Woud, Edwin van der Pol, Erik Mul, Martin J. Hoogduijn, Carla C. Baan, Karin Boer, Ana Merino
Summary: This article presents a method to quantify and phenotype extracellular vesicles (EVs) in human plasma using imaging flow cytometry without prior isolation of EVs. The method eliminates selection bias or artifact introduced by EV isolation techniques and provides a framework for analyzing EVs in various experimental and potentially diagnostic settings.
COMMUNICATIONS BIOLOGY
(2022)
Article
Biology
Sujan Kumar Mondal, Derick Haas, Jie Han, Theresa L. L. Whiteside
Summary: Small extracellular vesicles (sEV) in TNBC patients' plasma promote T cell dysfunction and tumor progression. Tumor cell-derived exosomes (TEX) carrying surface proteins induce apoptosis of CD8(+)T and CD4(+)T cells but spare B and NK cells. TEX entry into T cells induces mitochondrial stress, initiating intrinsic apoptosis, which limits the therapeutic potential of adoptively transferred T cells.
COMMUNICATIONS BIOLOGY
(2023)
Article
Chemistry, Analytical
Ping Li, Jie Wang, Mengqiu Gao, Jue Wang, Yi Ma, Yueqing Gu
Summary: Extracellular vesicles (EVs) have emerged as a promising tumor biomarker, offering benefits for cancer diagnosis, but the practicality of EV assays remains challenging due to various factors. A membrane-based biosensor has been developed for precise and sensitive EV identification, integrating EV capture and detection based on EV membrane features, showing great potential for clinical prostate cancer diagnosis.
ANALYTICAL CHEMISTRY
(2021)
Article
Multidisciplinary Sciences
Xiaoman Zhang, Toshihide Takeuchi, Akiko Takeda, Hideki Mochizuki, Yoshitaka Nagai
Summary: This study comprehensively characterized EV fractions derived from plasma and serum, and found that serum EVs contain more particles and enriched platelet-associated proteins compared to plasma EVs. Therefore, the choice of plasma or serum as a source of blood EVs may qualitatively and quantitatively alter the characteristics of EVs.
Article
Multidisciplinary Sciences
Hyun Ji Kim, Matthew J. Rames, Samuel Tassi Yunga, Randall Armstrong, Mayu Morita, Anh T. P. Ngo, Owen J. T. McCarty, Fehmi Civitci, Terry K. Morgan, Thuy T. M. Ngo
Summary: This study examined the impact of differential centrifugation and freeze/thaw on the profiles of extracellular vesicles (EVs) and cell-free messenger RNA (cf-mRNA) in plasma using flow cytometric analysis. The results revealed that the differential centrifugation and post-freeze/thaw processing affected the EV subpopulations and cf-mRNA levels. Freezing plasma containing residual platelets resulted in irreversible ex vivo generation of EV subpopulations and cf-mRNA transcripts.
SCIENTIFIC REPORTS
(2022)
Article
Multidisciplinary Sciences
Zhaohan Wei, Xiaoqiong Zhang, Tuying Yong, Nana Bie, Guiting Zhan, Xin Li, Qingle Liang, Jianye Li, Jingjing Yu, Gang Huang, Yuchen Yan, Zelong Zhang, Bixiang Zhang, Lu Gan, Bo Huang, Xiangliang Yang
Summary: This study demonstrates that metformin-loaded mannose-modified macrophage-derived microparticles can reprogram the tumor immune microenvironment, enhance responses to anti-PD-1 therapy, and promote infiltration of CD8(+) T cells into tumor tissues.
NATURE COMMUNICATIONS
(2021)
Article
Cell Biology
Nasibeh Karimi, Razieh Dalirfardouei, Tomas Dias, Jan Lotvall, Cecilia Lasser
Summary: This study reveals the presence of multiple subpopulations of extracellular vesicles (EVs) carrying different tetraspanins in both plasma and serum. The sampling methods and centrifugation protocols used for blood can impact EV analysis results.
JOURNAL OF EXTRACELLULAR VESICLES
(2022)
Article
Nanoscience & Nanotechnology
Sijun Pan, Yan Zhang, Auginia Natalia, Carine Z. J. Lim, Nicholas R. Y. Ho, Balram Chowbay, Tze Ping Loh, John K. C. Tam, Huilin Shao
Summary: The platform developed in this study leverages circulating extracellular vesicles for activity-based assessment of tumour-specific drug-target interactions and can be used for clinical cancer treatment monitoring.
NATURE NANOTECHNOLOGY
(2021)
Article
Multidisciplinary Sciences
Andrew A. Lin, Hanfei Shen, Griffin Spychalski, Erica L. Carpenter, David Issadore
Summary: In this study, finite-element simulation and experimental characterization were used to elucidate design rules for isolating EV subpopulations from blood using TENPO chips. The utility of this approach was demonstrated by reducing device background without sacrificing target EV recovery. Comparison with gold-standard methods showed its wide application and modularity in targeting EV subpopulations from multiple disease models.
SCIENTIFIC REPORTS
(2023)
Article
Multidisciplinary Sciences
Shizhen Shen, Zhangjin Shen, Conghui Wang, Xiaodong Wu, Lingfang Wang, Lele Ye, Songfa Zhang, Xiaodong Cheng
Summary: In this study, a method for extracting extracellular vesicles (EVs) from epithelial ovarian cancer (EOC) tissues was optimized to investigate the effect of cryopreservation on the phenotypes, contents, and biological functions of the extracted EVs. The results showed that cryopreservation affected the yield and distribution of EVs, but not their morphology or particle mass ratio. The storage of tissues at -80°C increased both small and large EV yields, while the storage of lysates at -80°C decreased large EV yield and increased small EV yield. The cryopreserved EOC tissue retained a high percentage of proteins and miRNAs, and the extracted EVs could promote angiogenesis and invasive migration ability in human endothelial cells. This suggests that cryopreserved EOC tissue has clinical value for studying tissue-derived EVs, especially those related to EV proteins.
Article
Obstetrics & Gynecology
Forough Mahdavinezhad, Mohammad Ali Sadighi Gilani, Roghaye Gharaei, Zhaleh Ashrafnezhad, Jamal Valipour, Maryam Shabani Nashtaei, Fardin Amidi
Summary: Research finds that seminal plasma microvesicles and exosomes can protect spermatozoa during cryopreservation, improving sperm motility, morphology, and viability, while reducing oxidative stress and DNA damage.
REPRODUCTIVE BIOMEDICINE ONLINE
(2022)
Article
Cell Biology
Nikolaos Panagiotou, Dagmara McGuinness, Armand M. G. Jaminon, Barend Mees, Colin Selman, Leon Schurgers, Paul G. Shiels
Summary: Extracellular vesicles (EVs) secreted by cells play a role in repairing cellular and tissue damage. Microvesicles (MVs) from stem cell regulatory cells have been found to enhance the repair of human dermal fibroblasts (HDFs) and mesenchymal stem cells (MSCs) under stress, but this effect is age and stress specific. These findings provide insights into the roles of EVs in tissue repair and ageing.
Article
Multidisciplinary Sciences
V. M. Ukrainskaya, O. E. Musatova, D. V. Volkov, D. S. Osipova, D. S. Pershin, A. M. Moysenovich, E. G. Evtushenko, E. A. Kulakovskaya, E. G. Maksimov, H. Zhang, Y. P. Rubtsov, M. A. Maschan, A. V. Stepanov, A. G. Gabibov
Summary: Tumor-derived extracellular vesicles (EVs) play an active role in tumor metastasis and immunosuppression in the tumor microenvironment. These EVs can carry tumor surface molecules, such as tumor-associated antigens (TAAs) and/or checkpoint inhibitors, and potentially interact with T cells or CAR T cells. The interaction between EVs and CAR T cells can change the outcome of CAR T-based cancer immunotherapy and affect the function of CAR T cells. EVs can also serve as adjuvants and antigenic components in antitumor vaccines. Chronic exposure of CD19-CAR T cells to CD19(+)EVs leads to activation and systemic exhaustion in an antigen-specific manner, accompanied by impaired cytotoxic activity in vitro.
SCIENTIFIC REPORTS
(2023)
Article
Multidisciplinary Sciences
Asmaa M. Zahran, Omnia El-Badawy, Wageeh A. Ali, Zainab Gaber Mahran, Essam Eldeen M. O. Mahran, Amal Rayan
Summary: This study found significant differences in hematologic parameters between COVID-19 malignant and non-malignant patients, with a significant accumulation of microparticles and activated platelets in the blood of affected patients. These results suggest that circulating microparticles and activated platelets may be promising prognostic biomarkers for the outcome of COVID-19 patients.
Article
Gastroenterology & Hepatology
Komal Ramani, Aaron E. Robinson, Joshua Berlind, Wei Fan, Aushinie Abeynayake, Aleksandra Binek, Lucia Barbier-Torres, Mazen Noureddin, Nicholas N. Nissen, Zehra Yildirim, Ebru Erbay, Jose M. Mato, Jennifer E. Van Eyk, Shelly C. Lu
Summary: Our study identified a SAMe-sensitive mechanism of LARP1 phosphorylation that may be involved in the progression from NASH to HCC.
Article
Chemistry, Analytical
Philipp Nitschke, Samantha Lodge, Torben Kimhofer, Reika Masuda, Sze-How Bong, Drew Hall, Hartmut Schaefer, Manfred Spraul, Nils Pompe, Tammo Diercks, Ganeko Bernardo-Seisdedos, Jose M. Mato, Oscar Millet, Daniella Susic, Amanda Henry, Emad M. El-Omar, Elaine Holmes, John C. Lindon, Jeremy K. Nicholson, Julien Wist
Summary: This study presents a J-Edited Diffusional (JEDI) proton NMR spectroscopic approach that selectively enhances signals from inflammatory markers in blood serum. The results show that JEDI is an improved method for selectively investigating inflammatory signals in serum and may have widespread diagnostic applicability to disease states associated with systemic inflammation.
ANALYTICAL CHEMISTRY
(2022)
Article
Biophysics
Maider Bizkarguenaga, Chiara Bruzzone, Ruben Gil-Redondo, Itxaso SanJuan, Itziar Martin-Ruiz, Diego Barriales, Ainhoa Palacios, Samuel T. Pasco, Beatriz Gonzalez-Valle, Ana Lain, Lara Herrera, Aida Azkarate, Miguel Angel Vesga, Cristina Eguizabal, Juan Anguita, Nieves Embade, Jose M. Mato, Oscar Millet
Summary: COVID-19 is a systemic infectious disease that affects multiple organs, with half of the patients showing abnormal metabolism six months after infection. Most patients are asymptomatic or have mild symptoms, possibly reflecting the metabolic response to immune stress.
NMR IN BIOMEDICINE
(2022)
Article
Multidisciplinary Sciences
Lucia Barbier-Torres, Ben Murray, Jin Won Yang, Jiaohong Wang, Michitaka Matsuda, Aaron Robinson, Aleksandra Binek, Wei Fan, David Fernandez-Ramos, Fernando Lopitz-Otsoa, Maria Luque-Urbano, Oscar Millet, Nirmala Mavila, Hui Peng, Komal Ramani, Roberta Gottlieb, Zhaoli Sun, Suthat Liangpunsakul, Ekihiro Seki, Jennifer E. Van Eyk, Jose M. Mato, Shelly C. Lu
Summary: MAT alpha 1 plays a role in reducing activity and causing mitochondrial dysfunction in alcohol-associated liver disease (ALD). This study found that the peptidyl-prolyl cis/trans isomerase PIN1 and kinase CK2 mediate the selective depletion of mitochondrial MAT alpha 1 in ALD. Blocking the interaction between PIN1 and MAT alpha 1 increased mitochondrial levels of MAT alpha 1 and protected against alcohol-induced mitochondrial dysfunction and fat accumulation.
NATURE COMMUNICATIONS
(2022)
Article
Biochemistry & Molecular Biology
Ruben Rodriguez-Agudo, Naroa Goikoetxea-Usandizaga, Marina Serrano-Macia, Pablo Fernandez-Tussy, David Fernandez-Ramos, Sofia Lachiondo-Ortega, Irene Gonzalez-Recio, Claudia Gil-Pitarch, Maria Mercado-Gomez, Laura Moran, Maider Bizkarguenaga, Fernando Lopitz-Otsoa, Petar Petrov, Miren Bravo, Sebastiaan Martijn Van Liempd, Juan Manuel Falcon-Perez, Amaia Zabala-Letona, Arkaitz Carracedo, Jose Vicente Castell, Ramiro Jover, Luis Alfonso Martinez-Cruz, Teresa Cardoso Delgado, Francisco Javier Cubero, Maria Isabel Lucena, Raul Jesus Andrade, Jon Mabe, Jorge Simon, Maria Luz Martinez-Chantar
Summary: The study demonstrates that anti-miR-873-5p is effective in combating APAP-induced liver injury by restoring GNMT and the methionine cycle to prevent mitochondrial dysfunction and activate hepatocyte proliferative response.
Article
Biochemistry & Molecular Biology
Oiana Telleria, Oihane E. Alboniga, Marc Clos-Garcia, Beatriz Nafria-Jimenez, Joaquin Cubiella, Luis Bujanda, Juan Manuel Falcon-Perez
Summary: In this study, metabolomics analysis of fecal samples was conducted to identify significant metabolites in CRC and AA patients. A predictive model was established with a high accuracy of 91.67%.
Article
Biochemistry & Molecular Biology
Guillermo Bordanaba-Florit, Sebastiaan van Liempd, Diana Cabrera, Felix Royo, Juan Manuel Falcon-Perez
Summary: This study developed a method for the extraction and quantification of different classes of steroid hormones using high-resolution liquid chromatography coupled time-of-flight mass spectrometry. The method was tested and applied in rat and human samples, measuring 11 analytes in various tissues and biofluids.
Article
Biochemistry & Molecular Biology
Marta Clos-Sansalvador, Sergio G. Garcia, Miriam Moron-Font, Charles Williams, Niels-Christian Reichardt, Juan M. Falcon-Perez, Antoni Bayes-Genis, Santiago Roura, Marcella Franquesa, Marta Monguio-Tortajada, Francesc E. Borras
Summary: This study reveals the importance of N-glycosylation in MSC-EV for their interaction with endothelial cells and associated functions, such as cell uptake, migration, and angiogenesis.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Cell Biology
Patricia de la Cruz-Ojeda, Tobias Schmid, Loreto Boix, Manuela Moreno, Victor Sapena, Juan M. Praena-Fernandez, Francisco J. Castell, Juan Manuel Falcon-Perez, Maria Reig, Bernhard Bruene, Miguel A. Gomez-Bravo, Alvaro Giraldez, Jordi Bruix, Maria T. Ferrer, Jordi Muntane
Summary: The study reveals that Sorafenib regulates a specific miRNA signature, where miR-200c-3p, miR-222-5p, and miR-512-3p have prognostic value and contribute to treatment response.
Article
Cell Biology
Muireann Ni Bhaoighill, Juan M. Falcon-Perez, Felix Royo, Andrew R. Tee, Jason P. Webber, Elaine A. Dunlop
Summary: This study investigates the role of extracellular vesicles (EVs) in modulating the tumor microenvironment and their impact on the development of TSC tumors. It shows that EVs secreted from TSC2-deficient cells contain a specific protein cargo that promotes cell viability, proliferation, and growth factor secretion in the tumor microenvironment. The study also demonstrates that rapamycin can alter the cargo of EVs and reduce their ability to promote cell proliferation.
JOURNAL OF EXTRACELLULAR VESICLES
(2023)
Article
Microbiology
Felix Royo, Hector Tames, Guillermo Bordanaba-Florit, Diana Cabrera, Maria Azparren-Angulo, Clara Garcia-Vallicrosa, Abelardo Margolles, Lorena Ruiz, Patricia Ruas-Madiedo, Juan M. Falcon-Perez
Summary: This study used mice as a model and fed them with a bacterial strain of B. adolescentis that can degrade glutamate and convert it into GABA. The bacterium can survive in the gastric tract and the animals reduce their blood glutamate concentration over time. The results suggest that an oral diet with this probiotic-type bacteria could reduce the concentration of glutamate in the blood, providing a reference for clinical trial studies in patients with chronic diseases.
MICROBIOLOGY SPECTRUM
(2023)
Article
Biochemistry & Molecular Biology
Oihane E. Alboniga, Joaquin Cubiella, Luis Bujanda, Maria Encarnacion Blanco, Borja Lanza, Cristina Alonso, Beatriz Nafria, Juan Manuel Falcon-Perez
Summary: Colorectal cancer (CRC) is increasing in incidence and is currently the third most common cancer. Non-invasive, specific, and accurate diagnostic tools are needed for early diagnosis and prognosis. This study evaluated the feasibility of using extracts from fecal occult blood (FOB) kits for metabolomics studies, and identified cholesteryl esters with polyunsaturated fatty acids (PUFAs) as relevant metabolites for separating CRC patients from control individuals and adenoma patients. The results highlight the advantage of using the same FOB samples for multiple analyses and suggest potential future perspectives in lipid metabolism.
Review
Biochemistry & Molecular Biology
Eduardo Anitua, Maria Troya, Juan Manuel Falcon-Perez, Silvia Lopez-Sarrio, Esperanza Gonzalez, Mohammad H. Alkhraisat
Summary: The use of platelet-rich plasma (PRP) has attracted increasing interest due to its rich content of growth factors, cytokines, chemokines, and other biomolecules. Recent research has focused on platelets' released extracellular vesicles (EVs) and their role in intercellular communication. This review aims to compile the current evidence on PRP-derived EVs and evaluate their advantages and limitations for clinical applications.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Review
Biochemistry & Molecular Biology
Clara Garcia-Vallicrosa, Juan M. Falcon-Perez, Felix Royo
Summary: The human CERS2 gene encodes a protein called CERS2, which has been found to have antitumor effects in various types of cancer, but promotes tumor growth in adenocarcinoma. This review focuses on the influence of CERS2 in bladder cancer, including its structure, activity, and the miRNAs that regulate its expression. Mechanistically, CERS2 may act as an antitumor protein through the production of long-chain ceramides, interaction with vacuolar ATPase, and inhibition of mitochondrial fission. Additionally, the expression of CERS2 in bladder cancer and its association with disease progression are discussed.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Cell Biology
Fabrice Lucien, Dakota Gustafson, Metka Lenassi, Bo Li, Jacob J. Teske, Eric Boilard, Katharina Clemm von Hohenberg, Juan Manual Falcon-Perez, Alice Gualerzi, Antonia Reale, Jennifer C. Jones, Cecilia Laesser, Charlotte Lawson, Irina Nazarenko, Lorraine O'Driscoll, Ryan Pink, Pia R-M Siljander, Carolina Soekmadji, An Hendrix, Joshua A. Welsh, Kenneth W. Witwer, Rienk Nieuwland
Summary: Blood is commonly used for extracellular vesicle research, but its composition can vary due to donor, collection, and preparation protocols. To improve reproducibility, the ISEV Blood EV Task Force proposes standardized reporting of protocols and sample quality, and has created the MIBlood-EV tool to gather this information.
JOURNAL OF EXTRACELLULAR VESICLES
(2023)