Article
Medical Laboratory Technology
Cheuk-Chun Szeto, Jack Kit-Chung Ng, Winston Wing-Shing Fung, Gordon Chun-Kau Chan, Cathy Choi-Wan Luk, Ka-Bik Lai, Gang Wang, Kai-Ming Chow, Fernand Mac-Moune Lai
Summary: This study identified 6 miRNA targets that are significantly elevated in the urine of patients with IgAN, and urinary miR-106a level has excellent sensitivity for the diagnosis of IgAN. Further validation studies are needed to confirm its role in disease screening.
CLINICA CHIMICA ACTA
(2022)
Article
Urology & Nephrology
Audrey Uffing, Frank Hullekes, Leonardo Riella, Jonathan J. Hogan
Summary: Recurrent glomerular disease after kidney transplant is a significant cause of allograft failure, with incomplete knowledge on pathophysiology leading to a lack of targeted and effective therapies. In this review, specific clinical dilemmas in managing recurrent glomerular disease are highlighted, focusing on new insights into understanding and treating post-transplant diseases such as focal segmental glomerulosclerosis, membranous nephropathy, atypical hemolytic uremic syndrome, C3 glomerulopathy, amyloid light-chain (AL) amyloidosis, and IgA nephropathy.
CLINICAL JOURNAL OF THE AMERICAN SOCIETY OF NEPHROLOGY
(2021)
Article
Biochemistry & Molecular Biology
Cheuk-Chun Szeto, Ho So, Peter Yam-Kau Poon, Cathy Choi-Wan Luk, Jack Kit-Chung Ng, Winston Wing-Shing Fung, Gordon Chun-Kau Chan, Kai-Ming Chow, Fernand Mac-Moune Lai, Lai-Shan Tam
Summary: Through the study of three cohorts of lupus nephritis patients and controls, we identified specific lncRNA targets in urine that are associated with the severity and histological classification of lupus nephritis. These urinary lncRNA levels may serve as biomarkers for the disease.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Medicine, Research & Experimental
Yuki Otani, Osamu Ichii, Md. Abdul Masum, Takashi Namba, Teppei Nakamura, Yasuhiro Kon
Summary: Sex hormones play a role in maintaining immunity and renal homeostasis in mammals, and are associated with sex-dependent diseases. Castration in the murine autoimmune glomerulonephritis model BXSB/MpJ-Yaa helped reduce glomerular sclerotic lesions, indicating a potential impact of androgens on renal pathophysiology.
EXPERIMENTAL BIOLOGY AND MEDICINE
(2021)
Article
Urology & Nephrology
Richard Furie, Brad H. Rovin, Frederic Houssiau, Gabriel Contreras, Y. K. Onno Teng, Paula Curtis, Yulia Green, Mohamed Okily, Anuradha Madan, David A. Roth
Summary: The open-label extension of the BLISS-LN study showed that belimumab had good safety and efficacy in improving kidney outcomes in patients with active lupus nephritis.
CLINICAL JOURNAL OF THE AMERICAN SOCIETY OF NEPHROLOGY
(2022)
Article
Biochemical Research Methods
Chao-Jung Chen, Che-Yi Chou, Kuo-Hsiung Shu, Hung-Chun Chen, Ming-Cheng Wang, Chia-Chu Chang, Bang-Gee Hsu, Mai-Szu Wu, Yuan-Lung Yang, Wen-Ling Liao, Chieh Yang, Yu-Tien Hsiao, Chiu-Ching Huang
Summary: This study identified BRDT, CYBP, GARS, and HDGF as novel urinary UC biomarkers with high discrimination ability. These markers showed better diagnostic values compared to other reported UC markers, and a panel including BRDT, HDGF, GARS, and CYFR21.1 had high AUC values for discriminating between UC and normal or control groups.
JOURNAL OF PROTEOME RESEARCH
(2021)
Review
Biochemistry & Molecular Biology
Nicolae Pana, Cristina Capusa
Summary: This review summarizes the existing evidence supporting the role of periostin as a novel biomarker in glomerulopathies. Periostin plays an important role in progressive kidney diseases characterized by inflammation and fibrosis, and its expression is correlated with the degree of tissue damage and kidney function decline in CKD patients.
Article
Urology & Nephrology
Rania El Fekih, James Hurley, Vasisht Tadigotla, Areej Alghamdi, Anand Srivastava, Christine Coticchia, John Choi, Hazim Allos, Karim Yatim, Juliano Alhaddad, Siawosh Eskandari, Philip Chu, Albana B. Mihali, Isadora T. Lape, Mauricio P. Lima Filho, Bruno T. Aoyama, Anil Chandraker, Kassem Safa, James F. Markmann, Leonardo V. Riella, Richard N. Formica, Johan Skog, Jamil R. Azzi
Summary: The study demonstrates that urinary exosomal mRNA signatures are a powerful and noninvasive tool for screening kidney allograft rejection, with high predictive performance. The signatures can differentiate between different types of rejection and assist clinicians in therapeutic decision making.
JOURNAL OF THE AMERICAN SOCIETY OF NEPHROLOGY
(2021)
Article
Urology & Nephrology
Xiaohan Yuan, Qing Su, Hui Wang, Sufang Shi, Lijun Liu, Jicheng Lv, Suxia Wang, Li Zhu, Hong Zhang
Summary: This study compared patients with sporadic IgA nephropathy (IgAN) with thinned glomerular basement membrane (tGBM) lesions to patients without tGBM lesions and patients with thin basement membrane nephropathy (TBMN) by screening COL4A3/COL4A4/COL4A5 gene variants. The results showed that patients with IgAN-tGBM who had diagnostic variants of these genes had milder disease manifestation.
JOURNAL OF THE AMERICAN SOCIETY OF NEPHROLOGY
(2023)
Article
Urology & Nephrology
Tiffany N. Caza, Aaron J. Storey, Samar I. Hassen, Christian Herzog, Rick D. Edmondson, John M. Arthur, Daniel J. Kenan, Christopher P. Larsen
Summary: Multiple new antigenic targets have been identified in PLA2R-/THSD7A-/EXT-/NELL1-quadruple negative MN biopsies through mass spectrometry of immune complexes. These discoveries expand our understanding of the disease and may contribute to the development of new diagnostic approaches.
KIDNEY INTERNATIONAL
(2023)
Article
Urology & Nephrology
Miguel Verbitsky, Sarathbabu Krishnamurthy, Priya Krithivasan, Daniel Hughes, Atlas Khan, Maddalena Marasa, Natalie Vena, Pavan Khosla, Junying Zhang, Tze Y. Lim, Joseph T. Glessner, Chunhua Weng, Ning Shang, Yufeng Shen, George Hripcsak, Hakon Hakonarson, Iuliana Ionita-Laza, Brynn Levy, Eimear E. Kenny, Ruth J. F. Loos, Krzysztof Kiryluk, Simone Sanna-Cherchi, David R. Crosslin, Susan Furth, Bradley A. Warady, Robert P. Igo Jr, Sudha K. Iyengar, Craig S. Wong, Afshin Parsa, Harold I. Feldman, Ali G. Gharavi
Summary: The prevalence of genomic disorders (GDs) was higher in patients with chronic kidney disease (CKD) compared to controls. Children with CKD had a higher rate of GDs (3.6%) compared to adults with CKD (1.1%). GDs were associated with comorbidities such as diabetes and neuropsychiatric symptoms.
JOURNAL OF THE AMERICAN SOCIETY OF NEPHROLOGY
(2023)
Article
Urology & Nephrology
Elenjickal Elias John, Athul Thomas, Jeethu Joseph Eapen, Sabina Yusuf, Sanjeet Roy, Anna T. Valson, Vinoi George David, Santosh Varughese, Suceena Alexander
Summary: This study followed up 501 adult patients diagnosed with bacterial infection-related glomerulonephritis over a period of 12 years, showing that parainfectious GN caused predominantly by nonstreptococcal and drug-resistant bacterial infections was associated with poor kidney prognosis.
CLINICAL JOURNAL OF THE AMERICAN SOCIETY OF NEPHROLOGY
(2021)
Article
Urology & Nephrology
Raja Ramachandran, Shabna Sulaiman, Prabhat Chauhan, Ifeoma Ulasi, Ugochi Onu, Russell Villaneuva, Muhammad Rafiqul Alam, Fazal Akhtar, Lloyd Vincent, Gurmehar S. Aulakh, Aida Lydia Sutranto, Elena Zakharova, Vivekanand Jha
Summary: A survey conducted among kidney specialists in Asia, Africa, and Eastern Europe revealed that while most specialists can perform kidney biopsy, obtaining comprehensive biopsy results is more challenging in Africa. Additionally, a high percentage of patients in these countries have to cover the cost of diagnosis and treatment out-of-pocket.
KIDNEY INTERNATIONAL REPORTS
(2022)
Article
Urology & Nephrology
Javier Villacorta, Laura Lucientes, Elena Goicoechea, Mercedes Acevedo, Teresa Cavero, Luis Sanchez-Camara, Francisco Diaz-Crespo, Sara Gimenez-Moyano, Laura Garcia-Bermejo, Gema Fernandez-Juarez
Summary: Urinary soluble CD163 (usCD163) is an accurate biomarker for detecting active renal vasculitis and relapse, closely associated with disease activity, providing additional information for monitoring treatment response.
CLINICAL KIDNEY JOURNAL
(2021)
Article
Medicine, General & Internal
Adrienne W. S. Cohen, Eswari Vilayur
Summary: This study aimed to evaluate the prevalence, clinicopathological correlations, and outcomes of Fibrillary Glomerulonephritis (FGN) and Immunotactoid Glomerulonephritis (IT) in an Australian regional center. The results showed that FGN had a poor response to immunomodulatory therapy and a poor prognosis, while IT was associated with hematological malignancy, had a better prognosis, and responded to treatment.
INTERNAL MEDICINE JOURNAL
(2023)
Article
Biochemistry & Molecular Biology
Thiago Seraphim, Nardin Nano, Yiu Wing Sunny Cheung, Siripat Aluksanasuwan, Carolina Colleti, Yu-Qian Mao, Vaibhav Bhandari, Gavin Young, Larissa Holl, Sadhna Phanse, Yuliya Gordiyenko, Daniel R. Southworth, Carol Robinson, Visith Thongboonkerd, Lisandra M. Gava, Julio C. Borges, Mohan Babu, Leandro R. S. Barbosa, Carlos H. Ramos, Philipp Kukura, Walid A. Houry
Summary: The R2TP complex, formed by RUVBL1 and RUVBL2 ATPases, associates with PIH1D1 and RPAP3 proteins and plays a role in promoting the formation of macromolecular complexes. RPAP3 is identified as the central subunit of R2TP, linking PIH1D1 and RUVBL1/2. The study provides insights into the structure and function of the R2TP complex.
Article
Oncology
Paleerath Peerapen, Kanyarat Sueksakit, Wanida Boonmark, Sunisa Yoodee, Visith Thongboonkerd
Summary: Loss of ARID1A is associated with higher grade of colorectal cancer. In this study, knockdown of ARID1A resulted in decreased cell death and senescence, enhanced cell proliferation, chemoresistance and invasion. Additionally, ARID1A knockdown led to the formation of polyploid giant cancer cells, increased cell aggregation and secretion of vascular endothelial growth factor (VEGF).
Article
Medicine, Research & Experimental
Paleerath Peerapen, Wanida Boonmark, Visith Thongboonkerd
Summary: This study investigated the protective effects and mechanisms of trigonelline against kidney stone formation. The results showed that trigonelline can reduce stone formation by inhibiting crystallization, crystal growth, and crystal-cell adhesion. Additionally, trigonelline downregulated crystal receptors on the apical membranes of renal tubular cells.
BIOMEDICINE & PHARMACOTHERAPY
(2022)
Article
Biochemistry & Molecular Biology
Chadanat Noonin, Paleerath Peerapen, Sunisa Yoodee, Chompunoot Kapincharanon, Rattiyaporn Kanlaya, Visith Thongboonkerd
Summary: This study clarifies the inhibitory roles of native human urinary THP in calcium oxalate monohydrate (COM) kidney stone formation. THP reduces COM crystal size, inhibits crystal growth and aggregation, and prevents crystal-cell adhesion. These inhibitory effects are predominantly mediated via the high affinity of THP with calcium ions.
CHEMICO-BIOLOGICAL INTERACTIONS
(2022)
Article
Biochemistry & Molecular Biology
Rattiyaporn Kanlaya, Chompunoot Kapincharanon, Kedsarin Fong-ngern, Visith Thongboonkerd
Summary: This study investigated the potential of epigallocatechin-3-gallate (EGCG) to reverse epithelial-mesenchymal transition (EMT) and restore normal cell morphology. The results demonstrated that EGCG effectively reversed EMT and inhibited tumor and fibrosis.
JOURNAL OF NUTRITIONAL BIOCHEMISTRY
(2022)
Article
Toxicology
Chanettee Chanthick, Visith Thongboonkerd
Summary: This study found that hyaluronic acid promotes the formation of calcium oxalate kidney stones by enhancing crystal growth, adhesion to cells, and invasion through the extracellular matrix. These findings provide an explanation for the higher levels of hyaluronic acid in the urine of stone patients.
TOXICOLOGY IN VITRO
(2022)
Article
Biochemistry & Molecular Biology
Rattiyaporn Kanlaya, Visith Thongboonkerd
Summary: This study demonstrates that persistent infection by E. coli may contribute to COM metabolic stone formation by translocation of ezrin to apical membranes, thereby promoting COM crystal-cell adhesion.
CELLULAR AND MOLECULAR LIFE SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Sunisa Yoodee, Paleerath Peerapen, Sirikanya Plumworasawat, Visith Thongboonkerd
Summary: This study found that heat-shock protein 90 (HSP90) and its four functional domains play important roles in the formation of kidney stones, promoting the crystallization, growth, aggregation, adhesion onto renal cells, and invasion through the extracellular matrix (ECM) of calcium oxalate monohydrate (COM) crystals.
CELLULAR AND MOLECULAR LIFE SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Visith Thongboonkerd, Rattiyaporn Kanlaya
Summary: Exosomes are nanoscale vesicles derived from late endosomes and released from cells. They selectively encapsulate biomolecules and play roles in cell-cell communication. In kidney diseases, exosomes have divergent roles in pathogenesis, diagnostics, prognostics, and therapeutics.
INTERNATIONAL JOURNAL OF BIOCHEMISTRY & CELL BIOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Sakdithep Chaiyarit, Visith Thongboonkerd
Summary: The research reveals that oxidative modification can reverse the modulatory activity of the urinary protein uromodulin (UMOD), promoting calcium oxalate stone formation. Oxidized forms of UMOD facilitate CaOx crystallization and crystal growth, while having no effect on crystal aggregation.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2022)
Review
Medicine, Research & Experimental
Sudarat Hadpech, Visith Thongboonkerd
Summary: CyPA is an immunophilin widely expressed in various cell types and tissues, with higher expression in proximal tubular epithelial cells in the kidney. Changes in CyPA expression and secretion play important roles in kidney diseases. Urinary CyPA shows promise as a diagnostic/prognostic marker in several kidney diseases. Understanding the theranostic roles of CyPA in kidney diseases may offer insights for designing new therapeutic interventions and prevention strategies.
Article
Medicine, General & Internal
Kanyarat Sueksakit, Visith Thongboonkerd
Summary: The study found that adding 2.5% fetal bovine serum to artificial urine can prolong the deformation time of MDCK renal tubular cells and enhance polarization, but may increase cell death rate. Therefore, the use of AU-Siriraj+2.5% FBS is recommended for studying cell biology and function.
INTERNATIONAL JOURNAL OF MEDICAL SCIENCES
(2021)
Article
Chemistry, Analytical
Chadanat Noonin, Chompunoot Kapincharanon, Kanyarat Sueksakit, Rattiyaporn Kanlaya, Visith Thongboonkerd
Summary: This study utilized tandem fast protein liquid chromatography to isolate THP from urine, comparing its efficiency and purity with two conventional methods. The results showed that the tandem FPLC system is most effective for selectively purifying monomeric and aggregated forms of THP, while diatomaceous earth adsorption is preferred for general purification.
ANALYTICAL METHODS
(2021)
Review
Medicine, Research & Experimental
Chadanat Noonin, Visith Thongboonkerd
Summary: The inflammasome is a complex of proteins in the cytoplasm of cells activated by infectious and non-infectious stimuli, while exosomes are extracellular vesicles that play important roles in intercellular communication. The review discusses the interaction between exosomes and inflammasomes in inflammatory responses, highlighting the effects of inflammasome activation on exosomal secretion. Additionally, it explores the dual effects of exosomes on inhibiting and promoting inflammasome activation, and provides insights into the therapeutic potential of exosomes in human diseases.
Article
Cell Biology
Uta Erdbrugger, Charles J. Blijdorp, Irene V. Bijnsdorp, Francesc E. Borras, Dylan Burger, Benedetta Bussolati, James Brian Byrd, Aled Clayton, James W. Dear, Juan M. Falcon-Perez, Cristina Grange, Andrew F. Hill, Harry Holthofer, Ewout J. Hoorn, Guido Jenster, Connie R. Jimenez, Kerstin Junker, John Klein, Mark A. Knepper, Erik H. Koritzinsky, James M. Luther, Metka Lenassi, Janne Leivo, Inge Mertens, Luca Musante, Eline Oeyen, Maija Puhka, Martin E. van Royen, Catherine Sanchez, Carolina Soekmadji, Visith Thongboonkerd, Volkert van Steijn, Gerald Verhaegh, Jason P. Webber, Kenneth Witwer, Peter S. T. Yuen, Lei Zheng, Alicia Llorente, Elena S. Martens-Uzunova
Summary: Urine is commonly used for clinical diagnosis and biomedical research, and the discovery of extracellular vesicles (EV) in urine has opened a new scientific field. However, methods for isolating and characterizing urinary extracellular vesicles (uEVs) need further optimization and standardization to advance scientific research and translation into clinical practice. Recommendations for improved rigor, reproducibility, and interoperability in uEV research are provided to address current challenges and gaps in the field.
JOURNAL OF EXTRACELLULAR VESICLES
(2021)