Article
Cell Biology
Zhiran Zou, Xiao Long, Qian Zhao, Yandong Zheng, Moshi Song, Shuai Ma, Yaobin Jing, Si Wang, Yifang He, Concepcion Rodriguez Esteban, Nanze Yu, Jiuzuo Huang, Piu Chan, Ting Chen, Juan Carlos Izpisua Belmonte, Weiqi Zhang, Jing Qu, Guang-Hui Liu
Summary: Single-cell RNA sequencing of human eyelid skin revealed progressive accumulation of photoaging-related changes and increased chronic inflammation with age. Transcriptional factors involved in the developmental process showed early-onset decline during aging. Inhibition of key transcription factors HES1 and KLF6 compromised cell proliferation, increased inflammation, and accelerated cellular senescence during aging.
DEVELOPMENTAL CELL
(2021)
Article
Cell Biology
Chia-Lang Hsu, Chieh-Fan Yin, Yi-Wen Chang, Ya-Chih Fan, Shih-Han Lin, Yu-Ching Wu, Hsuan-Cheng Huang, Hsueh-Fen Juan
Summary: SNHG1 is an oncogenic long non-coding RNA that is aberrantly expressed in different tumor types. It plays a crucial role in maintaining the identity of neuroblastoma (NB) through chromatin regulation and interacts with core regulatory circuitry transcription factors.
CELL DEATH & DISEASE
(2022)
Article
Gastroenterology & Hepatology
Naga Chalasani, Raj Vuppalanchi, Craig Lammert, Samer Gawrieh, Jerome V. Braun, Jiali Zhuang, Arkaitz Ibarra, David A. Ross, Michael Nerenberg, Stephen R. Quake, John J. Sninsky, Shusuke Toden
Summary: This study characterized the circulating transcriptome of primary sclerosing cholangitis (PSC) using cell-free messenger RNA (cf-mRNA) sequencing. Differential expression analysis identified 1407 dysregulated genes in PSC and found common genes involved in liver pathophysiology. A diagnostic classifier based on liver-specific genes was developed to distinguish PSC from healthy controls.
HEPATOLOGY COMMUNICATIONS
(2023)
Article
Multidisciplinary Sciences
Yi Zhang, Yuzhi Wang, Xiaoqing Yin, Yi Huang
Summary: This study identifies pyroptosis-related lncRNAs as promising prognostic factors and therapeutic targets for lung squamous cell carcinoma. A signature of 11 lncRNAs is established to predict patient prognosis and guide treatment decisions.
SCIENTIFIC REPORTS
(2022)
Article
Cell & Tissue Engineering
Denise Philipp, Michelle Holthaus, Vida Basoah, Kurt Pfannkuche, Laura Suhr, Thorsten Wahlers, Adnana Paunel-Goerguelue
Summary: Myocardial hypertrophy is a predictor of adverse cardiovascular outcomes, and mesenchymal stem cells (MSCs) have been suggested to reduce cardiac hypertrophy and the progression to heart failure. Preconditioning of MSCs can improve their paracrine activity, and the secretome of preconditioned MSCs can trigger regression of hypertrophy in induced pluripotent stem cell-derived cardiomyocytes. The expression of Nor1 in induced pluripotent stem cell-derived cardiomyocytes is associated with hypertrophic growth, while VEGF secreted by preconditioned MSCs can induce regression of hypertrophy. Delivery of MSC-derived secretome may represent a therapeutic strategy to limit cardiac hypertrophy, pending further in vivo studies.
STEM CELLS INTERNATIONAL
(2021)
Article
Cell Biology
Wei Wang, Fangfang Li, Xiaoyang Lai, Han Liu, Shuting Wu, Yunqin Han, Yunfeng Shen
Summary: The study revealed that exosomes derived from palmitic acid-treated hepatocytes enriched in miR-107 played a dual role in regulating LX-2 cell activation, both by activating Wnt signaling in LX-2 cells and promoting activation through Raf/MEK/ERK signaling pathway by targeting Foxp1 in CD4+T lymphocytes.
CELL DEATH DISCOVERY
(2021)
Article
Biochemistry & Molecular Biology
Wei Wei, Nicholas M. Riley, Andrew C. Yang, Joon T. Kim, Stephanie M. Terrell, Veronica L. Li, Marta Garcia-Contreras, Carolyn R. Bertozzi, Jonathan Z. Long
Summary: This study introduced a proximity biotinylation strategy to label, detect, and enrich secreted polypeptides in a cell type-selective manner in mice, resulting in a proteomic atlas of secretomes from various cell types. The research confirmed known cell type-protein pairs, identified new secreted polypeptides distinguishing between cell types, and uncovered a dynamic nutrient-dependent reprogramming of hepatocyte secretome. The strategy enables dynamic and cell type-specific dissection of the plasma proteome and intercellular signaling mediators.
NATURE CHEMICAL BIOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Noemi Monti, Roberto Verna, Aurora Piombarolo, Alessandro Querqui, Mariano Bizzarri, Valeria Fedeli
Summary: The traditional theory on the influence of DNA mutations on carcinogenesis is facing increasing controversial results, calling for a restructuring of theoretical models. New perspectives emphasize the interaction between cells and their microenvironment as a critical pathogenetic level, offering the opportunity to resolve seemingly inexplicable paradoxes observed in cancer biology.
Article
Multidisciplinary Sciences
Leticia de Almeida, Alexandre L. N. da Silva, Tamara S. Rodrigues, Samuel Oliveira, Adriene Y. Ishimoto, Amanda A. Seribelli, Amanda Becerra, Warrison A. Andrade, Marco A. Ataide, Camila C. S. Caetano, Keyla S. G. de Sa, Natalia Pelisson, Ronaldo B. Martins, Juliano de Paula Souza, Eurico Arruda, Sabrina S. Batah, Ricardo Castro, Fabiani G. Frantz, Fernando Q. Cunha, Thiago M. Cunha, Alexandre T. Fabro, Larissa D. Cunha, Paulo Louzada-Junior, Rene D. R. de Oliveira, Dario S. Zamboni
Summary: This study identifies niclosamide as a potential FDA-approved drug for treating COVID-19 by inhibiting inflammasome activation and SARS-CoV-2 replication. Niclosamide shows strong inhibitory effects on inflammasome activation in human monocytes and PBMCs from COVID-19 patients in vitro, as well as in an in vivo mouse model of SARS-CoV-2 infection.
Article
Neurosciences
Tyler J. Dause, Jiyeon K. Denninger, Bryon M. Smith, Elizabeth D. Kirby
Summary: This review focuses on investigating the autocrine and paracrine functions of the endogenous NSC secretome across life. Evidence shows that the NSC secretome is critical for how endogenous NSCs regulate themselves and their niche, but there are gaps in current literature, particularly in the clinically-relevant domain of endogenous NSC paracrine function in the injured CNS.
EXPERIMENTAL NEUROLOGY
(2022)
Article
Chemistry, Multidisciplinary
Jae Il Joo, Hwa-Jeong Park, Kwang-Hyun Cho
Summary: Accumulated genetic alterations in cancer cells disrupt the stimulus-response relationships, resulting in uncontrolled proliferation. However, the complex molecular interaction network within cells suggests that it may be possible to restore these relationships by controlling hidden molecular switches. A system framework is presented for analyzing cellular input-output relationships and identifying molecular switches that can normalize the distorted relationships based on Boolean network modeling and dynamics analysis. The potential for reversion is demonstrated using cancer molecular networks and a case study on bladder cancer.
Editorial Material
Multidisciplinary Sciences
Jeffrey A. Farrell
Summary: CellOracle, a computational tool, can predict the interaction of gene networks in programming cell identity during embryonic development. This tool will contribute to a better understanding of the regulation of development.
Article
Cell & Tissue Engineering
Marwan M. Merkhan, Matthew T. Shephard, Nicholas R. Forsyth
Summary: Modulation of environmental oxygen alters both the concentration and composition of the human mesenchymal stem cell (hMSC) secretome, which is likely to have significant implications for improving understanding and efficacy of hMSC-based therapeutics.
JOURNAL OF TISSUE ENGINEERING
(2021)
Editorial Material
Multidisciplinary Sciences
Alain Chedotal
Summary: The GPC3-Unc5 protein complex is found to regulate cell migration in embryos and cancer, indicating a similarity in the migration mechanisms of developing neurons and metastatic cancer cells.
Article
Multidisciplinary Sciences
Xitiz Chamling, Alyssa Kallman, Weixiang Fang, Cynthia A. Berlinicke, Joseph L. Mertz, Prajwal Devkota, Itzy E. Morales Pantoja, Matthew D. Smith, Zhicheng Ji, Calvin Chang, Aniruddha Kaushik, Liben Chen, Katharine A. Whartenby, Peter A. Calabresi, Hai-Quan Mao, Hongkai Ji, Tza-Huei Wang, Donald J. Zack
Summary: The study reveals significant transcriptional heterogeneity of human oligodendrocyte progenitor cells (hOPCs) and identifies a potential cytokine-responsive hOPC subset, as well as candidate regulatory genes/networks that define the identity of these sub-populations.
NATURE COMMUNICATIONS
(2021)