Article
Chemistry, Medicinal
Ariful Islam, Takumi Sakamoto, Qing Zhai, Md Muedur Rahman, Md Al Mamun, Yutaka Takahashi, Tomoaki Kahyo, Mitsutoshi Setou
Summary: This study applied three different tools of mass spectrometry imaging (MSI) to visualize drugs and their metabolites in mice. Among the three MSI tools, iMScope QT demonstrated better detection capability and higher speed for rapid mapping of targeted drugs. The study found that imipramine and its metabolites predominantly accumulated in the renal cortex of mice, while chloroquine and its metabolites accumulated highly in the renal pelvis and renal medulla. In addition, different accumulation patterns were observed in various regions of the mouse brain. These findings contribute to the understanding of the clinical properties, efficacy, and potential side effects of these drugs, and demonstrate the potential of iMScope QT for rapid mapping of small drugs and their metabolites in biological samples.
Article
Environmental Sciences
Yanyan Chen, Lilong Jiang, Rong Zhang, Zhangsheng Shi, Chengyi Xie, Yanjun Hong, Jianing Wang, Zongwei Cai
Summary: This study used AP-MALDI MSI to reveal the spatial distributions of PFOS in mouse kidneys and its adverse effects on lipid metabolism. PFOS was found to accumulate mainly in the renal pelvis and outer cortex regions, causing damage to the kidneys. Significant down-regulation of certain lipids and up-regulation of others were observed in different regions of the kidneys between control and PFOS-exposed mice.
SCIENCE OF THE TOTAL ENVIRONMENT
(2022)
Article
Engineering, Environmental
Xin Diao, Chengyi Xie, Guangshan Xie, Yuanyuan Song, Yanshan Liang, Ruijin Li, Chuan Dong, Lin Zhu, Jianing Wang, Zongwei Cai
Summary: This study utilized advanced techniques to investigate the spatial distribution of lipids in rat brains after exposure to high levels of PM2.5. The findings suggest a possible association between PM2.5 exposure and neurological damage, potentially mediated by reductions in sulfatides and subsequent neuroinflammation in the corpus callosum region.
ENVIRONMENTAL SCIENCE & TECHNOLOGY LETTERS
(2022)
Article
Biochemistry & Molecular Biology
Kim Frisch, Kristine Lykke Nielsen, Simona Francese
Summary: Although DNA profiling has been developed, fingerprints still play an important role in identifying suspects. However, it is more challenging to identify overlapping fingermarks. In this study, we investigate whether Matrix-Assisted Laser Desorption Ionisation Mass Spectrometry Imaging can separate fingermarks from the same donor deposited at different times based on variability in their composition. We also explore the possibility of determining the deposition time using this method.
Review
Biochemical Research Methods
Qiuqin Zhou, Annabelle Fueloep, Carsten Hopf
Summary: MALDI-MSI is a rapidly growing technique for visualizing the distribution of biomolecules in tissue sections, with challenges such as sensitivity and specificity. Recent developments in small organic MALDI matrices and on-tissue chemical derivatization reagents play a crucial role in improving image quality and expanding application areas.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2021)
Article
Biochemistry & Molecular Biology
Manoj Yadav, Prem Prashant Chaudhary, Brandon N. D'Souza, Jacquelyn Spathies, Ian A. Myles
Summary: The study found that different fixation methods significantly impact the analysis of metabolite content and distribution. Samples fixed in formalin and paraffin and fresh frozen samples showed substantial differences in the sharing of metabolites, but both methods allowed for downstream fluorescent staining.
Article
Oncology
Fanny Boyaval, Hans Dalebout, Rene Van Zeijl, Wenjun Wang, Arantza Farina-Sarasqueta, Guinevere S. M. Lageveen-Kammeijer, Jurjen J. Boonstra, Liam A. McDonnell, Manfred Wuhrer, Hans Morreau, Bram Heijs
Summary: This study investigates the changes in sugar molecules during the progression of colorectal cancer (CRC) and finds that these sugar signatures can clearly differentiate different stages of CRC progression, especially between pre-cancerous and cancerous tissue. The study also observes the spreading of glycosylation signatures into the tumor microenvironment.
Article
Nutrition & Dietetics
Huu-Nghi Nguyen
Summary: Matrix-assisted laser desorption/ionization mass spectrometry imaging (MALDI-MSI) is a powerful tool for visualizing the absorption, distribution, and metabolism processes of food polyphenols, providing valuable insights into their pharmacological properties.
JOURNAL OF NUTRITIONAL SCIENCE AND VITAMINOLOGY
(2022)
Article
Medicine, Research & Experimental
Xiwei Fan, Reuben S. E. Young, Antonia Rujia Sun, Brett R. Hamilton, Udhaya Nedunchezhiyan, Ross Crawford, Stephen J. Blanksby, Indira Prasadam
Summary: This study used mass spectrometry imaging to visualize the distribution of lipase enzymes in bone and cartilage tissue sections and correlated them with immunohistochemistry staining results. The results showed that OA patients exhibited increased expression of PLA(2)G(2)A at the superficial and deep cartilage zones, indicating that tissue-specific inhibition of PLA(2)G(2)A may be a potential therapeutic target for OA.
Article
Medicine, Research & Experimental
Verena Panitz, Saga Koncarevic, Ahmed Sadik, Dennis Friedel, Tobias Bausbacher, Saskia Trump, Vadim Farztdinov, Sandra Schulz, Philipp Sievers, Stefan Schmidt, Ina Juergenson, Stephan Jung, Karsten Kuhn, Irada Pflueger, Suraj Sharma, Antje Wick, Pauline Pfaender, Stefan Selzer, Philipp Vollmuth, Felix Sahm, Andreas von Deimling, Ines Heiland, Carsten Hopf, Peter Schulz-Knappe, Ian Pike, Michael Platten, Wolfgang Wick, Christiane A. Opitz
Summary: This study utilized LC-MS/MS combined with chemical isobaric labeling to analyze serum samples from 43 recurrent glioblastoma patients and 43 healthy controls, revealing decreased levels of Trp and its metabolites in glioblastoma patients compared to controls. Higher tumor volume was associated with lower systemic metabolite levels, and lower systemic kynurenine levels correlated with worse overall survival. Additionally, analysis of scRNA-seq data showed that genes involved in Trp metabolism were expressed in various cell types in glioblastoma, with AHR activation observed in macrophages and T cells, and high AHR activity was linked to reduced overall survival in the glioblastoma TCGA dataset.
Article
Biochemical Research Methods
Ana Claudia Hertel Pereira, Ana Carolina Auer, Lauro Biedel, Camila Medeiros de Almeida, Wanderson Romao, Denise Coutinho Endringer
Summary: The study investigates the potential use of Gliricidia sepium as a raw material for natural cosmetics. It examines the chemical composition, antioxidant potential, antimicrobial action, and toxicity of the plant. The study also explores the use of MALDI MS and MALDI MSI techniques to analyze the spatial distribution of phytochemicals on the leaf surface. The results show that G. sepium leaves contain polyphenols and tannins, with a higher flavonoid content in the methanolic extract. Several compounds with potential cosmetic applications, such as kaempferol, isorhamnetin, and fatty acids, are identified on the leaf surface. The extracts exhibit antioxidant and antimicrobial properties without cytotoxicity.
JOURNAL OF THE AMERICAN SOCIETY FOR MASS SPECTROMETRY
(2022)
Article
Biochemistry & Molecular Biology
Lisanna Paladin, Martina Bevilacqua, Sara Errigo, Damiano Piovesan, Ivan Micetic, Marco Necci, Alexander Miguel Monzon, Maria Laura Fabre, Jose Luis Lopez, Juliet F. Nilsson, Javier Rios, Pablo Lorenzano Menna, Maia Cabrera, Martin Gonzalez Buitron, Mariane Goncalves Kulik, Sebastian Fernandez-Alberti, Maria Silvina Fornasari, Gustavo Parisi, Antonio Lagares, Layla Hirsh, Miguel A. Andrade-Navarro, Andrey Kajava, Silvio C. E. Tosatto
Summary: RepeatsDB database provides annotations and classification for protein tandem repeat structures from PDB. The new version 3.0 addresses challenges of data growth and annotation needs by introducing a hierarchical classification scheme.
NUCLEIC ACIDS RESEARCH
(2021)
Article
Agriculture, Multidisciplinary
Yan-Xia Zhang, Yi-Da Zhang, Yan-Ping Shi
Summary: Exploring the spatial distribution alterations of metabolites during lentil germination is crucial for understanding its nutritional value, physiological function, and metabolic pathway. This study established an effective MALDI-MSI method to visualize the changes in spatial localization of 53 metabolites in lentils during germination. The results showed specific distribution patterns of different metabolites, with certain lipids mainly located in the cotyledons and others spreading throughout the lentil tissue during early germination. Heat map analysis demonstrated the correlation between lipid content changes and germination time, supporting the potential use of germinated lentils as nutraceutical or functional food.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2023)
Review
Biochemistry & Molecular Biology
Elvira Sgobba, Yohann Daguerre, Marco Giampa
Summary: Classic metabolomic methods have limitations in spatial localization within fine structures, while MALDI MSI provides high spatial resolution for analyzing the local complexity of biological samples. This technology has been widely used in medical sciences and is now expanding to various research areas, demonstrating its strength in characterizing spatial distributions of metabolites, lipids, and peptides in relation to biological questions.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Biochemistry & Molecular Biology
Isabella Piga, Vincenzo L'Imperio, Lucrezia Principi, Claudio Bellevicine, Nicola Fusco, Fausto Maffini, Konstantinos Venetis, Mariia Ivanova, Davide Seminati, Gabriele Casati, Lisa Pagani, Stefania Galimberti, Giulia Capitoli, Mattia Garancini, Andrea-Valer Gatti, Fulvio Magni, Fabio Pagni
Summary: This study used Matrix-Assisted Laser Desorption/Ionisation (MALDI)-Mass Spectrometry Imaging (MSI) to analyze noninvasive follicular thyroid neoplasms with papillary-like nuclear features (NIFTP). It discovered that proteomic signatures can overcome histological challenges and distinguish different types of cases.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Multidisciplinary Sciences
Sara Svensson Akusjarvi, Anoop T. Ambikan, Shuba Krishnan, Soham Gupta, Maike Sperk, Akos Vegvari, Flora Mikaeloff, Katie Healy, Jan Vesterbacka, Piotr Nowak, Anders Sonnerborg, Ujjwal Neogi
Summary: This study identified HIF signaling and glycolysis as specific traits of the elite controller phenotype in HIV-1 infection. The activation of the HIF signaling pathway was observed in male elite controllers, along with elevated intracellular glucose levels. These findings provide new insights into the natural control of HIV-1.
Article
Biology
Flora Mikaeloff, Sara Svensson Akusjarvi, George Mondinde Ikomey, Shuba Krishnan, Maike Sperk, Soham Gupta, Gustavo Daniel Vega Magdaleno, Alejandra Escos, Emilia Lyonga, Marie Claire Okomo, Claude Tayou Tagne, Hemalatha Babu, Christian L. Lorson, Akos Vegvari, Akhil C. Banerjea, Julianna Kele, Luke Elizabeth Hanna, Kamal Singh, Joao Pedro de Magalhaes, Rui Benfeitas, Ujjwal Neogi
Summary: This study utilized untargeted and targeted LC-MS/MS-based plasma metabolic profiling to identify dysregulated metabolism in individuals living with HIV, including altered glutaminolysis. The study also found decreased levels of neurosteroids in HIV-infected individuals, suggesting a potential link between HIV and neurological impairment.
COMMUNICATIONS BIOLOGY
(2022)
Article
Biology
Ujjwal Neogi, Nazif Elaldi, Sofia Appelberg, Anoop Ambikan, Emma Kennedy, Stuart Dowall, Binnur K. Bagci, Soham Gupta, Jimmy E. Rodriguez, Sara Svensson-Akusjarvi, Vanessa Monteil, Akos Vegvari, Rui Benfeitas, Akhil Banerjea, Friedemann Weber, Roger Hewson, Ali Mirazimi
Summary: Through system biology analysis of CCHF patients and cell infection models, this study found that CCHFV infection causes host metabolic reprogramming and negative coordination with biological signaling systems. Blocking key metabolic pathways can inhibit viral replication. Interferon-mediated antiviral mechanisms play an important role at the system level and during viral replication.
Article
Medicine, Research & Experimental
Friederike Praus, Axel Kuenstner, Thorben Sauer, Michael Kohl, Katharina Kern, Steffen Deichmann, Akos Vegvari, Tobias Keck, Hauke Busch, Jens K. K. Habermann, Timo Gemoll
Summary: This study integrated genomic, transcriptomic, and proteomic analyses to identify significant differences in expression during colorectal cancer progression. The results highlight the importance of tumor heterogeneity and individual differences for clinical studies, and may accelerate the implementation of precision oncology in the future.
JOURNAL OF TRANSLATIONAL MEDICINE
(2023)
Article
Infectious Diseases
Rafael Cena-Diez, Aswathy Narayanan, Shilpa Ray, Maarten van de Klundert, Jimmy E. Rodriguez, Johan Nilvebrant, Per-Ake Nygren, Akos Vegvari, Robert van Domselaar, Anders Sonnerborg
Summary: WC-am, a dipeptide, has been found to be increased in elite controllers, who can control HIV-1 infection. This study aimed to evaluate the anti-HIV-1 activity and mechanism of action of WC-am. The results showed that WC-am binds to the CD4 binding pocket of HIV-1 gp120, blocking its binding to host cell receptors, and inhibits HIV-1 replication by affecting reverse transcription.
INTERNATIONAL JOURNAL OF ANTIMICROBIAL AGENTS
(2023)
Article
Immunology
Yu-Ching Su, Mahendar Kadari, Megan L. L. Straw, Martina Janouskova, Sandra Jonsson, Oskar Thofte, Farshid Jalalvand, Erika Matuschek, Linda Sandblad, Akos Vegvari, Roman A. A. Zubarev, Kristian Riesbeck
Summary: Non-typeable Haemophilus influenzae (NTHi) is a Gram-negative human pathogen that causes various airway diseases. The outer membrane protein P5 contributes to bacterial serum resistance and also plays a critical role in maintaining bacterial outer membrane integrity and protein composition.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2023)
Article
Immunology
Abdul Muktadir Shafi, Akos Vegvari, Roman A. Zubarev, Carlos Penha-Goncalves
Summary: This study reveals the important role of the STING-INFb-CXCL10 pathway in brain microvascular endothelial cells (BECs) in the development of cerebral malaria (CM). Activation of this pathway leads to immunoproteasome activation, enhanced antigen presentation, and impairment of endothelial barrier function. These findings provide insights into the pathogenesis of CM and its lethality.
FRONTIERS IN IMMUNOLOGY
(2023)
Article
Medicine, Research & Experimental
Huqiao Luo, Vilma Urbonaviciute, Amir Ata Saei, Hezheng Lyu, Massimiliano Gaetani, Akos Vegvari, Yanpeng Li, Roman A. Zubarev, Rikard Holmdahl
Summary: Mutations in NCF1 gene that cause low production of ROS are strongly linked to systemic lupus erythematosus. The deficiency of ROS in plasmacytoid dendritic cells (pDCs) exacerbates lupus development by promoting pDC accumulation in multiple organs, elevating IFN-alpha levels, and activating IFN-stimulated genes. Mechanistic studies show that ROS deficiency enhances pDC generation and migration, which further augment type I IFN responses. Restoring NOX2-derived ROS specifically in pDCs protects against lupus by suppressing these pathways.
Article
Engineering, Biomedical
Milan Finn Wesseler, Nayere Taebnia, Sean Harrison, Sonia Youhanna, Lena C. Preiss, Aurino M. Kemas, Akos Vegvari, Jaroslav Mokry, Gareth J. Sullivan, Volker M. Lauschke, Niels B. Larsen
Summary: This study presents an accurate liver microphysiological system (MPS) created through engineering of 3D printed hydrogel chips with synthetic vasculature channels. The MPS allows the long-term culture of human liver cells and demonstrates improved molecular phenotypes compared to other 3D culture methods. The platform also successfully generates physiologically relevant oxygen gradients and shows zonation-specific toxicity patterns.
ACTA BIOMATERIALIA
(2023)
Article
Biochemistry & Molecular Biology
Christian M. Beusch, Oscar E. Simonson, Johan O. Wedin, Pierre Sabatier, Ulrika Felldin, Sandeep Kadekar, Cecilia Osterholm, Akos Vegvari, Roman A. Zubarev, Karin Fromell, Bo Nilson, Stefan James, Elisabeth Stahle, Karl-Henrik Grinnemo, Sergey Rodin
Summary: By comparing the extracellular matrix proteins in valve tissues from patients with isolated aortic valve degeneration, the study identified differences in the molecular cues and aetiologies between patients with tricuspid and bicuspid aortic valves, suggesting the need for different treatments and providing insights into the molecular basis of the condition.
CELLULAR AND MOLECULAR LIFE SCIENCES
(2023)
Article
Dentistry, Oral Surgery & Medicine
Alfredo Torres, M. Angelica Michea, Akos Vegvari, Marion Arce, Alicia Morales, Elias Lanyon, Marcela Alcota, Camila Fuentes, Rolando Vernal, Mauricio Budini, Roman A. Zubarev, Fermin E. Gonzalez
Summary: The study aimed to explore the qualitative and quantitative differences in gingival crevicular fluid (GCF) proteome of periodontitis patients during disease progression. It was found that the GCF proteome of progressive periodontitis was associated with metabolic processes, immune response, and cellular stress, while the proteome of stable periodontitis was mainly related to wound repair, cell death regulation, and autophagy.
JOURNAL OF PERIODONTAL RESEARCH
(2023)
Article
Biochemical Research Methods
Akos Vegvari, Xuepei Zhang, Roman A. Zubarev
Summary: This study successfully detected proteins from individual Escherichia coli bacteria for the first time, with validation of the findings through comparison with other samples and bulk proteomics data.
JOURNAL OF THE AMERICAN SOCIETY FOR MASS SPECTROMETRY
(2023)
Article
Neurosciences
Robert D. Hawkins, Lennart Brodin, Elvar Theodorsson, Akos Vegvari, Eric R. Kandel, Tomas Hokfelt
Summary: Neuropeptides are widely used as neurotransmitters in both vertebrates and invertebrates, but the complexity of the vertebrate nervous system has hindered the understanding of their functions as transmitters. The marine mollusk Aplysia, with its simpler nervous system and large identified neurons, offers advantages for studying neuropeptides' roles in behavior. By observing immunoreactivity in individual neurons in adult Aplysia, we have discovered potential unknown peptides involved in behavior. Furthermore, our studies have revealed a high degree of colocalization of different neuropeptides in individual neurons, suggesting complex interactions and functions.
Article
Nanoscience & Nanotechnology
Shahriar Sharifi, Amir Ata Saei, Hassan Gharibi, Nouf N. Mahmoud, Shannon Harkins, Naruphorn Dararatana, Erika M. Lisabeth, Vahid Serpooshan, Akos Vegvari, Anna Moore, Morteza Mahmoudi
Summary: Albumin-based hydrogels, especially the non-immunogenic photocurable version described in the study, demonstrate excellent biocompatibility and anti-inflammatory properties, making them promising materials for biomedical applications. The modifications and cross-linking of methacrylated HSA were found to reduce macrophage adhesion and inflammatory pathways, with potential for use in passivation coatings and other biomedical applications. In vitro and Ex-Ovo assay results confirmed the biocompatibility and slight angiogenesis-modulating effects of the photocurable albumin hydrogels.
ACS APPLIED BIO MATERIALS
(2022)
Article
Virology
Sofia Appelberg, Lijo John, Norbert Pardi, Akos Vegvari, Sandor Bereczky, Gustaf Ahlen, Vanessa Monteil, Samir Abdurahman, Flora Mikaeloff, Mitchell Beattie, Ying Tam, Matti Sallberg, Ujjwal Neogi, Drew Weissman, Ali Mirazimi
Summary: Crimean-Congo hemorrhagic fever (CCHF), caused by CCHFV, is a prioritized disease. In this study, vaccination with mRNA-LNP encoding CCHFV proteins protected mice against lethal infection and induced robust immune responses. The vaccine also affected liver injury.
JOURNAL OF VIROLOGY
(2022)