期刊
PROTEOMICS
卷 12, 期 10, 页码 1519-1526出版社
WILEY
DOI: 10.1002/pmic.201100471
关键词
Biochemical methods; Cell signaling pathways; Membrane proteins; Protein-protein interactions; Technology
资金
- Canadian Foundation for Innovation (CFI)
- Canadian Institutes of Health Research (CIHR)
- Canadian Cancer Society Research Institute (CCSRI)
- Heart and Stroke Foundation
- Cystic Fibrosis Foundation
- Ontario Genomics Institute
- Novartis
Membrane-bound proteins are one of the most important protein types in the cell, and are involved in many major cell processes and signaling pathways. Most proteins, including those at membranes, must interact with other proteins to form complexes, which are essential for their function(s). In this review, we describe some of the major non-mass spectrometry-based methods and technologies used for the investigation of intracellular membrane protein complexes including Tango, fluorescence/bioluminescence resonance energy transfer (F/BRET), luminescence-based mammalian interactome mapping (LUMIER), protein-fragment complementation assay (PCA), and membrane yeast two-hybrid assay (MYTH). We highlight the advantages and drawbacks of these methods, describe recent studies utilizing these methods, and discuss some of the major findings in the study of membrane protein-based cell pathways.
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