Article
Biochemical Research Methods
Kevin Hooijschuur, Xiufen Liu, Anna Grootendorst, Ines Pieterman, Javier Sastre Torano
Summary: The use of mixed ion exchange particles and diatomite allows for high-capacity analysis of low-abundant compounds in CZE-DTIMS-MS, increasing sample injection volumes and flow rates.
Review
Chemistry, Analytical
Denisa Smolkova, Richard Cmelik, Jana Lavicka
Summary: Capillary electrophoresis is widely used for oligosaccharide and glycan analysis, but the low electrophoretic mobility and lack of chromophore or fluorophore in saccharide molecules require modification before separation and detection. This review summarizes the labeling strategies used for oligosaccharide/glycan analysis, categorized based on the labeling mechanism and saccharide molecule. In addition to the commonly used 8-aminopyrene-1,3,6-trisulfonic acid trisodium salt, less common and recently developed tags applicable in glycomics are also summarized.
TRAC-TRENDS IN ANALYTICAL CHEMISTRY
(2023)
Review
Biochemical Research Methods
Vaclav Kasicka
Summary: This review article provides a comprehensive survey of the developments and applications of high-performance capillary and microchip electromigration methods for peptide analysis, micropreparation, and physicochemical characterization. The article presents progress in the study of electromigration properties of peptides and various aspects of their analysis, as well as new developments in capillary electromigration methods and their applications. It covers a wide range of applications, including qualitative and quantitative analysis of peptides, peptide profiling, monitoring of reactions and physicochemical changes, amino acid and sequence analysis, separation of stereoisomers, and chiral analyses.
Review
Biochemical Research Methods
Vaclav Kasicka
Summary: The review comprehensively covers the developments and applications of high performance capillary and microchip electroseparation methods for peptide analysis and physicochemical characterization from 2019 to mid-2021. It highlights advancements in studying electromigration properties, analysis methodology improvements, and new applications such as micropreparative separations and determination of significant physicochemical parameters of peptides.
Article
Chemistry, Analytical
Theo Lienard-Mayor, Camille Bricteux, Amel Bendali, Nguyet-Thuy Tran, Arnaud Bruneel, Myriam Taverna, Thanh Duc Mai
Summary: In this study, a new microfluidic droplet platform called Lab-in-Droplet is presented for multistep glycoprotein sample treatment. The platform enables full automation, droplet sensing and heating, allowing for the realization of several advanced functions such as heating and mixing with organic solvents. The Lab-in-Droplet platform demonstrated superiority over the conventional batchwise protocol, with reduced reagent consumption, shorter processing time, improved glycan labeling yield, and maintained glycan separation profile.
ANALYTICA CHIMICA ACTA
(2022)
Article
Chemistry, Analytical
Cedric Delvaux, Marjorie Dauvin, Madeleine Boulanger, Loic Quinton, Dominique Mengin-Lecreulx, Bernard Joris, Edwin De Pauw, Johann Far
Summary: Peptidoglycan (PGN) is a crucial structure in bacterial cell walls, continuously synthesized and degraded during bacterial life cycle for growth and division. PGN fragments act as messenger molecules in bacterial cell wall stress response and triggering mammalian innate immune response. Bacteria modulate their PGN degradation or recognition by chemically modifying PGN, such as amidating the epsilon-carboxyl group of meso-diaminopimelic acid in the PGN peptide chain. The quantitation of PGN-derived peptides is challenging due to their hydrophilic nature, but capillary zone electrophoresis coupled with high-resolution mass spectrometry shows excellent repeatability in quantitation of PGN peptides and their derivatives in bacterial cytoplasmic extracts.
ANALYTICAL CHEMISTRY
(2021)
Article
Chemistry, Analytical
Amanda Helms, Edwin E. E. Escobar, Saulius Vainauskas, Christopher H. H. Taron, Jennifer S. S. Brodbelt
Summary: The complete characterization of O-glycopeptides, including identification of peptides, localization of glycosites, and mapping of glycans, has been a challenging task in O-glycoproteomics. This study successfully applied a strategy combining the use of O-glycoprotease IMPa and HCD-triggered UVPD to achieve the complete characterization of O-glycopeptides. This approach allowed the localization of multiple adjacent or proximal O-glycosites and the identification of a previously unknown glycosite on etanercept.
ANALYTICAL CHEMISTRY
(2023)
Review
Chemistry, Analytical
Vaclav Kasicka
Summary: This review article provides a comprehensive overview of the important developments and applications of capillary electromigration methods in the field of peptide mapping of proteins. It covers various aspects including sample preparation, enzymatic and chemical cleavage, separation and detection methods, as well as the wide range of applications of peptide mapping.
JOURNAL OF SEPARATION SCIENCE
(2022)
Article
Chemistry, Analytical
Erik Saparbaev, Andrei Zviagin, Oleg Boyarkin
Summary: We propose a universal approach for the quantitative identification of different isomeric biomolecules using infrared fragment cold ion spectroscopy. The method utilizes solvent molecules as natural tags, eliminating the need for any special compounds and allowing for the measurement of relative concentrations of isomers.
ANALYTICAL CHEMISTRY
(2022)
Article
Biochemistry & Molecular Biology
Emmanouil Mavrogeorgis, Harald Mischak, Agnieszka Latosinska, Justyna Siwy, Vera Jankowski, Joachim Jankowski
Summary: In recent years, CE-MS has been increasingly used in clinical research for chronic and age-associated diseases, allowing for comprehensive assessment of small molecular weight proteins and peptides. The study evaluated the analytical performance of CE-MS in terms of reproducibility, variability, and efficiency in peptide detection, showing high correlation among different runs and low variation in signal intensity. These findings support the value of CE-MS for clinical application.
Article
Chemistry, Analytical
Xiaomei Zhou, Woran Song, Milos V. Novotny, Stephen C. Jacobson
Summary: Structural isomers of sialylated N-glycans contribute to the diversity of the N-glycome and can be easily distinguished by mass spectrometry. An improved alkylamidation method with a delactonization step was used to enrich and stabilize sialylated N-glycans for structural characterization.
JOURNAL OF SEPARATION SCIENCE
(2022)
Review
Chemistry, Analytical
Blanca H. Lapizco-Encinas, Y. Victoria Zhang, Putuma P. Gqamana, Jana Lavicka, Frantisek Foret
Summary: Capillary electrophoresis (CE) is a powerful separation technique that offers high resolution, great sensitivity, and short processing times. When combined with mass spectrometry (MS), CE-MS systems provide accurate characterization of ionized species and significantly enhance the capabilities of MS. This review article introduces the analytical potential of CE-MS systems and discusses recent advances and future developments.
TRAC-TRENDS IN ANALYTICAL CHEMISTRY
(2023)
Review
Chemistry, Analytical
Theo Lienard-Mayor, Myriam Taverna, Stephanie Descroix, Thanh Duc Mai
Summary: This study provides a comprehensive overview of different strategies for combining droplet-based sample handling and preparation with electrophoretic separation. It focuses on three principal interfacing strategies, including droplets in immiscible phases, digital microfluidics with electrowetting-on-dielectric principle, and inkjet droplet generation. Different instrumental developments, pros and cons of designs, and application demonstrations of droplet-interfaced electrokinetic strategies are discussed.
ANALYTICA CHIMICA ACTA
(2021)
Review
Chemistry, Multidisciplinary
Yulun Wu, Huangxu Li, Tiancheng Liu, Ming Xu
Summary: This review comprehensively examines the use of proteins and their subunits in addressing engineering issues in various rechargeable batteries. The latest advances in electrode, electrolyte, separator, and other components are discussed, with a particular emphasis on the impact of biomolecules on electrochemical properties.
ADVANCED MATERIALS
(2023)
Article
Oncology
Ayman Bannaga, Jochen Metzger, Torsten Voigtlaender, Martin Pejchinovski, Maria Frantzi, Thorsten Book, Sean James, Kishore Gopalakrishnan, Harald Mischak, Michael P. Manns, Ramesh P. Arasaradnam
Summary: Capillary electrophoresis mass spectrometry was used in this study to identify 31 urinary peptides significantly associated with hepatocellular carcinoma diagnosis and prognosis. These peptides were found to predict proteases involved in the development of the cancer, offering a potential noninvasive tool for diagnosis and prognosis. Immunohistochemistry verification of the identified proteases in liver tissues further supported their potential roles in liver fibrosis and cancer formation.
Article
Chemistry, Analytical
Nadhiratul-Farihin Semail, Aemi Syazwani Abdul Keyon, Bahruddin Saad, Sazlinda Kamaruzaman, Nur Nadhirah Mohamad Zain, Vuanghao Lim, Mazidatulakmam Miskam, Wan Nazwanie Wan Abdullah, Noorfatimah Yahaya, David D. Y. Chen
Summary: A dynamic pH junction was utilized in capillary electrophoresis to preconcentrate, separate, and determine trace amounts of sulfonamide antibiotics in milk and yoghurt samples. The method demonstrated good linearity, low limits of detection, and acceptable analytes recovery, with quantification limits below the maximum residue limit approved by the European Union. Sensitivity enhancement factors of up to 129 were achieved with the optimized dynamic pH junction using CE with a diode array detector.
Article
Biochemical Research Methods
Zi-Ao Huang, Jiahua Tan, Yueyang Li, Siyu Miao, Kymora B. Scotland, Ben H. Chew, Dirk Lange, David D. Y. Chen
Summary: Migration time correction is crucial in CE-based metabolomics, especially for dual pressure CE. We proposed a new correction method and developed a script tool for migration time correction and peak alignment. A case study demonstrated the effectiveness of this method.
Article
Chemistry, Applied
Noorfatimah Yahaya, Zi-Ao Huang, Binjun Yan, David D. Y. Chen
Summary: The method presented in this study allows for the sensitive determination of bisphenol A and its analogues in bottled tea beverages at the ng/mL level by utilizing dynamic pH junction. The optimized analyte focusing results in enhanced signal detection, with good linearity, low detection limits, high analyte recovery, and promising practical applications.
Article
Chemistry, Multidisciplinary
Dan Cao, Min Liu, Yung-Chih Su, Zehui Yang, Wentao Bi, David Da Yong Chen
Summary: This study investigates the mechanism and influencing factors of dyeing polyester fabrics (PETFs) using deep eutectic solvents (DESs). The research finds that anthraquinone dyes have different interactions with DESs, and successful dyeing of PETF depends on strengthening the interactions between PETF and the dyes. The type of DES, hydrogen bond acceptor-hydrogen bond donor ratio, dye concentration, dyeing temperature, and dyeing time also affect the adsorption and dyeing efficiency.
SUSTAINABLE CHEMISTRY AND PHARMACY
(2022)
Article
Biochemical Research Methods
Liping Xu, Kai Zhang, Xin Geng, Hongli Li, David Da Yong Chen
Summary: A modified method was used for analyzing human metabolites in exhaled breath, providing a more complete picture of the human breath metabolome. The method allows rapid and direct analysis of breath samples with different status, featuring simple operation and metabolite identification, requiring little or no sample preparation.
RAPID COMMUNICATIONS IN MASS SPECTROMETRY
(2022)
Article
Biochemical Research Methods
Rongfan Ren, Hongli Li, Qing Jiang, Xing Wang, David Da Yong Chen
Summary: Characterization of structural isomers of bioactive molecules is important but challenging due to their similar structures. This study demonstrated a simple and robust method using in situ methylation reaction with TMAH and DART ion source to characterize saponin isomers. The method allowed for the identification of ginsenosides in Panax ginseng without the need for chromatographic separation or offline sample pretreatment.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2023)
Article
Biochemical Research Methods
Min Liu, Simin Wang, Wuxia Ge, Wentao Bi, David Da Yong Chen
Summary: This study systematically investigated the influence of host-guest interactions on the analytical performance of direct analysis in real time mass spectrometry (DART-MS) by studying the interactions between cyclodextrins (CDs) and Sudan dyes. The results showed that the host-guest interaction between CDs and Sudan dyes affected the quantitative analysis of the target compounds by lowering the signal intensity. Furthermore, the addition of organic solvents weakened the host-guest interaction and improved the signal intensity in DART-MS, particularly in solid-phase microextraction (SPME).
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2023)
Article
Chemistry, Analytical
Rongfan Ren, Minghui Yuan, Hongli Li, David Da Yong Chen
Summary: Carbohydrates are essential for energy supply and biological functions, but identifying and distinguishing their isomers has been challenging due to their similar structures and possible glycosidic linkages. This study developed an ionization tandem mass spectrometry method to characterize disaccharide isomers by in situ methylation. The method allowed for immediate methylation of hydroxyl groups on disaccharides, enabling their ionization and distinction through mass spectrometry. This approach was used to directly identify disaccharide isomers in real commercial products without complex pretreatment or chromatographic separation.
ANALYTICAL CHEMISTRY
(2023)
Article
Chemistry, Applied
Min Liu, Simin Wang, Wentao Bi, David Da Yong Chen
Summary: Deep eutectic systems (DESys) formed by hydrogen bond acceptor (HBA) and polysaccharide (hydrogen bond donor, HBD) in water can enhance the extraction efficiency and retain the physicochemical properties of polysaccharides. This method simplifies the recovery and purification process of polysaccharides.
Article
Biochemical Research Methods
Yuanli Dai, Chang Sun, Yunhe Yang, Shuanghao Wang, Huihui Li, David Da Yong Chen
Summary: The thermodynamic properties of molecular recognition in host-guest inclusion complexes were studied using Taylor dispersion analysis (TDA). TDA provided rapid and convergent results for the binding properties of cyclodextrins (CDs) and their derivatives with physiologically active substances. The TDA results were compared with those obtained by nuclear magnetic resonance and affinity capillary electrophoresis (ACE), showing slightly lower binding constants from ACE.
Article
Chemistry, Applied
Zhixin Xiao, Min Liu, Wentao Bi, David Da Yong Chen
Summary: In-depth studies of extraction mechanism using deep eutectic solvents (DES) and ionic liquids (IL) reveal similarities between the two systems. New applications of ILs and DES in the extraction of nutritional natural products are presented. The study systematically investigates the extraction behavior of ChCl and [HMIm][Cl] in DES and IL, respectively, for target compound extraction from Moringa oleifera leaves. The results show that both extraction methods are based on the formation of a DESys, which can be improved by the formation of a new IL deep eutectic system based on hydrogen bond interaction.
Article
Chemistry, Analytical
Xin Geng, Kai Zhang, Hongli Li, David Da Yong Chen
Summary: A robust ambient ionization mass spectrometry method using a modified direct analysis in real time (DART) ion source was developed for online analysis of breath volatiles. The method is fast, simple, versatile, and has the potential to evaluate the true state of human exhaled breath.
Article
Chemistry, Analytical
Binjun Yan, Mengtian Shi, Siyu Cai, Yuan Su, Renhui Chen, Chiyuan Huang, David Da Yong Chen
Summary: Proteomics is essential for understanding biology and diseases, with liquid chromatography-tandem mass spectrometry (LC-MS/MS) being a widely used platform. Data-independent acquisition (DIA) improves the reproducibility of LC-MS/MS in proteomics research. However, existing DIA data processing tools sometimes lead to significant deviations in quantification. This study presents a cross-run ion selection and peak-picking (CRISP) tool that takes advantage of the consistency of DIA data across runs to filter out interfering signals, resulting in improved quantification accuracy.
ANALYTICAL CHEMISTRY
(2023)
Article
Environmental Sciences
Pan Yan, Lingfei Kong, Tuo Qin, Zhonggeng Luo, Xiaona Zhang, Cai Tie
Summary: Using ultra-performance liquid chromatography-high-resolution mass spectrometry (UPLC-HRMS), this study identified the monohydroxyl metabolites of naphthalene, phenanthrene, pyrene, and 9-fluorenone in the urine of low-dose PAH-exposed individuals. The concentrations of these metabolites were monitored for 15 consecutive days, and the results showed that they were strongly correlated with their parent PAH exposure and may serve as potential biomarkers of PAH internal exposure at low doses. This study provides methodological and data support for further health risk studies involving internal exposure to PAHs and other organic pollutants.
ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH
(2023)
Article
Chemistry, Applied
Xiwang Cao, Min Liu, Wentao Bi, Jun Li, David Da Yong Chen
Summary: This study investigates the carboxylation of cellulose particles using a deep eutectic solvent containing carboxylic acid. The effects of key factors on cellulose carboxylation are systematically studied, and the reaction mechanism and the influence of the solvent on the structure and properties of cellulose are examined. The results show that the type of carboxylic acid, cellulose particle size, and pretreatment methods significantly affect the carboxylation efficiency and properties of cellulose. The study also demonstrates the potential application of carboxyl cellulose in pollutant adsorption.
CARBOHYDRATE POLYMER TECHNOLOGIES AND APPLICATIONS
(2022)