期刊
PROTEOMICS
卷 12, 期 22, 页码 3386-3392出版社
WILEY
DOI: 10.1002/pmic.201100642
关键词
Animal proteomics; Hypothalamus; MS; Protein biomarker; Proteomics methods
资金
- European Innovative Medicines Initiative NEWMEDS
- Autism Speaks
- BBSRC [BB/F015364/1] Funding Source: UKRI
- Biotechnology and Biological Sciences Research Council [BB/F015364/1] Funding Source: researchfish
- British Heart Foundation [FS/09/029/27902] Funding Source: researchfish
- Medical Research Council [G0600717B] Funding Source: researchfish
Studies of neuronal, endocrine, and metabolic disorders would be facilitated by characterization of the hypothalamus proteome. Protein extracts prepared from 16 whole rat hypothalami were measured by data-independent label-free nano LC-MS/MS. Peptide features were detected, aligned, and searched against a rat Swiss-Prot database using ProteinLynx Global Server v.2.5. The final combined dataset comprised 21 455 peptides, corresponding to 622 unique proteins, each identified by a minimum of two distinct peptides. The majority of the proteins (69%) were cytosolic, and 16% were membrane proteins. Important proteins involved in neurological and synaptic function were identified including several members of the Ras-related protein family and proteins involved in glutamate biosynthesis.
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