期刊
PROTEOMICS
卷 12, 期 15-16, 页码 2539-2546出版社
WILEY-BLACKWELL
DOI: 10.1002/pmic.201200070
关键词
Cell biology; Free-flow electrophoresis; Simulated microgravity; Thyroid cancer
资金
- European Space Agency [CORA-GBF-2010 - 202]
- German Space Agency DLR [50WB1124]
- ETH Zurich, Switzerland
- Ministerium fur Innovation, Wissenschaft, Forschung und Technologie des Landes Nordrhein-Westfalen
- Bundesministerium fur Bildung und Forschung
Many cancer cells show unique protein expression patterns. We used proteome technology including MS, free flow isoelectric focusing and Western blotting to determine current concentrations of metabolic enzymes in healthy and malignant human thyroid cells. Three different types of human thyroid cells were investigated after they had been cultured under equal conditions. MS revealed high quantities of glycolytic enzymes and moderate quantities of citric acid cycle enzymes in malignant FTC-133 cells with abnormal LDH B-chains, high quantities of glycolytic enzymes and marginal quantities of citric acid cycle enzymes in normal HTU-5 cells, and low quantities of glycolytic enzymes and marginal quantities of citrate cycle enzymes in malignant CGTH-W1 cells with abnormal LDH A-chains. When an alteration of gene expression activity was challenged physically by removing gravity forces, the concentrations of various glycolytic enzymes were changed in normal and malignant thyroid cells. However, the changes varied among the different cell types. Different cellular alignment of the enzymes could be one reason for the cell type-specific behavior as demonstrated by histological analysis of alpha-enolase.
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