期刊
PROTEOMICS
卷 12, 期 11, 页码 1879-1882出版社
WILEY
DOI: 10.1002/pmic.201100576
关键词
Animal proteomics; Deyolking; Fractionation; Gene ontology; Whole proteome; Zebrafish embryo
资金
- Agency for Science, Technology and Research (AstarSTAR), Singapore
The proteome of zebrafish, Danio rerio, embryos has not been studied in great detail mainly due to the presence of high abundance yolk proteins in embryos. Here we report the highest number of the zebrafish embryo proteins identified so far to our knowledge, through a combination of a protein-level fractionation approach (1D SDS-PAGE) and two different peptide-level fractionation approaches (IEF and strong anion exchange (SAX)) of deyolked zebrafish embryos followed by LC-MS/MS. We detected 5267 proteins in total of which 3464 proteins were identified with at least two peptides (less than 1% peptide false discovery rate). The analysis of proteome coverage from each method showed that 56% of detected proteins were common to all approaches and 95% of the detected proteome was obtained from 1D SDS-PAGE approach alone. Bioinformatics analysis of the detected proteome demonstrated that nucleocytoplasmic transport (biological process) and ribosomal proteins (cellular component) were the most over-represented proteins, whereas cellcell signaling (biological process) and extracellular space proteins (cellular component) were the most under-represented proteins in the identified proteome.
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