期刊
PROTEOMICS
卷 11, 期 3, 页码 429-439出版社
WILEY
DOI: 10.1002/pmic.200900819
关键词
Cell biology; Epithelial-mesenchymal transition; Heterogeneous nuclear ribonucleoprotein K; Lung cancer; Subcellular proteomics
资金
- Chang-Jiang Scholars Program, 211 Projects, National Basic Research Program of China (973 Program)
- State Key Laboratory of Respiratory Disease
- Science and Technology Planning Projects of Guangzhou and Guangdong, China
Subcellular proteomics was used to compare the protein profiles between human lung adenocarcinoma A549 cells and human bronchial epithelial (HBE) cells. In total, 106 differential proteins were identified and the altered expression levels of partial identified proteins were confirmed by Western blot analysis. Importantly, pathway analysis and biological validation revealed epithelial mesenchymal transition (EMT) phenotype shift in A549 cells as compared with HBE cells. The EMT phenotype of A549 cells can be increased by self-producing TGF-beta 1 and significantly decreased by silencing heterogeneous nuclear ribonucleoprotein (hnRNPK) expression. As EMT has been considered as an important event during malignant tumor progression and metastasis, investigating EMT and deciphering the related pathways may lead to more efficient strategies to fight lung cancer progression. By integrating the subcellular proteomic data with EMT-related functional studies, we revealed new insights into the EMT progress of lung carcinogenesis, providing clues for further investigations on the discovery of potential therapeutic targets.
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