期刊
PROTEOMICS
卷 11, 期 23, 页码 4503-4513出版社
WILEY-BLACKWELL
DOI: 10.1002/pmic.201100101
关键词
C8-modified interior pore-walls; Magnetic mesoporous microspheres; MALDI-TOF MS; Mouse brain; Nanoproteomics; Peptidome analysis
资金
- National Natural Science Foundation of China [21075022, 20875017, 30873132, 21105016]
- National Basic Research Priorities Program [2007CB914100/3]
- Research Fund for the Doctoral Program of Higher Education of China [20100071120053]
- Shanghai Municipal Natural Science Foundation [11ZR1403200]
- Technological Innovation Program of Shanghai [09JC1401100]
- Shanghai Leading Academic Discipline Project [B109]
In this paper, magnetic mesoporous silica microspheres with C8-modified interior pore-walls were prepared through a facile one-pot sol-gel coating strategy, and were successfully applied for selective enrichment of endogenous peptides in mouse brain for peptidome analysis. Through the one-pot sol-gel approach with surfactant (CTAB) as a template, tetraethyl orthosilicate (TEOS) and n-ctyltriethoxysilane (C8TEOS) as the precursors, C8-modified magnetic mesoporous microspheres (C8-Fe3O4@mSiO(2)) consisting magnetic core and mesoporous silica shell with C8-groups exposed in the mesopore channels were synthesized. The obtained microspheres possess highly open mesopores of 3.4 nm, high surface area (162.5 m(2)/g), large pore volume (0.17 cm(3)/g), excellent magnetic responsivity (56.3 emu/g) and good dispersibility in aqueous solution. Based on the abundant surface silanol groups, functional C8 groups and the strong magnetic responsivity of the core-shell C8-Fe3O4@mSiO(2) microspheres, efficient and fast enrichment of peptides was achieved. Additionally, the C8-Fe3O4@mSiO(2) microspheres exhibit excellent performance in selective enrichment of endogenous peptides from complex samples that are consist of peptides, large proteins and other compounds, including human serum and mouse brain followed by automated nano-LC-ESI-MS/ MS analysis. These results indicate C8-Fe3O4@mSiO(2) microspheres would be a potential candidate for endogenous peptides enrichment and biomarkers discovery in peptidome analysis.
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