Review
Oncology
Timothy I. Shaw, Bi Zhao, Yuxin Li, Hong Wang, Liang Wang, Brandon Manley, Paul A. Stewart, Aleksandra Karolak
Summary: This article discusses the role of cancer-specific alternatively spliced events (ASE) in cancer pathogenesis and proposes an integrated multi-omics strategy to mine ASE as potential targets for therapeutic development. The article provides an overview of current multi-omics strategies in characterizing ASEs using transcriptome, proteome, and protein structure prediction algorithms, discusses the limitations and knowledge gaps associated with each technology and informatics analytics, and explores future directions for the full integration of multi-omics data in ASE target discovery.
FRONTIERS IN ONCOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Georgii Dolgalev, Ekaterina Poverennaya
Summary: In the past 8 years, studies have revealed the widespread occurrence of isoform switching in human cancers with hundreds to thousands of events per cancer type. However, the relationship between changes in transcript usage and transcript expression has not been adequately explored. In this article, the authors use a state-of-the-art tool, SatuRn, to detect isoform switching events in 12 cancer types and analyze the relationship between transcript usage and expression on a global scale. The results suggest that this quantitative information can effectively prioritize isoform switching events for further analysis.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Cell Biology
Weidi Wang, Yucan Chen, Jingjing Zhao, Liang Chen, Weichen Song, Li Li, Guan Ning Lin
Summary: The study explored the protein-protein interaction of NSD2 and its role in WHS and myeloma. Through immunoprecipitation mass spectrometry analysis, 890 isoform-specific PPI partners were identified, further divided into four categories of exon-specific interactome. The research revealed the diverse roles of NSD2 in RNA splicing processes.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Review
Biochemistry & Molecular Biology
Ricardo De Paoli-Iseppi, Josie Gleeson, Michael B. Clark
Summary: Alternative splicing of RNA is a key mechanism leading to the expression of multiple transcript isoforms from a single gene, increasing the complexity of the transcriptome and proteome. Long-read sequencing technologies can address the limitations of short-read sequencing in resolving complex isoforms and distinguish changes that may impact RNA regulation or protein function. Integration of long-read sequencing with other technologies allows for detailed profiling of alternative splicing at the isoform level and determination of the impact of genetic variation on isoform expression.
FRONTIERS IN MOLECULAR BIOSCIENCES
(2021)
Article
Biology
Markus J. Sommer, Sooyoung Cha, Ales Varabyou, Natalia Rincon, Sukhwan Park, Ilia Minkin, Mihaela Pertea, Martin Steinegger, Steven L. Salzberg
Summary: The development of three-dimensional protein structure prediction methods has provided new opportunities for research on genomes and proteomes. By utilizing computational predictions of protein structures, it is possible to identify the functional protein product among multiple gene isoforms. In this study, we evaluated over 230,000 isoforms of human protein-coding genes using protein structure predictions, and identified several isoforms with more confidently predicted structures and potentially superior function compared to the canonical isoforms in the latest human gene database. We demonstrated the potential of protein structure prediction as a genome annotation tool and provided a resource of protein structures for better understanding the function of human genes and their isoforms.
Editorial Material
Cell Biology
Brett W. Stringer, Laura Gantley, Simon J. Conn
Summary: The research on circular RNA (circRNA) in mainstream science has made significant progress in understanding their biogenesis, interactome, and functions in diverse fields and human diseases. Most studies have focused on their interaction with microRNAs, but it is time for another revolution in circRNA research to uncover functions beyond their binding ability to miRNAs.
Article
Biochemistry & Molecular Biology
Chiharu Hidaka, Shinichi Mitsui, Yoij Osako, Kou Takahashi, Kenjiro Tanaka, Kazunari Yuri
Summary: This study investigated the expression patterns of Sez6 splicing isoforms in the mouse brain and cultured neurons, revealing brain area-specific expression. Furthermore, neuronal activation and certain drugs were found to modulate the splicing patterns of Sez6.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2022)
Article
Marine & Freshwater Biology
Yide He, Rongwen Zhu, Yujie Cai, Yiqun Zhang, Yunhai Zhang, Shunlong Pan, Rudolf J. Schneider, Yongjun Zhang
Summary: In this study, the degradation pathway of Nebivolol (NEB) under UV radiation was investigated, and zebrafish embryos/larvae were used to evaluate the median lethal concentration (LC50) of NEB. Transcriptomic analysis and protein biomarker analysis revealed that UV radiation could reduce the toxic effects of NEB on the early development of zebrafish by affecting genes and pathways associated with antioxidant, nervous, and immune systems.
AQUATIC TOXICOLOGY
(2022)
Article
Immunology
Bowen Ni, Guanglong Huang, Runwei Yang, Ziyu Wang, Haimin Song, Kaishu Li, Yunxiao Zhang, Kezhi Wu, Guangwei Shi, Xiran Wang, Jie Shen, Yawei Liu
Summary: This study identified a novel membrane protein isoform, MS4A7-s, generated by alternative splicing in the glioblastoma (GBM) tumor microenvironment (TME). MS4A7-s is a specific M2 plasma membrane protein that plays a pro-oncogenic role in GBM TME. Higher expression of MS4A7-s is associated with poor prognosis in GBM patients. Furthermore, MS4A7-s promotes activation of the PI3K/AKT/GSK3 beta pathway in glioma-associated macrophages (GAMs), leading to M2 polarization and malignant progression of GBM.
JOURNAL OF NEUROINFLAMMATION
(2023)
Article
Genetics & Heredity
Elizabeth Tseng, Jason G. Underwood, Brandon D. Evans Hutzenbiler, Shawn Trojahn, Brewster Kingham, Olga Shevchenko, Erin Bernberg, Michelle Vierra, Charles T. Robbins, Heiko T. Jansen, Joanna L. Kelley
Summary: Understanding the hibernation process in brown bears provides valuable insights into human diseases. This study used full-length RNA sequencing to identify differential isoform usage during hibernation and active seasons in adipose, skeletal muscle, and liver tissues of brown bears. The results showed a high level of differential isoform expression in adipose tissue, indicating the importance of isoform expression in hibernation physiology. This research highlights the significance of studying isoforms and their role in hibernation biology.
G3-GENES GENOMES GENETICS
(2022)
Article
Biotechnology & Applied Microbiology
Zhonghui Xu, John Platig, Sool Lee, Adel Boueiz, Rob Chase, Dhawal Jain, Andrew Gregory, Rahul Suryadevara, Seth Berman, Russell Bowler, Craig P. Hersh, Alain Laederach, Peter J. Castaldi
Summary: Cigarette smoking induces significant transcriptomic and systemic inflammatory responses, affecting gene expression, alternative isoform regulation, and exon usage. Lengthening of 3' UTRs associated with smoking suggests potential biomarkers and therapeutic targets for smoking-related diseases, emphasizing the importance of further research in this area.
Review
Cell Biology
Geneva R. LaForce, Polyxeni Philippidou, Ashleigh E. Schaffer
Summary: Balanced mRNA isoform diversity and abundance are regulated spatially and temporally during cellular differentiation. Neurons have unique cellular characteristics and diversity among subtypes, which are established by neuron-specific gene expression programs. Genes with neuron-specific alternative processing play important roles in neuronal functions. Understanding neuronal RNA processing mechanisms is essential for studying neuronal homeostasis and disease predisposition.
WILEY INTERDISCIPLINARY REVIEWS-RNA
(2023)
Article
Oncology
Xiangyu Li, Woonghee Kim, Muhammad Arif, Chunxia Gao, Andreas Hober, David Kotol, Linnea Strandberg, Bjorn Forsstrom, Asa Sivertsson, Per Oksvold, Hasan Turkez, Morten Grotli, Yusuke Sato, Haruki Kume, Seishi Ogawa, Jan Boren, Jens Nielsen, Mathias Uhlen, Cheng Zhang, Adil Mardinoglu
Summary: Pyruvate kinase muscle type (PKM) is a key enzyme in glycolysis and plays an important role in cancer. The association between PKM expression and the survival outcome of patients with different cancers is controversial. System biology methods were employed to reveal the prognostic value and potential biological functions of PKM transcripts in different human cancers.
Article
Genetics & Heredity
Etienne Boileau, Xue Li, Isabel S. Naarmann-de Vries, Christian Becker, Ramona Casper, Janine Altmueller, Florian Leuschner, Christoph Dieterich
Summary: The study introduces a software suite called Single-cell Nanopore Spatial Transcriptomics (scNaST), which facilitates the analysis of spatial gene expression from second- and third-generation sequencing. It allows the generation of a full-length near-single-cell transcriptional landscape of the tissue microenvironment. The researchers demonstrated the workflow using sections of the mouse heart and identified novel modes of transcription associated with the infarct area. These findings have important implications for the development of therapeutic strategies for heart disease.
FRONTIERS IN GENETICS
(2022)
Article
Medical Laboratory Technology
Tianyang Wang, Ping Li, Xiangyu Meng, Jinling Zhang, Qi Liu, Cuicui Jia, Nana Meng, Kunjie Zhu, Dan Lv, Lei Sun, Tinghuizi Shang, Yan Lin, Weipan Niu, Song Lin
Summary: This study used an integrated multi-omic approach to differentiate healthy subjects from schizophrenia patients. By combining metabolomic and transcriptomic platforms, 86 differential metabolites and 189 differential expression genes were identified as potential biomarkers for schizophrenia. The functional relationships between metabolites and genes were further studied, providing more precise information on the mechanism and diagnostic evidence of schizophrenia. The establishment of characteristic diagnostic formulas enhances the predictive ability of the diagnostic spectrums.
CLINICA CHIMICA ACTA
(2022)