期刊
PROTEOMICS
卷 11, 期 14, 页码 2881-2890出版社
WILEY
DOI: 10.1002/pmic.201000636
关键词
2-D DIGE; Biomedicine; Cartilage; Kashin-Beck disease
资金
- National Natural Scientific Foundation of China [30630058, 30972556]
- Specialized Research Fund for the Doctoral Program of Higher Education of China [20090201110049]
- Project of Sino-Finnish Scientific and Technological Cooperation [2006DFA33610]
- Academy of Finland [128117]
Kashin-Beck disease (KBD) is a chronic endemic osteochondropathy with unclear pathogenesis. It is a degenerative disease similar to osteoarthritis, but with different manifestations of cartilage damage. The aim of this investigation was to show the protein changes in KBD cartilage and to identify the candidate proteins in order to understand the pathogenesis of the disease. Proteins were extracted from the media of primary cell cultures of KBD and normal chondrocytes, and separated by two-dimensional fluorescence difference gel electrophoresis (2-D DIGE). MALDI-TOF/TOF analysis revealed statistically significant differences in 27 proteins from KBD chondrocyte cultures, which consisted of 17 up-regulated and ten down-regulated proteins. The results were further validated by Western blot analysis. The proteins identified are mainly involved in cellular redox homeostasis and stress response (MnSOD, Hsp27, Peroxiredoxin-1, and Cofilin-1), glycolysis (PGK-1, PGM-1, alpha-enolase), and cell motility and cytoskeletal organization (Actin, Calponin-2, and Keratin). These KBD-associated proteins indicate that cytoskeletal remodeling, glycometabolism, and oxidative stress are abnormal in KBD articular cartilage.
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