期刊
PROTEOMICS
卷 11, 期 24, 页码 4726-4730出版社
WILEY-BLACKWELL
DOI: 10.1002/pmic.201100162
关键词
Cell Lysis; HeLa S3; MS; Protein Digestion; Technology
资金
- German Academic Exchange service
In order to maximize the number of proteins identified from Hela S3 cell lysate we tested various cell lysis, protein precipitation and digestion protocols. First, we compared three different lysis buffers, two mechanical cell disruption methods and two precipitation methods. Then, we tested six different in-solution digestion protocols, three different in-gel digestion protocols and ten different peptide extraction protocols. The result is a proposal for an optimized protocol to prepare the whole cell lysate samples from HeLa S3 cells.
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