Article
Orthopedics
Di Jia, Ruixian Zhang, Yinghong He, Guofeng Cai, Jiali Zheng, Yuye Yang, Yanlin Li
Summary: The Diannan small-ear pig can serve as an effective osteoarthritis model, and inducing osteoarthritis by intra-articular injection of papain is a convenient and fast method worth promoting. The study also explored the role of SDF-1/CXCR4 signaling pathway in the development of osteoarthritis in animals.
JOURNAL OF ORTHOPAEDIC SURGERY AND RESEARCH
(2021)
Article
Biotechnology & Applied Microbiology
Rui Hou, Linhui Gao, Jianhui Liu, Zhen Liang, Yongjin J. Zhou, Lihua Zhang, Yukui Zhang
Summary: This study conducted a label-free quantitative proteomic analysis of the methanol metabolism in methylotrophic yeast and identified the highly up-regulated expression of the transaldolase isoenzyme, which plays a crucial role in improving methanol biotransformation efficiency in Pichia pastoris.
SYNTHETIC AND SYSTEMS BIOTECHNOLOGY
(2022)
Article
Biochemical Research Methods
Rafaela Furioso Ferreira, Thomas Blees, Farhad Shakeri, Andreas Buness, Marc Sylvester, Giovanni Savoini, Alessandro Agazzi, Vladimir Mrljak, Helga Sauerwein
Summary: This study isolated exosomes from porcine milk in different lactation stages and analyzed their content using a TMT-based high-resolution quantitative proteomic approach. Different proteome profiles were revealed for colostrum exosomes and milk exosomes, with functional analysis highlighting pathways related to homeostasis in colostrum exosomes and pathways related to endothelial cell development and lipid metabolism in mature milk exosomes. These findings emphasize the importance of exosomes as active biocomponents of milk and provide insights for future studies on their role in immunity regulation and growth of newborns.
JOURNAL OF PROTEOMICS
(2021)
News Item
Multidisciplinary Sciences
Ewen Callaway
Summary: Advancements in artificial intelligence enable researchers to create original molecules within seconds instead of months.
Article
Agriculture, Dairy & Animal Science
Alessio Di Luca, Andrea Ianni, Francesca Bennato, Camillo Martino, Michael Henry, Paula Meleady, Giuseppe Martino
Summary: This study compared the proteomic differences between Nero d'Abruzzo and commercial-hybrid pigs, revealing significant differences in the protein expression of a large pool of proteins. Certain proteins, such as fatty acid synthase, catalase, and glutathione peroxidase, were found to be differentially expressed and may contribute to the phenotypic differences between the pigs. This work has identified potential proteins that could be useful for new applications in pig breeding programs.
News Item
Multidisciplinary Sciences
Ewen Callaway
Summary: Microbial molecules from soil, seawater, and human bodies are among the least understood substances on Earth.
Review
Biochemistry & Molecular Biology
Anna Gloeckner, Susann Ossmann, Andre Ginther, Jagdip Kang, Michael A. Borger, Alexandro Hoyer, Maja-Theresa Dieterlen
Summary: Porcine models are commonly used for studying the effects of cardioplegic solutions due to their strong anatomical and physiological similarities to humans in cardiovascular aspects. These models offer advantages in simulating conditions seen in human cardiac surgeries, but also come with limitations that need further refinement.
Review
Biochemical Research Methods
Andre Zelanis, Uilla Barcick, Nathalia de Vasconcellos Racorti, Murilo Salardani
Summary: Cellular communication relies on signaling circuits modulated by soluble biomolecules and extracellular vesicles (EVs). Profiling cell line secretomes provides an opportunity to identify signatures of specific cell types with prognostic/therapeutic value. This review focuses on the biological implications of cell secretomes in cancer and their functional roles in shaping the tumoral microenvironment and communication status of participating cells.
Review
Cell Biology
Rachel A. Kline, Lena Loesslein, Dominic Kurian, Judit Aguilar Marti, Samantha L. Eaton, Felipe A. Court, Thomas H. Gillingwater, Thomas M. Wishart
Summary: Recent advances in proteomic technologies have enabled unprecedented assessment of molecular composition in various sample types. However, careful consideration is required when applying these technologies, including methodological selection and analysis workflow. The effectiveness of most proteomics screens is hindered by inadequate analyses, rather than technical limitations. Furthermore, studying progressive neurodegenerative conditions presents inherent difficulties that should be addressed.
Article
Immunology
Hong-Ying Qiu, Na-Na Zhang, Qing-Qing Ma, Rui-Ting Li, Meng-Yue Guan, Li-Li Zhang, Jia Zhou, Rong-Rong Zhang, Xing-Yao Huang, Wen-Hui Yang, Yong-Qiang Deng, Cheng-Feng Qin, Dong-Sheng Zhou
Summary: This study demonstrates that Zika virus can be transmitted via aerosol routes, resulting in systemic infection and inducing immune responses.
EMERGING MICROBES & INFECTIONS
(2022)
News Item
Multidisciplinary Sciences
Ewen Callaway
Summary: AlphaFold neural network has created a 'totally transformative' database with over 350,000 structures from Homo sapiens and 20 model organisms.
Article
Immunology
Pengfei Li, Zhifang Hao, Jingyu Wu, Chen Ma, Yintai Xu, Jun Li, Rongxia Lan, Bojing Zhu, Pengyu Ren, Daidi Fan, Shisheng Sun
Summary: This study systematically investigated the protein expression profiles of M1 and M2 phenotypes in human THP-1 and mouse RAW264.7 macrophages, revealing significant divergences between the two cell lines while also identifying some common up-regulated proteins in M1 macrophages. These data serve as important resources for further studies of macrophage-associated diseases in experimental pathology using human and mouse cell line models.
FRONTIERS IN IMMUNOLOGY
(2021)
Article
Biochemical Research Methods
Stephen R. Piccolo, Paul Denny, Andrew Luxton-Reilly, Samuel H. Payne, Perry G. Ridge
Summary: Computer programming is a valuable tool for life scientists, but learning to code can be difficult for students and researchers in life-sciences disciplines. Recent advancements in artificial intelligence have raised the possibility of using these technologies to assist or even replace the coding efforts of life scientists. In this study, the researchers evaluated the performance of OpenAI's ChatGPT in solving programming exercises from a bioinformatics course, and found that the tool was able to successfully complete a high percentage of the tasks.
PLOS COMPUTATIONAL BIOLOGY
(2023)
Article
Respiratory System
Shahram Parvin, Masoud Arabfard, Ali Ghazvini, Mostafa Ghanei, Ali Najafi
Summary: This study compared the serum proteome of chronic gas-exposed patients and healthy controls, revealing significant differences in protein expression associated with inflammatory and cell adhesion signaling pathways. The findings suggest impaired repair cycles of cell degeneration and regeneration in the injured organs of exposed individuals. The study highlights the role of systems biology in enhancing our understanding of pathophysiological mechanisms and identifying potential disease biomarkers.
BMC PULMONARY MEDICINE
(2022)
Article
Cell Biology
Yao Jiang, Quan Zou, Bo Liu, Shujuan Li, Yi Wang, Tianlong Liu, Xiangdong Ding
Summary: The study found similarities between pig and human follicle morphogenesis, providing a useful model for human hair research and complex hair diseases.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)