Article
Biotechnology & Applied Microbiology
Xiaokang Niu, Anqi Liu, Chunchi Liu, Chong Zhang, Sze Shin Low, Pau Loke Show
Summary: This study prepared chitosan/high methoxyl citrus pectin film catalyzed by Streptomyces coelicolor and found that the film exhibited higher viscosity, better mechanical properties, and improved CO2 barrier performance under a certain catalyst concentration. The internal structure of the film was more uniform, showing promising application in food packaging.
ENVIRONMENTAL TECHNOLOGY & INNOVATION
(2021)
Review
Biochemical Research Methods
Francesco Del Carratore, Erik K. R. Hanko, Rainer Breitling, Eriko Takano
Summary: Streptomyces is a significant genus in biotechnology that produces numerous bioactive compounds, including clinically relevant drugs. Recent advances in genetic engineering and molecular biology have greatly enhanced the ability of Streptomyces to function as cell factories and expand their biotechnological potential.
CURRENT OPINION IN BIOTECHNOLOGY
(2022)
Article
Environmental Sciences
Xiaomei Liu, Jingkang Ma, Saisai Guo, Qingying Shi, Jingchun Tang
Summary: The combined toxicity of nanoplastics and DBP on bacteria was studied, and it was found that the toxicity of nanoplastics decreased when combined with higher concentrations of DBP. The combination of 10 mg/L nanoplastics and 5 mg/L DBP showed the highest impact on bacteria.
SCIENCE OF THE TOTAL ENVIRONMENT
(2022)
Article
Biotechnology & Applied Microbiology
Maral Tsevelkhoroloo, Vijayalakshmi Dhakshnamoorthy, Young-Soo Hong, Chang-Ro Lee, Soon-Kwang Hong
Summary: In this study, two α-neoagarooligosaccharide hydrolases (SCO3481 and SCO3479) were identified in Streptomyces coelicolor A3(2), which catalyzed the hydrolysis of α-(1,3) glycosidic bonds. SCO3481 belonged to the GH117 family and formed dimers, while SCO3479 was a large monomeric α-neoagarooligosaccharide hydrolase belonging to the GH2 family with β-galactosidase activity. SCO3479, a unique bifunctional enzyme, has many advantages for industrial applications.
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
(2023)
Article
Environmental Sciences
Xiaomei Liu, Jingkang Ma, Chengliang Yang, Lan Wang, Jingchun Tang
Summary: This study found that the toxicity and mechanism of nano/microplastics on Streptomyces coelicolor varied significantly with different concentrations, affecting cell membrane permeability, ROS levels, and antibiotic production. The effects were size and concentration dependent.
SCIENCE OF THE TOTAL ENVIRONMENT
(2021)
Article
Microbiology
Sanne Westhoff, Alexander M. Kloosterman, Stephan F. A. van Hoesel, Gilles P. van Wezel, Daniel E. Rozen
Summary: Bacteria compete for resources and space by producing antibiotics that inhibit competitors, with antibiotic production influenced by social cues and resource limitation. Antibiotic production is more likely to be induced by cues from closely related strains or those sharing biosynthetic gene clusters, but not by competitors that inhibit growth. Cells often reduce antibiotic production in the presence of competitors, especially under nutrient limitation, highlighting the regulation of antibiotic production by interactions between social and resource environments.
Article
Multidisciplinary Sciences
Toshiki Nagakubo, Tatsuya Yamamoto, Shumpei Asamizu, Masanori Toyofuku, Nobuhiko Nomura, Hiroyasu Onaka
Summary: Studies have found that Streptomyces can affect microbial competition by producing Streptomyces phage tail-like particles (SLPs), with SLP expression changing according to the competitor species, showing different responses to fungi and Bacillus subtilis.
SCIENTIFIC REPORTS
(2021)
Article
Microbiology
Sarayu Bhogoju, Collins N. Khwatenge, Thyneice Taylor-Bowden, Gabriel Akerele, Boniface M. Kimathi, Joseph Donkor, Samuel N. Nahashon
Summary: The study suggests that Lactobacillus reuteri and Streptomyces coelicolor either separately or in combination have the potential to serve as probiotics in chickens, leading to improvements in body weight gain, feed consumption, and feed conversion ratios.
Article
Ecology
Alise R. Muok, Dennis Claessen, Ariane Briegel
Summary: Streptomyces spores can utilize the motility machinery of other soil bacteria to be transported to plant tissues by interacting with the flagella of both gram-positive and gram-negative bacteria. This highlights the capability of nonmotile bacteria to use the motility machinery of other microbes to complete necessary stages of their lifecycle.
Review
Biochemical Research Methods
Hahk-Soo Kang, Eung-Soo Kim
Summary: Heterologous expression is a key platform for linking natural product chemotypes to their genotypes. Advances in sequencing and bioinformatics have enabled rapid identification of known and cryptic BGCs from microbial genomes. Synthetic biology tools have facilitated the cloning and engineering of BGCs to enhance production yield, activating silent BGCs. Heterologous expression in Streptomyces hosts will continue to play a vital role in genomics-driven natural product research.
CURRENT OPINION IN BIOTECHNOLOGY
(2021)
Article
Engineering, Environmental
Xiaomei Liu, Shakeel Ahmad, Jingkang Ma, Dan Wang, Jingchun Tang
Summary: This study investigated the short-term toxicity of nanoplastics released from biodegradable and conventional plastics on Streptomyces coelicolor. The study found that biodegradable plastics released more nanoplastics compared to conventional plastics, and these nanoplastics showed significant toxicity to the bacteria. Additionally, the addition of biodegradable plastics significantly increased the production of antibiotics.
JOURNAL OF HAZARDOUS MATERIALS
(2023)
Article
Biochemical Research Methods
Rongbin Wang, Jennifer Nguyen, Jacob Hecht, Nora Schwartz, Katelyn Brown, Larissa Ponomareva, Magdalena Niemczura, Dino van Dissel, Gilles P. van Wezel, Jon S. Thorson, Mikko Metsa-Ketela, Khaled A. Shaaban, S. Eric Nybo
Summary: By developing the BIOPOLYMER toolbox, we have overcome the limitations of slow growth and lack of genetic tools in actinomycetes for the synthesis of anthracyclinones. We successfully improved the production host and optimized the production of anthracyclinones, identifying optimal combinations for different molecules. Additionally, we demonstrated the moderate cytotoxicity of the anthracyclinones against multiple cancer cell lines.
ACS SYNTHETIC BIOLOGY
(2022)
Article
Biotechnology & Applied Microbiology
Xiaojing Wang, Jing Liu, Hanzhi Zhang, Kai Wu, Zhijun Yang, Ruonan Ning, Junhai Huang, Lei Shao
Summary: The overexpression of the deacylase gene in different Streptomyces strains, particularly in recombinant S. coelicolor, significantly increased the efficiency of daptomycin nucleus production. Optimized bioconversion conditions demonstrated an increase in conversion efficiency from 71.04% to 85.02%, showing potential for industrial applications. The study provides valuable insights for enhancing daptomycin nucleus production and potential commercialization of overproducing strains for antibacterial drug development.
JOURNAL OF CHEMICAL TECHNOLOGY AND BIOTECHNOLOGY
(2021)
Article
Biochemistry & Molecular Biology
D. Somasekhara, Manjunath Dammalli, Varalakshmi Kilingar Nadumane
Summary: This study assessed the anticancer potential of a yellow pigment fraction, OR3, isolated from Streptomyces coelicolor JUACT03, on breast cancer cells. It found that OR3 treatment decreased cell proliferation and induced apoptotic cell death through dysregulation of protein expressions and signaling pathways involved in cell proliferation and apoptosis.
APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY
(2023)
Review
Biotechnology & Applied Microbiology
Shangping Jin, Ming Hui, Yinhua Lu, Yawei Zhao
Summary: The two-component system (TCS) is a regulatory system that regulates cellular behavior by responding to stimuli. It is widely present in prokaryotes and plays a crucial role in bacterial signaling. TCSs have been studied in the industrially important microbe Streptomyces, which produces bioactive secondary metabolites (SMs). The genetic engineering of TCSs can enhance SMs production, making it valuable for industrial-scale manufacturing. This review summarizes recent advances in understanding the functional identification and regulatory mechanisms of TCSs in Streptomyces coelicolor, with a focus on their involvement in SMs biosynthesis.
WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY
(2023)
Article
Endocrinology & Metabolism
Weigang Cai, Michaela Egertova, Cleidiane G. Zampronio, Alexandra M. Jones, Maurice R. Elphick
Summary: This study provides the first insights into the expression and function of CRH-type neuropeptides in the unique context of the pentaradially symmetrical body plan of an echinoderm. The neuropeptide ArCRH was found to have a widespread pattern of expression in the central nervous system and locomotory organs of the starfish Asterias rubens.
NEUROENDOCRINOLOGY
(2023)
Article
Microbiology
Rebecca C. Lowry, Laila Allihaybi, Jennifer L. Parker, Narciso A. S. Couto, Graham P. Stafford, Jonathan G. Shaw
Summary: Bacterial swimming is mediated by the rotation of a flagellar filament. Many bacteria can O-glycosylate their flagellins, but the specific role of glycosylation is still unclear. This study investigated the localization of glycans on the flagellum filament of Aeromonas caviae and found that pseudaminic acid O-glycosylation was heterogeneous with no constantly glycosylated sites. Mutations in glycosylation sites resulted in reduced motility and flagellin production. Site-directed mutagenesis of hydrophobic amino acids flanking the glycosylation sites also led to decreased motility and flagellin levels, indicating their role in flagellin glycosylation.
Article
Microbiology
Ian D. E. A. Lidbury, Sebastien Raguideau, Chiara Borsetto, Andrew R. J. Murphy, Andrew Bottrill, Senlin Liu, Richard Stark, Tandra Fraser, Andrew Goodall, Alex Jones, Gary D. Bending, Mark Tibbet, John P. Hammond, Chris Quince, David J. Scanlan, Jagroop Pandhal, Elizabeth M. H. Wellington
Summary: Advances in DNA sequencing technologies have expanded our understanding of the plant microbiome, but our ability to identify the metabolically active fraction of soil microbiota and its role in plant health is still limited. In this study, a unique protein extraction method and bioinformatics pipeline were used to identify extracellular proteins synthesized in the rhizosphere of Brassica spp. The study revealed significant changes in the metabolically active fraction of the soil microbiota in response to the presence of B. napus roots, including stimulation of rhizosphere-specialized bacteria and upregulation of plant beneficial functions.
Article
Biology
Nayeli Escudero Castelan, Dean C. Semmens, Luis Alfonso Yanez Guerra, Meet Zandawala, Mario Dos Reis, Susan E. Slade, James H. Scrivens, Cleidiane G. Zampronio, Alexandra M. Jones, Olivier Mirabeau, Maurice R. Elphick
Summary: This study identified an expanded family of eleven kisspeptin-type receptors in a deuterostome invertebrate, the starfish Asterias rubens, along with neuropeptides as ligands for these receptors, shedding light on the diversity and evolution of kisspeptin signaling in starfish. The discovery of the relationship between kisspeptins and SALMFamides in starfish presents a novel perspective for research on kisspeptin signaling.
Article
Medicine, Research & Experimental
Alison Hammond, Kathryn A. Radford, Angela Ching, Yeliz Prior, Rachel O'Brien, Sarah Woodbridge, June Culley, Jennifer Parker, Paula Holland
Summary: This study is a multi-center randomized controlled trial that aims to evaluate the effectiveness and cost-effectiveness of job retention vocational rehabilitation for employed people with inflammatory arthritis. The study will conduct a process evaluation to assess implementation fidelity and explore factors related to future implementation in clinical practice.
Article
Medicine, Research & Experimental
Angela Ching, Jennifer Parker, Alexandra Haig, Chris J. Sutton, Sarah Cotterill, Denise Forshaw, June Culley, Alison Hammond
Summary: People with inflammatory arthritis often experience high levels of work disability, absenteeism, and presenteeism. WORKWELL is a trial that aims to test the effectiveness and cost-effectiveness of a job retention vocational rehabilitation program for employed individuals with this condition. Due to the COVID-19 pandemic, the trial had to pause recruitment and intervention delivery, but managed to adapt its protocol and procedures to successfully restart within a short period of time. The trial implemented new strategies for recruitment, consent, and intervention delivery, and achieved recruitment figures above the pre-COVID target with a high retention rate.
Article
Orthopedics
Angela Ching, Yeliz Prior, Jennifer Parker, Alison Hammond
Summary: This systematic review aims to identify the impact of osteoarthritis (OA) on work participation and biopsychosocial and work-related factors associated with absenteeism, presenteeism, work transitions, work impairment, work accommodations, and premature work loss. The study found that physically intensive work, moderate-to-severe joint pain, comorbidities, and low co-worker support potentially affect work participation in OA.
BMC MUSCULOSKELETAL DISORDERS
(2023)
Article
Multidisciplinary Sciences
Arsheed H. Sheikh, Iosif Zacharia, Alonso J. Pardal, Ana Dominguez-Ferreras, Daniela J. Sueldo, Jung-Gun Kim, Alexi Balmuth, Jose R. Gutierrez, Brendon F. Conlan, Najeeb Ullah, Olivia M. Nippe, Anil M. Girija, Chih-Hang Wu, Guido Sessa, Alexandra M. E. Jones, Murray R. Grant, Miriam L. Gifford, Mary Beth Mudgett, John P. Rathjen, Vardis Ntoukakis
Summary: In both plants and animals, nucleotide-binding leucine-rich repeat (NLR) immune receptors play critical roles in pathogen recognition and activation of innate immunity. By studying the tomato Prf/Pto NLR resistance complex, the researchers identified the 14-3-3 proteins TFT1 and TFT3 as interacting partners of the NLR complex and the protein kinase MAPKKK alpha. They also found that the helper NRC proteins are integral components of the Prf/Pto NLR recognition complex. The study provides mechanistic insights into the activation of immune receptors and initiation of downstream signaling cascades.
NATURE COMMUNICATIONS
(2023)
Article
Rheumatology
Alison Hammond, Alan Tennant, Angela Ching, Jennifer Parker, Yeliz Prior, Monique A. M. Gignac, Suzanne M. M. Verstappen, Rachel O'Brien
Summary: The study aimed to validate linguistically British-English versions of the LTCJSS, LTCWSS, and WHPLPS in rheumatoid arthritis, axial spondyloarthritis, osteoarthritis, and fibromyalgia (FM). The scales were assessed for construct and concurrent validity using Rasch analysis, and test-retest reliability was also measured.
MUSCULOSKELETAL CARE
(2023)
Article
Rheumatology
Tamara Brown, Alison Hammond, Angela Ching, Jennifer Parker
Summary: This study aims to examine the work limitations of individuals with rheumatic and musculoskeletal diseases (RMD) and identify factors associated with presenteeism. The results show that these diseases have a significant impact on work participation, and certain factors such as functional limitations and pain are associated with higher work limitations. The study highlights the importance of work support, rehabilitation, and healthy workplace practices.
MUSCULOSKELETAL CARE
(2023)
Article
Rheumatology
Alison Hammond, Alan Tennant, Angela Ching, Jennifer Parker, Yeliz Prior, Monique A. M. Gignac, Suzanne M. M. Verstappen, Rachel O'Brien
Summary: This study aimed to validate British-English versions of the Perceived Workplace Support Scale (PWSS), Work Accommodations, Benefits, Policies and Practices Scale (WABPPS), and Work Transitions Index (WTI) in patients with rheumatoid arthritis (RA), axial spondyloarthritis (axSpA), osteoarthritis (OA), and fibromyalgia (FM). The scales were adapted into British-English and reviewed by experts before cognitive debriefing interviews. The findings showed that the British-English versions of PWSS, WABPPS, and WTI are reliable and valid for use in research, organizational level studies, and vocational rehabilitation.
MUSCULOSKELETAL CARE
(2023)
Article
Rheumatology
Alison Hammond, Rachel O'Brien, Sarah Woodbridge, Jennifer Parker, Angela Ching
Summary: This study revised the Work Experience Survey-Rheumatic Conditions (WES-RC-UK), a questionnaire listing workplace barriers, and investigated its content validity, reliability, and concurrent validity. The results showed that the WES-RC has good content validity and reliability, emphasizing the need for biopsychosocial work rehabilitation.
MUSCULOSKELETAL CARE
(2023)
Article
Rheumatology
Alison Hammond, Alan Tennant, Tamara Brown, Yeliz Prior, Angela Ching, Jennifer Parker
Summary: The study aimed to determine the applicability of the current form of the RA-WIS scale for employed individuals with fibromyalgia to assess the risk of work disability and the need for work rehabilitation. The results showed that the RA-WIS scale has good validity and reliability in a group setting for individuals with fibromyalgia, but further development is needed for individual use.
MUSCULOSKELETAL CARE
(2023)
Article
Rheumatology
Alison Hammond, Alan Tennant, Angela Ching, Jennifer Parker, Yeliz Prior, Monique A. M. Gignac, Suzanne M. M. Verstappen, Rachel O'Brien
Summary: This study translated and validated the Workplace Activity Limitations Scale (WALS) into British English and tested its psychometric properties in different arthritis conditions. The results showed good validity, reliability, and concurrent validity of the WALS, indicating that it can be used as an effective tool to assess work difficulties.
RHEUMATOLOGY ADVANCES IN PRACTICE
(2022)
Article
Biochemistry & Molecular Biology
Benjamin Petre, Mauricio P. Contreras, Tolga O. Bozkurt, Martin H. Schattat, Jan Sklenar, Sebastian Schornack, Ahmed Abd-El-Haliem, Roger Castells-Graells, Rosa Lozano-Duran, Yasin F. Dagdas, Frank L. H. Menke, Alexandra M. E. Jones, Jack H. Vossen, Silke Robatzek, Sophien Kamoun, Joe Win
Summary: By transiently expressing RXLR effectors of the potato blight pathogen Phytophthora infestans in Nicotiana benthamiana leaves, an effector-host protein interactome matrix was generated, revealing over 35 biological processes potentially targeted by P. infestans. This study provides a valuable resource for functional studies of P. infestans effectors and effector-targeted host processes.
