Review
Geriatrics & Gerontology
Hai-ling Wang, Chun-lin Zhang, Yan-mei Qiu, An-qi Chen, Ya-nan Li, Bo Hu
Summary: Cerebral microbleeds (CMBs) are small lesions in the brain's microvessels that are linked to various neurological conditions. The exact pathogenesis and treatment options for CMBs require further investigation and research.
Article
Neurosciences
Yanzhao Zhou, Feng Yan, Xue Han, Xin Huang, Xiang Cheng, Yanan Geng, Xiufang Jiang, Ying Han, Ming Zhao, Lingling Zhu
Summary: NB-3 plays an important role in neural development and injury recovery. It maintains the integrity of the blood brain barrier under high-altitude cerebral edema conditions. Deficiency of NB-3 leads to BBB leakage and downregulation of tight junction proteins, while overexpression or supplementation with NB-3 can alleviate endothelial barrier injuries.
EXPERIMENTAL NEUROLOGY
(2022)
Review
Biochemistry & Molecular Biology
Ruicheng Yang, Jundan Wang, Fen Wang, Huipeng Zhang, Chen Tan, Huanchun Chen, Xiangru Wang
Summary: Despite advances in treatment, bacterial meningitis remains a serious infection that poses a risk to life. The blood-brain barrier plays an important role in the pathogenesis of bacterial meningitis through increased permeability. Recent studies have shown that targeting the factors contributing to enhanced blood-brain barrier permeability is an effective complement to antimicrobial therapy.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Clinical Neurology
Muhammad Ali, Kaja Falkenhain, Brendah N. Njiru, Muhammad Murtaza-Ali, Nancy E. Ruiz-Uribe, Mohammad Haft-Javaherian, Stall Catchers, Nozomi Nishimura, Chris B. Schaffer, Oliver Bracko
Summary: VEGF-A signalling contributes to blood-brain barrier permeability and capillary stalls in a mouse model of Alzheimer's disease. Inhibiting VEGF-A signalling can reduce capillary stalls and increase cerebral blood flow.
Review
Clinical Neurology
Jonathan Siegel, Shahil H. Patel, Berk Mankaliye, Ami P. Raval
Summary: Electronic cigarettes (ECs) are gaining global popularity as battery-powered nicotine delivery devices. Although EC aerosols contain fewer harmful toxins than conventional cigarettes (CCs), research has shown that they still contain harmful ingredients. Nicotine, a common ingredient in both ECs and CCs, is highly addictive and toxic. The long-term effects of EC use on the brain are still not well understood, and this review aims to explore the potential impact of ECs on stroke risk and severity.
TRANSLATIONAL STROKE RESEARCH
(2022)
Article
Cell Biology
Xu Wendeng, Gao Ceshu, Wu Jian
Summary: CD151 overexpression protects BBB permeability in the early phase after transient focal cerebral ischemia, demonstrating its potential as a target for early BBB protection in ischemic stroke.
CELLULAR AND MOLECULAR NEUROBIOLOGY
(2021)
Review
Biochemistry & Molecular Biology
Nurul Sulimai, Jason Brown, David Lominadze
Summary: Neurons and glial cells in the brain are protected by the blood brain barrier (BBB). Local regulation of blood flow in the brain is determined by neurons and astrocytes. Changes in cerebral vessels, particularly in cerebrovascular permeability, play a crucial role in the development and progression of neuroinflammatory and neurodegenerative diseases, resulting in memory decline.
Article
Biochemistry & Molecular Biology
Manjusha Thakar, Midrelle E. Noumbissi, Monique F. Stins
Summary: Postmortem neuropathological studies have revealed regional differences in the brain diseases, such as cerebral malaria, which shows more hemorrhagic punctae in the white matter than grey matter. The underlying cause of these differences is unclear. In this study, researchers investigated the impact of the vascular microenvironment on the expression of endothelial protein C receptor (EPCR) and found that EPCR expression in cerebral microvessels is heterogeneous in the white matter compared to the grey matter. Their findings provide insights into the origin of molecular phenotypic heterogeneity at the microvascular level and may contribute to a better understanding of variations in neuropathology associated with vasculature in different brain regions.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Oncology
Jinghuan Wang, Wen Zhong, Qianwen Cheng, Chenxi Xiao, Jie Xu, Zhenghua Su, Haibi Su, Xinhua Liu
Summary: Smyd2 plays a vital role in disrupting blood-brain barrier integrity in ischaemic stroke through methylation-mediated Sphk/S1PR, and knocking down Smyd2 reduces blood-brain barrier permeability and improves functional recovery.
CLINICAL AND TRANSLATIONAL MEDICINE
(2022)
Review
Parasitology
Maria Bernabeu, Caitlin Howard, Ying Zheng, Joseph D. Smith
Summary: The pathogenesis of Plasmodium falciparum is complex and closely related to vascular physiology, especially evident in cerebral malaria. Recent bioengineering approaches in generating microvessels have provided new insights into malaria research, offering great potential for understanding the mechanisms of the disease in greater depth.
TRENDS IN PARASITOLOGY
(2021)
Review
Pharmacology & Pharmacy
Jui-Ming Sun, Ting-Lin Yen, Jing-Shiun Jan, Pharaoh Fellow Mwale, Ruei-Dun Teng, Rajeev Taliyan, Cheng-Ta Hsieh, Chih-Hao Yang
Summary: Cerebral ischemia is a sudden reduction in blood flow to the brain, depriving it of glucose and oxygen. Stroke is the main type of cerebral ischemia, and current treatments have limitations. Monoclonal antibody therapy has shown effectiveness in neurological disorders, but its use in stroke treatment is limited. Enhancing blood-brain barrier penetration is crucial for improving the efficacy of antibody-based therapeutics in cerebral ischemia.
Article
Hematology
Felecia M. Marottoli, Hui Zhang, Eden Flores-Barrera, Emilce Artur de la Villarmois, Frederick C. Damen, Anabel M. M. Miguelez Fernandez, Hannah V. Blesson, Rohan Chaudhary, Anthony L. Nguyen, Amanda E. Nwokeji, Ruju Talati, Ashwin S. John, Kushi Madadakere, Sarah E. Lutz, Kejia Cai, Kuei Y. Tseng, Leon M. Tai
Summary: The loss of endothelial cell APOE3 expression leads to neurovascular dysfunction and abnormalities in neural function. This finding supports the importance of endothelial cell APOE3 in neurovascular regulation, neural circuit function, and behavior.
ARTERIOSCLEROSIS THROMBOSIS AND VASCULAR BIOLOGY
(2023)
Article
Medicine, Research & Experimental
Zhi Fang, Xiaoran Sun, Xiang Wang, Ji Ma, Thomas Palaia, Ujala Rana, Benjamin Miao, Louis Ragolia, Wenquan Hu, Qing Robert Miao
Summary: The loss of cerebral cavernous malformation (CCM) genes leads to vascular lesions in the brain. This study reveals that genetic depletion of the NOGOB receptor (NGBR) in endothelial cells (ECs) results in CCM1/2 expression deficiency and cerebrovascular lesions. Mechanistically, NGBR is required for maintaining the expression of CCM1/2 in ECs through HBO1-mediated histone acetylation.
JOURNAL OF CLINICAL INVESTIGATION
(2022)
Article
Biochemistry & Molecular Biology
Qiuling Liu, Tujing Song, Bing Chen, Jingjing Zhang, Wen Li
Summary: Hypoxia is crucial for the development of various disorders, especially hypoxic cerebropathy. The damage to the blood-brain barrier (BBB) caused by hypoxia is well known. This study explored the correlation between endothelial ferroptosis and hypoxia-induced BBB damage using in vivo zebrafish and in vitro bEnd.3 cells. The results showed that hypoxic treatment could induce BBB disruption by down-regulating claudin-5 (CLDN5) expression in both zebrafish cerebrovascular endothelial cells and bEnd.3 cells.
Article
Pharmacology & Pharmacy
Jared R. Wahl, Anjali Vivek, Seph M. Palomino, Moyad Almuslim, Karissa E. Cottier, Paul R. Langlais, John M. Streicher, Todd W. Vanderah, Erika Liktor-Busa, Tally M. Largent-Milnes
Summary: This study investigates the role of cortical spreading depression (CSD) in blood-brain barrier (BBB) pathology using an in vitro model. The findings suggest that CSD can lead to relocalization and functional alteration of proteins associated with the actin cytoskeleton and endothelial tight junctions. Additionally, unique phosphorylation signatures were identified in phospho-proteomic analysis, highlighting Zona Occludins 1 (ZO-1) as a potential pathologic checkpoint of the BBB.
Article
Biochemistry & Molecular Biology
Sandy Theysgeur, Benoit Cudennec, Barbara Deracinois, Claire Perrin, Isabelle Guiller, Anne Lepoudere, Christophe Flahaut, Rozenn Ravallec
Summary: Research shows that fish protein hydrolysates have promising effects on regulating food intake and glucose metabolism in dogs, as well as influencing intestinal hormone secretion and DPP-IV inhibitory activity. New bioactive peptides have been identified to stimulate CCK and GLP-1 secretions and inhibit DPP-IV activity.
Article
Fisheries
Julia Mougin, Roxane Roquigny, Christophe Flahaut, Maryse Bonnin-Jusserand, Thierry Grard, Cedric Le Bris
Summary: The study highlights the threat of vibriosis in seabass aquaculture, mainly caused by Vibrio harveyi. Monitoring the presence of V. harveyi in water and biofilms over time is crucial for understanding the dynamics of the pathogen. The constant presence of V. harveyi in rearing tanks and water inlets suggests an increased risk of vibriosis outbreaks, especially with rising water temperatures.
Article
Food Science & Technology
Dahlia Daher, Barbara Deracinois, Philippe Courcoux, Alain Baniel, Sylvie Chollet, Renato Froidevaux, Christophe Flahaut
Summary: This study investigated the enzymatic hydrolysis kinetics of micellar milk caseins for 9 hours, finding that 5 hours is the optimal time to obtain hydrolysates with desirable sensory qualities. A total of 22 peptides formed during the enzymatic proteolysis of micellar caseins were identified as having the greatest influence on bitterness, providing simple and efficient tools to predict the sensory characteristics of micellar casein enzymatic hydrolysates.
Review
Food Science & Technology
Mathie Tenenbaum, Barbara Deracinois, Camille Dugardin, Aurelie Mateos, Audrey Romelard, Julie Auger, Audrey Boulier, Rozenn Ravallec, Christophe Flahaut, Benoit Cudennec
Summary: Milk and dairy products are important sources of proteins and peptides that have significant impact on human health. CPPs, bioactive phosphorylated peptides resulting from the hydrolysis of caseins, have attracted increasing interest due to their ability to chelate minerals and increase their bioavailability, as well as their antioxidant and anti-inflammatory properties. This review discusses the analytical characterization, release during gastrointestinal digestion, obtention processes, physicochemical characteristics, and bioactive roles of CPPs. It highlights the potential of CPPs in treating various pathologies and the need for further research to elucidate their mechanisms of action and potential for human health.
FOOD RESEARCH INTERNATIONAL
(2022)
Article
Microbiology
Elodie Dussert, Melissa Tourret, Chloe Dupuis, Alexandre Noblecourt, Josette Behra-Miellet, Christophe Flahaut, Rozenn Ravallec, Francois Coutte
Summary: This study investigated the antiradical and antioxidant potential of lipopeptides produced by Bacillus subtilis strains. The results showed that plipastatin exhibited the highest antioxidant activities.
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Pharmacology & Pharmacy
Desire Madi-Moussa, Barbara Deracinois, Radja Teiar, Yanyan Li, Marius Mihasan, Christophe Flahaut, Sylvie Rebuffat, Francoise Coucheney, Djamel Drider
Summary: This study reveals that the bacteriocin lacticaseicin 30, a rare Gram-positive bacteriocin that inhibits Gram-negative bacteria, requires at least two helices in the N-terminal region and carries amino acids in the C-terminal region that play a role in modulating its activity. These findings are crucial for designing lacticaseicin 30 variants as potential therapeutic agents against Gram-negative bacilli infections.
Article
Biochemistry & Molecular Biology
Anne-Sophie Paguet, Ali Siah, Gabriel Lefevre, Sophie Moureu, Thierry Cadalen, Jennifer Samaillie, Franck Michels, Barbara Deracinois, Christophe Flahaut, Harmony Alves Dos Santos, Audrey Etienne-Debaecker, Caroline Rambaud, Sylvie Chollet, Roland Molinie, Jean-Xavier Fontaine, Christophe Waterlot, Marie-Laure Fauconnier, Sevser Sahpaz, Celine Riviere
Summary: This study investigates the genetic and chemical diversity of fifty wild hops collected from different locations in Northern France. The results show that wild hops exhibit high levels of genetic and chemical diversity, making them an important resource for the species.
Article
Food Science & Technology
Fatma Hamed, Imen Elgaoud, Barbara Deracinois, Christophe Flahaut, Naima Nedjar, Ahmed Barkia
Summary: In this study, Diplodus proteins were hydrolyzed using alcalase and savinase, and the hydrolysates were characterized for their chemical composition, functional properties and peptide content. The hydrolysates produced with savinase had a higher protein content and were richer in hydrophobic peptides compared to those produced with alcalase. Savinase hydrolysate also showed better solubility, foaming properties, and oil and water retention capacities, while alcalase hydrolysate had better emulsifying property and emulsion stability. These results suggest that alcalase and savinase can be used to convert Diplodus annularis proteins into peptides with promising functional properties.
Article
Cell Biology
Marie-Pierre Dehouck, Masanori Tachikawa, Yutaro Hoshi, Kotaro Omori, Claude-Alain Maurage, Guillaume Strecker, Lucie Dehouck, Marie-Christine Boucau, Yasuo Uchida, Fabien Gosselet, Tetsuya Terasaki, Yannis Karamanos
Summary: This study compared the protein expression levels of human brain-like endothelial cells (hBLECs) and human brain microvessels (hBMVs) using quantitative targeted absolute proteomics. The results showed that hBLECs had higher protein levels of CD144, CD147, MRP4, Annexin A6, and caveolin-1 compared to hBMVs, while the protein levels of MCT1, Connexin 43, TfR1, and claudin-5 were relatively lower. Furthermore, BCRP was found to be more expressed than MDR1 in hBLECs. These findings contribute to a better understanding of the characteristics of hBLECs and demonstrate the equivalence of in vitro BBB models with in vivo models in terms of transport properties.
Article
Cell Biology
Camille Menaceur, Johan Hachani, Shiraz Dib, Sophie Duban-Deweer, Yannis Karamanos, Fumitaka Shimizu, Takashi Kanda, Fabien Gosselet, Laurence Fenart, Julien Saint-Pol
Summary: This study investigates the proteome differences between brain pericytes (BPs) cultured alone (hBP-solo) and in co-culture with brain microvessel endothelial cells (ECs) (hBP-coc) to model the human blood-brain barrier (BBB) in vitro. The results show that hBP-solo has 51 proteins related to cell proliferation, contractility, adhesion, and extracellular matrix elements, indicating an immature cell state. On the other hand, hBP-coc has 90 proteins associated with reduced contractile activities and enhanced metabolic functions, particularly in mitochondrial activities and sterol metabolism, suggesting mature pericytes. This study highlights the role of ECs in promoting the maturation and metabolic switch of BPs during barriergenesis.
Article
Food Science & Technology
Nathalie Atallah, Claire Gaudichon, Audrey Boulier, Alain Baniel, Benoit Cudennec, Barbara Deracinois, Rozenn Ravallec, Christophe Flahaut, Dalila Azzout-Marniche, Nadezda Khodorova, Martin Chapelais, Juliane Calvez
Summary: The aim of this study was to analyze the protein digestibility and postprandial metabolism in rats of milk protein matrices obtained by different industrial processes. The results showed that ingestion of whey proteins led to higher protein content in the intestinal mucosa and liver, possibly due to fast assimilation. Additionally, industrial processes that modify the structure and/or composition of milk proteins influence protein digestion and utilization.
FOOD RESEARCH INTERNATIONAL
(2023)
Article
Biochemistry & Molecular Biology
Ahlam Outman, Barbara Deracinois, Christophe Flahaut, Mira Abou Diab, Bernard Gressier, Bruno Eto, Naima Nedjar
Summary: Human hemoglobin and bovine hemoglobin both contain rich bioactive peptides, with high similarity between the two. Bioinformatics tools and enzymatic hydrolysis experiments revealed the presence of various bioactive peptides, including some that have never been found in human hemoglobin before.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biochemistry & Molecular Biology
Ahlam Outman, Barbara Deracinois, Christophe Flahaut, Mira Abou Diab, Jihen Dhaouefi, Bernard Gressier, Bruno Eto, Naima Nedjar
Summary: This study focuses on the enzymatic hydrolysis of hemoglobin and compares the antibacterial and antioxidant potentials of human and bovine hemoglobin hydrolysates. The results show strong antimicrobial and antioxidant activities of the peptide hydrolysates, independent of the initial substrate concentration level. The presence of bioactive peptides in human hemoglobin suggests its potential as a valuable source for the food or pharmaceutical industries.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Food Science & Technology
Mouna Ambli, Barbara Deracinois, Anne-Sophie Jenequin, Rozenn Ravallec, Benoit Cudennec, Christophe Flahaut
Summary: Bioinformatics software is essential in peptidomics/proteomics research, enabling the identification of peptides through the comparison of peptide fragmentation spectra obtained by mass spectrometry with targeted databases or by de novo sequencing. Customized software programming is crucial in improving peptide and post-translational modification (PTM) identification rates in the agrofood field.
Article
Biochemistry & Molecular Biology
Lea Fleury, Barbara Deracinois, Camille Dugardin, Alice B. Nongonierma, Richard J. FitzGerald, Christophe Flahaut, Benoit Cudennec, Rozenn Ravallec
Summary: This study compared the DPP-IV inhibitory potential of seven proteins from different sources in vitro and in rat plasma, revealing the impact of protein origin on their in vivo and in vitro DPP-IV inhibitory activity. The results indicated that vegetable proteins and hemoglobin exhibited the highest DPP-IV inhibitory activity.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
