Article
Pathology
Ana Pavlic, Kristian Urh, Emanuela Bostjancic, Nina Zidar
Summary: The aim of this study was to compare the advantages and limitations of the punching tissue block (PTB) and laser capture microdissection (LCM) sampling techniques when studying gene expression patterns in the invasive front of colorectal cancer (CRC). The results showed that PTB is suitable for studying differences between the central part and the invasive front of CRC, whereas LCM is essential for analyzing and comparing morphologically distinct patterns along the invasive front.
PATHOLOGY RESEARCH AND PRACTICE
(2023)
Article
Biochemistry & Molecular Biology
Chiara M. A. Cefalo, Teresa Mezza, Andrea Giaccari, Rohit N. Kulkarni
Summary: This study compared four methods for RNA extraction and found that the RNeasy MicroKit + Carrier (Qiagen) method provided the highest yield and purity.
Review
Biochemistry & Molecular Biology
Bhavana Hemantha Rao, Pavel Soucek, Viktor Hlavac
Summary: The heterogeneity of pancreatic cancer complicates cancer research, and traditional tissue analysis methods are no longer sufficient. The application of single-cell separation techniques helps researchers better understand specific cell populations in pancreatic cancer.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Cell Biology
Xiaodan Zhang, Chuansheng Hu, Chen Huang, Ying Wei, Xiaowei Li, Miaomiao Hu, Hua Li, Ji Wu, Daniel M. Czajkowsky, Yan Guo, Zhifeng Shao
Summary: This method presents a robust approach for acquiring finely resolved transcriptional programs from clinical tissues using immunofluorescence-guided laser capture microdissection. It allows analysis of transcriptional networks and signaling pathways of specific cell types within native tissues. The use of a small molecule inhibitor, RVC, protects the RNA from degradation during the procedure.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2022)
Article
Chemistry, Multidisciplinary
Xiaobo Li, Qi Qi, Yong Li, Qun Miao, Wenqian Yin, Jinghua Pan, Zhan Zhao, Xiaoying Chen, Fan Yang, Xiaofeng Zhou, Maohua Huang, Chenran Wang, Lijuan Deng, Dandan Huang, Ming Qi, Shuran Fan, Yiran Zhang, Shenghui Qiu, Weiqing Deng, Tongzheng Liu, Minfeng Chen, Wencai Ye, Dongmei Zhang
Summary: This study identified the overexpression of TRP channel-associated factor 2 (TCAF2) in tumor initiating cells (TPCs) from colorectal cancer liver metastasis (CRCLM) patients, and demonstrated its role in promoting tumor cell motility, epithelial-mesenchymal transition (EMT), and CRCLM. Mechanistically, TCAF2 inhibits the expression and activity of TRPM8, leading to Wnt5a secretion in TPCs, which facilitates EMT via the activation of the STAT3 signaling pathway. This study provides a potential diagnostic biomarker and therapeutic target for CRCLM.
Article
Immunology
Xinyu Yang, Yinggai Song, TianYu Liang, Qiqi Wang, Ruoyu Li, Wei Liu
Summary: Coccidioidomycosis is increasing among Chinese patients, with challenges in diagnosis due to lack of specificity in clinical presentations and imaging findings. Diagnosis is primarily confirmed through histopathological examination, and the rarity of endemic mycoses along with limited availability of diagnostic tests poses challenges. The use of LCM and molecular techniques has shown improvement in species identification for timely diagnosis.
EMERGING MICROBES & INFECTIONS
(2021)
Article
Oncology
Kristina Navrazhina, Sandra Garcet, Samuel C. Williams, Nicholas Gulati, Felix Kiecker, John W. Frew, Hiroshi Mitsui, James G. Krueger
Summary: The study demonstrates the importance of using laser capture microdissection (LCM) to accurately identify molecular changes in melanoma samples, without being affected by inflammatory infiltration. LCM analysis identified a greater number of differentially expressed genes, including melanocyte-specific genes, and revealed pathways related to cell proliferation.
PIGMENT CELL & MELANOMA RESEARCH
(2023)
Article
Plant Sciences
Wei Qin, Yongpeng Li, Bowen Peng, Hang Liu, Tiantian Chen, Xin Yan, Yaojie Zhang, Chen Wang, Xinghao Yao, Xueqing Fu, Ling Li, Kexuan Tang
Summary: In this study, a method using laser capture microdissection (LCM) was developed to isolate glandular secretory trichomes (GSTs) from leaf epidermis without fixation. The method efficiently captured GSTs and enriched for related metabolites. This approach enables the study of pure plant cell populations and has the potential to discover novel secondary metabolites.
FRONTIERS IN PLANT SCIENCE
(2022)
Article
Biochemical Research Methods
Ganesh P. Pujari, Kiran K. Mangalaparthi, Benjamin J. Madden, Firdous A. Bhat, M. Cristine Charlesworth, Amy J. French, Gunveen Sachdeva, Eugenio Daviso, Ulrich Thomann, Patrick McCarthy, Sameer Vasantgadkar, Debadeep Bhattacharyya, Akhilesh Pandey
Summary: Lasercapture microdissection (LCM) is essential for proteomic analysis of specific regions from FFPE tissue samples. Automation of sample preparation workflows is still under development. This study presents a simplified and rapid workflow using adaptive focused acoustics (AFA) technology for high-throughput FFPE-based proteomics.
JOURNAL OF THE AMERICAN SOCIETY FOR MASS SPECTROMETRY
(2023)
Article
Plant Sciences
Liqing Lu, Haijian Yang, Yanhui Xu, Li Zhang, Juxun Wu, Hualin Yi
Summary: This study used RNA-seq and Pacbio-seq technologies to analyze the transcriptome and plant hormone signals in citrus seed development, revealing the important roles of hormone signaling, cell division, and nutrient metabolism processes. Additionally, several genes, including CrWRKY74, were found to play critical roles in seed abortion.
Article
Pharmacology & Pharmacy
Shashank Pandey, Zdenek Tuma, Tereza Smrhova, Miroslava Cedikova, Tereza Macanova, Magdalena Chottova Dvorakova
Summary: A platform for precise proteomic profiling of targeted cell populations from heterogeneous tissue sections is developed using LCM and CAP-IA integration, showing effectiveness for handling very small tissue sections.
Article
Biochemical Research Methods
Jongmin Woo, Madeline Schoenfeld, Xinguo Sun, Thierry Iraguha, Zhanxiang Zhou, Qibin Zhang
Summary: Using a simplified method, we conducted a proteomics study of intestinal tissue enriched with Paneth cells and identified several proteins associated with Paneth cell characteristics.
JOURNAL OF PROTEOME RESEARCH
(2022)
Article
Multidisciplinary Sciences
Xinjia Cai, Heyu Zhang, Jianyun Zhang, Tiejun Li
Summary: Oral submucous fibrosis (OSF) is a common potentially malignant disorder in the oral cavity, and researchers have proposed a method using laser capture microdissection to obtain epithelial and mesenchymal cells for studying the molecular mechanisms of this disease.
JOVE-JOURNAL OF VISUALIZED EXPERIMENTS
(2023)
Article
Biochemical Research Methods
Firat Kaya, Matthew D. Zimmerman, Rosleine Antilus-Sainte, Martin Gengenbacher, Claire L. Carter, Veronique Dartois
Summary: This study developed a workflow that combines laser-capture microdissection and mass spectrometry imaging to investigate the spatial distribution and quantitation of small molecule drugs in bones. The optimized staining methods enabled simultaneous histology staining and mass spectrometry imaging, revealing drug accumulation in bone tissue architecture.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2022)
Review
Biochemistry & Molecular Biology
Wenbo Guo, Yining Hu, Jingyang Qian, Lidan Zhu, Junyun Cheng, Jie Liao, Xiaohui Fan
Summary: Spatial omics technologies provide valuable insights into cells and tissues within a complex context, enhancing our understanding of complex biological systems. Laser capture microdissection (LCM) is an advanced method that quickly collects regions of interest (ROIs) from heterogeneous tissues, and is widely used for studying the cellular and molecular mechanisms of diseases.
JOURNAL OF GENETICS AND GENOMICS
(2023)
