Article
Biochemical Research Methods
Peipei Zhu, Xiaofeng Wu, Ruo-Yu Zhang, Chuan-Chih Hsu, Zhong-Yin Zhang, W. Andy Tao
Summary: Protein phosphatases are important in cellular processes, but studying their substrates and mechanisms is challenging. This study introduces a novel strategy to identify substrates of SHP2 using quantitative proteomics methods, and discovers new substrates.
JOURNAL OF PROTEOME RESEARCH
(2022)
Article
Biochemical Research Methods
Sergio Alonso-Fernandez, Ignacio Arribas-Diez, Gemma Fernandez-Garcia, Nathaly Gonzalez-Quinonez, Ole N. Jensen, Angel Manteca
Summary: This article describes the expansion of the Streptomyces phosphoproteome using optimized immobilized zirconium (IV) affinity chromatography and mass spectrometry, resulting in the identification of many novel phosphoproteins. The study enhances our understanding of Streptomyces development and secondary metabolism regulation.
JOURNAL OF PROTEOMICS
(2022)
Article
Nanoscience & Nanotechnology
Danqing Wang, Junfeng Huang, Haoran Zhang, Ting-Jia Gu, Lingjun Li
Summary: Protein phosphorylation is an important PTM involved in many cellular functions. This study developed a novel platform for phosphopeptide enrichment using a phosphorylated cotton-fiber-based Ti(IV)-IMAC material. The material showed high efficiency and selectivity in enriching phosphopeptides and demonstrated excellent sensitivity and specificity in complex biological samples.
ACS APPLIED MATERIALS & INTERFACES
(2023)
Article
Plant Sciences
Wasinee Pongprayoon, Atikorn Panya, Janthima Jaresitthikunchai, Narumon Phaonakrop, Sittiruk Roytrakul
Summary: This study aims to identify novel chitosan-responsive phosphoproteins in drought-sensitive rice cultivars LPT123 and KDML105 and compare their responses to chitosan treatment. The results showed that 22 proteins had different expression levels in response to chitosan treatment before osmotic stress.
Review
Biochemistry & Molecular Biology
Olga Shevchuk, Antonija Jurak Begonja, Stepan Gambaryan, Matthias Totzeck, Tienush Rassaf, Tobias B. Huber, Andreas Greinacher, Thomas Renne, Albert Sickmann
Summary: This review discusses the role of proteomics in understanding the molecular basics of traditional and emerging functions of platelets, as well as the application of proteomic technologies in platelet biogenesis, signaling, and storage. Additionally, the potential of newly appeared trapped ion mobility spectrometry (TIMS) in platelet research is highlighted, along with discussions on the implementation of proteomic methods in platelet transfusion medicine, diagnostics, and prognostics.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Pharmacology & Pharmacy
Tigran Margaryan, Mackenna Elliott, Nader Sanai, Artak Tovmasyan
Summary: A sensitive and rapid LC-MS/MS method was developed for quantifying LY3214996, abemaciclib, and their metabolites in human samples. The method was successfully applied in a clinical trial.
JOURNAL OF PHARMACEUTICAL ANALYSIS
(2022)
Article
Chemistry, Analytical
Xuefei Zhong, Shruti Nayak, Lili Guo, Shivkumar Raidas, Yunlong Zhao, Rachel Weiss, Matthew Andisik, Chinnasamy Elango, Giane Sumner, Susan C. Irvin, Michael A. Partridge, Hong Yan, Sook E. Yen, Haibo Qiu, Yuan Mao, Albert Torri, Ning Li
Summary: REGEN-COV, a cocktail of two human monoclonal antibodies, has shown promising results in reducing SARS-CoV-2 viral load in COVID-19 patients. A liquid chromatography-mass spectrometry method was developed for the determination of REGEN-COV concentrations in serum samples from patients participating in clinical trials. The concentrations measured by this method were comparable to those measured by a validated immunoassay.
ANALYTICAL CHEMISTRY
(2021)
Article
Toxicology
Xing Ma, Xiaowei Yu, Jialing Min, Xin Chen, Ren Liu, Xueqing Cui, Jing Cheng, Mingyong Xie, Patrick Diel, Xiaojuan Hu
Summary: In this study, it was found that oral exposure to genistein (GEN) at a level comparable to dietary intake in humans could interfere with the antitumor effects of cisplatin (CIS) chemotherapy, possibly by blocking cell proliferation inhibition and apoptosis induction. Furthermore, higher concentrations of GEN were observed in xenograft tumor tissues compared to serum and liver.
TOXICOLOGY LETTERS
(2022)
Article
Chemistry, Analytical
Alan B. Moran, Richard A. Gardner, Manfred Wuhrer, Guinevere S. M. Lageveen-Kammeijer, Daniel I. R. Spencer
Summary: Sialic acids play diverse biological roles and linkage-specific analysis is important in glycomic studies. The combination of procainamide fluorescent labeling with sialic acid linkage-specific derivatization is presented, which allows for unambiguous assignment of sialic acid linkages and separation of sialylated isomers.
ANALYTICAL CHEMISTRY
(2022)
Article
Agronomy
Archis Pandya, Bjorn Thiele, Andres Zurita-Silva, Bjorn Usadel, Fabio Fiorani
Summary: The study evaluated saponin content and their corresponding sapogenins in 114 different quinoa accessions, showing considerable variability in saponin content in quinoa. Approximately 75% of the genotypes were classified as low-saponin content lines, which is promising for current and future quinoa breeding programs. Additionally, twelve saponins of the oleanane type were identified, including a previously unreported compound, contributing to the valorization of genetic resources for quinoa.
Review
Biochemical Research Methods
Jonas Carneiro Cruz, Israel Donizeti de Souza, Fernando Mauro Lancas, Maria Eugenia Costa Queiroz
Summary: Miniaturized liquid chromatography (LC) is recognized as an important analytical method in various research fields, offering higher resolution and lower consumption. However, limitations such as tubing clogging and small sample volumes hinder high throughput and sensitive analyses. Online coupling of sample preparation techniques can overcome these limitations and improve analytical efficiency.
JOURNAL OF CHROMATOGRAPHY A
(2022)
Article
Chemistry, Analytical
Linlin Dong, Nicole Bebrin, Konstantin Piatkov, Kojo Abdul-Hadi, Shinji Iwasaki, Mark G. Qian, Dong Wei
Summary: In the field of immuno-oncology, a highly sensitive and specific bioanalytical method is required for quantifying surrogate mouse antibodies, and the immunoaffinity enrichment based LC/MS/MS assay may provide an effective solution by differentiating analyte response from high endogenous immunoglobulin background.
ANALYTICAL CHEMISTRY
(2021)
Article
Multidisciplinary Sciences
Hongjiang Xu, Yanan Li, Jing Zhang, Jinhua Zhang, Jianguang Lu, Xiquan Zhang, Ling Yang, Wenjie Zhao, Jun Feng
Summary: A rapid UHPLC-MS/MS assay method was developed for the determination of CMS E2 and colistin E2 in human plasma. The method demonstrated speed and high accuracy, and was successfully applied to the analysis of plasma samples obtained from Chinese healthy volunteers.
SCIENTIFIC REPORTS
(2023)
Article
Environmental Sciences
Yan Wu, Gillian Z. Miller, Jeff Gearhart, Graham Peaslee, Marta Venier
Summary: In 2018, popular children car seats in the United States were tested for fluorine content and PFAS. Results showed that PFAS were detected in 97% of the samples with total concentrations up to 268 ng/g, and fabric samples generally had higher levels than foam and laminated composites. Furthermore, the presence of side-chain fluorotelomer-based polymers and fluorotelomer (meth)acrylates indicated the potential degradation into PFAAs under UV light, highlighting a possible dermal route of exposure.
ENVIRONMENTAL POLLUTION
(2021)
Article
Pharmacology & Pharmacy
Geoffrey A. Walford, Kelly E. Duncan, Moises Hernandez, Pavan Vaddady, Marcus Hompesch, Linda Morrow, S. Aubrey Stoch
Summary: MK-1092, a synthetic insulin dimer, has favorable properties and demonstrated significant effects in comparison to conventional insulins. The trial conducted in healthy adults and diabetes patients showed that MK-1092 was well tolerated with dose-proportional pharmacodynamic measurements.
CLINICAL PHARMACOLOGY & THERAPEUTICS
(2022)