Article
Biochemistry & Molecular Biology
Dmitry Senko, Anna Gorovaya, Elena Stekolshchikova, Nickolay Anikanov, Artur Fedianin, Maxim Baltin, Olga Efimova, Daria Petrova, Tatyana Baltina, Mikhail A. Lebedev, Philipp Khaitovich, Anna Tkachev
Summary: The study assessed blood lipidome alterations in a rat sciatic nerve crush injury model and found delayed systematic alterations in fatty acid metabolism after nerve injury.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biology
Jan Eckhardt, Alexis Ruiz, Stephane Koenig, Maud Frieden, Herve Meier, Alexander Schmidt, Susan Treves, Francesco Zorzato
Summary: Skeletal muscles are responsible for movement and metabolic regulation. Congenital myopathies, caused by mutations in genes such as RYR1, lead to weak muscles. This study found that RYR1 mutations decrease RyR1 protein content and alter the expression of proteins involved in calcium signaling, metabolism, and ER protein quality control. It also identified potential targets for treating RyR1-related congenital myopathies.
Review
Biochemistry & Molecular Biology
Isabelle Alldritt, Paul L. Greenhaff, Daniel J. Wilkinson
Summary: This article highlights the impact of muscle deconditioning on locomotor function and metabolic health, as well as the potential role of metabolomics in studying muscle decline. By analyzing the metabolite composition within cells, metabolomics can uncover the pathophysiological characteristics of muscle mass loss and deconditioning.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Geriatrics & Gerontology
Marco Narici, Jamie McPhee, Maria Conte, Martino V. Franchi, Kyle Mitchell, Sara Tagliaferri, Elena Monti, Giuseppe Marcolin, Philip J. Atherton, Kenneth Smith, Bethan Phillips, Jonathan Lund, Claudio Franceschi, Marcello Maggio, Gillian S. Butler-Browne
Summary: The study introduced an ultrasound imaging method for diagnosing sarcopenia based on changes in muscle geometric proportions. The ultrasound sarcopenic index (USI) was proposed as a novel marker for the loss of muscle mass associated with sarcopenia, providing a useful 'signature of sarcopenia' and allowing for stratification of individuals according to muscle sarcopenia presence and severity. The USI was found to be a useful clinical tool for confirming the diagnosis of sarcopenia, with DXA-derived skeletal muscle index (SMI) showing significant correlation with USI.
JOURNAL OF CACHEXIA SARCOPENIA AND MUSCLE
(2021)
Article
Biochemistry & Molecular Biology
Liangfu Jiang, Xingyu Ouyang, Dupiao Zhang, Gang Wang, Zhe Zhang, Wei Wang, Hede Yan
Summary: This study presents a Gel-Ppy modified nerve conduit with excellent biocompatibility and conductivity, and evaluates its performance in promoting nerve regeneration in vitro and in vivo. The results demonstrate that Gel-Ppy modified nerve conduit can significantly enhance Schwann cell proliferation, contribute to Schwann cell regeneration in vivo, and improve axon diameters and myelin sheath thickness, leading to the amelioration of muscle atrophy, nerve conduction, and motor function recovery. Further investigation indicates that the Gel-Ppy conduit facilitates nerve regeneration by upregulating the Rap1 pathway to induce neurite outgrowth. Therefore, Gel-Ppy modified nerve conduit can provide a favorable microenvironment for nerve regeneration and be popularized as a novel therapeutic strategy for peripheral nerve injury.
Article
Cell & Tissue Engineering
Benjamin K. Schilling, Jocelyn S. Baker, Chiaki Komatsu, Kacey G. Marra
Summary: Peripheral nerve injury and muscle atrophy have a significant healthcare burden in the United States, partly due to the lack of specific preventative therapies. Injecting skeletal muscle-derived ECM directly into denervated muscle can promote muscle regeneration and improve muscle function.
JOURNAL OF TISSUE ENGINEERING
(2021)
Article
Biochemistry & Molecular Biology
Zeyad H. Nafaee, Balint Hajdu, Eva Hunyadi-Gulyas, Bela Gyurcsik
Summary: The nuclease domain of colicin E7 can cleave DNA non-specifically. In this study, it was found that Zn2+ ion is the native metal ion of the enzyme and cannot be replaced by other metal ions such as Ni2+, Cu2+ and Cd2+. The binding of Ni2+ ions significantly increases the nuclease activity of the enzyme, while the presence of equivalent Zn2+ ions decreases the activity.
Article
Biology
Jose Angel Huerta-Ocampo, Lino Gerardo Batista-Roche, Martha Beatriz Morales-Amparano, Maria del Refugio Robles-Burgueno, Gabriela Ramos-Clamont Montfort, Luz Vazquez-Moreno, Fernando Ramirez-Jimenez, Luis M. Teran
Summary: Velvet mesquite, a native legume of the southwestern United States and northwestern Mexico, plays a key role in the desert ecosystem but is also a major cause of allergic respiratory disease. This study identified allergenic proteins in velvet mesquite pollen, which will contribute to the development of specific diagnosis and treatment for mesquite pollen allergy.
Article
Physiology
Ryan Neil Marshall, James McKendry, Benoit Smeuninx, Alex Peter Seabright, Paul T. Morgan, Carolyn Greig, Leigh Breen
Summary: Ageing is associated with changes in skeletal muscle oxidative metabolism, which may be influenced by physical activity status. The effect of resistance exercise training on skeletal muscle mitochondrial regulation is unclear.
FRONTIERS IN PHYSIOLOGY
(2023)
Article
Cell Biology
Agnieszka U. Blachnio-Zabielska, Kamila Roszczyc-Owsiejczuk, Monika Imierska, Karolina Pogodzinska, Pawel Rogalski, Jaroslaw Daniluk, Piotr Zabielski
Summary: Skeletal muscle lipid metabolism plays a crucial role in insulin resistance, and the accumulation of ceramides is considered to be one of the causes. The study found that the accumulation of C18:0-Cer plays a key role in inducing skeletal muscle insulin resistance.
Article
Food Science & Technology
Welder Baldassini, Mohammed Gagaoua, Bismarck Santiago, Leone Rocha, Juliana Torrecilhas, Rodrigo Torres, Rogerio Curi, Otavio Machado Neto, Pedro Padilha, Felipe Santos, Dante Pazzanese Lanna, Luis Artur Chardulo
Summary: This study evaluated the impact of feeding wet distiller grains (WDG) on meat quality and protein expression in beef cattle. The results showed that WDG-fed cattle had larger ribeye area and higher carcass weight compared to the control group, while intramuscular fat content decreased. Proteomic analysis revealed significant changes in protein expression and various biological processes and pathways. However, WDG supplementation did not compromise meat tenderness and fatty acid profile.
Article
Neurosciences
Elisabetta Indelicato, Klaus Faserl, Matthias Amprosi, Wolfgang Nachbauer, Rainer Schneider, Julia Wanschitz, Bettina Sarg, Sylvia Boesch
Summary: This study used proteomics analysis to compare muscle samples from FRDA patients and controls, and found significant differences in mitochondrial protein profiles and metabolic pathways. These findings have important implications for the design of future therapeutic strategies.
FRONTIERS IN NEUROSCIENCE
(2023)
Article
Biochemistry & Molecular Biology
Zhengye Liu, Thomas Chaillou, Estela Santos Alves, Theresa Mader, Baptiste Jude, Duarte M. S. Ferreira, Heidi Hynynen, Arthur J. Cheng, William O. Jonsson, Gianluigi Pironti, Daniel C. Andersson, Ellinor Kenne, Jorge L. Ruas, Pasi Tavi, Johanna T. Lanner
Summary: The study demonstrates that NDUFA4L2 regulates skeletal muscle mass and force by decreasing mitochondrial activity and production of reactive oxygen species, leading to muscle atrophy. Overexpression of NDUFA4L2 reduces mitochondrial respiration and antioxidant capacity, lowering levels of important intramuscular metabolites, ultimately affecting muscle function.
Review
Biochemistry & Molecular Biology
Jason Tallis, Sharn Shelley, Hans Degens, Cameron Hill
Summary: Obesity and aging have negative impacts on skeletal muscle function, with their combined effects yet to be thoroughly explored. Poor muscle function is associated with chronic diseases, poor quality of life, and all-cause mortality, highlighting the importance of studying and intervening in these effects for overall health and physical function.
Article
Health Care Sciences & Services
Jung Hae Yun, Du Hwan Kim, Min Cheol Chang
Summary: This study demonstrated that simple bedside exercise training for moderate-degree AD patients can significantly increase maximal voluntary contraction for hip flexion and knee extension, helping to maintain and enhance lower extremity muscle strength.
Article
Biochemistry & Molecular Biology
Pietro Barbacini, Enrica Torretta, Beatrice Arosio, Evelyn Ferri, Daniele Capitanio, Manuela Moriggi, Cecilia Gelfi
Summary: Sphingolipids play an important role in aging and various disorders. This study analyzed the distribution of sphingolipid species and enzyme levels associated with centenarians' decelerated aging. The results showed that centenarians had a specific profile of sphingolipids and a fine-tuning mechanism, indicating a protective mechanism against physiological decline.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Daniele Capitanio, Manuela Moriggi, Pietro Barbacini, Enrica Torretta, Isabella Moroni, Flavia Blasevich, Lucia Morandi, Marina Mora, Cecilia Gelfi
Summary: BMD patients were divided into two groups, BMD1 and BMD2, with BMD2 showing increased levels of proteins characteristic of fast fibers and proteins related to sarcomere reorganization. In BMD2, there was an increase in glycolysis, gluconeogenesis, and proper TCA cycle flux, enabling maintenance of metabolism and epithelial adherens junctions. Additionally, BMD2 exhibited elevated levels of acetylated proteoforms of moonlighting proteins, promoting stem cell recruitment and muscle regeneration.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Chemistry, Multidisciplinary
Chiara Fania, Marco Bagnati, Marina Albertario, Carlotta Ferraris, Marta Lamonaca, Umberto Dianzani
Summary: The analysis of immunosuppressant drugs using liquid chromatography coupled to tandem mass spectrometry (LC-MS/MS) is considered the gold standard. Automated systems, such as the Thermo Scientific(TM) Cascadion(TM) SM Clinical Analyzer, eliminate manual processes and provide efficient and accurate results. The comparison of LC-MS/MS with the automated system showed a good correlation between the two methods.
APPLIED SCIENCES-BASEL
(2022)
Article
Medicine, General & Internal
Enrico Ragni, Marco Vigano, Enrica Torretta, Carlotta Perucca Orfei, Alessandra Colombini, Carlo Tremolada, Cecilia Gelfi, Laura de Girolamo
Summary: The use of microfragmented adipose tissue (mu FAT) for the treatment of musculoskeletal disorders, especially osteoarthritis, is gaining popularity. This study characterized the structure and cell content of unprocessed lipoaspirate (LA) and mu FAT, and identified the array of signaling factors in the context of osteoarthritis. The results showed that mu FAT had reduced blood cells and maintained tissue structure and stromal cells, with increased molecular features beneficial for osteoarthritis.
JOURNAL OF CLINICAL MEDICINE
(2022)
Article
Cell Biology
Vincenzo La Mura, Nicoletta Gagliano, Francesca Arnaboldi, Patrizia Sartori, Patrizia Procacci, Luca Denti, Eleonora Liguori, Niccolo Bitto, Giuseppe Ristagno, Roberto Latini, Daniele Dondossola, Francesco Salerno, Armando Tripodi, Massimo Colombo, Flora Peyvandi
Summary: This study investigated the hemostatic changes induced by endotoxemia and the protective effects of simvastatin on liver vascular microthrombosis. The results showed that simvastatin prevented fibrin deposition and hypo-coagulability caused by endotoxemia, and preserved the antithrombotic properties of sinusoidal endothelial cells. It suggests that simvastatin has potential pharmacological properties to counteract sepsis-associated coagulopathy and hepatic failure.
Article
Cell Biology
Pietro Barbacini, Dieter Blottner, Daniele Capitanio, Gabor Trautmann, Katharina Block, Enrica Torretta, Manuela Moriggi, Michele Salanova, Cecilia Gelfi
Summary: Physical inactivity or prolonged bed rest can lead to muscle deconditioning and increased cardiovascular disease risk. Nutritional interventions, such as an antioxidant cocktail, have been found to preserve lipemic and reactive oxidative and nitrosative stress (RONS) homeostasis. This study investigated the effects of an antioxidant cocktail on serum molecules in healthy male subjects enrolled in a 60-day bed rest protocol.
Article
Biochemistry & Molecular Biology
Viviana Tritto, Daniele Capitanio, Cecilia Gelfi, Paola Riva
Summary: Noonan syndrome is a multisystem disorder caused by mutations in RAS pathway genes. In some cases, molecular diagnosis is not possible, suggesting additional unknown genes or mechanisms. A study on two NS patients showed that the presence of subclinical variants from both parents can activate the RAS pathway and potentially contribute to the development of NS.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biochemistry & Molecular Biology
Dieter Blottner, Manuela Moriggi, Gabor Trautmann, Maria Hastermann, Daniele Capitanio, Enrica Torretta, Katharina Block, Joern Rittweger, Ulrich Limper, Cecilia Gelfi, Michele Salanova
Summary: This study analyzed the muscle adaptation to spaceflight in male International Space Station (ISS) astronauts. The results showed that long-duration mission (LDM) astronauts who performed routine inflight exercise had moderate myofiber atrophy compared to short-duration mission (SDM) astronauts, suggesting the importance of exercise in maintaining muscle health during spaceflight. These findings contribute to the understanding of skeletal muscle adaptation and provide valuable information for future deep space exploration.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)