Review
Chemistry, Analytical
Didem Nur Unal, Sercan Yildirim, Sevinc Kurbanoglu, Bengi Uslu
Summary: Surfactants are frequently used in different fields of analytical chemistry to reduce the interfacial tension of immiscible liquids by forming micelles through hydrophobic and hydrophilic groups. Their use in techniques such as chromatography and electrochemistry has been noted for improving separation efficiency and increasing sensor selectivity and sensitivity.
TRAC-TRENDS IN ANALYTICAL CHEMISTRY
(2021)
Article
Chemistry, Analytical
Yating Lu, Yue Guo, Xiao Liang, Huimin Huang, Xue Ling, Zhiheng Su, Yonghong Liang
Summary: In this study, gold nanoclusters with strong red fluorescence were successfully synthesized using bovine serum albumin as a reducing agent and stabilizer, for the rapid and effective detection of AAI. The detection results were consistent with the traditional HPLC method, demonstrating a promising application potential.
ANALYTICAL METHODS
(2022)
Review
Chemistry, Medicinal
Hussain Alessa, Amr L. Saber, Jalal T. Althakafy
Summary: This study aimed to summarize recent outcomes of chromatographic methods, such as gas chromatography and high-performance liquid chromatography, used for the determination of melatonin over the past three decades.
ARCHIV DER PHARMAZIE
(2022)
Article
Immunology
Rahul Basu, Vinod Nair, Clayton W. Winkler, Tyson A. Woods, Iain D. C. Fraser, Karin E. Peterson
Summary: The study found that BCECs isolated from adult and weanling mice have differential viral load, infectivity, and susceptibility to LACV. These age-related differences in susceptibility may strongly influence LACV-induced BBB leakage and neurovascular damage allowing virus invasion of the CNS and the development of neurological disease.
JOURNAL OF NEUROINFLAMMATION
(2021)
Article
Chemistry, Analytical
Gege Song, Zhiqiao Zou, Ailing Feng, Chenbiao Li, Meijuan Liang, Fuan Wang, Xiaoqing Liu
Summary: In this study, a flexible and portable light-up aptameric sensor was developed for smartphone-based point-of-care testing. By manipulating the affinity and dynamic response of designer DNA molecules, detection of serotonin was achieved with high sensitivity and selectivity. The study also demonstrated the versatility of the engineered aptameric molecular probes for different sensing applications.
ANALYTICAL CHEMISTRY
(2023)
Article
Chemistry, Analytical
Xinglei Zhang, Yuan Xu, Ting Li, Hui Li, Zhendong Yu, Lili Song, Jiaquan Xu
Summary: In this study, electrochemical mass spectrometry (EC-MS) combined with in situ electrospray ionization mass spectrometry (ESI-MS) was used to directly analyze heavy metal speciations in suspended particulate matter (SPM) in water. Four types of speciations were obtained through water and methanol extraction and sequential extraction. Compared with traditional methods, this method has the advantages of no complex matrix separation process, less sample consumption, and high analysis throughput, making it suitable for advanced applications in environmental monitoring and risk assessment.
JOURNAL OF ANALYTICAL ATOMIC SPECTROMETRY
(2023)
Article
Biochemical Research Methods
Henri Ravald, Susanne K. Wiedmer
Summary: This article describes the use of lipid vesicles and liposomes as cell membrane models for investigating interactions between lipid membranes and 0-blockers in capillary electromigration and liquid chromatography techniques. The use of commercial intravenous lipid emulsions and their interactions with 0-blockers are also discussed. Different instrumental techniques for these purposes are introduced, and other methodologies for studying 0-blocker interactions with lipid membranes are compared.
JOURNAL OF CHROMATOGRAPHY A
(2023)
Review
Chemistry, Analytical
Brenda Lee Simas Porto, Alberto Valdes, Alejandro Cifuentes, Gerardo Alvarez-Rivera
Summary: This article revisits recent articles that have used capillary electromigration techniques to study various phytochemicals between 2018 and 2021. The manuscript is divided based on the different separation methods used, and the main aspects of each application are briefly discussed, including sample preparation, advantages and disadvantages of the developed methods, problems encountered during the process, and ways to solve them.
TRAC-TRENDS IN ANALYTICAL CHEMISTRY
(2023)
Article
Biochemical Research Methods
Mohamed Adel Ahmed, Joselito P. Quirino
Summary: The study discusses the in-line sample concentration by micelle to cyclodextrin stacking in open-tubular liquid chromatography with UV detection. Factors influencing MCDS, such as type and concentration of CD, concentration of CTAB, were studied to achieve higher sensitivity and linear range under optimized conditions. Application to real samples showed comparable results to high-performance liquid chromatography-UV method.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2022)
Review
Chemistry, Analytical
Albert Perez-Alcaraz, Francesc Borrull, Marta Calull, Carme Aguilar
Summary: The consumption of synthetic cathinones has been increasing in recent years due to its affordability and accessibility as an alternative to amphetamines. Developing procedures capable of detecting and monitoring their consumption is crucial, and urine has been widely used for this purpose as it is non-invasive and provides larger sample volumes than other biological matrices. Chromatographic techniques are the most reported approach for determining cathinones in urine samples.
TRAC-TRENDS IN ANALYTICAL CHEMISTRY
(2021)
Review
Food Science & Technology
Huynh N. P. Dang, Joselito P. Quirino
Summary: Alkenylbenzenes are potentially toxic compounds found in food, especially in flavorings, requiring constant monitoring due to concerns about their health effects. The challenge in determining alkenylbenzenes in food lies in the complex sample matrices and low amounts present. Gas and liquid chromatography are commonly used separation techniques, with detection methods including flame ionisation and mass spectrometry.
Review
Chemistry, Inorganic & Nuclear
Rehab H. Elattar, Samah F. El-Malla, Amira H. Kamal, Fotouh R. Mansour
Summary: Metal complexes have strong analysis potential and can be used to measure analytes lacking essential chemical properties. Their analytical methods based on different mechanisms offer superior performance.
COORDINATION CHEMISTRY REVIEWS
(2024)
Article
Chemistry, Analytical
Cynthia Nagy, Anna Pesti, Melinda Andrasi, Gabor Vasas, Attila Gaspar
Summary: The study investigated the determination of artemisinin and its analogs using MEKC coupled with mass spectrometric detection. It was found that in addition to artemisinin, a variety of other organic components could be separated and determined in the ethanolic extracts of Artemisia annua leaves. Furthermore, diastereomers of several compounds were identified in the plant extracts using MEKC-MS.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2021)
Article
Chemistry, Analytical
Raffeal Bennett, Ryan D. Cohen, Heather Wang, Tony Pereira, Mark A. Haverick, John W. Loughney, Damon C. Barbacci, Pavlo Pristatsky, Amy M. Bowman, Gioacchino Luca Losacco, Douglas D. Richardson, Ian Mangion, Erik L. Regalado
Summary: This study presents an automated high-throughput method for EDTA detection, utilizing EDTA esterification and ultra-high-performance liquid chromatography-triple quadrupole tandem mass spectrometry, which offers high sensitivity, excellent performance, and an automation-friendly workflow.
ANALYTICAL CHEMISTRY
(2022)
Review
Chemistry, Analytical
Apostolia Tsiasioti, Constantinos K. Zacharis, Paraskevas D. Tzanavaras
Summary: This article reviews recent studies on the determination of histamine in food matrices using derivatization-based separation methods. The derivatization of histamine plays an important role in enhancing sensitivity, selectivity, and compatibility to analytical techniques. The article also discusses the application of the latest derivatizing reagents for histamine and various analytical techniques.
TRAC-TRENDS IN ANALYTICAL CHEMISTRY
(2023)
Article
Food Science & Technology
Barbara Deracinois, Aurelie Mateos, Audrey Romelard, Audrey Boulier, Julie Auger, Alain Baniel, Rozenn Ravallec, Christophe Flahaut
Summary: A four-phase strategy was proposed to enhance the identification of phosphopeptides and phosphosites, resulting in the successful identification of 90% of phosphorylation sites in the analyzed hydrolysates after treatment.
Article
Cell Biology
Camille Menaceur, Fabien Gosselet, Laurence Fenart, Julien Saint-Pol
Summary: The construction and maintenance of the blood-brain barrier (BBB) relies on cell-cell communication processes between endothelial cells, pericytes, glial cells (mainly astrocytes), and neurons. Understanding these communication pathways is crucial for studying the physiology and pathophysiology of the BBB.
Review
Food Science & Technology
Mathie Tenenbaum, Barbara Deracinois, Camille Dugardin, Aurelie Mateos, Audrey Romelard, Julie Auger, Audrey Boulier, Rozenn Ravallec, Christophe Flahaut, Benoit Cudennec
Summary: Milk and dairy products are important sources of proteins and peptides that have significant impact on human health. CPPs, bioactive phosphorylated peptides resulting from the hydrolysis of caseins, have attracted increasing interest due to their ability to chelate minerals and increase their bioavailability, as well as their antioxidant and anti-inflammatory properties. This review discusses the analytical characterization, release during gastrointestinal digestion, obtention processes, physicochemical characteristics, and bioactive roles of CPPs. It highlights the potential of CPPs in treating various pathologies and the need for further research to elucidate their mechanisms of action and potential for human health.
FOOD RESEARCH INTERNATIONAL
(2022)
Article
Microbiology
Elodie Dussert, Melissa Tourret, Chloe Dupuis, Alexandre Noblecourt, Josette Behra-Miellet, Christophe Flahaut, Rozenn Ravallec, Francois Coutte
Summary: This study investigated the antiradical and antioxidant potential of lipopeptides produced by Bacillus subtilis strains. The results showed that plipastatin exhibited the highest antioxidant activities.
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Pharmacology & Pharmacy
Desire Madi-Moussa, Barbara Deracinois, Radja Teiar, Yanyan Li, Marius Mihasan, Christophe Flahaut, Sylvie Rebuffat, Francoise Coucheney, Djamel Drider
Summary: This study reveals that the bacteriocin lacticaseicin 30, a rare Gram-positive bacteriocin that inhibits Gram-negative bacteria, requires at least two helices in the N-terminal region and carries amino acids in the C-terminal region that play a role in modulating its activity. These findings are crucial for designing lacticaseicin 30 variants as potential therapeutic agents against Gram-negative bacilli infections.
Article
Biochemistry & Molecular Biology
Anne-Sophie Paguet, Ali Siah, Gabriel Lefevre, Sophie Moureu, Thierry Cadalen, Jennifer Samaillie, Franck Michels, Barbara Deracinois, Christophe Flahaut, Harmony Alves Dos Santos, Audrey Etienne-Debaecker, Caroline Rambaud, Sylvie Chollet, Roland Molinie, Jean-Xavier Fontaine, Christophe Waterlot, Marie-Laure Fauconnier, Sevser Sahpaz, Celine Riviere
Summary: This study investigates the genetic and chemical diversity of fifty wild hops collected from different locations in Northern France. The results show that wild hops exhibit high levels of genetic and chemical diversity, making them an important resource for the species.
Article
Food Science & Technology
Fatma Hamed, Imen Elgaoud, Barbara Deracinois, Christophe Flahaut, Naima Nedjar, Ahmed Barkia
Summary: In this study, Diplodus proteins were hydrolyzed using alcalase and savinase, and the hydrolysates were characterized for their chemical composition, functional properties and peptide content. The hydrolysates produced with savinase had a higher protein content and were richer in hydrophobic peptides compared to those produced with alcalase. Savinase hydrolysate also showed better solubility, foaming properties, and oil and water retention capacities, while alcalase hydrolysate had better emulsifying property and emulsion stability. These results suggest that alcalase and savinase can be used to convert Diplodus annularis proteins into peptides with promising functional properties.
Article
Cell Biology
Marie-Pierre Dehouck, Masanori Tachikawa, Yutaro Hoshi, Kotaro Omori, Claude-Alain Maurage, Guillaume Strecker, Lucie Dehouck, Marie-Christine Boucau, Yasuo Uchida, Fabien Gosselet, Tetsuya Terasaki, Yannis Karamanos
Summary: This study compared the protein expression levels of human brain-like endothelial cells (hBLECs) and human brain microvessels (hBMVs) using quantitative targeted absolute proteomics. The results showed that hBLECs had higher protein levels of CD144, CD147, MRP4, Annexin A6, and caveolin-1 compared to hBMVs, while the protein levels of MCT1, Connexin 43, TfR1, and claudin-5 were relatively lower. Furthermore, BCRP was found to be more expressed than MDR1 in hBLECs. These findings contribute to a better understanding of the characteristics of hBLECs and demonstrate the equivalence of in vitro BBB models with in vivo models in terms of transport properties.
Article
Cell Biology
Camille Menaceur, Johan Hachani, Shiraz Dib, Sophie Duban-Deweer, Yannis Karamanos, Fumitaka Shimizu, Takashi Kanda, Fabien Gosselet, Laurence Fenart, Julien Saint-Pol
Summary: This study investigates the proteome differences between brain pericytes (BPs) cultured alone (hBP-solo) and in co-culture with brain microvessel endothelial cells (ECs) (hBP-coc) to model the human blood-brain barrier (BBB) in vitro. The results show that hBP-solo has 51 proteins related to cell proliferation, contractility, adhesion, and extracellular matrix elements, indicating an immature cell state. On the other hand, hBP-coc has 90 proteins associated with reduced contractile activities and enhanced metabolic functions, particularly in mitochondrial activities and sterol metabolism, suggesting mature pericytes. This study highlights the role of ECs in promoting the maturation and metabolic switch of BPs during barriergenesis.
Article
Food Science & Technology
Nathalie Atallah, Claire Gaudichon, Audrey Boulier, Alain Baniel, Benoit Cudennec, Barbara Deracinois, Rozenn Ravallec, Christophe Flahaut, Dalila Azzout-Marniche, Nadezda Khodorova, Martin Chapelais, Juliane Calvez
Summary: The aim of this study was to analyze the protein digestibility and postprandial metabolism in rats of milk protein matrices obtained by different industrial processes. The results showed that ingestion of whey proteins led to higher protein content in the intestinal mucosa and liver, possibly due to fast assimilation. Additionally, industrial processes that modify the structure and/or composition of milk proteins influence protein digestion and utilization.
FOOD RESEARCH INTERNATIONAL
(2023)
Article
Biochemistry & Molecular Biology
Ahlam Outman, Barbara Deracinois, Christophe Flahaut, Mira Abou Diab, Bernard Gressier, Bruno Eto, Naima Nedjar
Summary: Human hemoglobin and bovine hemoglobin both contain rich bioactive peptides, with high similarity between the two. Bioinformatics tools and enzymatic hydrolysis experiments revealed the presence of various bioactive peptides, including some that have never been found in human hemoglobin before.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biochemistry & Molecular Biology
Ahlam Outman, Barbara Deracinois, Christophe Flahaut, Mira Abou Diab, Jihen Dhaouefi, Bernard Gressier, Bruno Eto, Naima Nedjar
Summary: This study focuses on the enzymatic hydrolysis of hemoglobin and compares the antibacterial and antioxidant potentials of human and bovine hemoglobin hydrolysates. The results show strong antimicrobial and antioxidant activities of the peptide hydrolysates, independent of the initial substrate concentration level. The presence of bioactive peptides in human hemoglobin suggests its potential as a valuable source for the food or pharmaceutical industries.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Food Science & Technology
Mouna Ambli, Barbara Deracinois, Anne-Sophie Jenequin, Rozenn Ravallec, Benoit Cudennec, Christophe Flahaut
Summary: Bioinformatics software is essential in peptidomics/proteomics research, enabling the identification of peptides through the comparison of peptide fragmentation spectra obtained by mass spectrometry with targeted databases or by de novo sequencing. Customized software programming is crucial in improving peptide and post-translational modification (PTM) identification rates in the agrofood field.
Article
Biochemistry & Molecular Biology
Lea Fleury, Barbara Deracinois, Camille Dugardin, Alice B. Nongonierma, Richard J. FitzGerald, Christophe Flahaut, Benoit Cudennec, Rozenn Ravallec
Summary: This study compared the DPP-IV inhibitory potential of seven proteins from different sources in vitro and in rat plasma, revealing the impact of protein origin on their in vivo and in vitro DPP-IV inhibitory activity. The results indicated that vegetable proteins and hemoglobin exhibited the highest DPP-IV inhibitory activity.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)