期刊
PROTEINS-STRUCTURE FUNCTION AND BIOINFORMATICS
卷 71, 期 3, 页码 1057-1065出版社
WILEY
DOI: 10.1002/prot.21969
关键词
human insulin aggregates; diffusion coefficients; PFGSE NMR; oligomer distribution; association constants
The aggregation of Zn(II)bound and zinc-free human insulin was studied in solution using the H-beta similar to CH3 cross-peaks of threonine residues in 2D COSY, TOCSY, and NOESY NMR spectra which allow viewing of the oligomers in equilibrium. This is complemented by PFGSE measurements of the translational diffusion coefficient, D-i, used for monitoring the changes in equilibrium composition of aggregates on dilution of both insulins in physiological medium. The back calculation of the dilution isotherm allows establishing the association constants for oligomeric equilibria in solution and discussion of the models of association.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据