期刊
PROTEINS-STRUCTURE FUNCTION AND BIOINFORMATICS
卷 72, 期 1, 页码 173-183出版社
WILEY
DOI: 10.1002/prot.21905
关键词
short chain dehydrogenase; tropinone reductase; N-terminus; C-terminus; substrate affinity; substrate turnover; tropane alkaloid; calystegine; Solanum dulcamara
Unusual kinetic characteristics of tropinone reductase, an enzyme in the family of short chain dehydrogenases, prompted to investigate a possible impact of the hexahistidine affinity tag on catalytic properties. Comparison of enzymes from Solanum dulcamara, Solanaceae, tagged at the N-terminus or at the C-terminus revealed that the C-terminally tagged form was functionally impaired. Protein modeling indicated that the hexahistidine tag attached at the C-terminus but not at the N-terminus of the polypeptide can interfere with the active site by steric or electrostatic interactions. In consequence, protein modeling is suggested before enzyme expression with affinity tags to estimate possible interactions of affinity tags with the active center.
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