Article
Multidisciplinary Sciences
Lin Wang, Xingxing Yan, Yanjun Li, Zhiye Wang, Shweta Chhajed, Baoshuan Shang, Zhen Wang, Suk Won Choi, Hongwei Zhao, Sixue Chen, Xiuren Zhang
Summary: Phosphorylation can regulate miRNA metabolism by controlling the accumulation balance of SE and quickly clearing excessive SE.
Review
Biochemistry & Molecular Biology
Yu-Ting Weng, Yao-Ming Chang, Yijuang Chern
Summary: MicroRNAs (miRNAs) are 22-nucleotide noncoding RNAs that play important roles in cell differentiation, development, and function. They can affect gene expression and mediate communication between different types of cells. Dysregulation of miRNA biogenesis and sorting has been observed in neurodegenerative diseases, such as Huntington's disease, Parkinson's disease, amyotrophic lateral sclerosis, and Alzheimer's disease. Understanding the molecular mechanisms underlying dysregulated miRNAs in neurodegenerative diseases is crucial for diagnosis and therapeutic interventions.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Cell Biology
Seungjae Lee, David Jee, Sid Srivatsava, Acong Yang, Abhinav Ramidi, Renfu Shang, Diane Bortoamiol-Becet, Sebastien Pfeffer, Shuo Gu, Jiayu Wen, Eric C. Lai
Summary: Canonical miRNA hairpins are processed by Drosha and Dicer enzymes to form small RNAs loaded into Ago effector. Splicing generates unannotated hairpins, termed ssdRNAs, which associate with Dicer and/or Ago complexes. They contaminate the canonical miRNA pathway and are tailed by terminal nucleotidyltransferases. This intragenomic conflict constitutes a Red Queen's race.
Article
Multidisciplinary Sciences
Upendra Kumar Pradhan, Nitesh Kumar Sharma, Prakash Kumar, Ashwani Kumar, Sagar Gupta, Ravi Shankar
Summary: The formation and expression of mature miRNAs are highly controlled processes dependent on post-transcriptional regulatory events. Recent findings suggest involvement of various RNA binding proteins beyond Drosha/Dicer in miRNA processing, leading to the development of Bayesian causal networks that explain miRNA biogenesis and formation levels. These networks, combined with XGBoost machine learning system, quantitatively explain miRNA formation and profiles, offering accurate predictions without the need for separate miRNA profiling experiments.
Article
Biochemistry & Molecular Biology
Ajeet P. Singh, Huacheng Luo, Meghana Matur, Melanie A. Eshelman, Karina Hamamoto, Arati Sharma, Julia Lesperance, Suming Huang
Summary: MicroRNAs (miRNAs) and long noncoding RNAs (lncRNAs) play crucial roles in regulating gene expression. The interaction between HOTTIP lncRNA and miRNAs in leukemogenesis is not well understood. Research shows that HOTTIP directly controls certain miRNA genes, impacting HOX cluster miRNAs expression and promoting leukemogenesis through chromatin remodeling. Inactivation of HOTTIP or miR-196b induces apoptosis and affects FAS gene expression in AML cells.
Article
Biochemistry & Molecular Biology
Hebatalla Mohamed, Amna Ghith, Stephen G. Bell
Summary: The cytochrome P450 superfamily of enzymes can catalyse oxidation reactions and their heme and active site environment can be studied using UV-visible absorbance spectroscopy. The binding of imidazole and pyridine-based ligands to different forms of bacterial cytochrome P450 enzymes was evaluated using this technique. The majority of ligands interacted with the heme as expected, but differences in heme environment were observed across enzyme/ligand combinations.
JOURNAL OF INORGANIC BIOCHEMISTRY
(2023)
Review
Health Care Sciences & Services
Nicola Montemurro, Luca Ricciardi, Alba Scerrati, Giorgio Ippolito, Giorgio Lofrese, Sokol Trungu, Andrea Stoccoro
Summary: This study found that approximately 35.1% of individuals with 22q11.2 deletion syndrome develop scoliosis. By studying miRNAs in both 22q11.2DS and AIS, two shared miRNAs were identified, supporting the hypothesis of common molecular alterations in these two diseases.
JOURNAL OF PERSONALIZED MEDICINE
(2022)
Article
Biochemistry & Molecular Biology
Yoann Abel, Christophe Charron, Camille Virciglio, Valerie Bourguignon-Igel, Marc Quinternet, Marie-Eve Chagot, Marie-Cecile Robert, Celine Verheggen, Christiane Branlant, Edouard Bertrand, Xavier Manival, Bruno Charpentier, Mathieu Rederstorff
Summary: In this study, it was found that the R2TP component RPAP3 binds to TRBP rather than PACT, and the binding of TRBP to RPAP3 or Dicer is mutually exclusive. Additionally, it was discovered that AGO(1/2), TRBP, and Dicer are all sensitive to HSP90 inhibition, with increased sensitivity of TRBP in the absence of RPAP3. Furthermore, RPAP3 appears to impede miRNA activity, suggesting that the R2TP chaperone might sequester TRBP to regulate the miRNA pathway.
NUCLEIC ACIDS RESEARCH
(2022)
Article
Biochemistry & Molecular Biology
Mu Li, Huihui Yu, Bangjun Zhou, Lu Gan, Shengjun Li, Chi Zhang, Bin Yu
Summary: MicroRNAs (miRNAs) play important roles in regulating gene expression, and JANUS is a crucial factor in both miRNA accumulation and splicing process.
NUCLEIC ACIDS RESEARCH
(2023)
Article
Chemistry, Multidisciplinary
Indrajit Saha, Nimisha Ghosh, Dariusz Plewczynski
Summary: SARS-CoV-2 poses a great challenge to mankind due to its evolution and variants. Human miRNA may play a vital role in viral infection and can be used as a therapeutic approach to inhibit viral replication.
Article
Biochemistry & Molecular Biology
Xi Yang, Weiguo Dong, Wenqing Ren, Qiuxia Zhao, Feijie Wu, Yuke He
Summary: In plants, HYL1, a core component of the miRNA processing machinery, regulates miRNA-mediated mRNA translation in the cytoplasm by monitoring the distribution of AGO1 onto polysomes, binding to the mRNAs of target genes, and repressing their translation. This study uncovered another function of HYL1 and provides insight into the mechanism of plant gene regulation.
Article
Biology
Abhinandan M. Tripathi, Yael Admoni, Arie Fridrich, Magda Lewandowska, Joachim M. Surm, Reuven Aharoni, Yehu Moran
Summary: The biogenesis of miRNAs in animals and plants depends on RNase III Dicer, but their partner proteins are considered distinct. However, a homolog of the plant-specific Dicer partner HYL1 has been discovered in the cnidarian phylum, challenging the idea that miRNAs evolved convergently in animals and plants. This study shows that the HYL1 homolog Hyl1La is crucial for development and miRNA biogenesis in the cnidarian model Nematostella vectensis, suggesting an early emergence of the miRNA system.
Article
Biochemistry & Molecular Biology
Heike Braun, Michael Hauke, Anne Ripperger, Christian Ihling, Matthew Fuszard, Robert Eckenstaler, Ralf A. Benndorf
Summary: Knockdown of DICER1 or DROSHA by RNAi does not profoundly affect the angiogenic capacity of HUVEC, suggesting a potential increase in certain angiogenic activities instead. Further studies are needed to clarify the influence of these enzymes in the context of human endothelial cell-related angiogenesis.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Neurosciences
Neal D. Amin, Gokhan Senturk, Giancarlo Costaguta, Shawn Driscoll, Brendan O'Leary, Dario Bonanomi, Samuel L. Pfaff
Summary: This study found that microRNA (miRNA) can govern different gene network outputs at different expression levels, and miRNA-dependent phenotypes emerge at specific dose ranges due to hidden regulatory inflection points in their gene networks.
Article
Biochemistry & Molecular Biology
Renfu Shang, Dmitry A. Kretov, Scott Adamson, Thomas Treiber, Nora Treiber, Jeffrey Vedanayagam, Jeffrey H. Chuang, Gunter Meister, Daniel Cifuentes, Eric C. Lai
Summary: A study has revealed the important regulatory role of conserved loop sequences in the generation of miRNAs in vertebrates, particularly in the cleavage of pre-mir-144. This regulatory role involves the ILF3 complex and reshaping of the apical loop of pre-mir-144, allowing for cleavage by Dicer. Furthermore, these findings extend our understanding of nuclear and cytoplasmic regulatory events in miRNA maturation.
NUCLEIC ACIDS RESEARCH
(2022)